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Protein

Benzil reductase ((S)-benzoin forming) IRC24

Gene

IRC24

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Reduces benzil stereospecifically to (S)-benzoin. Is probably involved in a pathway contributing to genomic integrity.

Catalytic activityi

(S)-benzoin + NADP+ = benzil + NADPH.1 Publication

Kineticsi

  1. KM=207 µM for benzil1 Publication
  2. KM=29 µM for 1-phenyl-1,2-propanedione1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei144 – 1441SubstrateBy similarity
    Active sitei157 – 1571Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi6 – 3025NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    • oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor Source: SGD
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31450-MONOMER.
    ReactomeiR-SCE-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Benzil reductase ((S)-benzoin forming) IRC24 (EC:1.1.1.320)
    Alternative name(s):
    Increased recombination centers protein 24
    Gene namesi
    Name:IRC24
    Ordered Locus Names:YIR036C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome IX

    Organism-specific databases

    EuPathDBiFungiDB:YIR036C.
    SGDiS000001475. IRC24.

    Pathology & Biotechi

    Disruption phenotypei

    Displays increased levels of spontaneous RAD52 foci in proliferating diploid cells.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 263263Benzil reductase ((S)-benzoin forming) IRC24PRO_0000054871Add
    BLAST

    Proteomic databases

    MaxQBiP40580.

    Expressioni

    Inductioni

    By single-strand DNA damage.1 Publication

    Interactioni

    Protein-protein interaction databases

    BioGridi35027. 9 interactions.
    DIPiDIP-4707N.
    MINTiMINT-523694.

    Structurei

    3D structure databases

    ProteinModelPortaliP40580.
    SMRiP40580. Positions 1-251.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00440000033609.
    InParanoidiP40580.
    OMAiPKNFIAP.
    OrthoDBiEOG092C4ECY.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    IPR002347. SDR_fam.
    [Graphical view]
    PANTHERiPTHR24322. PTHR24322. 2 hits.
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    SUPFAMiSSF51735. SSF51735. 1 hit.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40580-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGKVILITGA SRGIGLQLVK TVIEEDDECI VYGVARTEAG LQSLQREYGA
    60 70 80 90 100
    DKFVYRVLDI TDRSRMEALV EEIRQKHGKL DGIVANAGML EPVKSISQSN
    110 120 130 140 150
    SEHDIKQWER LFDVNFFSIV SLVALCLPLL KSSPFVGNIV FVSSGASVKP
    160 170 180 190 200
    YNGWSAYGCS KAALNHFAMD IASEEPSDKV RAVCIAPGVV DTQMQKDIRE
    210 220 230 240 250
    TLGPQGMTPK ALERFTQLYK TSSLLDPKVP AAVLAQLVLK GIPDSLNGQY
    260
    LRYNDERLGP VQG
    Length:263
    Mass (Da):28,804
    Last modified:February 1, 1995 - v1
    Checksum:i53DE25425AAC946F
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z38061 Genomic DNA. Translation: CAA86196.1.
    AY558240 Genomic DNA. Translation: AAS56566.1.
    AB052924 Genomic DNA. Translation: BAB86002.1.
    BK006942 Genomic DNA. Translation: DAA08583.1.
    PIRiS48498.
    RefSeqiNP_012302.3. NM_001179558.3.

    Genome annotation databases

    EnsemblFungiiYIR036C; YIR036C; YIR036C.
    GeneIDi854854.
    KEGGisce:YIR036C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z38061 Genomic DNA. Translation: CAA86196.1.
    AY558240 Genomic DNA. Translation: AAS56566.1.
    AB052924 Genomic DNA. Translation: BAB86002.1.
    BK006942 Genomic DNA. Translation: DAA08583.1.
    PIRiS48498.
    RefSeqiNP_012302.3. NM_001179558.3.

    3D structure databases

    ProteinModelPortaliP40580.
    SMRiP40580. Positions 1-251.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi35027. 9 interactions.
    DIPiDIP-4707N.
    MINTiMINT-523694.

    Proteomic databases

    MaxQBiP40580.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYIR036C; YIR036C; YIR036C.
    GeneIDi854854.
    KEGGisce:YIR036C.

    Organism-specific databases

    EuPathDBiFungiDB:YIR036C.
    SGDiS000001475. IRC24.

    Phylogenomic databases

    GeneTreeiENSGT00440000033609.
    InParanoidiP40580.
    OMAiPKNFIAP.
    OrthoDBiEOG092C4ECY.

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31450-MONOMER.
    ReactomeiR-SCE-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.

    Miscellaneous databases

    PROiP40580.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    IPR002347. SDR_fam.
    [Graphical view]
    PANTHERiPTHR24322. PTHR24322. 2 hits.
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    SUPFAMiSSF51735. SSF51735. 1 hit.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiBZRD_YEAST
    AccessioniPrimary (citable) accession number: P40580
    Secondary accession number(s): D6VVW7, Q54AB5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: September 7, 2016
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1620 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.