Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P40749 (SYT4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Synaptotagmin-4
Alternative name(s):
Synaptotagmin IV
Short name=SytIV
Gene names
Name:Syt4
Synonyms:Syt3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length425 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis.

Cofactor

Binds 3 calcium ions per subunit. The ions are bound to the C2 domains By similarity.

Subcellular location

Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane; Single-pass membrane protein.

Tissue specificity

Expressed in many regions of the nervous system but is undetectable in extra neural tissues.

Sequence similarities

Belongs to the synaptotagmin family.

Contains 2 C2 domains.

Ontologies

Keywords
   Cellular componentCell junction
Cytoplasmic vesicle
Membrane
Synapse
   DomainRepeat
Transmembrane
Transmembrane helix
   LigandCalcium
Metal-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processexocytosis

Inferred from electronic annotation. Source: InterPro

negative regulation of calcium ion-dependent exocytosis

Inferred from electronic annotation. Source: Ensembl

negative regulation of vesicle fusion

Inferred from electronic annotation. Source: Ensembl

neurotransmitter secretion

Inferred from direct assay PubMed 15197251. Source: MGI

regulation of calcium ion-dependent exocytosis

Traceable author statement Ref.1. Source: UniProtKB

synaptic vesicle transport

Traceable author statement Ref.1. Source: UniProtKB

   Cellular_componentcell junction

Inferred from electronic annotation. Source: UniProtKB-KW

dense core granule

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Traceable author statement Ref.1. Source: UniProtKB

neuron projection

Inferred from direct assay PubMed 15197251. Source: MGI

perinuclear region of cytoplasm

Inferred from direct assay PubMed 15197251. Source: MGI

synaptic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncalcium ion binding

Inferred from direct assay PubMed 15197251. Source: MGI

phosphatidylserine binding

Inferred from electronic annotation. Source: Ensembl

transporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 425425Synaptotagmin-4
PRO_0000183949

Regions

Topological domain1 – 1616Vesicular Potential
Transmembrane17 – 3721Helical; Potential
Topological domain38 – 425388Cytoplasmic Potential
Domain155 – 258104C2 1
Domain289 – 392104C2 2
Region147 – 297151Phospholipid binding Probable

Sites

Metal binding1841Calcium 1 By similarity
Metal binding1841Calcium 2 By similarity
Metal binding1911Calcium 1 By similarity
Metal binding2451Calcium 1; via carbonyl oxygen By similarity
Metal binding2461Calcium 1 By similarity
Metal binding2461Calcium 2 By similarity
Metal binding2461Calcium 3 By similarity
Metal binding2491Calcium 3 By similarity
Metal binding2521Calcium 2 By similarity
Metal binding2521Calcium 3 By similarity

Experimental info

Sequence conflict701K → Q in AAA20971. Ref.1
Sequence conflict851A → T in AAA20971. Ref.1
Sequence conflict2201D → G in AAA20971. Ref.1
Sequence conflict2461D → N in BAC31726. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P40749 [UniParc].

Last modified July 11, 2003. Version 2.
Checksum: 9A01144898CDFCEB

FASTA42547,659
        10         20         30         40         50         60 
MAPITTSRVE FDEIPTVVGI FSAFGLVFTV SLFAWICCQR RSAKSNKTPP YKFVHVLKGV 

        70         80         90        100        110        120 
DIYPENLSSK KKFGGDDKSE VKGKAALPNL SLHLDLEKRD LNGNFPKANP KAGSSSDLEN 

       130        140        150        160        170        180 
VTPKLFTETE KEANSPESLK SSTSLTSEEK QEKLGTLFLS LEYNFEKKAF VVNIKEAQGL 

       190        200        210        220        230        240 
PAMDEQSMTS DPYIKMTILP EKKHRVKTRV LRKTLDPVFD ETFTFYGIPY PHIQELSLHF 

       250        260        270        280        290        300 
TVLSFDRFSR DDVIGEVLIP LSGIELSDGK MLMTREIIKR NAKKSSGRGE LLVSLCYQST 

       310        320        330        340        350        360 
TNTLTVVVLK ARHLPKSDVS GLSDPYVKVN LYHAKKRISK KKTHVKKCTP NAVFNELFVF 

       370        380        390        400        410        420 
DIPCESLEEI SVEFLVLDSE RGSRNEVIGR LVLGATAEGS GGGHWKEICD FPRRQIAKWH 


MLCDG 

« Hide

References

« Hide 'large scale' references
[1]"A third synaptotagmin gene, Syt3, in the mouse."
Hilbush B.S., Morgan J.I.
Proc. Natl. Acad. Sci. U.S.A. 91:8195-8199(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Brain cortex, Medulla oblongata, Pancreas, Spinal ganglion and Sympathetic ganglion.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Eye.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U10355 mRNA. Translation: AAA20971.1.
AK032106 mRNA. Translation: BAC27705.1.
AK043985 mRNA. Translation: BAC31726.1.
AK050515 mRNA. Translation: BAC34301.1.
AK083960 mRNA. Translation: BAC39080.1.
AK148681 mRNA. Translation: BAE28640.1.
BC058208 mRNA. Translation: AAH58208.1.
CCDSCCDS29109.1.
RefSeqNP_033334.2. NM_009308.3.
UniGeneMm.233846.

3D structure databases

ProteinModelPortalP40749.
SMRP40749. Positions 152-425.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP40749.

Proteomic databases

PRIDEP40749.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025110; ENSMUSP00000025110; ENSMUSG00000024261.
GeneID20983.
KEGGmmu:20983.
UCSCuc008ehy.1. mouse.

Organism-specific databases

CTD6860.
MGIMGI:101759. Syt4.

Phylogenomic databases

eggNOGNOG292488.
GeneTreeENSGT00750000117274.
HOVERGENHBG005010.
InParanoidP40749.
OMAQIAKWHM.
OrthoDBEOG75J0N2.
PhylomeDBP40749.
TreeFamTF315600.

Gene expression databases

BgeeP40749.
CleanExMM_SYT3.
MM_SYT4.
GenevestigatorP40749.

Family and domain databases

Gene3D2.60.40.150. 2 hits.
InterProIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028683. SYT4.
[Graphical view]
PANTHERPTHR10024:SF114. PTHR10024:SF114. 1 hit.
PfamPF00168. C2. 2 hits.
[Graphical view]
PRINTSPR00399. SYNAPTOTAGMN.
SMARTSM00239. C2. 2 hits.
[Graphical view]
SUPFAMSSF49562. SSF49562. 2 hits.
PROSITEPS50004. C2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio299976.
PROP40749.
SOURCESearch...

Entry information

Entry nameSYT4_MOUSE
AccessionPrimary (citable) accession number: P40749
Secondary accession number(s): Q3UFC1, Q8BGH3, Q8BRL6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 11, 2003
Last modified: July 9, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot