Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P40630

- TFAM_MOUSE

UniProt

P40630 - TFAM_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Transcription factor A, mitochondrial

Gene

Tfam

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Isoform Mitochondrial binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. Isoform Nuclear may also function as a transcriptional activator or may have a structural role in the compaction of nuclear DNA during spermatogenesis.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei57 – 571Intercalates between bases and promotes DNA bendingBy similarity
Sitei181 – 1811Intercalates between bases and promotes DNA bendingBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi49 – 11769HMG box 1PROSITE-ProRule annotationAdd
BLAST
DNA bindingi154 – 21865HMG box 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: UniProtKB
  2. DNA binding, bending Source: UniProtKB
  3. mitochondrial light strand promoter sense binding Source: UniProtKB
  4. poly(A) RNA binding Source: Ensembl
  5. sequence-specific DNA binding transcription factor activity Source: UniProtKB

GO - Biological processi

  1. mitochondrial respiratory chain complex assembly Source: MGI
  2. positive regulation of transcription, DNA-templated Source: UniProtKB
  3. transcription from mitochondrial promoter Source: UniProtKB
  4. transcription initiation from mitochondrial promoter Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_205251. Transcriptional activation of mitochondrial biogenesis.
REACT_238550. Mitochondrial transcription initiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor A, mitochondrial
Short name:
mtTFA
Alternative name(s):
Testis-specific high mobility group protein
Short name:
TS-HMG
Gene namesi
Name:Tfam
Synonyms:Hmgts
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:107810. Tfam.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial nucleoid Source: UniProtKB
  2. mitochondrion Source: MGI
  3. nucleus Source: UniProtKB-KW
  4. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Mitochondrion nucleoid, Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethal, due to absence of mitochondrial DNA. Mutant embryos die before 10.5 dpc.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4242MitochondrionSequence AnalysisAdd
BLAST
Chaini43 – 243201Transcription factor A, mitochondrialPRO_0000013471Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei54 – 541Phosphoserine; by PKABy similarity
Modified residuei55 – 551Phosphoserine; by PKABy similarity
Modified residuei60 – 601Phosphoserine; by PKABy similarity
Modified residuei66 – 661N6-succinyllysine1 Publication
Modified residuei159 – 1591Phosphoserine; by PKABy similarity
Modified residuei192 – 1921PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation by PKA within the HMG box 1 impairs DNA binding and promotes degradation by the AAA+ Lon protease.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40630.
PaxDbiP40630.
PRIDEiP40630.

PTM databases

PhosphoSiteiP40630.

Expressioni

Tissue specificityi

The mitochondrial isoform is widely expressed while the nuclear isoform is testis-specific.

Gene expression databases

BgeeiP40630.
CleanExiMM_TFAM.
ExpressionAtlasiP40630. baseline and differential.
GenevestigatoriP40630.

Interactioni

Subunit structurei

Monomer; binds DNA as a monomer. Interacts with TFB1M and TFB2M. Interacts with CLPX; this enhances DNA-binding (By similarity).By similarity

Protein-protein interaction databases

BioGridi204137. 2 interactions.
IntActiP40630. 4 interactions.
MINTiMINT-1861500.

Structurei

3D structure databases

ProteinModelPortaliP40630.
SMRiP40630. Positions 43-232.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Binds DNA via its HMG boxes. When bound to the mitochondrial light strand promoter, bends DNA into a U-turn shape, each HMG box bending the DNA by 90 degrees (By similarity).By similarity

Sequence similaritiesi

Contains 2 HMG box DNA-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiNOG297801.
GeneTreeiENSGT00440000039001.
HOGENOMiHOG000139423.
HOVERGENiHBG106674.
InParanoidiP40630.
KOiK11830.
OMAiDDKIRYE.
OrthoDBiEOG7CG724.
PhylomeDBiP40630.
TreeFamiTF318343.

Family and domain databases

Gene3Di1.10.30.10. 2 hits.
InterProiIPR009071. HMG_box_dom.
[Graphical view]
PfamiPF00505. HMG_box. 1 hit.
PF09011. HMG_box_2. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 2 hits.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 2 hits.
PROSITEiPS50118. HMG_BOX_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Mitochondrial (identifier: P40630-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALFRGMWSV LKALGRTGVE MCAGCGGRIP SSISLVCIPK CFSSMGSYPK
60 70 80 90 100
KPMSSYLRFS TEQLPKFKAK HPDAKLSELV RKIAALWREL PEAEKKVYEA
110 120 130 140 150
DFKAEWKAYK EAVSKYKEQL TPSQLMGMEK EARQRRLKKK ALVKRRELIL
160 170 180 190 200
LGKPKRPRSA YNIYVSESFQ EAKDDSAQGK LKLVNEAWKN LSPEEKQAYI
210 220 230 240
QLAKDDRIRY DNEMKSWEEQ MAEVGRSDLI RRSVKRSGDI SEH
Length:243
Mass (Da):27,988
Last modified:August 30, 2002 - v2
Checksum:i3477F6EEAD9A3EBF
GO
Isoform Nuclear (identifier: P40630-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: Missing.
     29-34: IPSSIS → MAGAWG

Show »
Length:215
Mass (Da):25,036
Checksum:iB728ED1DF9053452
GO

Sequence cautioni

The sequence AAA02579.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AK004857 differs from that shown. Reason: Frameshift at position 195. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti34 – 341S → R in AAA02579. (PubMed:8321234)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2828Missing in isoform Nuclear. 1 PublicationVSP_002185Add
BLAST
Alternative sequencei29 – 346IPSSIS → MAGAWG in isoform Nuclear. 1 PublicationVSP_002186

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57939 mRNA. Translation: AAC52815.1.
U63858 mRNA. Translation: AAC52816.1.
BC001987 mRNA. Translation: AAH01987.1.
BC083084 mRNA. Translation: AAH83084.1.
AK004857 mRNA. No translation available.
AK050446 mRNA. Translation: BAC34258.1.
AK167348 mRNA. Translation: BAE39447.1.
AK167777 mRNA. Translation: BAE39809.1.
AK169808 mRNA. Translation: BAE41382.1.
U63712 mRNA. Translation: AAB06395.1.
L07107 mRNA. Translation: AAA02579.1. Different initiation.
CCDSiCCDS23916.1. [P40630-1]
PIRiI49745.
RefSeqiNP_033386.1. NM_009360.4. [P40630-1]
UniGeneiMm.229292.

Genome annotation databases

EnsembliENSMUST00000092430; ENSMUSP00000090086; ENSMUSG00000003923. [P40630-1]
ENSMUST00000105432; ENSMUSP00000101072; ENSMUSG00000003923. [P40630-2]
ENSMUST00000121685; ENSMUSP00000113581; ENSMUSG00000003923.
GeneIDi21780.
KEGGimmu:21780.
UCSCiuc007fol.1. mouse. [P40630-2]
uc007fom.1. mouse. [P40630-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57939 mRNA. Translation: AAC52815.1 .
U63858 mRNA. Translation: AAC52816.1 .
BC001987 mRNA. Translation: AAH01987.1 .
BC083084 mRNA. Translation: AAH83084.1 .
AK004857 mRNA. No translation available.
AK050446 mRNA. Translation: BAC34258.1 .
AK167348 mRNA. Translation: BAE39447.1 .
AK167777 mRNA. Translation: BAE39809.1 .
AK169808 mRNA. Translation: BAE41382.1 .
U63712 mRNA. Translation: AAB06395.1 .
L07107 mRNA. Translation: AAA02579.1 . Different initiation.
CCDSi CCDS23916.1. [P40630-1 ]
PIRi I49745.
RefSeqi NP_033386.1. NM_009360.4. [P40630-1 ]
UniGenei Mm.229292.

3D structure databases

ProteinModelPortali P40630.
SMRi P40630. Positions 43-232.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204137. 2 interactions.
IntActi P40630. 4 interactions.
MINTi MINT-1861500.

PTM databases

PhosphoSitei P40630.

Proteomic databases

MaxQBi P40630.
PaxDbi P40630.
PRIDEi P40630.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000092430 ; ENSMUSP00000090086 ; ENSMUSG00000003923 . [P40630-1 ]
ENSMUST00000105432 ; ENSMUSP00000101072 ; ENSMUSG00000003923 . [P40630-2 ]
ENSMUST00000121685 ; ENSMUSP00000113581 ; ENSMUSG00000003923 .
GeneIDi 21780.
KEGGi mmu:21780.
UCSCi uc007fol.1. mouse. [P40630-2 ]
uc007fom.1. mouse. [P40630-1 ]

Organism-specific databases

CTDi 7019.
MGIi MGI:107810. Tfam.

Phylogenomic databases

eggNOGi NOG297801.
GeneTreei ENSGT00440000039001.
HOGENOMi HOG000139423.
HOVERGENi HBG106674.
InParanoidi P40630.
KOi K11830.
OMAi DDKIRYE.
OrthoDBi EOG7CG724.
PhylomeDBi P40630.
TreeFami TF318343.

Enzyme and pathway databases

Reactomei REACT_205251. Transcriptional activation of mitochondrial biogenesis.
REACT_238550. Mitochondrial transcription initiation.

Miscellaneous databases

ChiTaRSi Tfam. mouse.
NextBioi 301118.
PROi P40630.
SOURCEi Search...

Gene expression databases

Bgeei P40630.
CleanExi MM_TFAM.
ExpressionAtlasi P40630. baseline and differential.
Genevestigatori P40630.

Family and domain databases

Gene3Di 1.10.30.10. 2 hits.
InterProi IPR009071. HMG_box_dom.
[Graphical view ]
Pfami PF00505. HMG_box. 1 hit.
PF09011. HMG_box_2. 1 hit.
[Graphical view ]
SMARTi SM00398. HMG. 2 hits.
[Graphical view ]
SUPFAMi SSF47095. SSF47095. 2 hits.
PROSITEi PS50118. HMG_BOX_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A single mouse gene encodes the mitochondrial transcription factor A and a testis-specific nuclear HMG-box protein."
    Larsson N.G., Garman J.D., Oldfors A., Barsh G.S., Clayton D.A.
    Nat. Genet. 13:296-302(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS MITOCHONDRIAL AND NUCLEAR), FUNCTION, ALTERNATIVE SPLICING.
    Tissue: Lymphocyte and Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM MITOCHONDRIAL).
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM MITOCHONDRIAL).
    Strain: C57BL/6J, DBA/2 and NOD.
    Tissue: Liver and Thymus.
  4. Cermakian N., Cedergren R.
    Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-48 (ISOFORM MITOCHONDRIAL).
    Strain: CD-1.
    Tissue: Testis.
  5. "A testis-specific gene encoding a nuclear high-mobility-group box protein located in elongating spermatids."
    Boissonneault G., Lau Y.-F.C.
    Mol. Cell. Biol. 13:4323-4330(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 34-243 (ISOFORM MITOCHONDRIAL).
    Strain: BALB/c.
    Tissue: Testis.
  6. "Mitochondrial transcription factor A is necessary for mtDNA maintenance and embryogenesis in mice."
    Larsson N.G., Wang J., Wilhelmsson H., Oldfors A., Rustin P., Lewandoski M., Barsh G.S., Clayton D.A.
    Nat. Genet. 18:231-236(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. "The mitochondrial transcription factor TFAM coordinates the assembly of multiple DNA molecules into nucleoid-like structures."
    Kaufman B.A., Durisic N., Mativetsky J.M., Costantino S., Hancock M.A., Grutter P., Shoubridge E.A.
    Mol. Biol. Cell 18:3225-3236(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  8. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-66, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiTFAM_MOUSE
AccessioniPrimary (citable) accession number: P40630
Secondary accession number(s): P97894
, P97906, Q543I8, Q9DBM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: August 30, 2002
Last modified: November 26, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3