P40582 (GST1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase 1
EC=2.5.1.18

Alternative name(s):
GST-I

Gene names

Name:	GTT1
Ordered Locus Names:	YIR038C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the GST superfamily. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutathione metabolic process Inferred from direct assay Ref.3. Source: SGD
Cellular_component	endoplasmic reticulum Inferred from direct assay Ref.3. Source: SGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from direct assay PubMed 16407407. Source: SGD plasma membrane Inferred from direct assay PubMed 16622836. Source: SGD
Molecular_function	glutathione peroxidase activity Inferred from direct assay PubMed 16709151. Source: SGD glutathione transferase activity Inferred from direct assay Ref.3. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 234	234	Glutathione S-transferase 1		PRO_0000185987
Regions
	Domain	3 – 90	88	GST N-terminal
	Domain	96 – 234	139	GST C-terminal
Amino acid modifications
	Modified residue	56	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P40582 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 3C07D40D29F60DFF	FASTA	234	26,795
	10 20 30 40 50 60 MSLPIIKVHW LDHSRAFRLL WLLDHLNLEY EIVPYKRDAN FRAPPELKKI HPLGRSPLLE 70 80 90 100 110 120 VQDRETGKKK ILAESGFIFQ YVLQHFDHSH VLMSEDADIA DQINYYLFYV EGSLQPPLMI 130 140 150 160 170 180 EFILSKVKDS GMPFPISYLA RKVADKISQA YSSGEVKNQF DFVEGEISKN NGYLVDGKLS 190 200 210 220 230 GADILMSFPL QMAFERKFAA PEDYPAISKW LKTITSEESY AASKEKARAL GSNF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"A novel membrane-bound glutathione S-transferase functions in the stationary phase of the yeast Saccharomyces cerevisiae." Choi J.H., Lou W., Vancura A. J. Biol. Chem. 273:29915-29922(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z38061 Genomic DNA. Translation: CAA86198.1. BK006942 Genomic DNA. Translation: DAA08585.1.
PIR	S48500.
RefSeq	NP_012304.1. NM_001179560.1.
3D structure databases
ProteinModelPortal	P40582.
SMR	P40582. Positions 5-226.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2103N.
IntAct	P40582. 64 interactions.
MINT	MINT-566298.
STRING	4932.YIR038C.
Proteomic databases
PaxDb	P40582.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIR038C; YIR038C; YIR038C.
GeneID	854856.
KEGG	sce:YIR038C.
Organism-specific databases
CYGD	YIR038c.
SGD	S000001477. GTT1.
Phylogenomic databases
eggNOG	COG0625.
HOGENOM	HOG000125753.
KO	K00799.
OMA	ADVQMSF.
OrthoDB	EOG4GMZ6F.
Enzyme and pathway databases
SABIO-RK	P40582.
Gene expression databases
Genevestigator	P40582.
GermOnline	YIR038C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977767.

Entry information

Entry name

GST1_YEAST

Accession

Primary (citable) accession number: P40582
Secondary accession number(s): D6VVW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program