P40746 (TGL_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-glutamine gamma-glutamyltransferase
EC=2.3.2.13

Alternative name(s):
Transglutaminase
Short name=TGase

Gene names

Name:	tgl
Synonyms:	yugV, yuxF
Ordered Locus Names:	BSU31270

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably plays a role in the assembly of the spore coat proteins by catalyzing epsilon-(gamma-glutamyl)lysine cross-links. In wild-type spores at 37 degrees Celsius, tgl mediates the cross-linking of gerQ in higher molecular mass forms, probably in cooperation with yabG. HAMAP MF_00727
Catalytic activity	Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3. HAMAP MF_00727
Developmental stage	Expressed during sporulation. HAMAP MF_00727
Disruption phenotype	In cells missing this gene some gerQ multimers are present, indicating that tgl is not essential. Heat treatment for 20 minutes at 60 degrees Celsius, which maximally activates the tgl enzymatic activity, causes cross-linking of gerQ in isolated yabG-deleted spores but not in tgl/yabG double-mutant spores. Additionally, the germination frequency of the tgl/yabG double- mutant spores in the presence of L-alanine with or without heat activation at 60 degrees Celsius is lower than that of wild-type spores. Ref.6
Sequence similarities	Belongs to the bacillus TGase family.

Ontologies

Keywords
Biological process	Sporulation
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein-glutamine gamma-glutamyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Protein-glutamine gamma-glutamyltransferase HAMAP MF_00727		PRO_0000213725

Sequences

	Sequence		Length	Mass (Da)
	P40746 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 2FB36C0F83DF162C	FASTA	245	28,296
	10 20 30 40 50 60 MIIVSGQLLR PQDIENWQID QDLNPLLKEM IETPVQFDYH SIAELMFELK LRMNIVAAAK 70 80 90 100 110 120 TLHKSGAKFA TFLKTYGNTT YWRVSPEGAL ELKYRMPPSK AIRDIAENGP FYAFECATAI 130 140 150 160 170 180 VIIYYLALID TIGEDKFNAS FDRIILYDWH YEKLPIYTET GHHFFLGDCL YFKNPEFDPQ 190 200 210 220 230 240 KAQWRGENVI LLGEDKYFAH GLGILNGKQI IDKLNSFRKK GALQSAYLLS QATRLDVPSL FRIVR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai)." Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H., Duesterhoeft A., Pohl T.M., Weitzenegger T. Microbiology 143:2769-2774(1997) [PubMed: 9274030] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"Cloning and characterization of genes encoding methyl-accepting chemotaxis proteins in Bacillus subtilis." Hanlon D.W., Ordal G.W. J. Biol. Chem. 269:14038-14046(1994) [PubMed: 8188684] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22. Strain: 168 / OI1085.
[4]	"Molecular cloning of the transglutaminase gene from Bacillus subtilis and its expression in Escherichia coli." Kobayashi K., Hashiguchi K., Yokozeki K., Yamanaka S. Biosci. Biotechnol. Biochem. 62:1109-1114(1998) [PubMed: 9692191] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Transglutaminase in sporulating cells of Bacillus subtilis." Kobayashi K., Suzuki S.I., Izawa Y., Miwa K., Yamanaka S. J. Gen. Appl. Microbiol. 44:85-91(1998) [PubMed: 12501297] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Modification of GerQ reveals a functional relationship between Tgl and YabG in the coat of Bacillus subtilis spores." Kuwana R., Okuda N., Takamatsu H., Watabe K. J. Biochem. 139:887-901(2006) [PubMed: 16751597] [Abstract] Cited for: RELATIONSHIP BETWEEN YABG AND TGL, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z93935 Genomic DNA. Translation: CAB07928.1. AL009126 Genomic DNA. Translation: CAB15105.1. L29189 Genomic DNA. Translation: AAA20553.1.
PIR	JE0179.
RefSeq	NP_391005.1. NC_000964.3.
3D structure databases
ProteinModelPortal	P40746.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000001223; EBBACP00000001223; EBBACG00000001221.
GeneID	937158.
GenomeReviews	Gene locus BSU31270 in contig AL009126_GR.
KEGG	bsu:BSU31270.
NMPDR	fig|224308.1.peg.3130.
PATRIC	18978212. VBIBacSub10457_3269.
Organism-specific databases
GenoList	BSU31270. [Micado]
Phylogenomic databases
GeneTree	EBGT00050000001129.
HOGENOM	HBG339895.
OMA	FECATAI.
ProtClustDB	PRK03187.
Enzyme and pathway databases
BioCyc	BSUB:BSU31270-MONOMER.
Family and domain databases
HAMAP	MF_00727. Tgl. [Tree]
InterPro	IPR020916. Gln_gamma-glutamylTfrase_bac. [Graphical view]
KO	K00686.
ProDom	PD119415. PD119415. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

TGL_BACSU

Accession

Primary (citable) accession number: P40746

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	July 15, 1998
Last modified:	January 25, 2012
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program