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P40746 (TGL_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein-glutamine gamma-glutamyltransferase

EC=2.3.2.13
Alternative name(s):
Transglutaminase
Short name=TGase
Gene names
Name:tgl
Synonyms:yugV, yuxF
Ordered Locus Names:BSU31270
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length245 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probably plays a role in the assembly of the spore coat proteins by catalyzing epsilon-(gamma-glutamyl)lysine cross-links. In wild-type spores at 37 degrees Celsius, tgl mediates the cross-linking of GerQ in higher molecular mass forms, probably in cooperation with YabG. HAMAP-Rule MF_00727

Catalytic activity

Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3. HAMAP-Rule MF_00727

Developmental stage

Expressed during sporulation. HAMAP-Rule MF_00727

Disruption phenotype

In cells missing this gene some GerQ multimers are present, indicating that tgl is not essential. Heat treatment for 20 minutes at 60 degrees Celsius, which maximally activates the Tgl enzymatic activity, causes cross-linking of GerQ in isolated yabG-deleted spores but not in tgl/yabG double-mutant spores. Additionally, the germination frequency of the tgl/yabG double-mutant spores in the presence of L-alanine with or without heat activation at 60 degrees Celsius is lower than that of wild-type spores. Ref.6

Sequence similarities

Belongs to the bacillus TGase family.

Ontologies

Keywords
   Biological processSporulation
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processsporulation resulting in formation of a cellular spore

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionprotein-glutamine gamma-glutamyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 245245Protein-glutamine gamma-glutamyltransferase HAMAP-Rule MF_00727
PRO_0000213725

Sequences

Sequence LengthMass (Da)Tools
P40746 [UniParc].

Last modified July 15, 1998. Version 2.
Checksum: 2FB36C0F83DF162C

FASTA24528,296
        10         20         30         40         50         60 
MIIVSGQLLR PQDIENWQID QDLNPLLKEM IETPVQFDYH SIAELMFELK LRMNIVAAAK 

        70         80         90        100        110        120 
TLHKSGAKFA TFLKTYGNTT YWRVSPEGAL ELKYRMPPSK AIRDIAENGP FYAFECATAI 

       130        140        150        160        170        180 
VIIYYLALID TIGEDKFNAS FDRIILYDWH YEKLPIYTET GHHFFLGDCL YFKNPEFDPQ 

       190        200        210        220        230        240 
KAQWRGENVI LLGEDKYFAH GLGILNGKQI IDKLNSFRKK GALQSAYLLS QATRLDVPSL 


FRIVR 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai)."
Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H., Duesterhoeft A., Pohl T.M., Weitzenegger T.
Microbiology 143:2769-2774(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Cloning and characterization of genes encoding methyl-accepting chemotaxis proteins in Bacillus subtilis."
Hanlon D.W., Ordal G.W.
J. Biol. Chem. 269:14038-14046(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22.
Strain: 168 / OI1085.
[4]"Molecular cloning of the transglutaminase gene from Bacillus subtilis and its expression in Escherichia coli."
Kobayashi K., Hashiguchi K., Yokozeki K., Yamanaka S.
Biosci. Biotechnol. Biochem. 62:1109-1114(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Transglutaminase in sporulating cells of Bacillus subtilis."
Kobayashi K., Suzuki S.I., Izawa Y., Miwa K., Yamanaka S.
J. Gen. Appl. Microbiol. 44:85-91(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Modification of GerQ reveals a functional relationship between Tgl and YabG in the coat of Bacillus subtilis spores."
Kuwana R., Okuda N., Takamatsu H., Watabe K.
J. Biochem. 139:887-901(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: RELATIONSHIP BETWEEN YABG AND TGL, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z93935 Genomic DNA. Translation: CAB07928.1.
AL009126 Genomic DNA. Translation: CAB15105.1.
L29189 Genomic DNA. Translation: AAA20553.1.
PIRJE0179.
RefSeqNP_391005.1. NC_000964.3.

3D structure databases

ProteinModelPortalP40746.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU31270.

Proteomic databases

PaxDbP40746.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB15105; CAB15105; BSU31270.
GeneID937158.
KEGGbsu:BSU31270.
PATRIC18978212. VBIBacSub10457_3269.

Organism-specific databases

GenoListBSU31270. [Micado]

Phylogenomic databases

eggNOGNOG250072.
HOGENOMHOG000262157.
KOK00686.
OMAYAFECAT.
OrthoDBEOG6KQ6CT.
ProtClustDBPRK03187.

Enzyme and pathway databases

BioCycBSUB:BSU31270-MONOMER.

Family and domain databases

HAMAPMF_00727. Tgl.
InterProIPR020916. Gln_gamma-glutamylTfrase_bac.
[Graphical view]
ProDomPD119415. PD119415. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Entry information

Entry nameTGL_BACSU
AccessionPrimary (citable) accession number: P40746
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 15, 1998
Last modified: April 16, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList