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Protein

Synaptotagmin-4

Gene

Syt4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis. Plays a role in dendrite formation by melanocytes.By similarity

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi184Calcium 1By similarity1
Metal bindingi184Calcium 2By similarity1
Metal bindingi191Calcium 1By similarity1
Metal bindingi245Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi246Calcium 1By similarity1
Metal bindingi246Calcium 2By similarity1
Metal bindingi246Calcium 3By similarity1
Metal bindingi249Calcium 3By similarity1
Metal bindingi252Calcium 2By similarity1
Metal bindingi252Calcium 3By similarity1

GO - Molecular functioni

GO - Biological processi

  • brain development Source: Ensembl
  • calcium ion-regulated exocytosis of neurotransmitter Source: GO_Central
  • memory Source: ParkinsonsUK-UCL
  • negative regulation of catecholamine secretion Source: MGI
  • negative regulation of dense core granule exocytosis Source: Ensembl
  • negative regulation of protein secretion Source: SynGO-UCL
  • negative regulation of retrograde trans-synaptic signaling by neuropeptide Source: SynGO-UCL
  • negative regulation of short-term neuronal synaptic plasticity Source: ParkinsonsUK-UCL
  • negative regulation of synaptic vesicle exocytosis Source: SynGO-UCL
  • negative regulation of vesicle fusion Source: Ensembl
  • neurotransmitter secretion Source: MGI
  • positive regulation of dendrite extension Source: MGI
  • positive regulation of dense core granule exocytosis Source: ParkinsonsUK-UCL
  • positive regulation of glutamate secretion Source: ParkinsonsUK-UCL
  • regulation of calcium ion-dependent exocytosis Source: ParkinsonsUK-UCL
  • regulation of dopamine secretion Source: ParkinsonsUK-UCL
  • regulation of endocytosis Source: ParkinsonsUK-UCL
  • regulation of vesicle fusion Source: ParkinsonsUK-UCL
  • secretory granule maturation Source: Ensembl
  • synaptic vesicle transport Source: UniProtKB
  • vesicle fusion with vesicle Source: Ensembl

Keywordsi

Biological processDifferentiation
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-4
Alternative name(s):
Synaptotagmin IV
Short name:
SytIV
Gene namesi
Name:Syt4
Synonyms:Syt3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:101759. Syt4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 16VesicularSequence analysisAdd BLAST16
Transmembranei17 – 37HelicalSequence analysisAdd BLAST21
Topological domaini38 – 425CytoplasmicSequence analysisAdd BLAST388

GO - Cellular componenti

  • astrocyte projection Source: ParkinsonsUK-UCL
  • axon Source: ParkinsonsUK-UCL
  • cell junction Source: UniProtKB-KW
  • dendrite Source: ParkinsonsUK-UCL
  • dense core granule Source: ParkinsonsUK-UCL
  • exocytic vesicle Source: Ensembl
  • Golgi apparatus Source: ParkinsonsUK-UCL
  • integral component of membrane Source: UniProtKB
  • intracellular membrane-bounded organelle Source: MGI
  • microvesicle Source: ParkinsonsUK-UCL
  • neuronal cell body Source: ParkinsonsUK-UCL
  • neuron projection Source: MGI
  • neuron projection terminus Source: Ensembl
  • perinuclear region of cytoplasm Source: ParkinsonsUK-UCL
  • plasma membrane Source: ParkinsonsUK-UCL
  • postsynapse Source: SynGO
  • presynapse Source: SynGO-UCL
  • secretory granule membrane Source: Ensembl
  • somatodendritic compartment Source: ParkinsonsUK-UCL
  • vesicle Source: ParkinsonsUK-UCL

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001839491 – 425Synaptotagmin-4Add BLAST425

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei135PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40749.
PaxDbiP40749.
PRIDEiP40749.

PTM databases

iPTMnetiP40749.
PhosphoSitePlusiP40749.

