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Protein

Fumarate hydratase class I, aerobic

Gene

fumA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible hydration of fumarate to (S)-malate. Functions as an aerobic enzyme in the direction of malate formation as part of the citric acid cycle. Accounts for about 80% of the fumarase activity when the bacteria grow aerobically. To a lesser extent, also displays D-tartrate dehydratase activity in vitro, but is not able to convert (R)-malate, L-tartrate or meso-tartrate. Can also catalyze the isomerization of enol- to keto-oxaloacetate.By similarity

Catalytic activityi

(S)-malate = fumarate + H2O.By similarity
Keto-oxaloacetate = enol-oxaloacetate.By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class I, aerobic (fumA), Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class I, aerobicBy similarity (EC:4.2.1.2By similarity)
Alternative name(s):
Fumarase ABy similarity
Oxaloacetate keto--enol-isomeraseBy similarity
Short name:
OAAKE isomeraseBy similarity
Oxaloacetate tautomeraseBy similarity (EC:5.3.2.2By similarity)
Gene namesi
Name:fumABy similarity
Ordered Locus Names:STM1468
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001956591 – 580Fumarate hydratase class I, aerobicAdd BLAST580

Proteomic databases

PaxDbiP40720.
PRIDEiP40720.

Expressioni

Inductioni

Is expressed under aerobic conditions. Is repressed by glucose and anaerobiosis.By similarity

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi99287.STM1468.

Structurei

3D structure databases

ProteinModelPortaliP40720.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-I fumarase family.Curated

Phylogenomic databases

eggNOGiENOG4107GFK. Bacteria.
COG1838. LUCA.
COG1951. LUCA.
HOGENOMiHOG000009338.
KOiK01676.
OMAiKNTANNM.
PhylomeDBiP40720.

Family and domain databases

Gene3Di3.20.130.10. 1 hit.
InterProiIPR004646. Fe-S_hydro-lyase_TtdA-typ_cat.
IPR004647. Fe-S_hydro-lyase_TtdB-typ_cat.
IPR011167. Fe_dep_fumarate_hydratase.
IPR020557. Fumarate_lyase_CS.
[Graphical view]
PfamiPF05681. Fumerase. 1 hit.
PF05683. Fumerase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF001394. Fe_dep_fumar_hy. 1 hit.
SUPFAMiSSF117457. SSF117457. 1 hit.
TIGRFAMsiTIGR00722. ttdA_fumA_fumB. 1 hit.
TIGR00723. ttdB_fumA_fumB. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40720-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNKPFFYQD PFPLKKDDTE YYLLTSEHVS VAEFEGQEIL KVAPEALTLL
60 70 80 90 100
ARQAFHDASF MLRPAHQQQV ADILRDPQAS ENDKYVALQF LRNSDIAAKG
110 120 130 140 150
VLPTCQDTGT AIIVGKKGQR VWTGGGDEAA LARGVYNTYI EDNLRYSQNA
160 170 180 190 200
ALDMYKEVNT GTNLPAQIDL YSVDGDEYKF LCIAKGGGSA NKTYLYQETK
210 220 230 240 250
ALLTPGKLKN YLVDKMRTLG TAACPPYHIA FVIGGTSAEA NLKTVKLASA
260 270 280 290 300
KYYDALPTEG NEHGQAFRDI ELEKELLLEA QNLGLGAQFG GKYFAHDIRV
310 320 330 340 350
IRLPRHGASC PVGMGVSCSA DRNIKAKINR DGIWIEKLER NPGKYIPEAL
360 370 380 390 400
RQAGEGEAVR VDLNRPMSEI LQQLSQYPVS TRLSLNGTII VGRDIAHAKL
410 420 430 440 450
KERMDRGEGL PQYIKDHPIY YAGPAKTPEG YASGSLGPTT AGRMDSYVDQ
460 470 480 490 500
LQSQGGSMIM LAKGNRSQQV TDACKKHGGF YLGSIGGPAA VLAQGSIKRL
510 520 530 540 550
ECVEYPELGM EAIWKIEVED FPAFILVDDK GNDFFQQIQS SQCGAALSNV
560 570 580
AALRGGNMIR YFAGERRKRL IRSTPLCCYR
Length:580
Mass (Da):63,825
Last modified:January 23, 2007 - v3
Checksum:iDF36C9E67D6FBE39
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL20388.1.
X57117 Genomic DNA. No translation available.
RefSeqiNP_460429.1. NC_003197.1.
WP_000066602.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20388; AAL20388; STM1468.
GeneIDi1252986.
KEGGistm:STM1468.
PATRICi32381419. VBISalEnt20916_1550.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL20388.1.
X57117 Genomic DNA. No translation available.
RefSeqiNP_460429.1. NC_003197.1.
WP_000066602.1. NC_003197.1.

3D structure databases

ProteinModelPortaliP40720.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM1468.

Proteomic databases

PaxDbiP40720.
PRIDEiP40720.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20388; AAL20388; STM1468.
GeneIDi1252986.
KEGGistm:STM1468.
PATRICi32381419. VBISalEnt20916_1550.

Phylogenomic databases

eggNOGiENOG4107GFK. Bacteria.
COG1838. LUCA.
COG1951. LUCA.
HOGENOMiHOG000009338.
KOiK01676.
OMAiKNTANNM.
PhylomeDBiP40720.

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Family and domain databases

Gene3Di3.20.130.10. 1 hit.
InterProiIPR004646. Fe-S_hydro-lyase_TtdA-typ_cat.
IPR004647. Fe-S_hydro-lyase_TtdB-typ_cat.
IPR011167. Fe_dep_fumarate_hydratase.
IPR020557. Fumarate_lyase_CS.
[Graphical view]
PfamiPF05681. Fumerase. 1 hit.
PF05683. Fumerase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF001394. Fe_dep_fumar_hy. 1 hit.
SUPFAMiSSF117457. SSF117457. 1 hit.
TIGRFAMsiTIGR00722. ttdA_fumA_fumB. 1 hit.
TIGR00723. ttdB_fumA_fumB. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFUMA_SALTY
AccessioniPrimary (citable) accession number: P40720
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.