Synaptotagmin-3 - Rattus norvegicus (Rat)

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Reviewed, UniProtKB/Swiss-Prot P40748 (SYT3_RAT)

Last modified January 19, 2010. Version 97. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Synaptotagmin-3
Alternative name(s):
    Synaptotagmin III
      Short name=SytIII

Gene names

Name:

Syt3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	May be involved in Ca²⁺-dependent exocytosis of secretory vesicles through Ca²⁺ and phospholipid binding to the C2 domain or may serve as Ca²⁺ sensors in the process of vesicular trafficking and exocytosis.
Cofactor	Binds 3 calcium ions per subunit. The ions are bound to the C2 domains By similarity.
Subunit structure	Homodimer. Can also form heterodimers By similarity.
Subcellular location	Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass membrane protein.
Tissue specificity	Brain, various endocrine tissues and hormone-secreting clonal cells.
Domain	The first C2 domain mediates Ca²⁺-dependent phospholipid binding.
Sequence similarities	Belongs to the synaptotagmin family. Contains 2 C2 domains.

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Ontologies

Keywords
Cellular component	Cell junction Cytoplasmic vesicle Membrane Synapse
Domain	Repeat Transmembrane
Ligand	Calcium Metal-binding
Technical term	3D-structure
Gene Ontology (GO)
Biological process	calcium ion-dependent exocytosis Traceable author statement. Source: RGD synaptic vesicle exocytosis Traceable author statement. Source: RGD
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell synaptic vesicle Inferred from electronic annotation. Source: InterPro
Molecular function	calcium ion binding Traceable author statement. Source: RGD calcium-dependent phospholipid binding Inferred from direct assay. Source: RGD syntaxin binding Inferred from direct assay. Source: RGD transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
Cacna1b	Q02294	1	EBI-458106,EBI-540038
Syt1	P21707	1	EBI-458106,EBI-458098

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 588

588

Synaptotagmin-3

PRO_0000183947

Regions

Topological domain

1 – 54

Vesicular Potential

Transmembrane

55 – 75

Potential

Topological domain

76 – 588

513

Cytoplasmic Potential

Domain

299 – 400

102

C2 1

Domain

431 – 534

104

C2 2

Sites

Metal binding

328

Calcium 1 By similarity

Metal binding

328

Calcium 2 By similarity

Metal binding

334

Calcium 1 By similarity

Metal binding

386

Calcium 1 By similarity

Metal binding

386

Calcium 2 By similarity

Metal binding

387

Calcium 1; via carbonyl oxygen By similarity

Metal binding

388

Calcium 1 By similarity

Metal binding

388

Calcium 2 By similarity

Metal binding

388

Calcium 3 By similarity

Metal binding

391

Calcium 3 By similarity

Metal binding

394

Calcium 2 By similarity

Metal binding

394

Calcium 3 By similarity

Experimental info

Sequence conflict

287

T → S in AAK56959. Ref.2

Sequence conflict

445

L → R in AAK56959. Ref.2

Secondary structure

588

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P40748-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 9D9D33CF5BAD8331
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FASTA

588

63,313

        10         20         30         40         50         60 
MSGDYEDDLC RRALILVSDL CARIRDADTN DRCQEFNELR IRGYPRGPDA DISVSLLSVI 

        70         80         90        100        110        120 
VTFCGIVLLG VSLFVSWKLC WVPWRDKGGS AVGGGPLRKD LAPGVGLAGL VGGGGHHLGA 

       130        140        150        160        170        180 
SLGGHPLLGG PHHHAHPAHH PPFAELLEPG GLGGSEPPEP SYLDMDSYPE AAVASVVAAG 

       190        200        210        220        230        240 
VKPSQTSPEL PSEGGTGSGL LLLPPSGGGL PSAQSHQQVT SLAPTTRYPA LPRPLTQQTL 

       250        260        270        280        290        300 
TTQADPSSEE RPPALPLPLP GGEEKAKLIG QIKPELYQGT GPGGRRTGGG SGEAGAPCGR 

       310        320        330        340        350        360 
ISFALRYLYG SDQLVVRILQ ALDLPAKDSN GFSDPYVKIY LLPDRKKKFQ TKVHRKTLNP 

       370        380        390        400        410        420 
IFNETFQFSV PLAELAQRKL HFSVYDFDRF SRHDLIGQVV LDNLLELAEQ PPDRPLWRDI 

       430        440        450        460        470        480 
LEGGSEKADL GELNFSLCYL PTAGLLTVTI IKASNLKAMD LTGFSDPYVK ASLISEGRRL 

       490        500        510        520        530        540 
KKRKTSIKKN TLNPTYNEAL VFDVAPESVE NVGLSIAVVD YDCIGHNEVI GVCRVGPEAA 

       550        560        570        580 
DPHGREHWAE MLANPRKPVE HWHQLVEEKT LSSFTKGGKG LSEKENSE

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References

[1]	"Synaptotagmin III is a novel isoform of rat synaptotagmin expressed in endocrine and neuronal cells." Mizuta M., Inagaki N., Nemoto Y., Matsukura S., Takahashi M., Seino S. J. Biol. Chem. 269:11675-11678(1994) [PubMed: 8163462] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[2]	Shin O.-H., Li C., Ullrich B., Zhang J.Z., Anderson R.G.W., Brose N., Suedhof T.C. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Crystal structure of the cytosolic C2A-C2B domains of synaptotagmin III. Implications for Ca(2+)-independent SNARE complex interaction." Sutton R.B., Ernst J.A., Brunger A.T. J. Cell Biol. 147:589-598(1999) [PubMed: 10545502] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 293-588.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D28512 mRNA. Translation: BAA05870.1.
AF375464 mRNA. Translation: AAK56959.1.

IPI

IPI00192477.

PIR

A53563.

RefSeq

NP_061995.1.

UniGene

Rn.48884

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DQV	X-ray	3.20	A	293-588	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P40748. 3 interactions.

STRING

P40748.

PTM databases

PhosphoSite

P40748.

Proteomic databases

PRIDE

P40748.

Genome annotation databases

Ensembl

ENSRNOT00000026251; ENSRNOP00000026251; ENSRNOG00000019318; Rattus norvegicus. [Genome view]

GeneID

25731.

KEGG

rno:25731.

UCSC

NM_019122. rat.

Organism-specific databases

CTD

25731.

RGD

3805. Syt3.

Phylogenomic databases

eggNOG

KOG1028.

HOVERGEN

P40748.

InParanoid

P40748.

Gene expression databases

ArrayExpress

P40748.

Genevestigator

P40748.

GermOnline

ENSRNOG00000019318. Rattus norvegicus.

Family and domain databases

InterPro

IPR000008. C2_Ca-dep.
IPR008973. C2_Ca/lipid-bd_dom_CaLB.
IPR018029. C2_membr_targeting.
IPR020477. C2_region.
IPR001565. Synaptotagmin.
[Graphical view]

Pfam

PF00168. C2. 2 hits.
[Graphical view]

PRINTS

PR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.

SMART

SM00239. C2. 2 hits.
[Graphical view]

PROSITE

PS50004. C2. 2 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

607859.

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Entry information

Entry name

SYT3_RAT

Accession

Primary (citable) accession number: P40748
Secondary accession number(s): Q925B7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	January 19, 2010
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents