P40645 (SOX6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor SOX-6

Alternative name(s):
SOX-LZ

Gene names

Name:	Sox6
Synonyms:	Sox-6

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	827 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation.
Subunit structure	Interacts with DAZAP2. May interact with CENPK. Ref.6 Ref.7
Subcellular location	Nucleus By similarity.
Tissue specificity	Highly expressed in testis.
Post-translational modification	Sumoylation inhibits the transcriptional activity By similarity.
Sequence similarities	Contains 1 HMG box DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	DNA-binding
Molecular function	Activator Developmental protein
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cardiocyte differentiation Inferred from direct assay Ref.7. Source: MGI cartilage development Inferred from genetic interaction PubMed 15634692. Source: MGI cell morphogenesis Inferred from mutant phenotype PubMed 16462943. Source: MGI cellular response to transforming growth factor beta stimulus Inferred from electronic annotation. Source: Compara erythrocyte development Inferred from mutant phenotype PubMed 16462943. Source: MGI gene silencing Inferred from mutant phenotype PubMed 16462943. Source: MGI in utero embryonic development Inferred from mutant phenotype PubMed 17084361. Source: MGI muscle cell differentiation Inferred from direct assay Ref.7. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 16462943. Source: MGI oligodendrocyte cell fate specification Inferred from mutant phenotype PubMed 17084361. Source: MGI positive regulation of cartilage development Inferred from electronic annotation. Source: Compara positive regulation of chondrocyte differentiation Inferred from direct assay PubMed 20940257. Source: UniProtKB positive regulation of mesenchymal stem cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from genetic interaction Ref.3. Source: MGI post-embryonic development Inferred from mutant phenotype PubMed 16462943. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	transcription factor complex Inferred by curator Ref.7. Source: MGI
Molecular_function	sequence-specific DNA binding Inferred from direct assay PubMed 16462943. Source: MGI sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 12446692PubMed 17084361. Source: MGI transcription regulatory region DNA binding Inferred from direct assay PubMed 17084361. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P40645-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P40645-2)

