Transcriptional regulator SlyA - Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

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P40676 (SLYA_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Transcriptional regulator SlyA

Alternative name(s):
Cytolysin SlyA
Salmolysin

Gene names

Name:	slyA
Ordered Locus Names:	STM1444

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella ›

Protein attributes

Sequence length	144 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription regulator that can specifically activate or repress expression of target genes. Required for virulence and survival in the macrophage environment. Probably activates expression of ispA, xseB genes, and of omp operon. Ref.3 Ref.5
Subunit structure	Homodimer. Ref.6
Induction	During infection of the host cells. Down-regulated by itself. Activated by Mg²⁺ starvation in a phoP dependent manner. Ref.5 Ref.7
Sequence similarities	Belongs to the SlyA family. Contains 1 HTH marR-type DNA-binding domain.
Caution	Was originally (Ref.1) thought to be a toxin with hemolytic and cytolytic activity.
Sequence caution	The sequence AAA58796.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAL20366.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Virulence
Ligand	DNA-binding
Molecular function	Activator Repressor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: HAMAP sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 144

144

Transcriptional regulator SlyA HAMAP-Rule MF_01819

PRO_0000054393

Regions

Domain

2 – 135

134

HTH marR-type

DNA binding

49 – 72

H-T-H motif By similarity

Experimental info

Sequence conflict

97 – 98

DA → EP in AAA58796. Ref.1

Secondary structure

144

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P40676 [UniParc].

Last modified October 24, 2003. Version 3.
Checksum: 2D37EB004F2D7439
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FASTA

144

16,422

        10         20         30         40         50         60 
MESPLGSDLA RLVRIWRALI DHRLKPLELT QTHWVTLHNI HQLPPDQSQI QLAKAIGIEQ 

        70         80         90        100        110        120 
PSLVRTLDQL EDKGLISRQT CASDRRAKRI KLTEKADALI AEMEEVIHKT RGEILAGISS 

       130        140 
EEIELLIKLI AKLEHNIMEL HSHD

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A cytolysin encoded by Salmonella is required for survival within macrophages." Libby S.J., Goebel W., Ludwig A., Buchmeier N., Bowe F., Fang F.C., Guiney D.G., Songer J.G., Heffron F. Proc. Natl. Acad. Sci. U.S.A. 91:489-493(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 14028s / SGSG 2262.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[3]	"SlyA, a regulatory protein from Salmonella typhimurium, induces a haemolytic and pore-forming protein in Escherichia coli." Ludwig A., Tengel C., Bauer S., Bubert A., Benz R., Mollenkopf H.-J., Goebel W. Mol. Gen. Genet. 249:474-486(1995) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Homologies between salmolysin and some bacterial regulatory proteins." Dehoux P., Cossart P. Mol. Microbiol. 15:591-591(1995) [PubMed] [Europe PMC] [Abstract] Cited for: SIMILARITY TO MARR FAMILY.
[5]	"SlyA, a transcriptional regulator of Salmonella typhimurium, is required for resistance to oxidative stress and is expressed in the intracellular environment of macrophages." Buchmeier N., Bossie S., Chen C.-Y., Fang F.C., Guiney D.G., Libby S.J. Infect. Immun. 65:3725-3730(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
[6]	"Interaction of the Salmonella typhimurium transcription and virulence factor SlyA with target DNA and identification of members of the SlyA regulon." Stapleton M.R., Norte V.A., Read R.C., Green J. J. Biol. Chem. 277:17630-17637(2002) [PubMed] [Europe PMC] [Abstract] Cited for: HOMODIMERIZATION.
[7]	"PhoP-responsive expression of the Salmonella enterica serovar typhimurium slyA gene." Norte V.A., Stapleton M.R., Green J. J. Bacteriol. 185:3508-3514(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U03842 Genomic DNA. Translation: AAA58796.1. Different initiation.
AE006468 Genomic DNA. Translation: AAL20366.1. Different initiation.

PIR

A36874.

RefSeq

NP_460407.2. NC_003197.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3DEU	X-ray	2.30	A/B	2-144	[»]
3Q5F	X-ray	2.96	A/B	1-144	[»]
3QPT	X-ray	2.40	A	1-144	[»]

ProteinModelPortal

P40676.

SMR

P40676. Positions 1-140.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48666N.

STRING

99287.STM1444.

Proteomic databases

PRIDE

P40676.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAL20366; AAL20366; STM1444.

GeneID

1252962.

KEGG

stm:STM1444.

PATRIC

32381373. VBISalEnt20916_1527.

Phylogenomic databases

HOGENOM

HOG000221454.

K06075.

OMA

CMRVHAK.

ProtClustDB

PRK03573.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.

HAMAP

MF_01819. HTH-type_SlyA.

InterPro

IPR000835. Tscrpt_reg_HTH_MarR-typ.
IPR023187. Tscrpt_reg_MarR-type_CS.
IPR023071. Tscrpt_reg_SlyA.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF01047. MarR. 1 hit.
[Graphical view]

PRINTS

PR00598. HTHMARR.

SMART

SM00347. HTH_MARR. 1 hit.
[Graphical view]

PROSITE

PS01117. HTH_MARR_1. 1 hit.
PS50995. HTH_MARR_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P40676.

Entry information

Entry name

SLYA_SALTY

Accession

Primary (citable) accession number: P40676

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 24, 2003
Last modified:	May 1, 2013
This is version 96 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents