SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P40676

- SLYA_SALTY

UniProt

P40676 - SLYA_SALTY

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Transcriptional regulator SlyA

Gene
slyA, STM1444
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Transcription regulator that can specifically activate or repress expression of target genes. Required for virulence and survival in the macrophage environment. Probably activates expression of ispA, xseB genes, and of omp operon.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi49 – 7224H-T-H motif By similarityAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-HAMAP
  2. sequence-specific DNA binding transcription factor activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. pathogenesis Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation, Virulence

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciSENT99287:GCTI-1454-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulator SlyA
Alternative name(s):
Cytolysin SlyA
Salmolysin
Gene namesi
Name:slyA
Ordered Locus Names:STM1444
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 144144Transcriptional regulator SlyAUniRule annotationPRO_0000054393Add
BLAST

Proteomic databases

PRIDEiP40676.

Expressioni

Inductioni

During infection of the host cells. Down-regulated by itself. Activated by Mg2+ starvation in a phoP dependent manner.2 Publications

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

DIPiDIP-48666N.
STRINGi99287.STM1444.

Structurei

Secondary structure

1
144
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 2319
Turni24 – 285
Helixi31 – 4212
Beta strandi45 – 484
Helixi49 – 568
Helixi60 – 7213
Beta strandi75 – 784
Beta strandi82 – 843
Beta strandi89 – 924
Helixi94 – 963
Helixi97 – 11519
Helixi120 – 13920

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DEUX-ray2.30A/B2-144[»]
3Q5FX-ray2.96A/B1-144[»]
3QPTX-ray2.40A1-144[»]
ProteinModelPortaliP40676.
SMRiP40676. Positions 1-140.

Miscellaneous databases

EvolutionaryTraceiP40676.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 135134HTH marR-typeAdd
BLAST

Sequence similaritiesi

Belongs to the SlyA family.

Phylogenomic databases

HOGENOMiHOG000221454.
KOiK06075.
OMAiTRHICAH.
OrthoDBiEOG6JMMVK.
PhylomeDBiP40676.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_01819. HTH_type_SlyA.
InterProiIPR000835. HTH_MarR-typ.
IPR023187. Tscrpt_reg_MarR-type_CS.
IPR023071. Tscrpt_reg_SlyA.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01047. MarR. 1 hit.
[Graphical view]
PRINTSiPR00598. HTHMARR.
SMARTiSM00347. HTH_MARR. 1 hit.
[Graphical view]
PROSITEiPS01117. HTH_MARR_1. 1 hit.
PS50995. HTH_MARR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40676-1 [UniParc]FASTAAdd to Basket

« Hide

MESPLGSDLA RLVRIWRALI DHRLKPLELT QTHWVTLHNI HQLPPDQSQI    50
QLAKAIGIEQ PSLVRTLDQL EDKGLISRQT CASDRRAKRI KLTEKADALI 100
AEMEEVIHKT RGEILAGISS EEIELLIKLI AKLEHNIMEL HSHD 144
Length:144
Mass (Da):16,422
Last modified:October 24, 2003 - v3
Checksum:i2D37EB004F2D7439
GO

Sequence cautioni

The sequence AAA58796.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence AAL20366.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti97 – 982DA → EP in AAA58796. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U03842 Genomic DNA. Translation: AAA58796.1. Different initiation.
AE006468 Genomic DNA. Translation: AAL20366.1. Different initiation.
PIRiA36874.
RefSeqiNP_460407.2. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20366; AAL20366; STM1444.
GeneIDi1252962.
KEGGistm:STM1444.
PATRICi32381373. VBISalEnt20916_1527.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U03842 Genomic DNA. Translation: AAA58796.1 . Different initiation.
AE006468 Genomic DNA. Translation: AAL20366.1 . Different initiation.
PIRi A36874.
RefSeqi NP_460407.2. NC_003197.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3DEU X-ray 2.30 A/B 2-144 [» ]
3Q5F X-ray 2.96 A/B 1-144 [» ]
3QPT X-ray 2.40 A 1-144 [» ]
ProteinModelPortali P40676.
SMRi P40676. Positions 1-140.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48666N.
STRINGi 99287.STM1444.

Proteomic databases

PRIDEi P40676.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL20366 ; AAL20366 ; STM1444 .
GeneIDi 1252962.
KEGGi stm:STM1444.
PATRICi 32381373. VBISalEnt20916_1527.

Phylogenomic databases

HOGENOMi HOG000221454.
KOi K06075.
OMAi TRHICAH.
OrthoDBi EOG6JMMVK.
PhylomeDBi P40676.

Enzyme and pathway databases

BioCyci SENT99287:GCTI-1454-MONOMER.

Miscellaneous databases

EvolutionaryTracei P40676.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
HAMAPi MF_01819. HTH_type_SlyA.
InterProi IPR000835. HTH_MarR-typ.
IPR023187. Tscrpt_reg_MarR-type_CS.
IPR023071. Tscrpt_reg_SlyA.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF01047. MarR. 1 hit.
[Graphical view ]
PRINTSi PR00598. HTHMARR.
SMARTi SM00347. HTH_MARR. 1 hit.
[Graphical view ]
PROSITEi PS01117. HTH_MARR_1. 1 hit.
PS50995. HTH_MARR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 14028s / SGSG 2262.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  3. "SlyA, a regulatory protein from Salmonella typhimurium, induces a haemolytic and pore-forming protein in Escherichia coli."
    Ludwig A., Tengel C., Bauer S., Bubert A., Benz R., Mollenkopf H.-J., Goebel W.
    Mol. Gen. Genet. 249:474-486(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Homologies between salmolysin and some bacterial regulatory proteins."
    Dehoux P., Cossart P.
    Mol. Microbiol. 15:591-591(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: SIMILARITY TO MARR FAMILY.
  5. "SlyA, a transcriptional regulator of Salmonella typhimurium, is required for resistance to oxidative stress and is expressed in the intracellular environment of macrophages."
    Buchmeier N., Bossie S., Chen C.-Y., Fang F.C., Guiney D.G., Libby S.J.
    Infect. Immun. 65:3725-3730(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  6. "Interaction of the Salmonella typhimurium transcription and virulence factor SlyA with target DNA and identification of members of the SlyA regulon."
    Stapleton M.R., Norte V.A., Read R.C., Green J.
    J. Biol. Chem. 277:17630-17637(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: HOMODIMERIZATION.
  7. "PhoP-responsive expression of the Salmonella enterica serovar typhimurium slyA gene."
    Norte V.A., Stapleton M.R., Green J.
    J. Bacteriol. 185:3508-3514(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiSLYA_SALTY
AccessioniPrimary (citable) accession number: P40676
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 24, 2003
Last modified: July 9, 2014
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally (1 Publication) thought to be a toxin with hemolytic and cytolytic activity.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi