Reviewed, UniProtKB/Swiss-Prot P40746 (TGL_BACSU)

Names and origin

Protein names

Recommended name:
    Protein-glutamine gamma-glutamyltransferase
    EC=2.3.2.13
Alternative name(s):
    Transglutaminase
      Short name=TGase

Gene names

Name:	tgl
Synonyms:	yugV, yuxF
Ordered Locus Names:	BSU31270

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probably plays a role in the assembly of the spore coat proteins by catalyzing epsilon-(gamma-glutamyl)lysine cross-links. Mediates the cross-linking of gerQ, probably in cooperation with yabG.
Catalytic activity	Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH(3).
Developmental stage	Expressed during sporulation.
Miscellaneous	In wild-type spores at 37 degrees Celsius, tgl mediates the cross-linking of gerQ in higher molecular mass forms. Some gerQ multimers are found in tgl mutant spores, indicating that tgl is not essential. Heat treatment for 20 minutes at 60 degrees Celsius, which maximally activates the tgl enzymatic activity, causes cross-linking of gerQ in isolated yabG spores but not in tgl/yabG double-mutant spores. Additionally, the germination frequency of the tgl/yabG double-mutant spores in the presence of L-alanine with or without heat activation at 60 degrees Celsius is lower that of wild-type spores.
Sequence similarities	Belongs to the bacillus TGase family.

Ontologies

Keywords
Biological process	Sporulation
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: HAMAP
Molecular function	acyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-glutamine gamma-glutamyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Protein-glutamine gamma-glutamyltransferase		PRO_0000213725

Sequences

	Sequence		Length	Mass (Da)
	P40746-1 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 2FB36C0F83DF162C	FASTA	245	28,296
	10 20 30 40 50 60 MIIVSGQLLR PQDIENWQID QDLNPLLKEM IETPVQFDYH SIAELMFELK LRMNIVAAAK 70 80 90 100 110 120 TLHKSGAKFA TFLKTYGNTT YWRVSPEGAL ELKYRMPPSK AIRDIAENGP FYAFECATAI 130 140 150 160 170 180 VIIYYLALID TIGEDKFNAS FDRIILYDWH YEKLPIYTET GHHFFLGDCL YFKNPEFDPQ 190 200 210 220 230 240 KAQWRGENVI LLGEDKYFAH GLGILNGKQI IDKLNSFRKK GALQSAYLLS QATRLDVPSL FRIVR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai)." Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H., Duesterhoeft A., Pohl T.M., Weitzenegger T. Microbiology 143:2769-2774(1997) [PubMed: 9274030] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"Cloning and characterization of genes encoding methyl-accepting chemotaxis proteins in Bacillus subtilis." Hanlon D.W., Ordal G.W. J. Biol. Chem. 269:14038-14046(1994) [PubMed: 8188684] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22. Strain: 168 / OI1085.
[4]	"Molecular cloning of the transglutaminase gene from Bacillus subtilis and its expression in Escherichia coli." Kobayashi K., Hashiguchi K., Yokozeki K., Yamanaka S. Biosci. Biotechnol. Biochem. 62:1109-1114(1998) [PubMed: 9692191] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Transglutaminase in sporulating cells of Bacillus subtilis." Kobayashi K., Suzuki S.I., Izawa Y., Miwa K., Yamanaka S. J. Gen. Appl. Microbiol. 44:85-91(1998) [PubMed: 12501297] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Modification of GerQ reveals a functional relationship between Tgl and YabG in the coat of Bacillus subtilis spores." Kuwana R., Okuda N., Takamatsu H., Watabe K. J. Biochem. 139:887-901(2006) [PubMed: 16751597] [Abstract] Cited for: RELATIONSHIP BETWEEN YABG AND TGL.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z93935 Genomic DNA. Translation: CAB07928.1. Z99119 Genomic DNA. Translation: CAB15105.1. L29189 Genomic DNA. Translation: AAA20553.1.
PIR	JE0179.
RefSeq	NP_391005.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	937158.
GenomeReviews	Gene locus BSU31270 in contig AL009126_GR.
KEGG	bsu:BSU31270.
NMPDR	fig|224308.1.peg.3130.
Organism-specific databases
SubtiList	BG10946. tgl. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P40746.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU3122-MON.
Family and domain databases
HAMAP	MF_00727. [Tree]
ProtoNet	Search...

Entry information

Entry name

TGL_BACSU

Accession

Primary (citable) accession number: P40746

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	July 15, 1998
Last modified:	November 25, 2008
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)