Expressioni

Tissue specificityi

Expressed in many regions of the nervous system but is undetectable in extra neural tissues (PubMed:8058779).1 Publication

Gene expression databases

BgeeiENSMUSG00000024261.
CleanExiMM_SYT3.
MM_SYT4.
GenevisibleiP40749. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025110.

Structurei

3D structure databases

ProteinModelPortaliP40749.
SMRiP40749.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini155 – 258C2 1PROSITE-ProRule annotationAdd BLAST104
Domaini289 – 392C2 2PROSITE-ProRule annotationAdd BLAST104

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni147 – 297Phospholipid bindingCuratedAdd BLAST151

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1028. Eukaryota.
ENOG410XRME. LUCA.
GeneTreeiENSGT00760000118973.
HOVERGENiHBG005010.
InParanoidiP40749.
KOiK19904.
OMAiQIAKWHM.
OrthoDBiEOG091G09ZN.
PhylomeDBiP40749.
TreeFamiTF315600.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiView protein in InterPro
IPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028683. SYT4.
PANTHERiPTHR10024:SF280. PTHR10024:SF280. 1 hit.
PfamiView protein in Pfam
PF00168. C2. 2 hits.
PRINTSiPR00399. SYNAPTOTAGMN.
SMARTiView protein in SMART
SM00239. C2. 2 hits.
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiView protein in PROSITE
PS50004. C2. 2 hits.

Sequencei

Sequence statusi: Complete.

P40749-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPITTSRVE FDEIPTVVGI FSAFGLVFTV SLFAWICCQR RSAKSNKTPP
60 70 80 90 100
YKFVHVLKGV DIYPENLSSK KKFGGDDKSE VKGKAALPNL SLHLDLEKRD
110 120 130 140 150
LNGNFPKANP KAGSSSDLEN VTPKLFTETE KEANSPESLK SSTSLTSEEK
160 170 180 190 200
QEKLGTLFLS LEYNFEKKAF VVNIKEAQGL PAMDEQSMTS DPYIKMTILP
210 220 230 240 250
EKKHRVKTRV LRKTLDPVFD ETFTFYGIPY PHIQELSLHF TVLSFDRFSR
260 270 280 290 300
DDVIGEVLIP LSGIELSDGK MLMTREIIKR NAKKSSGRGE LLVSLCYQST
310 320 330 340 350
TNTLTVVVLK ARHLPKSDVS GLSDPYVKVN LYHAKKRISK KKTHVKKCTP
360 370 380 390 400
NAVFNELFVF DIPCESLEEI SVEFLVLDSE RGSRNEVIGR LVLGATAEGS
410 420
GGGHWKEICD FPRRQIAKWH MLCDG
Length:425
Mass (Da):47,659
Last modified:July 11, 2003 - v2
Checksum:i9A01144898CDFCEB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti70K → Q in AAA20971 (PubMed:8058779).Curated1
Sequence conflicti85A → T in AAA20971 (PubMed:8058779).Curated1
Sequence conflicti220D → G in AAA20971 (PubMed:8058779).Curated1
Sequence conflicti246D → N in BAC31726 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10355 mRNA. Translation: AAA20971.1.
AK032106 mRNA. Translation: BAC27705.1.
AK043985 mRNA. Translation: BAC31726.1.
AK050515 mRNA. Translation: BAC34301.1.
AK083960 mRNA. Translation: BAC39080.1.
AK148681 mRNA. Translation: BAE28640.1.
BC058208 mRNA. Translation: AAH58208.1.
CCDSiCCDS29109.1.
RefSeqiNP_033334.2. NM_009308.3.
UniGeneiMm.233846.

Genome annotation databases

EnsembliENSMUST00000025110; ENSMUSP00000025110; ENSMUSG00000024261.
GeneIDi20983.
KEGGimmu:20983.
UCSCiuc008ehy.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSYT4_MOUSE
AccessioniPrimary (citable) accession number: P40749
Secondary accession number(s): Q3UFC1, Q8BGH3, Q8BRL6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 11, 2003
Last modified: July 5, 2017
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families