The sequence of this isoform differs from the canonical sequence as follows:
     327-367: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 827	827	Transcription factor SOX-6		PRO_0000048730
Regions
	DNA binding	620 – 688	69	HMG box
	Coiled coil	184 – 262	79	Potential
	Compositional bias	233 – 261	29	Gln-rich
	Compositional bias	240 – 243	4	Poly-Gln
	Compositional bias	280 – 285	6	Poly-Ala
	Compositional bias	313 – 317	5	Poly-Ala
	Compositional bias	514 – 517	4	Poly-Gln
Amino acid modifications
	Modified residue	126	1	Phosphoserine Ref.8
	Cross-link	404		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
	Cross-link	417		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Natural variations
	Alternative sequence	327 – 367	41	Missing in isoform 2.		VSP_002198
Experimental info
	Sequence conflict	312 – 313	2	MA → SS in BAA09618. Ref.2
	Sequence conflict	632	1	K → R in CAA46610. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: F777BAB7CFB1E93B	FASTA	827	91,803
	10 20 30 40 50 60 MSSKQATSPF ACTADGEEAM TQDLTSREKE EGSDQHPASH LPLHPIMHNK PHSEELPTLV 70 80 90 100 110 120 STIQQDADWD SVLSSQQRME SENNKLCSLY SFRNTSTSPH KPDEGSRERE IMNSVTFGTP 130 140 150 160 170 180 ERRKGSLADV VDTLKQKKLE EMTRTEQEDS SCMEKLLSKD WKEKMERLNT SELLGEIKGT 190 200 210 220 230 240 PESLAEKERQ LSTMITQLIS LREQLLAAHD EQKKLAASQI EKQRQQMDLA RQQQEQIARQ 250 260 270 280 290 300 QQQLLQQQHK INLLQQQIQV QGHMPPLMIP IFPHDQRTLA AAAAAQQGFL FPPGITYKPG 310 320 330 340 350 360 DNYPVQFIPS TMAAAAASGL SPLQLQKGHV SHPQINPRLK GISDRFGRNL DPSEHGGGHS 370 380 390 400 410 420 YNHRQIEQLY AAQLASMQVS PGAKMPSTPQ PPNSAGAVSP TGIKNEKRGT SPVTQVKDET 430 440 450 460 470 480 TAQPLNLSSR PKTAEPVKSP TSPTQNLFPA SKTSPVNLPN KSSIPSPIGG SLGRGSSLDI 490 500 510 520 530 540 LSSLNSPALF GDQDTVMKAI QEARKMREQI QREQQQQPHG VDGKLSSMNN MGLSNCRTEK 550 560 570 580 590 600 ERTRFENLGP QLTGKSSEDG KLGPGVIDLT RPEDAEGSKA MNGSAAKLQQ YYCWPTGGAT 610 620 630 640 650 660 VAEARVYRDA RGRASSEPHI KRPMNAFMVW AKDERRKILQ AFPDMHNSNI SKILGSRWKS 670 680 690 700 710 720 MSNQEKQPYY EEQARLSKIH LEKYPNYKYK PRPKRTCIVD GKKLRIGEYK QLMRSRRQEM 730 740 750 760 770 780 RQFFTVGQQP QMPITTGTGV VYPGAITMAT TTPSPQMTSD CSSTSASPEP SLPVIQSTYG 790 800 810 820 MKMDGASLAG NDMINGEDEM EAYDDYEDDP KSDYSSENEA PEPVSAN
	Isoform 2 [UniParc]. Checksum: EFC8E350AEC84653	FASTA	786	87,192
	10 20 30 40 50 60 MSSKQATSPF ACTADGEEAM TQDLTSREKE EGSDQHPASH LPLHPIMHNK PHSEELPTLV 70 80 90 100 110 120 STIQQDADWD SVLSSQQRME SENNKLCSLY SFRNTSTSPH KPDEGSRERE IMNSVTFGTP 130 140 150 160 170 180 ERRKGSLADV VDTLKQKKLE EMTRTEQEDS SCMEKLLSKD WKEKMERLNT SELLGEIKGT 190 200 210 220 230 240 PESLAEKERQ LSTMITQLIS LREQLLAAHD EQKKLAASQI EKQRQQMDLA RQQQEQIARQ 250 260 270 280 290 300 QQQLLQQQHK INLLQQQIQV QGHMPPLMIP IFPHDQRTLA AAAAAQQGFL FPPGITYKPG 310 320 330 340 350 360 DNYPVQFIPS TMAAAAASGL SPLQLQQLYA AQLASMQVSP GAKMPSTPQP PNSAGAVSPT 370 380 390 400 410 420 GIKNEKRGTS PVTQVKDETT AQPLNLSSRP KTAEPVKSPT SPTQNLFPAS KTSPVNLPNK 430 440 450 460 470 480 SSIPSPIGGS LGRGSSLDIL SSLNSPALFG DQDTVMKAIQ EARKMREQIQ REQQQQPHGV 490 500 510 520 530 540 DGKLSSMNNM GLSNCRTEKE RTRFENLGPQ LTGKSSEDGK LGPGVIDLTR PEDAEGSKAM 550 560 570 580 590 600 NGSAAKLQQY YCWPTGGATV AEARVYRDAR GRASSEPHIK RPMNAFMVWA KDERRKILQA 610 620 630 640 650 660 FPDMHNSNIS KILGSRWKSM SNQEKQPYYE EQARLSKIHL EKYPNYKYKP RPKRTCIVDG 670 680 690 700 710 720 KKLRIGEYKQ LMRSRRQEMR QFFTVGQQPQ MPITTGTGVV YPGAITMATT TPSPQMTSDC 730 740 750 760 770 780 SSTSASPEPS LPVIQSTYGM KMDGASLAGN DMINGEDEME AYDDYEDDPK SDYSSENEAP EPVSAN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Sry-related HMG box-containing gene Sox6 is expressed in the adult testis and developing nervous system of the mouse." Connor F., Wright E., Denny P., Koopman P., Ashworth A. Nucleic Acids Res. 23:3365-3372(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Testis.
