Reviewed, UniProtKB/Swiss-Prot P40601 (LIP1_PHOLU)

Names and origin

Protein names

Recommended name:
    Lipase 1
    EC=3.1.1.3
Alternative name(s):
    Triacylglycerol lipase

Gene names

Name:

lip-1

Organism

Photorhabdus luminescens (Xenorhabdus luminescens)

Taxonomic identifier

29488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	645 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Secreted.
Sequence similarities	Belongs to the 'GDSL' lipolytic enzyme family. Contains 1 autotransporter (TC 1.B.12) domain. [View classification]

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell outer membrane Inferred from electronic annotation. Source: InterPro
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Ref.1
	Chain	25 – 645	621	Lipase 1		PRO_0000017843
Regions
	Domain	383 – 645	263	Autotransporter
Sites
	Active site	34	1	By similarity
	Active site	330	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	P40601-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 1271327B7C56932F	FASTA	645	70,717
	10 20 30 40 50 60 MKRSFIFAPG MLALSISAIS NAHAYNNLYV FGDSLSDGGN NGRYTVDGIN GTESKLYNDF 70 80 90 100 110 120 IAQQLGIELV NSKKGGTNYA AGGATAVADL NNKHNTQDQV MGYLASHSNR ADHNGMYVHW 130 140 150 160 170 180 IGGNDVDAAL RNPADAQKII TESAMAASSQ VHALLNAGAG LVIVPTVPDV GMTPKIMEFV 190 200 210 220 230 240 LSKGGATSKD LAKIHAVVNG YPTIDKDTRL QVIHGVFKQI GSDVSGGDAK KAEETTKQLI 250 260 270 280 290 300 DGYNELSSNA SKLVDNYNQL EDMALSQENG NIVRVDVNAL LHEVIANPLR YGFLNTIGYA 310 320 330 340 350 360 CAQGVNAGSC RSKDTGFDAS KPFLFADDFH PTPEAHHIVS QYTVSVLNAP YRVMLLTNAN 370 380 390 400 410 420 NVPVKGALAS LDGRLQQLRN VDNEQGKLGV FGGYSGNHSH TLTLGSDYQI MDNILLGGMI 430 440 450 460 470 480 SRYQDNSSPA DNFHYDGRGY VFTAYGLWRY YDKGWISGDL HYLDMKYEDI TRGIVLNDWL 490 500 510 520 530 540 RKENASTSGH QWGGRITAGW DIPLTSAVTT SPIIQYAWDK SYVKGYRESG NNSTAMHFGE 550 560 570 580 590 600 QRYDSQVGTL GWRLDTNFGY FNPYAEVRFN HQFGDKRYQI RSAINSTQTS FVSESQKQDT 610 620 630 640 HWREYTIGMN AVITKDWGAF ASISRNDGDV QNHTYSFSLG VNASF

References

[1]

"Phase variation in Xenorhabdus luminescens: cloning and sequencing of the lipase gene and analysis of its expression in primary and secondary phases of the bacterium." Wang H., Dowds B.C.A. J. Bacteriol. 175:1665-1673(1993) [PubMed: 8449874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 25-41. Strain: K122.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X66379 Genomic DNA. Translation: CAA47020.1.
PIR	A47081.
3D structure databases
ModBase	Search...
Genome annotation databases
UCSC	C05B10.1. c. elegans.
Enzyme and pathway databases
BRENDA	3.1.1.3. 257143.
Family and domain databases
InterPro	IPR005546. Auto_transptbeta. IPR017186. Lipase_autotranspt_EstA. IPR001087. Lipase_GDSL. IPR008265. Lipase_GDSL_AS. IPR006315. OM_autotransptr_brl. [Graphical view]
Pfam	PF03797. Autotransporter. 1 hit. PF00657. Lipase_GDSL. 1 hit. [Graphical view]
PIRSF	PIRSF037375. Autotrns_EstA. 1 hit.
TIGRFAMs	TIGR01414. autotrans_barl. 1 hit.
PROSITE	PS51208. AUTOTRANSPORTER. 1 hit. PS01098. LIPASE_GDSL_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIP1_PHOLU

Accession

Primary (citable) accession number: P40601

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	July 28, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)