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Protein

DNA-binding protein SMUBP-2

Gene

Ighmbp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

5' to 3' helicase that unwinds RNA and DNA duplices in an ATP-dependent reaction. Acts as a transcription regulator. Required for the transcriptional activation of the flounder liver-type antifreeze protein gene. Exhibits strong binding specificity to the enhancer element B of the flounder antifreeze protein gene intron. Binds to the insulin II gene RIPE3B enhancer region (By similarity). May be involved in translation. DNA-binding protein specific to 5'-phosphorylated single-stranded guanine-rich sequence related to the immunoglobulin mu chain switch region. Preferentially binds to the 5'-GGGCT-3' motif. Interacts with tRNA-Tyr.By similarity2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi213 – 2208ATPBy similarity
Zinc fingeri892 – 93847AN1-typeAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • neuromuscular process Source: MGI
  • protein homooligomerization Source: MGI
  • spinal cord motor neuron differentiation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • translation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator, Helicase, Hydrolase, Ribonucleoprotein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein SMUBP-2 (EC:3.6.4.12, EC:3.6.4.13)
Alternative name(s):
ATP-dependent helicase IGHMBP2
Cardiac transcription factor 1
Short name:
CATF1
Immunoglobulin mu-binding protein 2
Gene namesi
Name:Ighmbp2
Synonyms:Smbp-2, Smbp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:99954. Ighmbp2.

Subcellular locationi

GO - Cellular componenti

  • axon Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • growth cone Source: UniProtKB
  • intracellular ribonucleoprotein complex Source: UniProtKB
  • membrane Source: MGI
  • neuronal cell body Source: MGI
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 993992DNA-binding protein SMUBP-2PRO_0000080703Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei797 – 7971PhosphoserineCombined sources
Modified residuei800 – 8001PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP40694.
PaxDbiP40694.
PeptideAtlasiP40694.
PRIDEiP40694.

PTM databases

iPTMnetiP40694.
PhosphoSiteiP40694.

Expressioni

Tissue specificityi

In all tissues examined.

Gene expression databases

CleanExiMM_IGHMBP2.

Interactioni

Subunit structurei

Homooligomer (By similarity). Interacts with RUVBL1, RUVBL2, GTF3C1 and ABT1 (By similarity). Is part of large cytosolic ribonucleoprotein complexes (Probable). Associates with the ribosomes.By similarityCurated1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiP40694. 1 interaction.
MINTiMINT-1676267.
STRINGi10090.ENSMUSP00000025751.

Structurei

3D structure databases

ProteinModelPortaliP40694.
SMRiP40694. Positions 3-647, 725-784.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini721 – 78464R3HPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni637 – 783147SS DNA-bindingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi862 – 8665Nuclear localization signalSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi249 – 425177Leu-richAdd
BLAST
Compositional biasi370 – 3734Poly-Val
Compositional biasi793 – 86169Gln/Pro-richAdd
BLAST
Compositional biasi862 – 8665Poly-Lys

Sequence similaritiesi

Belongs to the DNA2/NAM7 helicase family.Curated
Contains 1 AN1-type zinc finger.Curated
Contains 1 R3H domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri892 – 93847AN1-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1803. Eukaryota.
COG1112. LUCA.
HOGENOMiHOG000185831.
HOVERGENiHBG077019.
InParanoidiP40694.
PhylomeDBiP40694.

