SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P40740

- BGLH_BACSU

UniProt

P40740 - BGLH_BACSU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Aryl-phospho-beta-D-glucosidase BglH
Gene
bglH, BSU39260, N17D
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of aryl-phospho-beta-D-glucosides such as 4-methylumbelliferyl-phospho-beta-D-glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Plays a major role in the utilization of arbutin or salicin as the sole carbon source. BglA and BglH are the major proteins contributing to hydrolysis of MUG-P by extracts of late-exponential-phase or stationary-phase B.subtilis cells.1 Publication

Catalytic activityi

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei175 – 1751Proton donor Reviewed prediction
Active sitei368 – 3681Nucleophile By similarity

GO - Molecular functioni

  1. 6-phospho-beta-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciBSUB:BSU39260-MONOMER.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Aryl-phospho-beta-D-glucosidase BglH (EC:3.2.1.86)
Alternative name(s):
6-phospho-beta-glucosidase
Gene namesi
Name:bglH
Ordered Locus Names:BSU39260
ORF Names:N17D
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU39260. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Aryl-phospho-beta-D-glucosidase BglH
PRO_0000063873Add
BLAST

Proteomic databases

PaxDbiP40740.

Expressioni

Developmental stagei

Significantly expressed throughout all stages of growth or development.1 Publication

Inductioni

Highly up-regulated by aryl-beta-D-glucosides such as salicin or 4-methylumbelliferyl-beta-D-glucopyranoside (MUG).1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU39260.

Structurei

3D structure databases

ProteinModelPortaliP40740.
SMRiP40740. Positions 1-469.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088631.
KOiK01223.
OMAiNAMYQAA.
OrthoDBiEOG6F81PM.
PhylomeDBiP40740.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40740-1 [UniParc]FASTAAdd to Basket

« Hide

MSSNEKRFPE GFLWGGAVAA NQVEGAYNEG GKGLSTADVS PNGIMSPFDE    50
SMTSLNLYHN GIDFYHRYKE DIALFAEMGF KAFRTSIAWT RIFPNGDEEE 100
PNEEGLRFYD DLFDELLKHH IEPVVTISHY EMPLGLVKNY GGWKNRKVIE 150
FYERYAKTVF KRYQHKVKYW MTFNEINVVL HAPFTGGGLV FEEGENKLNA 200
MYQAAHHQFV ASALAVKAGH DIIPDSKIGC MIAATTTYPM TSKPEDVFAA 250
MENERKTLFF SDVQARGAYP GYMKRYLAEN NIEIEMAEGD EELLKEHTVD 300
YIGFSYYMSM AASTDPEELA KSGGNLLGGV KNPYLKSSEW GWQIDPKGLR 350
ITLNTLYDRY QKPLFIVENG LGAVDKVEED GTIQDDYRIN YLRDHLIEAR 400
EAIADGVELI GYTSWGPIDL VSASTAEMKK RYGFIYVDRD NEGNGTFNRI 450
KKKSFNWYQQ VIATNGESL 469
Length:469
Mass (Da):53,289
Last modified:May 30, 2000 - v2
Checksum:i4C0CA25F964C1CC1
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti402 – 4021A → R in CAA84287. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z34526 Genomic DNA. Translation: CAA84287.1.
D31856 Genomic DNA. Translation: BAA06653.1.
D29985 Genomic DNA. Translation: BAA06257.1.
AL009126 Genomic DNA. Translation: CAB15962.2.
PIRiH69593.
RefSeqiNP_391805.2. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB15962; CAB15962; BSU39260.
GeneIDi937512.
KEGGibsu:BSU39260.
PATRICi18979912. VBIBacSub10457_4118.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z34526 Genomic DNA. Translation: CAA84287.1 .
D31856 Genomic DNA. Translation: BAA06653.1 .
D29985 Genomic DNA. Translation: BAA06257.1 .
AL009126 Genomic DNA. Translation: CAB15962.2 .
PIRi H69593.
RefSeqi NP_391805.2. NC_000964.3.

3D structure databases

ProteinModelPortali P40740.
SMRi P40740. Positions 1-469.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU39260.

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbi P40740.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB15962 ; CAB15962 ; BSU39260 .
GeneIDi 937512.
KEGGi bsu:BSU39260.
PATRICi 18979912. VBIBacSub10457_4118.

Organism-specific databases

GenoListi BSU39260. [Micado ]

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000088631.
KOi K01223.
OMAi NAMYQAA.
OrthoDBi EOG6F81PM.
PhylomeDBi P40740.

Enzyme and pathway databases

BioCyci BSUB:BSU39260-MONOMER.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "New beta-glucoside (bgl) genes in Bacillus subtilis: the bglP gene product has both transport and regulatory functions similar to those of BglF, its Escherichia coli homolog."
    Le Coq D., Lindner C., Krueger S., Steinmetz M., Stuelke J.
    J. Bacteriol. 177:1527-1535(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
  2. "Cloning and sequencing of a 29 kb region of the Bacillus subtilis genome containing the hut and wapA loci."
    Yoshida K., Sano H., Seki S., Oda M., Fujimura M., Fujita Y.
    Microbiology 141:337-343(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / BGSC1A1.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "Identification of aryl-phospho-beta-D-glucosidases in Bacillus subtilis."
    Setlow B., Cabrera-Hernandez A., Cabrera-Martinez R.M., Setlow P.
    Arch. Microbiol. 181:60-67(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN ARYL-PHOSPHO-BETA-D-GLUCOSIDASE, DEVELOPMENTAL STAGE, INDUCTION.
    Strain: 168 / PS832.

Entry informationi

Entry nameiBGLH_BACSU
AccessioniPrimary (citable) accession number: P40740
Secondary accession number(s): O32287
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: May 30, 2000
Last modified: July 9, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi