Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P40645 (SOX6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor SOX-6
Alternative name(s):
SOX-LZ
Gene names
Name:Sox6
Synonyms:Sox-6
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length827 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation.

Subunit structure

Interacts with DAZAP2. May interact with CENPK. Ref.6 Ref.7

Subcellular location

Nucleus By similarity.

Tissue specificity

Highly expressed in testis.

Post-translational modification

Sumoylation inhibits the transcriptional activity By similarity.

Sequence similarities

Contains 1 HMG box DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   LigandDNA-binding
   Molecular functionActivator
Developmental protein
   PTMIsopeptide bond
Ubl conjugation
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processastrocyte differentiation

Inferred from electronic annotation. Source: Ensembl

cardiac muscle cell differentiation

Inferred from direct assay Ref.7. Source: MGI

cartilage development

Inferred from genetic interaction PubMed 15634692. Source: MGI

cell fate commitment

Inferred from genetic interaction PubMed 15634692. Source: MGI

cell morphogenesis

Inferred from mutant phenotype PubMed 16462943. Source: MGI

cellular response to transforming growth factor beta stimulus

Inferred from electronic annotation. Source: Ensembl

erythrocyte development

Inferred from mutant phenotype PubMed 16462943. Source: MGI

erythrocyte differentiation

Inferred from mutant phenotype PubMed 20711497. Source: MGI

gene silencing

Inferred from mutant phenotype PubMed 16462943. Source: MGI

hemopoiesis

Inferred from mutant phenotype PubMed 16462943. Source: MGI

in utero embryonic development

Inferred from mutant phenotype PubMed 17084361. Source: MGI

muscle cell differentiation

Inferred from direct assay Ref.7. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 16462943. Source: MGI

negative regulation of transcription, DNA-templated

Inferred from direct assay PubMed 17084361. Source: MGI

oligodendrocyte cell fate specification

Inferred from mutant phenotype PubMed 17084361. Source: MGI

oligodendrocyte differentiation

Inferred from mutant phenotype PubMed 17084361. Source: MGI

positive regulation of cartilage development

Inferred from electronic annotation. Source: Ensembl

positive regulation of chondrocyte differentiation

Inferred from direct assay PubMed 20940257. Source: UniProtKB

positive regulation of mesenchymal stem cell differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction Ref.3. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 17084361. Source: MGI

post-embryonic development

Inferred from mutant phenotype PubMed 16462943. Source: MGI

regulation of gene expression

Inferred from mutant phenotype PubMed 20711497. Source: MGI

regulation of transcription, DNA-templated

Inferred from direct assay PubMed 12446692. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from direct assay PubMed 21884692Ref.3. Source: MGI

transcription factor complex

Inferred by curator Ref.7. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 12446692. Source: MGI

protein heterodimerization activity

Inferred from physical interaction Ref.3. Source: MGI

sequence-specific DNA binding

Inferred from direct assay PubMed 16462943. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 12446692PubMed 17084361. Source: MGI

transcription regulatory region DNA binding

Inferred from direct assay PubMed 17084361. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: P40645-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P40645-2)

The sequence of this isoform differs from the canonical sequence as follows:
     327-367: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 827827Transcription factor SOX-6
PRO_0000048730

Regions

DNA binding620 – 68869HMG box
Coiled coil184 – 26279 Potential
Compositional bias233 – 26129Gln-rich
Compositional bias240 – 2434Poly-Gln
Compositional bias280 – 2856Poly-Ala
Compositional bias313 – 3175Poly-Ala
Compositional bias514 – 5174Poly-Gln

Amino acid modifications

Cross-link404Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-link417Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Natural variations

Alternative sequence327 – 36741Missing in isoform 2.
VSP_002198

Experimental info

Sequence conflict312 – 3132MA → SS in BAA09618. Ref.2
Sequence conflict6321K → R in CAA46610. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: F777BAB7CFB1E93B

FASTA82791,803
        10         20         30         40         50         60 
MSSKQATSPF ACTADGEEAM TQDLTSREKE EGSDQHPASH LPLHPIMHNK PHSEELPTLV 

        70         80         90        100        110        120 
STIQQDADWD SVLSSQQRME SENNKLCSLY SFRNTSTSPH KPDEGSRERE IMNSVTFGTP 

       130        140        150        160        170        180 
ERRKGSLADV VDTLKQKKLE EMTRTEQEDS SCMEKLLSKD WKEKMERLNT SELLGEIKGT 

       190        200        210        220        230        240 
PESLAEKERQ LSTMITQLIS LREQLLAAHD EQKKLAASQI EKQRQQMDLA RQQQEQIARQ 

       250        260        270        280        290        300 
QQQLLQQQHK INLLQQQIQV QGHMPPLMIP IFPHDQRTLA AAAAAQQGFL FPPGITYKPG 

       310        320        330        340        350        360 
DNYPVQFIPS TMAAAAASGL SPLQLQKGHV SHPQINPRLK GISDRFGRNL DPSEHGGGHS 

       370        380        390        400        410        420 
YNHRQIEQLY AAQLASMQVS PGAKMPSTPQ PPNSAGAVSP TGIKNEKRGT SPVTQVKDET 

       430        440        450        460        470        480 
TAQPLNLSSR PKTAEPVKSP TSPTQNLFPA SKTSPVNLPN KSSIPSPIGG SLGRGSSLDI 

