P40617 (ARL4A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADP-ribosylation factor-like protein 4A

Gene names

Name:	ARL4A
Synonyms:	ARL4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in GDP-bound form. Ref.9 Ref.11
Subunit structure	Interacts with CYTH2. Interacts with KPNA2; the interaction is direct. Does not interacts with ARL4A. Ref.9 Ref.10 Ref.11
Subcellular location	Cell membrane. Cytoplasm. Nucleus › nucleolus. Note: Localization in the nucleolus is dependent by nucleotide binding. Ref.8 Ref.9 Ref.11
Post-translational modification	Myristoylated.
Sequence similarities	Belongs to the small GTPase superfamily. Arf family.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane Nucleus
Coding sequence diversity	Polymorphism
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Myristate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from direct assay Ref.9. Source: UniProtKB plasma membrane Inferred from direct assay Ref.11. Source: UniProtKB
Molecular function	GTP binding Inferred from direct assay Ref.9. Source: UniProtKB protein binding Inferred from physical interaction Ref.9Ref.11. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Potential
	Chain	2 – 200	199	ADP-ribosylation factor-like protein 4A		PRO_0000207459
Regions
	Nucleotide binding	27 – 34	8	GTP By similarity
	Nucleotide binding	75 – 79	5	GTP By similarity
	Nucleotide binding	134 – 137	4	GTP By similarity
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine Potential
Natural variations
	Natural variant	139	1	R → K. Corresponds to variant rs2953325 [ dbSNP | Ensembl ].		VAR_024367
Experimental info
	Mutagenesis	34	1	T → N: Inhibits relocalization of CYTH2 to the plasma membrane. Strongly localized in the nucleolus. Ref.9 Ref.11
	Mutagenesis	79	1	Q → L: Localized in the nucleus and nucleolus. Ref.9 Ref.11

Sequences

	Sequence		Length	Mass (Da)
	P40617 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 8D93A8C003BD16CF	FASTA	200	22,615
	10 20 30 40 50 60 MGNGLSDQTS ILSNLPSFQS FHIVILGLDC AGKTTVLYRL QFNEFVNTVP TKGFNTEKIK 70 80 90 100 110 120 VTLGNSKTVT FHFWDVGGQE KLRPLWKSYT RCTDGIVFVV DSVDVERMEE AKTELHKITR 130 140 150 160 170 180 ISENQGVPVL IVANKQDLRN SLSLSEIEKL LAMGELSSST PWHLQPTCAI IGDGLKEGLE 190 200 KLHDMIIKRR KMLRQQKKKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Rosenwald A.G., Kahn R.A. Submitted (JAN-1995) to UniProtKB Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Umbilical vein endothelial cell.
[2]	Choe I. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Human chromosome 7: DNA sequence and biology." Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C. Science 300:767-772(2003) [PubMed: 12690205] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Melanoma.
[8]	"ADP-ribosylation factor (ARF)-like 4, 6, and 7 represent a subgroup of the ARF family characterization by rapid nucleotide exchange and a nuclear localization signal." Jacobs S., Schilf C., Fliegert F., Koling S., Weber Y., Schurmann A., Joost H.-G. FEBS Lett. 456:384-388(1999) [PubMed: 10462049] [Abstract] Cited for: SUBCELLULAR LOCATION.
[9]	"ARL4, an ARF-like protein that is developmentally regulated and localized to nuclei and nucleoli." Lin C.Y., Huang P.H., Liao W.L., Cheng H.J., Huang C.F., Kuo J.C., Patton W.A., Massenburg D., Moss J., Lee F.J. J. Biol. Chem. 275:37815-37823(2000) [PubMed: 10980193] [Abstract] Cited for: FUNCTION, MYRISTOYLATION, INTERACTION WITH KPNA2, MUTAGENESIS OF THR-34 AND GLN-79, SUBCELLULAR LOCATION.
[10]	"ADP ribosylation factor-like protein 2 (Arl2) regulates the interaction of tubulin-folding cofactor D with native tubulin." Bhamidipati A., Lewis S.A., Cowan N.J. J. Cell Biol. 149:1087-1096(2000) [PubMed: 10831612] [Abstract] Cited for: ABSENCE OF INTERACTION WITH ARL4A.
[11]	"The Arl4 family of small G proteins can recruit the cytohesin Arf6 exchange factors to the plasma membrane." Hofmann I., Thompson A., Sanderson C.M., Munro S. Curr. Biol. 17:711-716(2007) [PubMed: 17398095] [Abstract] Cited for: FUNCTION, INTERACTION WITH CYTH2, SUBCELLULAR LOCATION, MUTAGENESIS OF THR-34 AND GLN-79.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U73960 mRNA. Translation: AAB39713.1. AK313233 mRNA. Translation: BAG36044.1. AF493890 mRNA. Translation: AAM12604.1. CH236948 Genomic DNA. Translation: EAL24295.1. CH471073 Genomic DNA. Translation: EAW93650.1. BC001111 mRNA. Translation: AAH01111.1. BC003027 mRNA. Translation: AAH03027.1.
IPI	IPI00029596.
RefSeq	NP_001032241.1. NM_001037164.2. NP_001182325.1. NM_001195396.1. NP_005729.1. NM_005738.4. NP_997625.1. NM_212460.3.
UniGene	Hs.245540. Hs.729252.
3D structure databases
ProteinModelPortal	P40617.
SMR	P40617. Positions 15-187.
ModBase	Search...
Protein-protein interaction databases
IntAct	P40617. 1 interaction.
STRING	P40617.
Polymorphism databases
DMDM	1168495.
Proteomic databases
PRIDE	P40617.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000356797; ENSP00000349250; ENSG00000122644. ENST00000396662; ENSP00000379897; ENSG00000122644. ENST00000396663; ENSP00000379898; ENSG00000122644. ENST00000396664; ENSP00000379899; ENSG00000122644. ENST00000404894; ENSP00000385236; ENSG00000122644.
GeneID	10124.
KEGG	hsa:10124.
UCSC	uc003ssp.1. human.
Organism-specific databases
CTD	10124.
GeneCards	GC07P012726.
H-InvDB	HIX0006491.
HGNC	HGNC:695. ARL4A.
MIM	604786. gene.
neXtProt	NX_P40617.
PharmGKB	PA24988.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00600000084228.
HOGENOM	HBG745225.
HOVERGEN	HBG002073.
InParanoid	P40617.
OMA	CFHIVIL.
OrthoDB	EOG483D5M.
PhylomeDB	P40617.
Gene expression databases
ArrayExpress	P40617.
Bgee	P40617.
CleanEx	HS_ARL4A.
Genevestigator	P40617.
GermOnline	ENSG00000122644. Homo sapiens.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR024156. Small_GTPase_ARF. IPR006689. Small_GTPase_ARF/SAR. [Graphical view]
KO	K07945.
PANTHER	PTHR11711. ARF/SAR. 1 hit.
Pfam	PF00025. Arf. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00177. ARF. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. Small_GTP. 1 hit.
PROSITE	PS51417. ARF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	38291.
SOURCE	Search...

Entry information

Entry name

ARL4A_HUMAN

Accession

Primary (citable) accession number: P40617
Secondary accession number(s): A4D119, P80418, Q49AF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.