ID   YIV5_YEAST              Reviewed;         254 AA.
AC   P40579; D6VVW6;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   27-MAR-2024, entry version 169.
DE   RecName: Full=Uncharacterized oxidoreductase YIR035C;
DE            EC=1.-.-.-;
GN   OrderedLocusNames=YIR035C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169870;
RA   Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA   Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA   Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA   Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA   Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL   Nature 387:84-87(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [5] {ECO:0007744|PDB:3KZV}
RP   X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS).
RA   Zhang R., Xu X., Cui H., Savchenko A., Edwards A., Joachimiak A.;
RT   "The crystal structure of a cytoplasmic protein with unknown function from
RT   Saccharomyces cerevisiae.";
RL   Submitted (DEC-2009) to the PDB data bank.
RN   [6] {ECO:0007744|PDB:6UHX}
RP   X-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS).
RA   Chen C.;
RT   "Crystal structure of YIR035C short chain dehydrogenases/reductase from
RT   Saccharomyces cerevisiae.";
RL   Submitted (SEP-2019) to the PDB data bank.
CC   -!- MISCELLANEOUS: Present with 2370 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; Z38061; CAA86195.1; -; Genomic_DNA.
DR   EMBL; AY692591; AAT92610.1; -; Genomic_DNA.
DR   EMBL; BK006942; DAA08582.1; -; Genomic_DNA.
DR   PIR; S48497; S48497.
DR   RefSeq; NP_012301.3; NM_001179557.3.
DR   PDB; 3KZV; X-ray; 2.00 A; A=1-254.
DR   PDB; 6UHX; X-ray; 2.75 A; A/B=1-254.
DR   PDBsum; 3KZV; -.
DR   PDBsum; 6UHX; -.
DR   AlphaFoldDB; P40579; -.
DR   SMR; P40579; -.
DR   BioGRID; 35026; 52.
DR   DIP; DIP-4612N; -.
DR   IntAct; P40579; 3.
DR   MINT; P40579; -.
DR   STRING; 4932.YIR035C; -.
DR   iPTMnet; P40579; -.
DR   MaxQB; P40579; -.
DR   PaxDb; 4932-YIR035C; -.
DR   PeptideAtlas; P40579; -.
DR   EnsemblFungi; YIR035C_mRNA; YIR035C; YIR035C.
DR   GeneID; 854853; -.
DR   KEGG; sce:YIR035C; -.
DR   AGR; SGD:S000001474; -.
DR   SGD; S000001474; YIR035C.
DR   VEuPathDB; FungiDB:YIR035C; -.
DR   eggNOG; KOG1204; Eukaryota.
DR   GeneTree; ENSGT00940000176436; -.
DR   HOGENOM; CLU_010194_2_11_1; -.
DR   InParanoid; P40579; -.
DR   OMA; SHVDEWR; -.
DR   OrthoDB; 2785417at2759; -.
DR   BioCyc; YEAST:G3O-31449-MONOMER; -.
DR   BioGRID-ORCS; 854853; 0 hits in 10 CRISPR screens.
DR   EvolutionaryTrace; P40579; -.
DR   PRO; PR:P40579; -.
DR   Proteomes; UP000002311; Chromosome IX.
DR   RNAct; P40579; Protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0050664; F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor; IBA:GO_Central.
DR   CDD; cd05367; SPR-like_SDR_c; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   PANTHER; PTHR43008; BENZIL REDUCTASE; 1.
DR   PANTHER; PTHR43008:SF8; BENZIL REDUCTASE ((S)-BENZOIN FORMING) IRC24; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   1: Evidence at protein level;
KW   3D-structure; NADP; Oxidoreductase; Reference proteome.
FT   CHAIN           1..254
FT                   /note="Uncharacterized oxidoreductase YIR035C"
FT                   /id="PRO_0000054870"
FT   ACT_SITE        136
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:O93868"
FT   ACT_SITE        150
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   ACT_SITE        154
FT                   /note="Lowers pKa of active site Tyr"
FT                   /evidence="ECO:0000250|UniProtKB:O93868"
FT   BINDING         7
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:L0E2Z4"
FT   BINDING         85
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:O93868"
FT   BINDING         150
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:O93868"
FT   BINDING         154
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:O93868"
FT   BINDING         181
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:O93868"
FT   BINDING         183
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:L0E2Z4"
FT   STRAND          4..7
FT                   /evidence="ECO:0007829|PDB:3KZV"
FT   HELIX           13..25
FT                   /evidence="ECO:0007829|PDB:3KZV"
FT   STRAND          30..36
FT                   /evidence="ECO:0007829|PDB:3KZV"
FT   HELIX           38..48
FT                   /evidence="ECO:0007829|PDB:3KZV"
FT   HELIX           49..51
FT                   /evidence="ECO:0007829|PDB:3KZV"
FT   STRAND          52..57
FT                   /evidence="ECO:0007829|PDB:3KZV"
FT   HELIX           62..76
FT                   /evidence="ECO:0007829|PDB:3KZV"
FT   STRAND          81..85
FT                   /evidence="ECO:0007829|PDB:3KZV"
FT   TURN            93..96
FT                   /evidence="ECO:0007829|PDB:3KZV"
FT   HELIX           100..110
FT                   /evidence="ECO:0007829|PDB:3KZV"
FT   HELIX           112..128
FT                   /evidence="ECO:0007829|PDB:3KZV"
FT   STRAND          131..135
FT                   /evidence="ECO:0007829|PDB:3KZV"
FT   HELIX           138..140
FT                   /evidence="ECO:0007829|PDB:6UHX"
FT   HELIX           148..155
FT                   /evidence="ECO:0007829|PDB:3KZV"
FT   HELIX           157..167
FT                   /evidence="ECO:0007829|PDB:3KZV"
FT   STRAND          171..177
FT                   /evidence="ECO:0007829|PDB:3KZV"
FT   HELIX           184..191
FT                   /evidence="ECO:0007829|PDB:6UHX"
FT   TURN            195..197
FT                   /evidence="ECO:0007829|PDB:3KZV"
FT   HELIX           200..210
FT                   /evidence="ECO:0007829|PDB:3KZV"
FT   HELIX           219..231
FT                   /evidence="ECO:0007829|PDB:3KZV"
FT   HELIX           235..237
FT                   /evidence="ECO:0007829|PDB:3KZV"
FT   STRAND          241..243
FT                   /evidence="ECO:0007829|PDB:3KZV"
FT   HELIX           247..252
FT                   /evidence="ECO:0007829|PDB:3KZV"
SQ   SEQUENCE   254 AA;  27481 MW;  916A5A460BE59F78 CRC64;
     MGKVILVTGV SRGIGKSIVD VLFSLDKDTV VYGVARSEAP LKKLKEKYGD RFFYVVGDIT
     EDSVLKQLVN AAVKGHGKID SLVANAGVLE PVQNVNEIDV NAWKKLYDIN FFSIVSLVGI
     ALPELKKTNG NVVFVSSDAC NMYFSSWGAY GSSKAALNHF AMTLANEERQ VKAIAVAPGI
     VDTDMQVNIR ENVGPSSMSA EQLKMFRGLK ENNQLLDSSV PATVYAKLAL HGIPDGVNGQ
     YLSYNDPALA DFMP
//