[2]	"A gene that is related to SRY and is expressed in the testes encodes a leucine zipper-containing protein." Takamatsu N., Kanda H., Tsuchiya I., Yamada S., Ito M., Kabeno S., Shiba T., Yamashita S. Mol. Cell. Biol. 15:3759-3766(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Testis.
[3]	"A new long form of Sox5 (L-Sox5), Sox6 and Sox9 are coexpressed in chondrogenesis and cooperatively activate the type II collagen gene." Lefebvre V., Li P., de Crombrugghe B. EMBO J. 17:5718-5733(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 519-827.
[4]	"A conserved family of genes related to the testis determining gene, SRY." Denny P., Swift S., Brand N., Dabhade N., Barton P., Ashworth A. Nucleic Acids Res. 20:2887-2887(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 631-684. Strain: Parkes. Tissue: Brain and Testis.
[5]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 660-666, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
[6]	"Characterization of Solt, a novel SoxLZ/Sox6 binding protein expressed in adult mouse testis." Yamashita A., Ito M., Takamatsu N., Shiba T. FEBS Lett. 481:147-151(2000) [PubMed] [Europe PMC] [Abstract] Cited for: POSSIBLE INTERACTION WITH CENPK.
[7]	"Sox6 regulation of cardiac myocyte development." Cohen-Barak O., Yi Z., Hagiwara N., Monzen K., Komuro I., Brilliant M.H. Nucleic Acids Res. 31:5941-5948(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH DAZAP2.
[8]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-126, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U32614 mRNA. Translation: AAC52263.1. D61689 mRNA. Translation: BAA09618.1. AJ010605 mRNA. Translation: CAA09270.1. X65659 mRNA. Translation: CAA46610.1.
IPI	IPI00620986. IPI00623688.
PIR	S22944. S59121.
RefSeq	NP_001264255.1. NM_001277326.1. NP_001264257.1. NM_001277328.1. NP_035575.1. NM_011445.4.
UniGene	Mm.323365.
3D structure databases
ProteinModelPortal	P40645.
SMR	P40645. Positions 618-687.
ModBase	Search...
Protein-protein interaction databases
IntAct	P40645. 1 interaction.
PTM databases
PhosphoSite	P40645.
Proteomic databases
PRIDE	P40645.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000072804; ENSMUSP00000072583; ENSMUSG00000051910. ENSMUST00000166207; ENSMUSP00000129027; ENSMUSG00000051910.
GeneID	20679.
KEGG	mmu:20679.
UCSC	uc009jin.1. mouse. uc009jip.1. mouse.
Organism-specific databases
CTD	55553.
MGI	MGI:98368. Sox6.
Phylogenomic databases
eggNOG	NOG253815.
HOGENOM	HOG000056455.
HOVERGEN	HBG003915.
InParanoid	P40645.
KO	K09269.
OMA	FENLGPQ.
OrthoDB	EOG4N8R45.
Gene expression databases
ArrayExpress	P40645.
Bgee	P40645.
CleanEx	MM_SOX6.
Genevestigator	P40645.
GermOnline	ENSMUSG00000051910. Mus musculus.
Family and domain databases
Gene3D	1.10.30.10. 1 hit.
InterPro	IPR009071. HMG_box_dom. IPR027153. Sox-6. [Graphical view]
PANTHER	PTHR10270:SF89. PTHR10270:SF89. 1 hit.
Pfam	PF00505. HMG_box. 1 hit. [Graphical view]
SMART	SM00398. HMG. 1 hit. [Graphical view]
SUPFAM	SSF47095. HMG-box. 1 hit.
PROSITE	PS50118. HMG_BOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SOX6. mouse.
NextBio	299177.
SOURCE	Search...

Entry information

Entry name

SOX6_MOUSE

Accession

Primary (citable) accession number: P40645
Secondary accession number(s): Q62250, Q9QWS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1996
Last modified:	May 29, 2013
This is version 113 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program