Family and domain databases

Gene3Di3.30.1370.50. 1 hit.
3.40.50.300. 3 hits.
4.10.1110.10. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR014001. Helicase_ATP-bd.
IPR033098. IGHMBP2.
IPR027417. P-loop_NTPase.
IPR001374. R3H_dom.
IPR004483. SMUBP-2/Hcs1-like.
IPR000058. Znf_AN1.
[Graphical view]
PANTHERiPTHR10887:SF375. PTHR10887:SF375. 1 hit.
PfamiPF01424. R3H. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00487. DEXDc. 1 hit.
SM00393. R3H. 1 hit.
SM00154. ZnF_AN1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF82708. SSF82708. 1 hit.
TIGRFAMsiTIGR00376. TIGR00376. 1 hit.
PROSITEiPS51061. R3H. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40694-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSTVESFV AQQLQLLELE RDAEVEERRS WQEHSSLREL QSRGVCLLKL
60 70 80 90 100
QVSSQRTGLY GQRLVTFEPR KFGPAVVLPS NSFTSGDIVG LYDTNENSQL
110 120 130 140 150
ATGVLTRITQ KSVTVAFDES HDLQLNLDRE NTYRLLKLAN DVTYKRLKKA
160 170 180 190 200
LMTLKKYHSG PASSLIDILL GSSTPSPAME IPPLSFYNTT LDLSQKEAVS
210 220 230 240 250
FALAQKELAI IHGPPGTGKT TTVVEIILQA VKQGLKVLCC APSNIAVDNL
260 270 280 290 300
VERLALCKKR ILRLGHPARL LESVQHHSLD AVLARSDNAQ IVADIRRDID
310 320 330 340 350
QVFGKNKKTQ DKREKGNFRS EIKLLRKELK EREEAAIVQS LTAADVVLAT
360 370 380 390 400
NTGASSDGPL KLLPEDYFDV VVVDECAQAL EASCWIPLLK APKCILAGDH
410 420 430 440 450
RQLPPTTVSH RAALAGLSRS LMERLAEKHG AGVVRMLTVQ YRMHQAIMCW
460 470 480 490 500
ASEAMYHGQF TSHPSVAGHL LKDLPGVTDT EETRVPLLLI DTAGCGLLEL
510 520 530 540 550
EEEDSQSKGN PGEVRLVTLH IQALVDAGVQ AGDIAVIAPY NLQVDLLRQS
560 570 580 590 600
LSNKHPELEI KSVDGFQGRE KEAVLLTFVR SNRKGEVGFL AEDRRINVAV
610 620 630 640 650
TRARRHVAVI CDSHTVNNHA FLETLVDYFT EHGEVRTAFE YLDDIVPENY
660 670 680 690 700
THEGSQGHSR VPKPKCPSTS IRKPASDQES GQETRAAPRH GRRKPSEKPP
710 720 730 740 750
GSHVQSQHSS SANGSDRTGG PDRTEHFRAT IEEFVASKES QLEFPTSLSS
760 770 780 790 800
HDRLRVHQLA EEFGLRHDST GEGKARHITV SRRSPASSGS VAPQPSSPPS
810 820 830 840 850
PAQAEPEPRA EEPVTVVQAH CPVQLDLKAL HLERLQRQQS SQAQTAKGQP
860 870 880 890 900
GGDSRPQKAS QKKKKKEPKG PVMALPCEED FDALVSAVVK ADNTCSFSKC
910 920 930 940 950
SVSTTTLGQF CMHCSHRYYL SHHLPEIHGC GEKARAHARQ RISREGVLYA
960 970 980 990
GSGTKDRALD PAKRAQLQRR LDKKLGELSS QRTSRKKEKE RGT
Length:993
Mass (Da):109,466
Last modified:February 1, 1995 - v1
Checksum:i2FA0850DBABDE35B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10075 mRNA. Translation: AAA40143.1.
CCDSiCCDS29393.1.
PIRiS35633.
UniGeneiMm.3179.

Genome annotation databases

UCSCiuc008fwa.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10075 mRNA. Translation: AAA40143.1.
CCDSiCCDS29393.1.
PIRiS35633.
UniGeneiMm.3179.

3D structure databases

ProteinModelPortaliP40694.
SMRiP40694. Positions 3-647, 725-784.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP40694. 1 interaction.
MINTiMINT-1676267.
STRINGi10090.ENSMUSP00000025751.

PTM databases

iPTMnetiP40694.
PhosphoSiteiP40694.

Proteomic databases

EPDiP40694.
PaxDbiP40694.
PeptideAtlasiP40694.
PRIDEiP40694.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiuc008fwa.1. mouse.

Organism-specific databases

MGIiMGI:99954. Ighmbp2.

Phylogenomic databases

eggNOGiKOG1803. Eukaryota.
COG1112. LUCA.
HOGENOMiHOG000185831.
HOVERGENiHBG077019.
InParanoidiP40694.
PhylomeDBiP40694.

Miscellaneous databases

PROiP40694.
SOURCEiSearch...

Gene expression databases

CleanExiMM_IGHMBP2.

Family and domain databases

Gene3Di3.30.1370.50. 1 hit.
3.40.50.300. 3 hits.
4.10.1110.10. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR014001. Helicase_ATP-bd.
IPR033098. IGHMBP2.
IPR027417. P-loop_NTPase.
IPR001374. R3H_dom.
IPR004483. SMUBP-2/Hcs1-like.
IPR000058. Znf_AN1.
[Graphical view]
PANTHERiPTHR10887:SF375. PTHR10887:SF375. 1 hit.
PfamiPF01424. R3H. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00487. DEXDc. 1 hit.
SM00393. R3H. 1 hit.
SM00154. ZnF_AN1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF82708. SSF82708. 1 hit.
TIGRFAMsiTIGR00376. TIGR00376. 1 hit.
PROSITEiPS51061. R3H. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSMBP2_MOUSE
AccessioniPrimary (citable) accession number: P40694
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.