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Protein

ADP-ribosylation factor-like protein 4A

Gene

ARL4A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in GDP-bound form.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi27 – 348GTPBy similarity
Nucleotide bindingi75 – 795GTPBy similarity
Nucleotide bindingi134 – 1374GTPBy similarity

GO - Molecular functioni

  • GTP binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-like protein 4A
Gene namesi
Name:ARL4A
Synonyms:ARL4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:695. ARL4A.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • nucleolus Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi34 – 341T → N: Inhibits relocalization of CYTH2 to the plasma membrane. Strongly localized in the nucleolus. 2 Publications
Mutagenesisi79 – 791Q → L: Localized in the nucleus and nucleolus. 2 Publications

Organism-specific databases

PharmGKBiPA24988.

Polymorphism and mutation databases

BioMutaiARL4A.
DMDMi1168495.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedSequence analysis
Chaini2 – 200199ADP-ribosylation factor-like protein 4APRO_0000207459Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence analysis

Post-translational modificationi

Myristoylated.1 Publication

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP40617.
PRIDEiP40617.

PTM databases

iPTMnetiP40617.
PhosphoSiteiP40617.

Expressioni

Gene expression databases

BgeeiP40617.
CleanExiHS_ARL4A.
ExpressionAtlasiP40617. baseline and differential.
GenevisibleiP40617. HS.

Interactioni

Subunit structurei

Interacts with CYTH2. Interacts with KPNA2; the interaction is direct. Does not interacts with ARL4A.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CCDC102BA1A4H13EBI-2875746,EBI-10171570
CCDC57Q2TAC23EBI-2875746,EBI-2808286
GOLGA2Q083793EBI-2875746,EBI-618309
SPATC1LQ9H0A93EBI-2875746,EBI-372911

Protein-protein interaction databases

BioGridi115428. 6 interactions.
IntActiP40617. 5 interactions.
STRINGi9606.ENSP00000349250.

Structurei

3D structure databases

ProteinModelPortaliP40617.
SMRiP40617. Positions 23-182.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00840000129731.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP40617.
KOiK07945.
OMAiCFHIVIL.
OrthoDBiEOG7H793N.
PhylomeDBiP40617.
TreeFamiTF105464.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40617-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNGLSDQTS ILSNLPSFQS FHIVILGLDC AGKTTVLYRL QFNEFVNTVP
60 70 80 90 100
TKGFNTEKIK VTLGNSKTVT FHFWDVGGQE KLRPLWKSYT RCTDGIVFVV
110 120 130 140 150
DSVDVERMEE AKTELHKITR ISENQGVPVL IVANKQDLRN SLSLSEIEKL
160 170 180 190 200
LAMGELSSST PWHLQPTCAI IGDGLKEGLE KLHDMIIKRR KMLRQQKKKR
Length:200
Mass (Da):22,615
Last modified:November 1, 1995 - v2
Checksum:i8D93A8C003BD16CF
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti139 – 1391R → K.
Corresponds to variant rs2953325 [ dbSNP | Ensembl ].
VAR_024367

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73960 mRNA. Translation: AAB39713.1.
AK313233 mRNA. Translation: BAG36044.1.
AF493890 mRNA. Translation: AAM12604.1.
CH236948 Genomic DNA. Translation: EAL24295.1.
CH471073 Genomic DNA. Translation: EAW93650.1.
BC001111 mRNA. Translation: AAH01111.1.
BC003027 mRNA. Translation: AAH03027.1.
CCDSiCCDS5359.1.
RefSeqiNP_001032241.1. NM_001037164.2.
NP_001182325.1. NM_001195396.1.
NP_005729.1. NM_005738.4.
NP_997625.1. NM_212460.3.
UniGeneiHs.245540.

Genome annotation databases

EnsembliENST00000356797; ENSP00000349250; ENSG00000122644.
ENST00000396662; ENSP00000379897; ENSG00000122644.
ENST00000396663; ENSP00000379898; ENSG00000122644.
ENST00000396664; ENSP00000379899; ENSG00000122644.
ENST00000404894; ENSP00000385236; ENSG00000122644.
GeneIDi10124.
KEGGihsa:10124.
UCSCiuc003ssp.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73960 mRNA. Translation: AAB39713.1.
AK313233 mRNA. Translation: BAG36044.1.
AF493890 mRNA. Translation: AAM12604.1.
CH236948 Genomic DNA. Translation: EAL24295.1.
CH471073 Genomic DNA. Translation: EAW93650.1.
BC001111 mRNA. Translation: AAH01111.1.
BC003027 mRNA. Translation: AAH03027.1.
CCDSiCCDS5359.1.
RefSeqiNP_001032241.1. NM_001037164.2.
NP_001182325.1. NM_001195396.1.
NP_005729.1. NM_005738.4.
NP_997625.1. NM_212460.3.
UniGeneiHs.245540.

3D structure databases

ProteinModelPortaliP40617.
SMRiP40617. Positions 23-182.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115428. 6 interactions.
IntActiP40617. 5 interactions.
STRINGi9606.ENSP00000349250.

PTM databases

iPTMnetiP40617.
PhosphoSiteiP40617.

Polymorphism and mutation databases

BioMutaiARL4A.
DMDMi1168495.

Proteomic databases

PaxDbiP40617.
PRIDEiP40617.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000356797; ENSP00000349250; ENSG00000122644.
ENST00000396662; ENSP00000379897; ENSG00000122644.
ENST00000396663; ENSP00000379898; ENSG00000122644.
ENST00000396664; ENSP00000379899; ENSG00000122644.
ENST00000404894; ENSP00000385236; ENSG00000122644.
GeneIDi10124.
KEGGihsa:10124.
UCSCiuc003ssp.4. human.

Organism-specific databases

CTDi10124.
GeneCardsiARL4A.
HGNCiHGNC:695. ARL4A.
MIMi604786. gene.
neXtProtiNX_P40617.
PharmGKBiPA24988.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00840000129731.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP40617.
KOiK07945.
OMAiCFHIVIL.
OrthoDBiEOG7H793N.
PhylomeDBiP40617.
TreeFamiTF105464.

Miscellaneous databases

GeneWikiiARL4A.
GenomeRNAii10124.
NextBioi38291.
PROiP40617.
SOURCEiSearch...

Gene expression databases

BgeeiP40617.
CleanExiHS_ARL4A.
ExpressionAtlasiP40617. baseline and differential.
GenevisibleiP40617. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Rosenwald A.G., Kahn R.A.
    Submitted (JAN-1995) to UniProtKB
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Umbilical vein endothelial cell.
  2. Choe I.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Puhl H.L. III, Ikeda S.R., Aronstam R.S.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. "Human chromosome 7: DNA sequence and biology."
    Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
    , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
    Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Melanoma.
  8. "ADP-ribosylation factor (ARF)-like 4, 6, and 7 represent a subgroup of the ARF family characterization by rapid nucleotide exchange and a nuclear localization signal."
    Jacobs S., Schilf C., Fliegert F., Koling S., Weber Y., Schurmann A., Joost H.-G.
    FEBS Lett. 456:384-388(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  9. "ARL4, an ARF-like protein that is developmentally regulated and localized to nuclei and nucleoli."
    Lin C.Y., Huang P.H., Liao W.L., Cheng H.J., Huang C.F., Kuo J.C., Patton W.A., Massenburg D., Moss J., Lee F.J.
    J. Biol. Chem. 275:37815-37823(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MYRISTOYLATION, INTERACTION WITH KPNA2, MUTAGENESIS OF THR-34 AND GLN-79, SUBCELLULAR LOCATION.
  10. "ADP ribosylation factor-like protein 2 (Arl2) regulates the interaction of tubulin-folding cofactor D with native tubulin."
    Bhamidipati A., Lewis S.A., Cowan N.J.
    J. Cell Biol. 149:1087-1096(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: ABSENCE OF INTERACTION WITH ARL4A.
  11. "The Arl4 family of small G proteins can recruit the cytohesin Arf6 exchange factors to the plasma membrane."
    Hofmann I., Thompson A., Sanderson C.M., Munro S.
    Curr. Biol. 17:711-716(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CYTH2, SUBCELLULAR LOCATION, MUTAGENESIS OF THR-34 AND GLN-79.

Entry informationi

Entry nameiARL4A_HUMAN
AccessioniPrimary (citable) accession number: P40617
Secondary accession number(s): A4D119, P80418, Q49AF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1995
Last modified: May 11, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.