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P40676

- SLYA_SALTY

UniProt

P40676 - SLYA_SALTY

Protein

Transcriptional regulator SlyA

Gene

slyA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 3 (24 Oct 2003)
      Previous versions | rss
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    Functioni

    Transcription regulator that can specifically activate or repress expression of target genes. Required for virulence and survival in the macrophage environment. Probably activates expression of ispA, xseB genes, and of omp operon.2 PublicationsUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi49 – 7224H-T-H motifUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-HAMAP
    2. sequence-specific DNA binding transcription factor activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. pathogenesis Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation, Virulence

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciSENT99287:GCTI-1454-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional regulator SlyAUniRule annotation
    Alternative name(s):
    Cytolysin SlyA
    Salmolysin
    Gene namesi
    Name:slyAUniRule annotation
    Ordered Locus Names:STM1444
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 144144Transcriptional regulator SlyAPRO_0000054393Add
    BLAST

    Proteomic databases

    PRIDEiP40676.

    Expressioni

    Inductioni

    During infection of the host cells. Down-regulated by itself. Activated by Mg2+ starvation in a phoP dependent manner.2 Publications

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    DIPiDIP-48666N.
    STRINGi99287.STM1444.

    Structurei

    Secondary structure

    1
    144
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 2319
    Turni24 – 285
    Helixi31 – 4212
    Beta strandi45 – 484
    Helixi49 – 568
    Helixi60 – 7213
    Beta strandi75 – 784
    Beta strandi82 – 843
    Beta strandi89 – 924
    Helixi94 – 963
    Helixi97 – 11519
    Helixi120 – 13920

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3DEUX-ray2.30A/B2-144[»]
    3Q5FX-ray2.96A/B1-144[»]
    3QPTX-ray2.40A1-144[»]
    ProteinModelPortaliP40676.
    SMRiP40676. Positions 1-140.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP40676.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 135134HTH marR-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SlyA family.UniRule annotation
    Contains 1 HTH marR-type DNA-binding domain.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000221454.
    KOiK06075.
    OMAiTRHICAH.
    OrthoDBiEOG6JMMVK.
    PhylomeDBiP40676.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    HAMAPiMF_01819. HTH_type_SlyA.
    InterProiIPR000835. HTH_MarR-typ.
    IPR023187. Tscrpt_reg_MarR-type_CS.
    IPR023071. Tscrpt_reg_SlyA.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF01047. MarR. 1 hit.
    [Graphical view]
    PRINTSiPR00598. HTHMARR.
    SMARTiSM00347. HTH_MARR. 1 hit.
    [Graphical view]
    PROSITEiPS01117. HTH_MARR_1. 1 hit.
    PS50995. HTH_MARR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40676-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESPLGSDLA RLVRIWRALI DHRLKPLELT QTHWVTLHNI HQLPPDQSQI    50
    QLAKAIGIEQ PSLVRTLDQL EDKGLISRQT CASDRRAKRI KLTEKADALI 100
    AEMEEVIHKT RGEILAGISS EEIELLIKLI AKLEHNIMEL HSHD 144
    Length:144
    Mass (Da):16,422
    Last modified:October 24, 2003 - v3
    Checksum:i2D37EB004F2D7439
    GO

    Sequence cautioni

    The sequence AAA58796.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
    The sequence AAL20366.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti97 – 982DA → EP in AAA58796. (PubMed:8290552)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U03842 Genomic DNA. Translation: AAA58796.1. Different initiation.
    AE006468 Genomic DNA. Translation: AAL20366.1. Different initiation.
    PIRiA36874.
    RefSeqiNP_460407.2. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL20366; AAL20366; STM1444.
    GeneIDi1252962.
    KEGGistm:STM1444.
    PATRICi32381373. VBISalEnt20916_1527.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U03842 Genomic DNA. Translation: AAA58796.1 . Different initiation.
    AE006468 Genomic DNA. Translation: AAL20366.1 . Different initiation.
    PIRi A36874.
    RefSeqi NP_460407.2. NC_003197.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3DEU X-ray 2.30 A/B 2-144 [» ]
    3Q5F X-ray 2.96 A/B 1-144 [» ]
    3QPT X-ray 2.40 A 1-144 [» ]
    ProteinModelPortali P40676.
    SMRi P40676. Positions 1-140.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48666N.
    STRINGi 99287.STM1444.

    Proteomic databases

    PRIDEi P40676.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL20366 ; AAL20366 ; STM1444 .
    GeneIDi 1252962.
    KEGGi stm:STM1444.
    PATRICi 32381373. VBISalEnt20916_1527.

    Phylogenomic databases

    HOGENOMi HOG000221454.
    KOi K06075.
    OMAi TRHICAH.
    OrthoDBi EOG6JMMVK.
    PhylomeDBi P40676.

    Enzyme and pathway databases

    BioCyci SENT99287:GCTI-1454-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P40676.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    HAMAPi MF_01819. HTH_type_SlyA.
    InterProi IPR000835. HTH_MarR-typ.
    IPR023187. Tscrpt_reg_MarR-type_CS.
    IPR023071. Tscrpt_reg_SlyA.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF01047. MarR. 1 hit.
    [Graphical view ]
    PRINTSi PR00598. HTHMARR.
    SMARTi SM00347. HTH_MARR. 1 hit.
    [Graphical view ]
    PROSITEi PS01117. HTH_MARR_1. 1 hit.
    PS50995. HTH_MARR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 14028s / SGSG 2262.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.
    3. "SlyA, a regulatory protein from Salmonella typhimurium, induces a haemolytic and pore-forming protein in Escherichia coli."
      Ludwig A., Tengel C., Bauer S., Bubert A., Benz R., Mollenkopf H.-J., Goebel W.
      Mol. Gen. Genet. 249:474-486(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Homologies between salmolysin and some bacterial regulatory proteins."
      Dehoux P., Cossart P.
      Mol. Microbiol. 15:591-591(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: SIMILARITY TO MARR FAMILY.
    5. "SlyA, a transcriptional regulator of Salmonella typhimurium, is required for resistance to oxidative stress and is expressed in the intracellular environment of macrophages."
      Buchmeier N., Bossie S., Chen C.-Y., Fang F.C., Guiney D.G., Libby S.J.
      Infect. Immun. 65:3725-3730(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.
    6. "Interaction of the Salmonella typhimurium transcription and virulence factor SlyA with target DNA and identification of members of the SlyA regulon."
      Stapleton M.R., Norte V.A., Read R.C., Green J.
      J. Biol. Chem. 277:17630-17637(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: HOMODIMERIZATION.
    7. "PhoP-responsive expression of the Salmonella enterica serovar typhimurium slyA gene."
      Norte V.A., Stapleton M.R., Green J.
      J. Bacteriol. 185:3508-3514(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiSLYA_SALTY
    AccessioniPrimary (citable) accession number: P40676
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: October 24, 2003
    Last modified: October 1, 2014
    This is version 106 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally thought to be a toxin with hemolytic and cytolytic activity.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3