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P40748 (SYT3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Synaptotagmin-3
Alternative name(s):
Synaptotagmin III
Short name=SytIII
Gene names
Name:Syt3
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length588 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis.

Cofactor

Binds 3 calcium ions per subunit. The ions are bound to the C2 domains By similarity.

Subunit structure

Homodimer. Can also form heterodimers with SYT6 By similarity.

Subcellular location

Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass membrane protein.

Tissue specificity

Brain, various endocrine tissues and hormone-secreting clonal cells.

Domain

The first C2 domain mediates Ca2+-dependent phospholipid binding.

Sequence similarities

Belongs to the synaptotagmin family.

Contains 2 C2 domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 588588Synaptotagmin-3
PRO_0000183947

Regions

Topological domain1 – 5454Vesicular Potential
Transmembrane55 – 7521Helical; Potential
Topological domain76 – 588513Cytoplasmic Potential
Domain299 – 400102C2 1
Domain431 – 534104C2 2

Sites

Metal binding3281Calcium 1 By similarity
Metal binding3281Calcium 2 By similarity
Metal binding3341Calcium 1 By similarity
Metal binding3861Calcium 1 By similarity
Metal binding3861Calcium 2 By similarity
Metal binding3871Calcium 1; via carbonyl oxygen By similarity
Metal binding3881Calcium 1 By similarity
Metal binding3881Calcium 2 By similarity
Metal binding3881Calcium 3 By similarity
Metal binding3911Calcium 3 By similarity
Metal binding3941Calcium 2 By similarity
Metal binding3941Calcium 3 By similarity

Experimental info

Sequence conflict2871T → S in AAK56959. Ref.2
Sequence conflict4451L → R in AAK56959. Ref.2

Secondary structure

1 ........................................... 588
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P40748 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 9D9D33CF5BAD8331

FASTA58863,313
        10         20         30         40         50         60 
MSGDYEDDLC RRALILVSDL CARIRDADTN DRCQEFNELR IRGYPRGPDA DISVSLLSVI 

        70         80         90        100        110        120 
VTFCGIVLLG VSLFVSWKLC WVPWRDKGGS AVGGGPLRKD LAPGVGLAGL VGGGGHHLGA 

       130        140        150        160        170        180 
SLGGHPLLGG PHHHAHPAHH PPFAELLEPG GLGGSEPPEP SYLDMDSYPE AAVASVVAAG 

       190        200        210        220        230        240 
VKPSQTSPEL PSEGGTGSGL LLLPPSGGGL PSAQSHQQVT SLAPTTRYPA LPRPLTQQTL 

       250        260        270        280        290        300 
TTQADPSSEE RPPALPLPLP GGEEKAKLIG QIKPELYQGT GPGGRRTGGG SGEAGAPCGR 

       310        320        330        340        350        360 
ISFALRYLYG SDQLVVRILQ ALDLPAKDSN GFSDPYVKIY LLPDRKKKFQ TKVHRKTLNP 

       370        380        390        400        410        420 
IFNETFQFSV PLAELAQRKL HFSVYDFDRF SRHDLIGQVV LDNLLELAEQ PPDRPLWRDI 

       430        440        450        460        470        480 
LEGGSEKADL GELNFSLCYL PTAGLLTVTI IKASNLKAMD LTGFSDPYVK ASLISEGRRL 

       490        500        510        520        530        540 
KKRKTSIKKN TLNPTYNEAL VFDVAPESVE NVGLSIAVVD YDCIGHNEVI GVCRVGPEAA 

       550        560        570        580 
DPHGREHWAE MLANPRKPVE HWHQLVEEKT LSSFTKGGKG LSEKENSE

« Hide

References

[1]"Synaptotagmin III is a novel isoform of rat synaptotagmin expressed in endocrine and neuronal cells."
Mizuta M., Inagaki N., Nemoto Y., Matsukura S., Takahashi M., Seino S.
J. Biol. Chem. 269:11675-11678(1994) [PubMed: 8163462] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
[2]Shin O.-H., Li C., Ullrich B., Zhang J.Z., Anderson R.G.W., Brose N., Suedhof T.C.
Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Crystal structure of the cytosolic C2A-C2B domains of synaptotagmin III. Implications for Ca(2+)-independent SNARE complex interaction."
Sutton R.B., Ernst J.A., Brunger A.T.
J. Cell Biol. 147:589-598(1999) [PubMed: 10545502] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 293-588.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D28512 mRNA. Translation: BAA05870.1.
AF375464 mRNA. Translation: AAK56959.1.
IPIIPI00192477.
PIRA53563.
RefSeqNP_061995.1. NM_019122.1.
UniGeneRn.48884.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1DQVX-ray3.20A293-588[»]
3HN8X-ray3.50A/B/C292-587[»]
ProteinModelPortalP40748.
SMRP40748. Positions 295-569.
ModBaseSearch...

Protein-protein interaction databases

IntActP40748. 2 interactions.
STRINGP40748.

PTM databases

PhosphoSiteP40748.

Proteomic databases

PRIDEP40748.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000026251; ENSRNOP00000026251; ENSRNOG00000019318.
GeneID25731.
KEGGrno:25731.
UCSCNM_019122. rat.

Organism-specific databases

CTD84258.
RGD3805. Syt3.

Phylogenomic databases

eggNOGKOG1028.
HOVERGENHBG005010.
InParanoidP40748.
OrthoDBEOG4SQWWQ.

Gene expression databases

ArrayExpressP40748.
GenevestigatorP40748.
GermOnlineENSRNOG00000019318. Rattus norvegicus.

Family and domain databases

InterProIPR000008. C2_Ca-dep.
IPR008973. C2_Ca/lipid-bd_dom_CaLB.
IPR020477. C2_dom.
IPR018029. C2_membr_targeting.
IPR001565. Synaptotagmin.
[Graphical view]
PfamPF00168. C2. 2 hits.
[Graphical view]
PRINTSPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTSM00239. C2. 2 hits.
[Graphical view]
SUPFAMSSF49562. C2_CaLB. 2 hits.
PROSITEPS50004. C2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio607859.

Entry information

Entry nameSYT3_RAT
AccessionPrimary (citable) accession number: P40748
Secondary accession number(s): Q925B7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: January 25, 2012
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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