Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P40615

- DKC1_RAT

UniProt

P40615 - DKC1_RAT

Protein

H/ACA ribonucleoprotein complex subunit 4

Gene

Dkc1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Required for telomere maintenance; necessary for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme By similarity. Also required for ribosome biogenesis. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs.By similarity1 Publication

    Catalytic activityi

    RNA uridine = RNA pseudouridine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei126 – 1261NucleophileBy similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. pseudouridine synthase activity Source: InterPro
    3. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. cell proliferation Source: RGD
    2. pseudouridine synthesis Source: InterPro
    3. rRNA processing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Isomerase, Ribonucleoprotein

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    H/ACA ribonucleoprotein complex subunit 4 (EC:5.4.99.-)
    Alternative name(s):
    Dyskerin
    Nopp140-associated protein of 57 kDa
    Nucleolar protein NAP57
    Nucleolar protein family A member 4
    snoRNP protein DKC1
    Gene namesi
    Name:Dkc1
    Synonyms:Nap57
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621780. Dkc1.

    Subcellular locationi

    Nucleusnucleolus. NucleusCajal body
    Note: Also localized to Cajal bodies (coiled bodies).

    GO - Cellular componenti

    1. Cajal body Source: RGD
    2. nucleolus Source: RGD
    3. nucleoplasm Source: RGD
    4. small nucleolar ribonucleoprotein complex Source: RGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 509508H/ACA ribonucleoprotein complex subunit 4PRO_0000121985Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei452 – 4521PhosphoserineBy similarity
    Modified residuei454 – 4541PhosphoserineBy similarity
    Modified residuei508 – 5081PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiP40615.

    PTM databases

    PhosphoSiteiP40615.

    Expressioni

    Gene expression databases

    GenevestigatoriP40615.

    Interactioni

    Subunit structurei

    May interact with TERC, which contains a 3'-terminal domain related to the box H/ACA snoRNAs. May interact with the SMN complex, consisting of SMN1 or SMN2, GEMIN2/SIP1, DDX20/GEMIN3, and GEMIN4. The SMN complex may be required for correct assembly of the H/ACA snoRNP complex. Component of the telomerase holoenzyme complex at least composed of TERT, DKC1, WRAP53/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and a telomerase RNA template component (TERC) By similarity. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which contains NHP2/NOLA2, GAR1/NOLA1, NOP10/NOLA3, and DKC1/NOLA4, which is presumed to be the catalytic subunit. The complex contains a stable core formed by binding of one or two NOP10-DKC1 heterodimers to NHP2; GAR1 subsequently binds to this core via DKC1. The complex binds a box H/ACA small nucleolar RNA (snoRNA), which may target the specific site of modification within the RNA substrate. During assembly, the complex contains NAF1 instead of GAR1/NOLA1. Specific interactions with snoRNAs or TERC are mediated by GAR1 and NHP2. Associates with NOLC1/NOPP140. Interacts with SHQ1; this interaction may lead to the stabilization of DKC1, from the time of its synthesis until its association with NOP10, NHP2, and NAF1 at the nascent H/ACA RNA By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LOC100360065D3ZMP64EBI-5746997,EBI-8792072
    NAF1Q96HR83EBI-5746997,EBI-2515597From a different organism.

    Protein-protein interaction databases

    IntActiP40615. 5 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP40615.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini297 – 37276PUAPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 2221Nucleolar localizationBy similarityAdd
    BLAST
    Regioni447 – 50963Nuclear and nucleolar localizationBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi12 – 187Poly-Lys

    Sequence similaritiesi

    Belongs to the pseudouridine synthase TruB family.Curated
    Contains 1 PUA domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG081442.
    KOiK11131.
    PhylomeDBiP40615.

