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Protein

High mobility group protein B3

Gene

HMGB3

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (By similarity). Binds to the delta-1 crystallin/ASL1 enhancer (PubMed:7904558). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi9 – 79HMG box 1PROSITE-ProRule annotationAdd BLAST71
DNA bindingi93 – 161HMG box 2PROSITE-ProRule annotationAdd BLAST69

GO - Molecular functioni

  • DNA binding, bending Source: AgBase
  • four-way junction DNA binding Source: AgBase
  • RNA binding Source: Ensembl

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processImmunity, Innate immunity, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
High mobility group protein B3
Alternative name(s):
High mobility group protein 2a
Short name:
HMG-2a
High mobility group protein 4
Short name:
HMG-4
Gene namesi
Name:HMGB3
Synonyms:HMG2A, HMG4
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000485412 – 202High mobility group protein B3Add BLAST201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi23 ↔ 45In disulfide HMGB3By similarity
Modified residuei23Cysteine sulfonic acid (-SO3H); alternateBy similarity1
Modified residuei45Cysteine sulfonic acid (-SO3H); alternateBy similarity1
Modified residuei104Cysteine sulfonic acid (-SO3H)By similarity1

Post-translational modificationi

Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-104 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB3 (HMGB3C23hC45hC104h), 2- disulfide HMGB3 (HMGB3C23-C45C104h) and 3- sulfonyl HMGB3 (HMGB3C23soC45soC104so).By similarity

Keywords - PTMi

Disulfide bond, Oxidation

Proteomic databases

PaxDbiP40618

Expressioni

Developmental stagei

Found in newly hatched chick liver and decreases during postnatal development.

Gene expression databases

BgeeiENSGALG00000009071
ExpressionAtlasiP40618 baseline and differential

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000014747

Structurei

3D structure databases

SMRiP40618
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi182 – 202Asp/Glu-rich (acidic)Add BLAST21

Sequence similaritiesi

Belongs to the HMGB family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00760000119164
HOVERGENiHBG009000
InParanoidiP40618
KOiK11296
OrthoDBiEOG091G0P81
TreeFamiTF105371

Family and domain databases

Gene3Di1.10.30.10, 2 hits
InterProiView protein in InterPro
IPR009071 HMG_box_dom
IPR036910 HMG_box_dom_sf
IPR017967 HMG_boxA_CS
IPR031077 HMGB3
PANTHERiPTHR13711:SF144 PTHR13711:SF144, 1 hit
PfamiView protein in Pfam
PF00505 HMG_box, 1 hit
PF09011 HMG_box_2, 1 hit
SMARTiView protein in SMART
SM00398 HMG, 2 hits
SUPFAMiSSF47095 SSF47095, 2 hits
PROSITEiView protein in PROSITE
PS00353 HMG_BOX_1, 1 hit
PS50118 HMG_BOX_2, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40618-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKGDPKKPK GKMSAYAFFV QTCREEHKKK NPEVPVNFAE FSKKCSERWK
60 70 80 90 100
TMSSKEKAKF DEMAKADKVR YDREMKDYGP AKGGKKKKDP NAPKRPPSGF
110 120 130 140 150
FLFCSEFRPK IKSTNPGISI GDVAKKLGEM WNNLSDGEKQ PYNNKAAKLK
160 170 180 190 200
EKYEKDVADY KSKGKFDGAK GAATKAARKK VEEEDEEEEE DEEEEDEDDD

DE
Length:202
Mass (Da):23,057
Last modified:September 18, 2013 - v3
Checksum:i24222C692BC5C903
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30Missing in BAA03260 (PubMed:7904558).Curated1
Sequence conflicti99G → A in CAA45065 (PubMed:1562600).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63463 mRNA Translation: CAA45065.1
AADN03004033 Genomic DNA No translation available.
AADN03004127 Genomic DNA No translation available.
D14314 mRNA Translation: BAA03260.1
PIRiS22359
RefSeqiNP_990626.2, NM_205295.1
UniGeneiGga.4513

Genome annotation databases

EnsembliENSGALT00000014763; ENSGALP00000014747; ENSGALG00000009071
GeneIDi396232
KEGGigga:396232

Similar proteinsi

Entry informationi

Entry nameiHMGB3_CHICK
AccessioniPrimary (citable) accession number: P40618
Secondary accession number(s): F1NGP8, P36194
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: September 18, 2013
Last modified: May 23, 2018
This is version 112 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health