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Protein

Peroxiredoxin HYR1

Gene

HYR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in oxidative stress response and redox homeostasis. Functions as a sensor and transducer of hydroperoxide stress. In response to hydroperoxide stress it oxidizes (activates) the transcription activator YAP1, which is involved in transcription activation of genes of the oxidative stress response pathway. May also play a direct role in hydroperoxide scavenging, being the most active of three closely related S.cerevisiae peroxiredoxins (GPX1, GPX2, and HYP1/GPX3) with respect to peroxide and lipid hydroperoxide reduction. The three enzymes are not required for the glutaredoxin-mediated antioxidant function. In the presence of peroxides, HYP1 is directly oxidized at Cys-36 to form a cysteine sulfenic acid (-SOH). Cys-36-SOH then forms either an intramolecular disulfide bond (Cys-36 with Cys-82) or a transient, intermolecular disulfide bond with 'Cys-598' of YAP1, which is further resolved into a YAP1 intramolecular disulfide bond ('Cys-303' with 'Cys-598') and a reduced Cys-36 in HYP1/GPX3.8 Publications

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei36Cysteine sulfenic acid (-SOH) intermediate1

GO - Molecular functioni

  • glutathione peroxidase activity Source: SGD
  • peroxiredoxin activity Source: UniProtKB-EC
  • phospholipid-hydroperoxide glutathione peroxidase activity Source: SGD

GO - Biological processi

  • cellular response to oxidative stress Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Enzyme and pathway databases

BioCyciYEAST:YIR037W-MONOMER.
ReactomeiR-SCE-2142712. Synthesis of 12-eicosatetraenoic acid derivatives.
R-SCE-2142770. Synthesis of 15-eicosatetraenoic acid derivatives.
R-SCE-3299685. Detoxification of Reactive Oxygen Species.
R-SCE-5628897. TP53 Regulates Metabolic Genes.
R-SCE-74259. Purine catabolism.

Protein family/group databases

PeroxiBasei3742. SceGPx03.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxiredoxin HYR1 (EC:1.11.1.15)
Alternative name(s):
Glutathione peroxidase 3
Hydrogen peroxide resistance protein 1
Oxidant receptor peroxidase 1
Phospholipid hydroperoxide glutathione peroxidase 3
Short name:
PHGPx3
Gene namesi
Name:HYR1
Synonyms:GPX3, ORP1
Ordered Locus Names:YIR037W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIR037W.
SGDiS000001476. HYR1.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: SGD
  • mitochondrial intermembrane space Source: SGD
  • peroxisomal matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi82C → S: Loss of enzyme activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000666431 – 163Peroxiredoxin HYR1Add BLAST163

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 82Redox-active
Disulfide bondi36Interchain (with C-598 in YAP1); transient

Post-translational modificationi

Reversible disulfide bond formation between Cys-36 and Cys-82 upon peroxide reduction; probably reverted by thioredoxin (TRX2).

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP40581.
PRIDEiP40581.
TopDownProteomicsiP40581.

PTM databases

iPTMnetiP40581.

Expressioni

Inductioni

In contrast to the other two peroxiredoxins, HYP1/GPX3 expression is constitutive, not stress-induced.1 Publication

Interactioni

Subunit structurei

Interacts with YAP1 and probably YBP1.1 Publication

Protein-protein interaction databases

BioGridi35028. 43 interactors.
DIPiDIP-1275N.
IntActiP40581. 14 interactors.
MINTiMINT-401428.

Structurei

Secondary structure

1163
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 6Combined sources4
Helixi19 – 22Combined sources4
Beta strandi26 – 36Combined sources11
Helixi39 – 52Combined sources14
Helixi53 – 55Combined sources3
Beta strandi57 – 64Combined sources8
Beta strandi97 – 100Combined sources4
Helixi105 – 113Combined sources9
Beta strandi117 – 119Combined sources3
Beta strandi129 – 132Combined sources4
Beta strandi134 – 136Combined sources3
Beta strandi138 – 142Combined sources5
Helixi148 – 151Combined sources4
Helixi152 – 159Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CMIX-ray2.02A1-163[»]
ProteinModelPortaliP40581.
SMRiP40581.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40581.

Family & Domainsi

Sequence similaritiesi

Belongs to the glutathione peroxidase family.Curated

Keywords - Domaini

Redox-active center

Phylogenomic databases

GeneTreeiENSGT00760000119230.
HOGENOMiHOG000277054.
InParanoidiP40581.
KOiK00432.
OMAiIASFCET.
OrthoDBiEOG092C4H0P.

