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P40676 (SLYA_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional regulator SlyA
Alternative name(s):
Cytolysin SlyA
Salmolysin
Gene names
Name:slyA
Ordered Locus Names:STM1444
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length144 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription regulator that can specifically activate or repress expression of target genes. Required for virulence and survival in the macrophage environment. Probably activates expression of ispA, xseB genes, and of omp operon. Ref.3 Ref.5

Subunit structure

Homodimer. Ref.6

Induction

During infection of the host cells. Down-regulated by itself. Activated by Mg2+ starvation in a phoP dependent manner. Ref.5 Ref.7

Sequence similarities

Belongs to the SlyA family.

Contains 1 HTH marR-type DNA-binding domain.

Caution

Was originally (Ref.1) thought to be a toxin with hemolytic and cytolytic activity.

Sequence caution

The sequence AAA58796.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence AAL20366.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 144144Transcriptional regulator SlyA HAMAP-Rule MF_01819
PRO_0000054393

Regions

Domain2 – 135134HTH marR-type
DNA binding49 – 7224H-T-H motif By similarity

Experimental info

Sequence conflict97 – 982DA → EP in AAA58796. Ref.1

Secondary structure

...................... 144
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P40676 [UniParc].

Last modified October 24, 2003. Version 3.
Checksum: 2D37EB004F2D7439

FASTA14416,422
        10         20         30         40         50         60 
MESPLGSDLA RLVRIWRALI DHRLKPLELT QTHWVTLHNI HQLPPDQSQI QLAKAIGIEQ 

        70         80         90        100        110        120 
PSLVRTLDQL EDKGLISRQT CASDRRAKRI KLTEKADALI AEMEEVIHKT RGEILAGISS 

       130        140 
EEIELLIKLI AKLEHNIMEL HSHD 

« Hide

References

« Hide 'large scale' references
[1]"A cytolysin encoded by Salmonella is required for survival within macrophages."
Libby S.J., Goebel W., Ludwig A., Buchmeier N., Bowe F., Fang F.C., Guiney D.G., Songer J.G., Heffron F.
Proc. Natl. Acad. Sci. U.S.A. 91:489-493(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 14028s / SGSG 2262.
[2]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.
[3]"SlyA, a regulatory protein from Salmonella typhimurium, induces a haemolytic and pore-forming protein in Escherichia coli."
Ludwig A., Tengel C., Bauer S., Bubert A., Benz R., Mollenkopf H.-J., Goebel W.
Mol. Gen. Genet. 249:474-486(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Homologies between salmolysin and some bacterial regulatory proteins."
Dehoux P., Cossart P.
Mol. Microbiol. 15:591-591(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: SIMILARITY TO MARR FAMILY.
[5]"SlyA, a transcriptional regulator of Salmonella typhimurium, is required for resistance to oxidative stress and is expressed in the intracellular environment of macrophages."
Buchmeier N., Bossie S., Chen C.-Y., Fang F.C., Guiney D.G., Libby S.J.
Infect. Immun. 65:3725-3730(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
[6]"Interaction of the Salmonella typhimurium transcription and virulence factor SlyA with target DNA and identification of members of the SlyA regulon."
Stapleton M.R., Norte V.A., Read R.C., Green J.
J. Biol. Chem. 277:17630-17637(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: HOMODIMERIZATION.
[7]"PhoP-responsive expression of the Salmonella enterica serovar typhimurium slyA gene."
Norte V.A., Stapleton M.R., Green J.
J. Bacteriol. 185:3508-3514(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U03842 Genomic DNA. Translation: AAA58796.1. Different initiation.
AE006468 Genomic DNA. Translation: AAL20366.1. Different initiation.
PIRA36874.
RefSeqNP_460407.2. NC_003197.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3DEUX-ray2.30A/B2-144[»]
3Q5FX-ray2.96A/B1-144[»]
3QPTX-ray2.40A1-144[»]
ProteinModelPortalP40676.
SMRP40676. Positions 1-140.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-48666N.
STRING99287.STM1444.

Proteomic databases

PRIDEP40676.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL20366; AAL20366; STM1444.
GeneID1252962.
KEGGstm:STM1444.
PATRIC32381373. VBISalEnt20916_1527.

Phylogenomic databases

HOGENOMHOG000221454.
KOK06075.
OMATRHICAH.
OrthoDBEOG6JMMVK.
PhylomeDBP40676.

Enzyme and pathway databases

BioCycSENT99287:GCTI-1454-MONOMER.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
HAMAPMF_01819. HTH_type_SlyA.
InterProIPR000835. HTH_MarR-typ.
IPR023187. Tscrpt_reg_MarR-type_CS.
IPR023071. Tscrpt_reg_SlyA.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF01047. MarR. 1 hit.
[Graphical view]
PRINTSPR00598. HTHMARR.
SMARTSM00347. HTH_MARR. 1 hit.
[Graphical view]
PROSITEPS01117. HTH_MARR_1. 1 hit.
PS50995. HTH_MARR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP40676.

Entry information

Entry nameSLYA_SALTY
AccessionPrimary (citable) accession number: P40676
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 24, 2003
Last modified: July 9, 2014
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references