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Protein

Protein Wnt-4

Gene

Wnt4

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Binds as a ligand to a family of frizzled seven-transmembrane receptors and acts through a cascade of genes on the nucleus. Acts downstream of homeotic complex genes in the visceral mesoderm and is required for embryonic segmentation. Also required for cell movement and FAK regulation during ovarian morphogenesis.1 Publication

GO - Molecular functioni

  • frizzled binding Source: FlyBase
  • receptor binding Source: FlyBase
  • signal transducer activity Source: UniProtKB

GO - Biological processi

  • cell fate commitment Source: GO_Central
  • cell migration Source: UniProtKB
  • establishment of imaginal disc-derived wing hair orientation Source: FlyBase
  • female gonad development Source: UniProtKB
  • motor neuron axon guidance Source: FlyBase
  • neural retina development Source: FlyBase
  • non-canonical Wnt signaling pathway Source: FlyBase
  • regulation of hemocyte proliferation Source: FlyBase
  • retinal ganglion cell axon guidance Source: FlyBase
  • salivary gland morphogenesis Source: FlyBase
  • synaptic target inhibition Source: FlyBase
  • Wnt signaling pathway Source: FlyBase
  • Wnt signaling pathway, planar cell polarity pathway Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein
Biological processWnt signaling pathway

Enzyme and pathway databases

ReactomeiR-DME-3238698. WNT ligand biogenesis and trafficking.
SignaLinkiP40589.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wnt-4
Alternative name(s):
dWnt-4
Gene namesi
Name:Wnt4
Synonyms:Wnt-4
ORF Names:CG4698
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0010453. Wnt4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000004147822 – 539Protein Wnt-4Add BLAST518

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi74N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi274 ↔ 285By similarity
Glycosylationi284N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi322 ↔ 330By similarity
Disulfide bondi332 ↔ 349By similarity
Disulfide bondi397 ↔ 411By similarity
Disulfide bondi399 ↔ 406By similarity
Lipidationi403O-palmitoleoyl serine; by PORCNBy similarity1
Glycosylationi419N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi486 ↔ 497By similarity
Disulfide bondi512 ↔ 529By similarity
Disulfide bondi514 ↔ 526By similarity
Disulfide bondi521 ↔ 522By similarity

Post-translational modificationi

Palmitoleoylated by porcupine. The lipid group functions as a sorting signal, targeting the ligand to polarized vesicles that transport Wnt4 to unique sites at the cell surface. Depalmitoleoylated by notum, leading to inhibit Wnt signaling pathway.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

Proteomic databases

PaxDbiP40589.
PRIDEiP40589.

Expressioni

Gene expression databases

BgeeiFBgn0010453.
ExpressionAtlasiP40589. differential.
GenevisibleiP40589. DM.

Interactioni

GO - Molecular functioni

  • frizzled binding Source: FlyBase
  • receptor binding Source: FlyBase

Protein-protein interaction databases

BioGridi60153. 11 interactors.
IntActiP40589. 3 interactors.
MINTiMINT-8291179.
STRINGi7227.FBpp0088345.

Structurei

3D structure databases

ProteinModelPortaliP40589.
SMRiP40589.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3913. Eukaryota.
ENOG410XQZ1. LUCA.
GeneTreeiENSGT00690000101857.
InParanoidiP40589.
OMAiYNEHFIS.
OrthoDBiEOG091G0OFF.
PhylomeDBiP40589.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR015943. WD40/YVTN_repeat-like_dom_sf.
IPR005817. Wnt.
IPR018161. Wnt_CS.
PANTHERiPTHR12027. PTHR12027. 1 hit.
PfamiView protein in Pfam
PF00110. wnt. 1 hit.
PRINTSiPR01349. WNTPROTEIN.
SMARTiView protein in SMART
SM00097. WNT1. 1 hit.
PROSITEiView protein in PROSITE
PS00246. WNT1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40589-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSPTGVFVL MILTHLSFGL GQVRNEDQLL MVGQNGDLDS SNPAIHHQQH
60 70 80 90 100
QQHQQHQQHQ QHQSNHNLNN GNMNSTILNT LMGNNAGQVV NSSPGGGGSM
110 120 130 140 150
INQLGSSTSS VPSVIGGGVG SVGNPWHSAV GLGVPGNGMG LPSSHGLGGN
160 170 180 190 200
MGSHPHGHAL AGLAKLGIIV PGGQGLPGNL GYGGTMLNGG GVGGAAGMGL
210 220 230 240 250
GIGSNTNNMD MQQGLYNEHF ISEHTVMAVF TSQGQVGGPC RYMPATRRQN
260 270 280 290 300
HQCRKETGLP GTLSEARRLA TTHCEEQFRY DRWNCSIETR GKRNIFKKLY
310 320 330 340 350
KETAFVHALT AAAMTHSIAR ACAEGRMTKC SCGPKKHNRE AQDFQWGGCN
360 370 380 390 400
DNLKHGKRVT RSFLDLRGGD GDEVSEILRH DSEVGIEAVS SQMMDKCKCH
410 420 430 440 450
GVSGSCSMKT CWKKMADFNA TATLLRQKYN EAIRKAPNQR SMRQVSSSRM
460 470 480 490 500
KKPKQRRKKP QQSQYTTLYY LETSPSYCAV TKDRQCLHPD NCGTLCCGRG
510 520 530
YTTQVVKQVE KCRCRFNNGR CCQLICDYCQ RLENKYFCK
Length:539
Mass (Da):58,685
Last modified:January 27, 2003 - v2
Checksum:i6682C8B3D729D067
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18F → L in AAN04479 (PubMed:11970894).Curated1
Sequence conflicti201G → R in AAN04479 (PubMed:11970894).Curated1
Sequence conflicti230F → L in AAM11406 (PubMed:12537569).Curated1
Sequence conflicti434R → A in AAN04479 (PubMed:11970894).Curated1
Sequence conflicti532L → V in AAN04479 (PubMed:11970894).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF533773 mRNA. Translation: AAN04479.1.
AE014134 Genomic DNA. Translation: AAF52499.2.
AY095078 mRNA. Translation: AAM11406.1.
RefSeqiNP_001260187.1. NM_001273258.2.
NP_476972.2. NM_057624.4.
UniGeneiDm.341.

Genome annotation databases

EnsemblMetazoaiFBtr0089291; FBpp0088345; FBgn0010453.
FBtr0334799; FBpp0306845; FBgn0010453.
GeneIDi34007.
KEGGidme:Dmel_CG4698.

Similar proteinsi

Entry informationi

Entry nameiWNT4_DROME
AccessioniPrimary (citable) accession number: P40589
Secondary accession number(s): Q8MPQ1, Q8SWU3, Q9VM29
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 27, 2003
Last modified: November 22, 2017
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families