P40720 (FUMA_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fumarate hydratase class I, aerobic
Short name=Fumarase
EC=4.2.1.2

Gene names

Name:	fumA
Ordered Locus Names:	STM1468

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically By similarity.
Catalytic activity	(S)-malate = fumarate + H2O.
Cofactor	Binds 1 4Fe-4S cluster.
Enzyme regulation	Subject to aerobic respiratory control and catabolite repression By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the class-I fumarase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW fumarate hydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 580	579	Fumarate hydratase class I, aerobic		PRO_0000195659
Sites
	Active site	397	1	Potential
	Metal binding	318	1	Iron-sulfur (4Fe-4S) By similarity
	Binding site	463	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	P40720 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: DF36C9E67D6FBE39	FASTA	580	63,825
	10 20 30 40 50 60 MSNKPFFYQD PFPLKKDDTE YYLLTSEHVS VAEFEGQEIL KVAPEALTLL ARQAFHDASF 70 80 90 100 110 120 MLRPAHQQQV ADILRDPQAS ENDKYVALQF LRNSDIAAKG VLPTCQDTGT AIIVGKKGQR 130 140 150 160 170 180 VWTGGGDEAA LARGVYNTYI EDNLRYSQNA ALDMYKEVNT GTNLPAQIDL YSVDGDEYKF 190 200 210 220 230 240 LCIAKGGGSA NKTYLYQETK ALLTPGKLKN YLVDKMRTLG TAACPPYHIA FVIGGTSAEA 250 260 270 280 290 300 NLKTVKLASA KYYDALPTEG NEHGQAFRDI ELEKELLLEA QNLGLGAQFG GKYFAHDIRV 310 320 330 340 350 360 IRLPRHGASC PVGMGVSCSA DRNIKAKINR DGIWIEKLER NPGKYIPEAL RQAGEGEAVR 370 380 390 400 410 420 VDLNRPMSEI LQQLSQYPVS TRLSLNGTII VGRDIAHAKL KERMDRGEGL PQYIKDHPIY 430 440 450 460 470 480 YAGPAKTPEG YASGSLGPTT AGRMDSYVDQ LQSQGGSMIM LAKGNRSQQV TDACKKHGGF 490 500 510 520 530 540 YLGSIGGPAA VLAQGSIKRL ECVEYPELGM EAIWKIEVED FPAFILVDDK GNDFFQQIQS 550 560 570 580 SQCGAALSNV AALRGGNMIR YFAGERRKRL IRSTPLCCYR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[2]	"Sequence of the phosphomannose isomerase-encoding gene of Salmonella typhimurium." Collins L.V., Hackett J. Gene 103:135-136(1991) [PubMed: 1879695] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-39. Strain: C5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006468 Genomic DNA. Translation: AAL20388.1. X57117 Genomic DNA. No translation available.
RefSeq	NP_460429.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P40720.
ModBase	Search...
Proteomic databases
PRIDE	P40720.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1252986.
GenomeReviews	Gene locus STM1468 in contig AE006468_GR.
KEGG	stm:STM1468.
NMPDR	fig|99287.1.peg.1420.
PATRIC	32381419. VBISalEnt20916_1550.
Phylogenomic databases
HOGENOM	HBG294102.
OMA	IEGEVTM.
ProtClustDB	PRK15390.
Enzyme and pathway databases
BioCyc	STYP99287:STM1468-MONOMER.
Family and domain databases
InterPro	IPR004646. Fe-S_hydro-lyase_TtdA-typ_cat. IPR004647. Fe-S_hydro-lyase_TtdB-typ_cat. IPR011167. Fe_dep_fumarate_hydratase. IPR020557. Fumarate_lyase_CS. [Graphical view]
Gene3D	G3DSA:3.20.130.10. TtdB_fumA_fumB. 1 hit.
KO	K01676.
Pfam	PF05681. Fumerase. 1 hit. PF05683. Fumerase_C. 1 hit. [Graphical view]
PIRSF	PIRSF001394. Fe_dep_fumar_hy. 1 hit.
SUPFAM	SSF117457. SSF117457. 1 hit.
TIGRFAMs	TIGR00722. TtdA_fumA_fumB. 1 hit. TIGR00723. TtdB_fumA_fumB. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMA_SALTY

Accession

Primary (citable) accession number: P40720

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 82 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program