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P40740

- BGLH_BACSU

UniProt

P40740 - BGLH_BACSU

Protein

Aryl-phospho-beta-D-glucosidase BglH

Gene

bglH

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the hydrolysis of aryl-phospho-beta-D-glucosides such as 4-methylumbelliferyl-phospho-beta-D-glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Plays a major role in the utilization of arbutin or salicin as the sole carbon source. BglA and BglH are the major proteins contributing to hydrolysis of MUG-P by extracts of late-exponential-phase or stationary-phase B.subtilis cells.1 Publication

    Catalytic activityi

    6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei175 – 1751Proton donorSequence Analysis
    Active sitei368 – 3681NucleophilePROSITE-ProRule annotation

    GO - Molecular functioni

    1. 6-phospho-beta-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Enzyme and pathway databases

    BioCyciBSUB:BSU39260-MONOMER.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aryl-phospho-beta-D-glucosidase BglH (EC:3.2.1.86)
    Alternative name(s):
    6-phospho-beta-glucosidase
    Gene namesi
    Name:bglH
    Ordered Locus Names:BSU39260
    ORF Names:N17D
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU39260. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 469469Aryl-phospho-beta-D-glucosidase BglHPRO_0000063873Add
    BLAST

    Proteomic databases

    PaxDbiP40740.

    Expressioni

    Developmental stagei

    Significantly expressed throughout all stages of growth or development.1 Publication

    Inductioni

    Highly up-regulated by aryl-beta-D-glucosides such as salicin or 4-methylumbelliferyl-beta-D-glucopyranoside (MUG).1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU39260.

    Structurei

    3D structure databases

    ProteinModelPortaliP40740.
    SMRiP40740. Positions 1-469.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088631.
    KOiK01223.
    OMAiNAMYQAA.
    OrthoDBiEOG6F81PM.
    PhylomeDBiP40740.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40740-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSNEKRFPE GFLWGGAVAA NQVEGAYNEG GKGLSTADVS PNGIMSPFDE    50
    SMTSLNLYHN GIDFYHRYKE DIALFAEMGF KAFRTSIAWT RIFPNGDEEE 100
    PNEEGLRFYD DLFDELLKHH IEPVVTISHY EMPLGLVKNY GGWKNRKVIE 150
    FYERYAKTVF KRYQHKVKYW MTFNEINVVL HAPFTGGGLV FEEGENKLNA 200
    MYQAAHHQFV ASALAVKAGH DIIPDSKIGC MIAATTTYPM TSKPEDVFAA 250
    MENERKTLFF SDVQARGAYP GYMKRYLAEN NIEIEMAEGD EELLKEHTVD 300
    YIGFSYYMSM AASTDPEELA KSGGNLLGGV KNPYLKSSEW GWQIDPKGLR 350
    ITLNTLYDRY QKPLFIVENG LGAVDKVEED GTIQDDYRIN YLRDHLIEAR 400
    EAIADGVELI GYTSWGPIDL VSASTAEMKK RYGFIYVDRD NEGNGTFNRI 450
    KKKSFNWYQQ VIATNGESL 469
    Length:469
    Mass (Da):53,289
    Last modified:May 30, 2000 - v2
    Checksum:i4C0CA25F964C1CC1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti402 – 4021A → R in CAA84287. (PubMed:7883710)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z34526 Genomic DNA. Translation: CAA84287.1.
    D31856 Genomic DNA. Translation: BAA06653.1.
    D29985 Genomic DNA. Translation: BAA06257.1.
    AL009126 Genomic DNA. Translation: CAB15962.2.
    PIRiH69593.
    RefSeqiNP_391805.2. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB15962; CAB15962; BSU39260.
    GeneIDi937512.
    KEGGibsu:BSU39260.
    PATRICi18979912. VBIBacSub10457_4118.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z34526 Genomic DNA. Translation: CAA84287.1 .
    D31856 Genomic DNA. Translation: BAA06653.1 .
    D29985 Genomic DNA. Translation: BAA06257.1 .
    AL009126 Genomic DNA. Translation: CAB15962.2 .
    PIRi H69593.
    RefSeqi NP_391805.2. NC_000964.3.

    3D structure databases

    ProteinModelPortali P40740.
    SMRi P40740. Positions 1-469.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU39260.

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PaxDbi P40740.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB15962 ; CAB15962 ; BSU39260 .
    GeneIDi 937512.
    KEGGi bsu:BSU39260.
    PATRICi 18979912. VBIBacSub10457_4118.

    Organism-specific databases

    GenoListi BSU39260. [Micado ]

    Phylogenomic databases

    eggNOGi COG2723.
    HOGENOMi HOG000088631.
    KOi K01223.
    OMAi NAMYQAA.
    OrthoDBi EOG6F81PM.
    PhylomeDBi P40740.

    Enzyme and pathway databases

    BioCyci BSUB:BSU39260-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "New beta-glucoside (bgl) genes in Bacillus subtilis: the bglP gene product has both transport and regulatory functions similar to those of BglF, its Escherichia coli homolog."
      Le Coq D., Lindner C., Krueger S., Steinmetz M., Stuelke J.
      J. Bacteriol. 177:1527-1535(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
    2. "Cloning and sequencing of a 29 kb region of the Bacillus subtilis genome containing the hut and wapA loci."
      Yoshida K., Sano H., Seki S., Oda M., Fujimura M., Fujita Y.
      Microbiology 141:337-343(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / BGSC1A1.
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    4. "Identification of aryl-phospho-beta-D-glucosidases in Bacillus subtilis."
      Setlow B., Cabrera-Hernandez A., Cabrera-Martinez R.M., Setlow P.
      Arch. Microbiol. 181:60-67(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS AN ARYL-PHOSPHO-BETA-D-GLUCOSIDASE, DEVELOPMENTAL STAGE, INDUCTION.
      Strain: 168 / PS832.

    Entry informationi

    Entry nameiBGLH_BACSU
    AccessioniPrimary (citable) accession number: P40740
    Secondary accession number(s): O32287
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 103 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3