Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein-glutamine gamma-glutamyltransferase

Gene

tgl

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probably plays a role in the assembly of the spore coat proteins by catalyzing epsilon-(gamma-glutamyl)lysine cross-links. In wild-type spores at 37 degrees Celsius, tgl mediates the cross-linking of GerQ in higher molecular mass forms, probably in cooperation with YabG.

Catalytic activityi

Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Sporulation

Enzyme and pathway databases

BioCyciBSUB:BSU31270-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-glutamine gamma-glutamyltransferase (EC:2.3.2.13)
Alternative name(s):
Transglutaminase
Short name:
TGase
Gene namesi
Name:tgl
Synonyms:yugV, yuxF
Ordered Locus Names:BSU31270
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570 Componenti: Chromosome

Organism-specific databases

GenoListiBSU31270. [Micado]

Pathology & Biotechi

Disruption phenotypei

In cells missing this gene some GerQ multimers are present, indicating that tgl is not essential. Heat treatment for 20 minutes at 60 degrees Celsius, which maximally activates the Tgl enzymatic activity, causes cross-linking of GerQ in isolated yabG-deleted spores but not in tgl/yabG double-mutant spores. Additionally, the germination frequency of the tgl/yabG double-mutant spores in the presence of L-alanine with or without heat activation at 60 degrees Celsius is lower than that of wild-type spores.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245Protein-glutamine gamma-glutamyltransferasePRO_0000213725Add
BLAST

Proteomic databases

PaxDbiP40746.

Expressioni

Developmental stagei

Expressed during sporulation.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU31270.

Structurei

3D structure databases

ProteinModelPortaliP40746.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the bacillus TGase family.Curated

Phylogenomic databases

eggNOGiNOG250072.
HOGENOMiHOG000262157.
InParanoidiP40746.
KOiK00686.
OMAiYAFECAT.
OrthoDBiEOG6KQ6CT.

Family and domain databases

HAMAPiMF_00727. Tgl.
InterProiIPR020916. Gln_gamma-glutamylTfrase_bac.
[Graphical view]
ProDomiPD119415. PD119415. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

P40746-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIIVSGQLLR PQDIENWQID QDLNPLLKEM IETPVQFDYH SIAELMFELK
60 70 80 90 100
LRMNIVAAAK TLHKSGAKFA TFLKTYGNTT YWRVSPEGAL ELKYRMPPSK
110 120 130 140 150
AIRDIAENGP FYAFECATAI VIIYYLALID TIGEDKFNAS FDRIILYDWH
160 170 180 190 200
YEKLPIYTET GHHFFLGDCL YFKNPEFDPQ KAQWRGENVI LLGEDKYFAH
210 220 230 240
GLGILNGKQI IDKLNSFRKK GALQSAYLLS QATRLDVPSL FRIVR
Length:245
Mass (Da):28,296
Last modified:July 15, 1998 - v2
Checksum:i2FB36C0F83DF162C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z93935 Genomic DNA. Translation: CAB07928.1.
AL009126 Genomic DNA. Translation: CAB15105.1.
L29189 Genomic DNA. Translation: AAA20553.1.
PIRiJE0179.
RefSeqiNP_391005.1. NC_000964.3.
WP_003243977.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15105; CAB15105; BSU31270.
GeneIDi937158.
KEGGibsu:BSU31270.
PATRICi18978212. VBIBacSub10457_3269.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z93935 Genomic DNA. Translation: CAB07928.1.
AL009126 Genomic DNA. Translation: CAB15105.1.
L29189 Genomic DNA. Translation: AAA20553.1.
PIRiJE0179.
RefSeqiNP_391005.1. NC_000964.3.
WP_003243977.1. NZ_JNCM01000033.1.

3D structure databases

ProteinModelPortaliP40746.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU31270.

Proteomic databases

PaxDbiP40746.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15105; CAB15105; BSU31270.
GeneIDi937158.
KEGGibsu:BSU31270.
PATRICi18978212. VBIBacSub10457_3269.

Organism-specific databases

GenoListiBSU31270. [Micado]

Phylogenomic databases

eggNOGiNOG250072.
HOGENOMiHOG000262157.
InParanoidiP40746.
KOiK00686.
OMAiYAFECAT.
OrthoDBiEOG6KQ6CT.

Enzyme and pathway databases

BioCyciBSUB:BSU31270-MONOMER.

Family and domain databases

HAMAPiMF_00727. Tgl.
InterProiIPR020916. Gln_gamma-glutamylTfrase_bac.
[Graphical view]
ProDomiPD119415. PD119415. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai)."
    Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H., Duesterhoeft A., Pohl T.M., Weitzenegger T.
    Microbiology 143:2769-2774(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "Cloning and characterization of genes encoding methyl-accepting chemotaxis proteins in Bacillus subtilis."
    Hanlon D.W., Ordal G.W.
    J. Biol. Chem. 269:14038-14046(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22.
    Strain: 168 / OI1085.
  4. "Molecular cloning of the transglutaminase gene from Bacillus subtilis and its expression in Escherichia coli."
    Kobayashi K., Hashiguchi K., Yokozeki K., Yamanaka S.
    Biosci. Biotechnol. Biochem. 62:1109-1114(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Transglutaminase in sporulating cells of Bacillus subtilis."
    Kobayashi K., Suzuki S.I., Izawa Y., Miwa K., Yamanaka S.
    J. Gen. Appl. Microbiol. 44:85-91(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Modification of GerQ reveals a functional relationship between Tgl and YabG in the coat of Bacillus subtilis spores."
    Kuwana R., Okuda N., Takamatsu H., Watabe K.
    J. Biochem. 139:887-901(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: RELATIONSHIP BETWEEN YABG AND TGL, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiTGL_BACSU
AccessioniPrimary (citable) accession number: P40746
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 15, 1998
Last modified: May 27, 2015
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.