Family and domain databases

CDDicd00340. GSH_Peroxidase. 1 hit.
Gene3Di3.40.30.10. 1 hit.
InterProiIPR000889. Glutathione_peroxidase.
IPR029759. GPX_AS.
IPR029760. GPX_CS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11592. PTHR11592. 1 hit.
PfamiPF00255. GSHPx. 1 hit.
[Graphical view]
PIRSFiPIRSF000303. Glutathion_perox. 1 hit.
PRINTSiPR01011. GLUTPROXDASE.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00460. GLUTATHIONE_PEROXID_1. 1 hit.
PS00763. GLUTATHIONE_PEROXID_2. 1 hit.
PS51355. GLUTATHIONE_PEROXID_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40581-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEFYKLAPV DKKGQPFPFD QLKGKVVLIV NVASKCGFTP QYKELEALYK
60 70 80 90 100
RYKDEGFTII GFPCNQFGHQ EPGSDEEIAQ FCQLNYGVTF PIMKKIDVNG
110 120 130 140 150
GNEDPVYKFL KSQKSGMLGL RGIKWNFEKF LVDKKGKVYE RYSSLTKPSS
160
LSETIEELLK EVE
Length:163
Mass (Da):18,641
Last modified:February 1, 1995 - v1
Checksum:i46C42B81E895C1A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22446 Genomic DNA. Translation: AAA64283.1.
Z38061 Genomic DNA. Translation: CAA86197.1.
BK006942 Genomic DNA. Translation: DAA08584.1.
PIRiS48499.
RefSeqiNP_012303.1. NM_001179559.1.

Genome annotation databases

EnsemblFungiiYIR037W; YIR037W; YIR037W.
GeneIDi854855.
KEGGisce:YIR037W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22446 Genomic DNA. Translation: AAA64283.1.
Z38061 Genomic DNA. Translation: CAA86197.1.
BK006942 Genomic DNA. Translation: DAA08584.1.
PIRiS48499.
RefSeqiNP_012303.1. NM_001179559.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CMIX-ray2.02A1-163[»]
ProteinModelPortaliP40581.
SMRiP40581.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35028. 43 interactors.
DIPiDIP-1275N.
IntActiP40581. 14 interactors.
MINTiMINT-401428.

Protein family/group databases

PeroxiBasei3742. SceGPx03.

PTM databases

iPTMnetiP40581.

Proteomic databases

MaxQBiP40581.
PRIDEiP40581.
TopDownProteomicsiP40581.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIR037W; YIR037W; YIR037W.
GeneIDi854855.
KEGGisce:YIR037W.

Organism-specific databases

EuPathDBiFungiDB:YIR037W.
SGDiS000001476. HYR1.

Phylogenomic databases

GeneTreeiENSGT00760000119230.
HOGENOMiHOG000277054.
InParanoidiP40581.
KOiK00432.
OMAiIASFCET.
OrthoDBiEOG092C4H0P.

Enzyme and pathway databases

BioCyciYEAST:YIR037W-MONOMER.
ReactomeiR-SCE-2142712. Synthesis of 12-eicosatetraenoic acid derivatives.
R-SCE-2142770. Synthesis of 15-eicosatetraenoic acid derivatives.
R-SCE-3299685. Detoxification of Reactive Oxygen Species.
R-SCE-5628897. TP53 Regulates Metabolic Genes.
R-SCE-74259. Purine catabolism.

Miscellaneous databases

EvolutionaryTraceiP40581.
PROiP40581.

Family and domain databases

CDDicd00340. GSH_Peroxidase. 1 hit.
Gene3Di3.40.30.10. 1 hit.
InterProiIPR000889. Glutathione_peroxidase.
IPR029759. GPX_AS.
IPR029760. GPX_CS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11592. PTHR11592. 1 hit.
PfamiPF00255. GSHPx. 1 hit.
[Graphical view]
PIRSFiPIRSF000303. Glutathion_perox. 1 hit.
PRINTSiPR01011. GLUTPROXDASE.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00460. GLUTATHIONE_PEROXID_1. 1 hit.
PS00763. GLUTATHIONE_PEROXID_2. 1 hit.
PS51355. GLUTATHIONE_PEROXID_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPX3_YEAST
AccessioniPrimary (citable) accession number: P40581
Secondary accession number(s): D6VVW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8000 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally thought to be a glutathione peroxidase or a phospholipid hydroperoxide glutathione peroxidase. HYP1 does not require glutathione for hydroperoxide reduction.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.