P40714 (CSCA_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sucrose-6-phosphate hydrolase
Short name=Sucrase
EC=3.2.1.26

Alternative name(s):
Invertase

Gene names

Name:

cscA

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Enables the bacterium to metabolize sucrose as a sole carbon source.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Pathway	Glycan biosynthesis; sucrose metabolism.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	Sucrose-6-phosphate hydrolase		PRO_0000169879
Regions
	Region	36 – 39	4	Substrate binding By similarity
	Region	98 – 99	2	Substrate binding By similarity
	Region	160 – 161	2	Substrate binding By similarity
Sites
	Active site	39	1	By similarity
	Binding site	55	1	Substrate By similarity
	Binding site	63	1	Substrate By similarity
	Binding site	215	1	Substrate By similarity
	Binding site	298	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P40714 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 48E6622AEA380EBD	FASTA	477	54,363
	10 20 30 40 50 60 MTQSRLHAAQ NALAKLHERR GNTFYPHFHL APPAGWMNDP NGLIWFNDRY HAFYQHHPMS 70 80 90 100 110 120 EHWGPMHWGH ATSDDMIHWQ HEPIALAPGD ENDKDGCFSG SAVDDNGVLS LIYTGHVWLD 130 140 150 160 170 180 GAGNDDAIRE VQCLATSRDG IHFEKQGVIL TPPEGIMHFR DPKVWREADT WWMVVGAKDP 190 200 210 220 230 240 GNTGQILLYR GSSLREWTFD RVLAHADAGE SYMWECPDFF SLGDQHYLMF SPQGMNAEGY 250 260 270 280 290 300 SYRNRFQSGV IPGMWSPGRL FAQSGHFTEL DNGHDFYAPQ SFVAKDGRRI VIGWMDMWES 310 320 330 340 350 360 PMPSKREGWA GCMTLARELS ESNGKLLQRP VHEAESLRQQ HQSISPRTIS NKYVLQENAQ 370 380 390 400 410 420 AVEIQLQWAL KNSDAEHYGL QLGAGMRLYI DNQSERLVLW RYYPHENLDG YRSIPLPQGD 430 440 450 460 470 MLALRIFIDT SSVEVFINDG EAVMSSRIYP QPEERELSLY ASHGVAVLQH GALWQLG

References

[1]	"Adaptation of sucrose metabolism in the Escherichia coli wild-type strain EC3132." Jahreis K., Bentler L., Bockmann J., Hans S., Meyer A., Siepelmeyer J., Lengeler J.W. J. Bacteriol. 184:5307-5316(2002) [PubMed: 12218016] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: EC3132.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X81461 Genomic DNA. Translation: CAA57219.1.
PIR	S52162.
3D structure databases
ProteinModelPortal	P40714.
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_43_beta-prop. IPR006232. Suc6P_hydrolase. [Graphical view]
Gene3D	G3DSA:2.115.10.20. Glyco_hydro_43_beta-prop. 1 hit.
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
TIGRFAMs	TIGR01322. ScrB_fam. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CSCA_ECOLX

Accession

Primary (citable) accession number: P40714

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	September 21, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program