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P40740

- BGLH_BACSU

UniProt

P40740 - BGLH_BACSU

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Protein

Aryl-phospho-beta-D-glucosidase BglH

Gene

bglH

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of aryl-phospho-beta-D-glucosides such as 4-methylumbelliferyl-phospho-beta-D-glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Plays a major role in the utilization of arbutin or salicin as the sole carbon source. BglA and BglH are the major proteins contributing to hydrolysis of MUG-P by extracts of late-exponential-phase or stationary-phase B.subtilis cells.1 Publication

Catalytic activityi

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei175 – 1751Proton donorSequence Analysis
Active sitei368 – 3681NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

  1. 6-phospho-beta-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciBSUB:BSU39260-MONOMER.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Aryl-phospho-beta-D-glucosidase BglH (EC:3.2.1.86)
Alternative name(s):
6-phospho-beta-glucosidase
Gene namesi
Name:bglH
Ordered Locus Names:BSU39260
ORF Names:N17D
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU39260. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Aryl-phospho-beta-D-glucosidase BglHPRO_0000063873Add
BLAST

Proteomic databases

PaxDbiP40740.

Expressioni

Developmental stagei

Significantly expressed throughout all stages of growth or development.1 Publication

Inductioni

Highly up-regulated by aryl-beta-D-glucosides such as salicin or 4-methylumbelliferyl-beta-D-glucopyranoside (MUG).1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU39260.

Structurei

3D structure databases

ProteinModelPortaliP40740.
SMRiP40740. Positions 1-469.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088631.
InParanoidiP40740.
KOiK01223.
OMAiNAMYQAA.
OrthoDBiEOG6F81PM.
PhylomeDBiP40740.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40740-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSNEKRFPE GFLWGGAVAA NQVEGAYNEG GKGLSTADVS PNGIMSPFDE
60 70 80 90 100
SMTSLNLYHN GIDFYHRYKE DIALFAEMGF KAFRTSIAWT RIFPNGDEEE
110 120 130 140 150
PNEEGLRFYD DLFDELLKHH IEPVVTISHY EMPLGLVKNY GGWKNRKVIE
160 170 180 190 200
FYERYAKTVF KRYQHKVKYW MTFNEINVVL HAPFTGGGLV FEEGENKLNA
210 220 230 240 250
MYQAAHHQFV ASALAVKAGH DIIPDSKIGC MIAATTTYPM TSKPEDVFAA
260 270 280 290 300
MENERKTLFF SDVQARGAYP GYMKRYLAEN NIEIEMAEGD EELLKEHTVD
310 320 330 340 350
YIGFSYYMSM AASTDPEELA KSGGNLLGGV KNPYLKSSEW GWQIDPKGLR
360 370 380 390 400
ITLNTLYDRY QKPLFIVENG LGAVDKVEED GTIQDDYRIN YLRDHLIEAR
410 420 430 440 450
EAIADGVELI GYTSWGPIDL VSASTAEMKK RYGFIYVDRD NEGNGTFNRI
460
KKKSFNWYQQ VIATNGESL
Length:469
Mass (Da):53,289
Last modified:May 30, 2000 - v2
Checksum:i4C0CA25F964C1CC1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti402 – 4021A → R in CAA84287. (PubMed:7883710)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34526 Genomic DNA. Translation: CAA84287.1.
D31856 Genomic DNA. Translation: BAA06653.1.
D29985 Genomic DNA. Translation: BAA06257.1.
AL009126 Genomic DNA. Translation: CAB15962.2.
PIRiH69593.
RefSeqiNP_391805.2. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB15962; CAB15962; BSU39260.
GeneIDi937512.
KEGGibsu:BSU39260.
PATRICi18979912. VBIBacSub10457_4118.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34526 Genomic DNA. Translation: CAA84287.1 .
D31856 Genomic DNA. Translation: BAA06653.1 .
D29985 Genomic DNA. Translation: BAA06257.1 .
AL009126 Genomic DNA. Translation: CAB15962.2 .
PIRi H69593.
RefSeqi NP_391805.2. NC_000964.3.

3D structure databases

ProteinModelPortali P40740.
SMRi P40740. Positions 1-469.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU39260.

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbi P40740.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB15962 ; CAB15962 ; BSU39260 .
GeneIDi 937512.
KEGGi bsu:BSU39260.
PATRICi 18979912. VBIBacSub10457_4118.

Organism-specific databases

GenoListi BSU39260. [Micado ]

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000088631.
InParanoidi P40740.
KOi K01223.
OMAi NAMYQAA.
OrthoDBi EOG6F81PM.
PhylomeDBi P40740.

Enzyme and pathway databases

BioCyci BSUB:BSU39260-MONOMER.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "New beta-glucoside (bgl) genes in Bacillus subtilis: the bglP gene product has both transport and regulatory functions similar to those of BglF, its Escherichia coli homolog."
    Le Coq D., Lindner C., Krueger S., Steinmetz M., Stuelke J.
    J. Bacteriol. 177:1527-1535(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
  2. "Cloning and sequencing of a 29 kb region of the Bacillus subtilis genome containing the hut and wapA loci."
    Yoshida K., Sano H., Seki S., Oda M., Fujimura M., Fujita Y.
    Microbiology 141:337-343(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / BGSC1A1.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "Identification of aryl-phospho-beta-D-glucosidases in Bacillus subtilis."
    Setlow B., Cabrera-Hernandez A., Cabrera-Martinez R.M., Setlow P.
    Arch. Microbiol. 181:60-67(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN ARYL-PHOSPHO-BETA-D-GLUCOSIDASE, DEVELOPMENTAL STAGE, INDUCTION.
    Strain: 168 / PS832.

Entry informationi

Entry nameiBGLH_BACSU
AccessioniPrimary (citable) accession number: P40740
Secondary accession number(s): O32287
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: May 30, 2000
Last modified: October 29, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3