       490        500        510        520        530        540 
LSSLNSPALF GDQDTVMKAI QEARKMREQI QREQQQQPHG VDGKLSSMNN MGLSNCRTEK 

       550        560        570        580        590        600 
ERTRFENLGP QLTGKSSEDG KLGPGVIDLT RPEDAEGSKA MNGSAAKLQQ YYCWPTGGAT 

       610        620        630        640        650        660 
VAEARVYRDA RGRASSEPHI KRPMNAFMVW AKDERRKILQ AFPDMHNSNI SKILGSRWKS 

       670        680        690        700        710        720 
MSNQEKQPYY EEQARLSKIH LEKYPNYKYK PRPKRTCIVD GKKLRIGEYK QLMRSRRQEM 

       730        740        750        760        770        780 
RQFFTVGQQP QMPITTGTGV VYPGAITMAT TTPSPQMTSD CSSTSASPEP SLPVIQSTYG 

       790        800        810        820 
MKMDGASLAG NDMINGEDEM EAYDDYEDDP KSDYSSENEA PEPVSAN 

« Hide

Isoform 2 [UniParc].

Checksum: EFC8E350AEC84653
Show »

FASTA78687,192

References

« Hide 'large scale' references
[1]"The Sry-related HMG box-containing gene Sox6 is expressed in the adult testis and developing nervous system of the mouse."
Connor F., Wright E., Denny P., Koopman P., Ashworth A.
Nucleic Acids Res. 23:3365-3372(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Testis.
[2]"A gene that is related to SRY and is expressed in the testes encodes a leucine zipper-containing protein."
Takamatsu N., Kanda H., Tsuchiya I., Yamada S., Ito M., Kabeno S., Shiba T., Yamashita S.
Mol. Cell. Biol. 15:3759-3766(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: C57BL/6.
Tissue: Testis.
[3]"A new long form of Sox5 (L-Sox5), Sox6 and Sox9 are coexpressed in chondrogenesis and cooperatively activate the type II collagen gene."
Lefebvre V., Li P., de Crombrugghe B.
EMBO J. 17:5718-5733(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 519-827.
[4]"A conserved family of genes related to the testis determining gene, SRY."
Denny P., Swift S., Brand N., Dabhade N., Barton P., Ashworth A.
Nucleic Acids Res. 20:2887-2887(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 631-684.
Strain: Parkes.
Tissue: Brain and Testis.
[5]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 660-666, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[6]"Characterization of Solt, a novel SoxLZ/Sox6 binding protein expressed in adult mouse testis."
Yamashita A., Ito M., Takamatsu N., Shiba T.
FEBS Lett. 481:147-151(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: POSSIBLE INTERACTION WITH CENPK.
[7]"Sox6 regulation of cardiac myocyte development."
Cohen-Barak O., Yi Z., Hagiwara N., Monzen K., Komuro I., Brilliant M.H.
Nucleic Acids Res. 31:5941-5948(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DAZAP2.
[8]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U32614 mRNA. Translation: AAC52263.1.
D61689 mRNA. Translation: BAA09618.1.
AJ010605 mRNA. Translation: CAA09270.1.
X65659 mRNA. Translation: CAA46610.1.
PIRS22944.
S59121.
RefSeqNP_001264255.1. NM_001277326.1.
NP_001264257.1. NM_001277328.1.
NP_035575.1. NM_011445.4.
XP_006507569.1. XM_006507506.1.
XP_006507570.1. XM_006507507.1.
XP_006507573.1. XM_006507510.1.
XP_006507574.1. XM_006507511.1.
UniGeneMm.323365.
Mm.487065.

3D structure databases

ProteinModelPortalP40645.
SMRP40645. Positions 618-687.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203410. 5 interactions.
IntActP40645. 1 interaction.

PTM databases

PhosphoSiteP40645.

Proteomic databases

PRIDEP40645.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000072804; ENSMUSP00000072583; ENSMUSG00000051910. [P40645-1]
ENSMUST00000166207; ENSMUSP00000129027; ENSMUSG00000051910. [P40645-1]
GeneID20679.
KEGGmmu:20679.
UCSCuc009jin.1. mouse. [P40645-2]
uc009jip.1. mouse. [P40645-1]

Organism-specific databases

CTD55553.
MGIMGI:98368. Sox6.

Phylogenomic databases

eggNOGNOG253815.
HOGENOMHOG000056455.
HOVERGENHBG003915.
InParanoidP40645.
KOK09269.
OMAFENLGPQ.
OrthoDBEOG70087H.
PhylomeDBP40645.
TreeFamTF320471.

Gene expression databases

ArrayExpressP40645.
BgeeP40645.
CleanExMM_SOX6.
GenevestigatorP40645.

Family and domain databases

Gene3D1.10.30.10. 1 hit.
InterProIPR009071. HMG_box_dom.
[Graphical view]
PfamPF00505. HMG_box. 1 hit.
[Graphical view]
SMARTSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMSSF47095. SSF47095. 1 hit.
PROSITEPS50118. HMG_BOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSOX6. mouse.
NextBio299177.
PROP40645.
SOURCESearch...

Entry information

Entry nameSOX6_MOUSE
AccessionPrimary (citable) accession number: P40645
Secondary accession number(s): Q62250, Q9QWS5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot