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Protein

Synaptotagmin-4

Gene

Syt4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis.

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi184 – 1841Calcium 1By similarity
Metal bindingi184 – 1841Calcium 2By similarity
Metal bindingi191 – 1911Calcium 1By similarity
Metal bindingi245 – 2451Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi246 – 2461Calcium 1By similarity
Metal bindingi246 – 2461Calcium 2By similarity
Metal bindingi246 – 2461Calcium 3By similarity
Metal bindingi249 – 2491Calcium 3By similarity
Metal bindingi252 – 2521Calcium 2By similarity
Metal bindingi252 – 2521Calcium 3By similarity

GO - Molecular functioni

  • calcium ion binding Source: MGI
  • clathrin binding Source: GO_Central
  • phosphatidylserine binding Source: Ensembl
  • syntaxin binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-4
Alternative name(s):
Synaptotagmin IV
Short name:
SytIV
Gene namesi
Name:Syt4
Synonyms:Syt3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:101759. Syt4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616VesicularSequence AnalysisAdd
BLAST
Transmembranei17 – 3721HelicalSequence AnalysisAdd
BLAST
Topological domaini38 – 425388CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • dense core granule Source: Ensembl
  • integral component of membrane Source: UniProtKB
  • intracellular membrane-bounded organelle Source: MGI
  • neuron projection Source: MGI
  • perinuclear region of cytoplasm Source: MGI
  • plasma membrane Source: MGI
  • synaptic vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 425425Synaptotagmin-4PRO_0000183949Add
BLAST

Proteomic databases

MaxQBiP40749.
PRIDEiP40749.

PTM databases

PhosphoSiteiP40749.

Expressioni

Tissue specificityi

Expressed in many regions of the nervous system but is undetectable in extra neural tissues.

Gene expression databases

BgeeiP40749.
CleanExiMM_SYT3.
MM_SYT4.
ExpressionAtlasiP40749. baseline and differential.
GenevisibleiP40749. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025110.

Structurei

3D structure databases

ProteinModelPortaliP40749.
SMRiP40749. Positions 152-425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini155 – 258104C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini289 – 392104C2 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni147 – 297151Phospholipid bindingCuratedAdd
BLAST

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated
Contains 2 C2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG292488.
GeneTreeiENSGT00760000118973.
HOVERGENiHBG005010.
InParanoidiP40749.
OMAiQIAKWHM.
OrthoDBiEOG75J0N2.
PhylomeDBiP40749.
TreeFamiTF315600.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028683. SYT4.
[Graphical view]
PANTHERiPTHR10024:SF114. PTHR10024:SF114. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40749-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPITTSRVE FDEIPTVVGI FSAFGLVFTV SLFAWICCQR RSAKSNKTPP
60 70 80 90 100
YKFVHVLKGV DIYPENLSSK KKFGGDDKSE VKGKAALPNL SLHLDLEKRD
110 120 130 140 150
LNGNFPKANP KAGSSSDLEN VTPKLFTETE KEANSPESLK SSTSLTSEEK
160 170 180 190 200
QEKLGTLFLS LEYNFEKKAF VVNIKEAQGL PAMDEQSMTS DPYIKMTILP
210 220 230 240 250
EKKHRVKTRV LRKTLDPVFD ETFTFYGIPY PHIQELSLHF TVLSFDRFSR
260 270 280 290 300
DDVIGEVLIP LSGIELSDGK MLMTREIIKR NAKKSSGRGE LLVSLCYQST
310 320 330 340 350
TNTLTVVVLK ARHLPKSDVS GLSDPYVKVN LYHAKKRISK KKTHVKKCTP
360 370 380 390 400
NAVFNELFVF DIPCESLEEI SVEFLVLDSE RGSRNEVIGR LVLGATAEGS
410 420
GGGHWKEICD FPRRQIAKWH MLCDG
Length:425
Mass (Da):47,659
Last modified:July 11, 2003 - v2
Checksum:i9A01144898CDFCEB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti70 – 701K → Q in AAA20971 (PubMed:8058779).Curated
Sequence conflicti85 – 851A → T in AAA20971 (PubMed:8058779).Curated
Sequence conflicti220 – 2201D → G in AAA20971 (PubMed:8058779).Curated
Sequence conflicti246 – 2461D → N in BAC31726 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10355 mRNA. Translation: AAA20971.1.
AK032106 mRNA. Translation: BAC27705.1.
AK043985 mRNA. Translation: BAC31726.1.
AK050515 mRNA. Translation: BAC34301.1.
AK083960 mRNA. Translation: BAC39080.1.
AK148681 mRNA. Translation: BAE28640.1.
BC058208 mRNA. Translation: AAH58208.1.
CCDSiCCDS29109.1.
RefSeqiNP_033334.2. NM_009308.3.
UniGeneiMm.233846.

Genome annotation databases

EnsembliENSMUST00000025110; ENSMUSP00000025110; ENSMUSG00000024261.
GeneIDi20983.
KEGGimmu:20983.
UCSCiuc008ehy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10355 mRNA. Translation: AAA20971.1.
AK032106 mRNA. Translation: BAC27705.1.
AK043985 mRNA. Translation: BAC31726.1.
AK050515 mRNA. Translation: BAC34301.1.
AK083960 mRNA. Translation: BAC39080.1.
AK148681 mRNA. Translation: BAE28640.1.
BC058208 mRNA. Translation: AAH58208.1.
CCDSiCCDS29109.1.
RefSeqiNP_033334.2. NM_009308.3.
UniGeneiMm.233846.

3D structure databases

ProteinModelPortaliP40749.
SMRiP40749. Positions 152-425.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025110.

PTM databases

PhosphoSiteiP40749.

Proteomic databases

MaxQBiP40749.
PRIDEiP40749.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025110; ENSMUSP00000025110; ENSMUSG00000024261.
GeneIDi20983.
KEGGimmu:20983.
UCSCiuc008ehy.1. mouse.

Organism-specific databases

CTDi6860.
MGIiMGI:101759. Syt4.

Phylogenomic databases

eggNOGiNOG292488.
GeneTreeiENSGT00760000118973.
HOVERGENiHBG005010.
InParanoidiP40749.
OMAiQIAKWHM.
OrthoDBiEOG75J0N2.
PhylomeDBiP40749.
TreeFamiTF315600.

Miscellaneous databases

NextBioi299976.
PROiP40749.
SOURCEiSearch...

Gene expression databases

BgeeiP40749.
CleanExiMM_SYT3.
MM_SYT4.
ExpressionAtlasiP40749. baseline and differential.
GenevisibleiP40749. MM.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028683. SYT4.
[Graphical view]
PANTHERiPTHR10024:SF114. PTHR10024:SF114. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain cortex, Medulla oblongata, Pancreas, Spinal ganglion and Sympathetic ganglion.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Eye.

Entry informationi

Entry nameiSYT4_MOUSE
AccessioniPrimary (citable) accession number: P40749
Secondary accession number(s): Q3UFC1, Q8BGH3, Q8BRL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 11, 2003
Last modified: July 22, 2015
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.