    Family and domain databases

    Gene3Di2.30.130.10. 1 hit.
    InterProiIPR012960. Dyskerin-like.
    IPR002501. PsdUridine_synth.
    IPR020103. PsdUridine_synth_cat_dom.
    IPR002478. PUA.
    IPR015947. PUA-like_domain.
    IPR004802. tRNA_PsdUridine_synth_B_fam.
    IPR004521. Uncharacterised_CHP00451.
    [Graphical view]
    PANTHERiPTHR23127. PTHR23127. 1 hit.
    PfamiPF08068. DKCLD. 1 hit.
    PF01472. PUA. 1 hit.
    PF01509. TruB_N. 1 hit.
    [Graphical view]
    SMARTiSM00359. PUA. 1 hit.
    [Graphical view]
    SUPFAMiSSF55120. SSF55120. 1 hit.
    SSF88697. SSF88697. 1 hit.
    TIGRFAMsiTIGR00425. CBF5. 1 hit.
    TIGR00451. unchar_dom_2. 1 hit.
    PROSITEiPS50890. PUA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P40615-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADAEAAMTF PKKHKKKKER KPLPEADVAE IQHAEDFLIK PESKAAQLDT    50
    SQWPLLLKNF DRLNVRTTHY TPIPCGSNPL KREIGEYVRT GFINLDKPSN 100
    PSSHEVVAWI RRILRVEKTG HSGTLDPKVT GCLIVCIERA TRLVKSQQSA 150
    GKEYVGVVRL HNAIEGTAQL SRALETLTGA LFQRPPLIAA VKRQLRVRTI 200
    YESRVVEYDP ERRLGVFWVS CEAGTYIRTL CVHLGLLLGV GGQMQELRRV 250
    RSGVVGERDH MVTMHDVLDA QYLYDHHRDE SYLRRVVFPL EKLLTSHKRL 300
    VMKDSAVNAI CYGAKIMLPG LLRYEDGIEV NQEVVVITTK GEAVCVAIAL 350
    MTTAVISTCD HGVVAKIKRV IMERDTYPRK WGLGPKASQK KQLIKQGLLD 400
    KHGRPTDGTP ASWTRDYVDY SDSSKKATAA EATPGPGVTA DAASIVKRKR 450
    DSDSDADEAT PTTTPRVKKE KKKKKEKADG GEEAAEDGDG DATRKKKKKK 500
    ARAAEELSG 509
    Length:509
    Mass (Da):56,615
    Last modified:January 23, 2007 - v4
    Checksum:i038862EBBF2A1E2F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti459 – 50850ATPTT…AEELS → RRPLPRP AA sequence (PubMed:7798307)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z34922 mRNA. Translation: CAA84402.1.
    BC099832 mRNA. Translation: AAH99832.1.
    PIRiA55163.
    RefSeqiNP_596910.1. NM_133419.1.
    UniGeneiRn.4223.

    Genome annotation databases

    GeneIDi170944.
    KEGGirno:170944.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z34922 mRNA. Translation: CAA84402.1 .
    BC099832 mRNA. Translation: AAH99832.1 .
    PIRi A55163.
    RefSeqi NP_596910.1. NM_133419.1.
    UniGenei Rn.4223.

    3D structure databases

    ProteinModelPortali P40615.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P40615. 5 interactions.

    PTM databases

    PhosphoSitei P40615.

    Proteomic databases

    PRIDEi P40615.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 170944.
    KEGGi rno:170944.

    Organism-specific databases

    CTDi 1736.
    RGDi 621780. Dkc1.

    Phylogenomic databases

    HOVERGENi HBG081442.
    KOi K11131.
    PhylomeDBi P40615.

    Miscellaneous databases

    NextBioi 621465.
    PROi P40615.

    Gene expression databases

    Genevestigatori P40615.

    Family and domain databases

    Gene3Di 2.30.130.10. 1 hit.
    InterProi IPR012960. Dyskerin-like.
    IPR002501. PsdUridine_synth.
    IPR020103. PsdUridine_synth_cat_dom.
    IPR002478. PUA.
    IPR015947. PUA-like_domain.
    IPR004802. tRNA_PsdUridine_synth_B_fam.
    IPR004521. Uncharacterised_CHP00451.
    [Graphical view ]
    PANTHERi PTHR23127. PTHR23127. 1 hit.
    Pfami PF08068. DKCLD. 1 hit.
    PF01472. PUA. 1 hit.
    PF01509. TruB_N. 1 hit.
    [Graphical view ]
    SMARTi SM00359. PUA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55120. SSF55120. 1 hit.
    SSF88697. SSF88697. 1 hit.
    TIGRFAMsi TIGR00425. CBF5. 1 hit.
    TIGR00451. unchar_dom_2. 1 hit.
    PROSITEi PS50890. PUA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "NAP57, a mammalian nucleolar protein with a putative homolog in yeast and bacteria."
      Meier U., Blobel G.
      J. Cell Biol. 127:1505-1514(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 21-43; 83-96 AND 98-119, SUBCELLULAR LOCATION, INTERACTION WITH NOLC1.
      Tissue: Liver.
    2. Meier U.
      Submitted (DEC-1997) to UniProtKB
      Cited for: SEQUENCE REVISION TO C-TERMINUS.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.
    4. "Immunopurified small nucleolar ribonucleoprotein particles pseudouridylate rRNA independently of their association with phosphorylated Nopp140."
      Wang C., Query C.C., Meier U.T.
      Mol. Cell. Biol. 22:8457-8466(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH GAR1; NHP2; NOP10 AND NOLC1, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiDKC1_RAT
    AccessioniPrimary (citable) accession number: P40615
    Secondary accession number(s): Q499M9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 108 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3