P40571 (RPR2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease P protein subunit RPR2
EC=3.1.26.5

Alternative name(s):
RNA-processing protein RPR2
RNase P 16.4 kDa subunit

Gene names

Name:	RPR2
Ordered Locus Names:	YIR015W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	144 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Ref.4
Catalytic activity	Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.
Subunit structure	Component of nuclear RNase P. RNase P consists of an RNA moiety and at least 9 protein subunits including POP1, POP3, POP4, POP5, POP6, POP7, POP8, RPP1 and RPR2, many of which are shared with the RNase MPR complex. Ref.4
Subcellular location	Nucleus Ref.5.
Miscellaneous	Present with 2070 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the eukaryotic/archaeal RNase P protein component 4 family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intronic box C/D snoRNA processing Inferred from direct assay PubMed 18713869. Source: SGD tRNA processing Traceable author statement Ref.4. Source: SGD
Cellular_component	nucleolar ribonuclease P complex Inferred from direct assay PubMed 19095620Ref.4. Source: SGD
Molecular_function	ribonuclease P activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 144	144	Ribonuclease P protein subunit RPR2		PRO_0000153847

Sequences

	Sequence		Length	Mass (Da)
	P40571 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 91AEF14F80D76148	FASTA	144	16,344
	10 20 30 40 50 60 MGKKAHGGKM KPEIDENGTL LVPPPRTIAN QDHFHRLNYL YQISAYQTRA RQKARTDAHT 70 80 90 100 110 120 PLARNYIKSM DLISKKTKTS LLPTIKRTIC KKCHRLLWTP KKLEITSDGA LSVMCGCGTV 130 140 KRFNIGADPN YRTYSEREGN LLNS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP." Chamberlain J.R., Lee Y., Lane W.S., Engelke D.R. Genes Dev. 12:1678-1690(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE RNASE P COMPLEX BY MASS SPECTROMETRY.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z37996 Genomic DNA. Translation: CAA86085.1. AY557849 Genomic DNA. Translation: AAS56175.1. BK006942 Genomic DNA. Translation: DAA08561.1.
PIR	S48359.
RefSeq	NP_012280.1. NM_001179537.1.
3D structure databases
ProteinModelPortal	P40571.
SMR	P40571. Positions 35-127.
ModBase	Search...
Protein-protein interaction databases
IntAct	P40571. 3 interactions.
MINT	MINT-2786613.
STRING	4932.YIR015W.
Proteomic databases
PaxDb	P40571.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIR015W; YIR015W; YIR015W.
GeneID	854832.
KEGG	sce:YIR015W.
Organism-specific databases
CYGD	YIR015w.
SGD	S000001454. RPR2.
Phylogenomic databases
eggNOG	COG2023.
HOGENOM	HOG000231207.
KO	K03540.
OMA	ARISYLY.
OrthoDB	EOG4936TK.
Gene expression databases
Genevestigator	P40571.
GermOnline	YIR015W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007175. Rpr2. [Graphical view]
PANTHER	PTHR14742. PTHR14742. 1 hit.
Pfam	PF04032. Rpr2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977701.

Entry information

Entry name

RPR2_YEAST

Accession

Primary (citable) accession number: P40571
Secondary accession number(s): D6VVU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program