Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein-glutamine gamma-glutamyltransferase

Gene

tgl

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probably plays a role in the assembly of the spore coat proteins by catalyzing epsilon-(gamma-glutamyl)lysine cross-links. In wild-type spores at 37 degrees Celsius, tgl mediates the cross-linking of GerQ in higher molecular mass forms, probably in cooperation with YabG.

Catalytic activityi

Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Sporulation

Enzyme and pathway databases

BioCyciBSUB:BSU31270-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-glutamine gamma-glutamyltransferase (EC:2.3.2.13)
Alternative name(s):
Transglutaminase
Short name:
TGase
Gene namesi
Name:tgl
Synonyms:yugV, yuxF
Ordered Locus Names:BSU31270
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

In cells missing this gene some GerQ multimers are present, indicating that tgl is not essential. Heat treatment for 20 minutes at 60 degrees Celsius, which maximally activates the Tgl enzymatic activity, causes cross-linking of GerQ in isolated yabG-deleted spores but not in tgl/yabG double-mutant spores. Additionally, the germination frequency of the tgl/yabG double-mutant spores in the presence of L-alanine with or without heat activation at 60 degrees Celsius is lower than that of wild-type spores.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245Protein-glutamine gamma-glutamyltransferasePRO_0000213725Add
BLAST

Proteomic databases

PaxDbiP40746.

Expressioni

Developmental stagei

Expressed during sporulation.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100016996.

Structurei

Secondary structure

1
245
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 43Combined sources
Beta strandi7 – 93Combined sources
Helixi21 – 233Combined sources
Helixi24 – 329Combined sources
Beta strandi33 – 353Combined sources
Beta strandi37 – 415Combined sources
Helixi42 – 6524Combined sources
Helixi72 – 743Combined sources
Turni79 – 813Combined sources
Beta strandi82 – 843Combined sources
Beta strandi90 – 923Combined sources
Helixi98 – 10710Combined sources
Helixi109 – 1113Combined sources
Helixi116 – 13217Combined sources
Helixi134 – 1407Combined sources
Beta strandi145 – 1473Combined sources
Beta strandi150 – 1534Combined sources
Beta strandi156 – 1605Combined sources
Beta strandi169 – 1735Combined sources
Helixi182 – 1843Combined sources
Beta strandi185 – 1939Combined sources
Beta strandi196 – 1994Combined sources
Turni200 – 2023Combined sources
Beta strandi203 – 2053Combined sources
Helixi207 – 2159Combined sources
Beta strandi230 – 2356Combined sources
Helixi237 – 2448Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4P8IX-ray1.85A/B1-245[»]
4PA5X-ray1.86A/B1-245[»]
ProteinModelPortaliP40746.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the bacillus TGase family.Curated

Phylogenomic databases

HOGENOMiHOG000262157.
InParanoidiP40746.
KOiK00686.
OMAiGALELKY.

Family and domain databases

HAMAPiMF_00727. Tgl. 1 hit.
InterProiIPR020916. Gln_gamma-glutamylTfrase_bac.
[Graphical view]
ProDomiPD119415. PD119415. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

P40746-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIIVSGQLLR PQDIENWQID QDLNPLLKEM IETPVQFDYH SIAELMFELK
60 70 80 90 100
LRMNIVAAAK TLHKSGAKFA TFLKTYGNTT YWRVSPEGAL ELKYRMPPSK
110 120 130 140 150
AIRDIAENGP FYAFECATAI VIIYYLALID TIGEDKFNAS FDRIILYDWH
160 170 180 190 200
YEKLPIYTET GHHFFLGDCL YFKNPEFDPQ KAQWRGENVI LLGEDKYFAH
210 220 230 240
GLGILNGKQI IDKLNSFRKK GALQSAYLLS QATRLDVPSL FRIVR
Length:245
Mass (Da):28,296
Last modified:July 15, 1998 - v2
Checksum:i2FB36C0F83DF162C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z93935 Genomic DNA. Translation: CAB07928.1.
AL009126 Genomic DNA. Translation: CAB15105.1.
L29189 Genomic DNA. Translation: AAA20553.1.
PIRiJE0179.
RefSeqiNP_391005.1. NC_000964.3.
WP_003243977.1. NZ_JNCM01000033.1.

Genome annotation databases

EnsemblBacteriaiCAB15105; CAB15105; BSU31270.
GeneIDi937158.
KEGGibsu:BSU31270.
PATRICi18978212. VBIBacSub10457_3269.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z93935 Genomic DNA. Translation: CAB07928.1.
AL009126 Genomic DNA. Translation: CAB15105.1.
L29189 Genomic DNA. Translation: AAA20553.1.
PIRiJE0179.
RefSeqiNP_391005.1. NC_000964.3.
WP_003243977.1. NZ_JNCM01000033.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4P8IX-ray1.85A/B1-245[»]
4PA5X-ray1.86A/B1-245[»]
ProteinModelPortaliP40746.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100016996.

Proteomic databases

PaxDbiP40746.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15105; CAB15105; BSU31270.
GeneIDi937158.
KEGGibsu:BSU31270.
PATRICi18978212. VBIBacSub10457_3269.

Phylogenomic databases

HOGENOMiHOG000262157.
InParanoidiP40746.
KOiK00686.
OMAiGALELKY.

Enzyme and pathway databases

BioCyciBSUB:BSU31270-MONOMER.

Family and domain databases

HAMAPiMF_00727. Tgl. 1 hit.
InterProiIPR020916. Gln_gamma-glutamylTfrase_bac.
[Graphical view]
ProDomiPD119415. PD119415. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiTGL_BACSU
AccessioniPrimary (citable) accession number: P40746
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 15, 1998
Last modified: September 7, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.