P40577 (MND2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anaphase-promoting complex subunit MND2

Alternative name(s):
Meiotic nuclear division protein 2

Gene names

Name:	MND2
Ordered Locus Names:	YIR025W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	The APC/C is composed of at least 13 subunits that stay tightly associated throughout the cell cycle: APC1, APC2, APC4, APC5, APC9, APC11, CDC16, CDC23, CDC26, CDC27, DOC1, MND2 and SWM1. MND2 interacts directly with APC1, APC5 and CDC23. Ref.3 Ref.4
Sequence similarities	Belongs to the APC15 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Ubl conjugation pathway
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from physical interaction Ref.4. Source: SGD cell division Inferred from electronic annotation. Source: UniProtKB-KW mitotic sister chromatid segregation Inferred from mutant phenotype. Source: SGD protein ubiquitination Inferred from physical interaction Ref.4. Source: SGD reciprocal meiotic recombination Inferred from mutant phenotype. Source: SGD regulation of mitotic metaphase/anaphase transition Inferred from mutant phenotype Ref.4. Source: SGD
Cellular component	anaphase-promoting complex Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction Ref.4. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APC1	P53886	3	EBI-25433,EBI-29017
APC2	Q12440	3	EBI-25433,EBI-33503
APC4	Q04601	5	EBI-25433,EBI-32842
APC5	Q08683	4	EBI-25433,EBI-35371
CDC23	P16522	3	EBI-25433,EBI-4216

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	Anaphase-promoting complex subunit MND2		PRO_0000096523

Sequences

	Sequence		Length	Mass (Da)
	P40577 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: D41276561E6ED509	FASTA	368	42,826
	10 20 30 40 50 60 MARALRDISL FNDIRKDQNS AGAKHERYNM RDLRSKKNQH VNGIDDYEDD SLDRFIRRKK 70 80 90 100 110 120 SRVVKYIPSL SAYNVFNEFP YYPTSASQLL DGKLDEFLML SEQYKSRLPK IRKLGWNRFK 130 140 150 160 170 180 PIGINKTMYE LEMLRSRARA QNAEGNNEED FRQHDSREED PRNNGSIGRV ILPHILQENE 190 200 210 220 230 240 EYDTGEGVTG LHSMPNDSMA ILANNSANNS QNEEVSEEDE ISYDYDAEFD HVVDEDDNEE 250 260 270 280 290 300 GEVPGEGVEG IEVQRERIVP DDLLMRPTSL SRSLQQFVEE AHHLDRNPYD IDSDNDGEDS 310 320 330 340 350 360 KVELDMNPDF EDDVGREHDY NSEYSQEPTS YGGITPDLAS NWRNWTRERI TSLDELMERR ARQQRGQD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed: 9169870] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes." Yoon H.-J., Feoktistova A., Wolfe B.A., Jennings J.L., Link A.J., Gould K.L. Curr. Biol. 12:2048-2054(2002) [PubMed: 12477395] [Abstract] Cited for: SUBUNIT.
[4]	"Mnd2 and Swm1 are core subunits of the Saccharomyces cerevisiae anaphase-promoting complex." Hall M.C., Torres M.P., Schroeder G.K., Borchers C.H. J. Biol. Chem. 278:16698-16705(2003) [PubMed: 12609981] [Abstract] Cited for: SUBUNIT, INTERACTION WITH APC1; APC5 AND CDC23.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z38061 Genomic DNA. Translation: CAA86185.1. BK006942 Genomic DNA. Translation: DAA08572.1.
PIR	S48487.
RefSeq	NP_012291.1. NM_001179547.1.
3D structure databases
ProteinModelPortal	P40577.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1536N.
IntAct	P40577. 16 interactions.
MINT	MINT-411309.
STRING	P40577.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIR025W; YIR025W; YIR025W.
GeneID	854843.
KEGG	sce:YIR025W.
NMPDR	fig|4932.3.peg.1834.
Organism-specific databases
CYGD	YIR025w.
SGD	S000001464. MND2.
Phylogenomic databases
eggNOG	fuNOG13656.
OMA	ISYDYDA.
OrthoDB	EOG4SR169.
Gene expression databases
ArrayExpress	P40577.
Genevestigator	P40577.
GermOnline	YIR025W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR008402. APC_su15/MND2. IPR016807. APC_suMND2. [Graphical view]
KO	K12781.
Pfam	PF05841. Apc15p. 1 hit. [Graphical view]
PIRSF	PIRSF022699. MND2. 1 hit.
ProtoNet	Search...
Other
NextBio	977728.

Entry information

Entry name

MND2_YEAST

Accession

Primary (citable) accession number: P40577
Secondary accession number(s): D6VVV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program