P40600 (LIPE_AERHY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Extracellular lipase
EC=3.1.1.3

Alternative name(s):
Triacylglycerol lipase

Gene names

Name:

lip

Organism

Aeromonas hydrophila

Taxonomic identifier

644 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	684 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The optimum chain lengths for the acyl moiety is C6 for ester hydrolysis and C6 and C8 for triacylglycerol hydrolysis.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subunit structure	Monomer.
Subcellular location	Secreted.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.
Biophysicochemical properties	Temperature dependence: Optimum temperature is 37 degrees Celsius. Retains 90% of its activity after 30 minutes at 55 degrees Celsius.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 48	48	Potential
	Chain	49 – 684	636	Extracellular lipase		PRO_0000017729
Sites
	Active site	568	1	Charge relay system By similarity

Sequences

	Sequence		Length	Mass (Da)
	P40600 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 325418D3936FA428	FASTA	684	71,904
	10 20 30 40 50 60 MKKKLIYAAV VSALLAGCGG SDDNKGDTSS YLDYLLTGSN AVGPSALAAR AWDGTLKFST 70 80 90 100 110 120 ETADLSNPVS AMSTLDGWST TQAIQIVPVT SSGITVQAPT TAEFGASVAP LYLLEVTFDS 130 140 150 160 170 180 TALRPSGVKK VLTYGVDFVV AASAWQAEPG SAQAVEPLPC LANDSGHRTA ERQSRRCLKA 190 200 210 220 230 240 GSDYGNYKNN AGSNAQEQTI NGLIALQEGL FKAATGIATD HVIFSDWFGT QSGADVLVAV 250 260 270 280 290 300 KGAAASVLKA DPVTLDAAKL WKQDAWEHQP ARHLYPGRDR PTCLPDPAGC RAVPAAEQKD 310 320 330 340 350 360 AIATAFGPVL RSTRLLKRPR SIPVPSSCLT SSPHRRPQVP GARPRPSPGT VPSQPVRHRQ 370 380 390 400 410 420 CAEGVTRSDR RAGGGGRGSG PAGDADCRSD PPERAAGRGE QADWGDAHLR RQAAGRRAEH 430 440 450 460 470 480 WSLQPAADAG RGAIRADACL RQGCPQHHHG CHHLSARRDL GQRERLRPGA GPDLEDLCRH 490 500 510 520 530 540 AGGQEGGAGG DRSSAARRAW LRLSGSMDTV TTSDNPTPYL NLSYLTVARD NLKQSVAICW 550 560 570 580 590 600 ACVWRLAWPT PRAIGTAGSL KVHFLGHSLG ASRVPTCCGR QPDHRQRASG CPVQVRYRWP 610 620 630 640 650 660 GHAGSHSAAA AELADFGPTI KMGVLTSGSA ELKAGFTAYA PNCTDGGAYL LRQRVPAEPG 670 680 RGHSATAATR CRVQLCGPVG AGFG

References

[1]

"Purification, gene cloning, amino acid sequence analysis, and expression of an extracellular lipase from an Aeromonas hydrophila human isolate." Anguita J., Rodriguez Aparicio L.B., Naharro G. Appl. Environ. Microbiol. 59:2411-2417(1993) [PubMed: 8368830] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: H3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S65123 Genomic DNA. Translation: AAB28083.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIPE_AERHY

Accession

Primary (citable) accession number: P40600

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 31, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program