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Protein

Kinetochore-associated protein DSN1

Gene

DSN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as essential component of the kinetochore MIND complex, which is required for the spindle checkpoint and kinetochore integrity. MIND plays a role in establishing a bipolar spindle-kinetochore interaction by joining kinetochore subunits contacting DNA to those contacting microtubules.2 Publications

Miscellaneous

Present with 1310 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • attachment of spindle microtubules to kinetochore involved in homologous chromosome segregation Source: SGD
  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: SGD

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-31431-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore-associated protein DSN1
Gene namesi
Name:DSN1
Ordered Locus Names:YIR010W
ORF Names:YIB10W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIR010W.
SGDiS000001449. DSN1.

Subcellular locationi

GO - Cellular componenti

  • kinetochore Source: SGD
  • nuclear MIS12/MIND complex Source: SGD
  • spindle pole Source: SGD

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000800241 – 576Kinetochore-associated protein DSN1Add BLAST576

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei250PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40568.
PRIDEiP40568.

PTM databases

iPTMnetiP40568.

Interactioni

Subunit structurei

Component of the MIND kinetochore complex, which is composed of at least MTW1, NNF1, NSL1 and DSN1.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35002. 639 interactors.
DIPiDIP-4794N.
IntActiP40568. 18 interactors.
MINTiMINT-546805.

Structurei

Secondary structure

1576
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi561 – 570Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5T6JX-ray1.75C560-572[»]
ProteinModelPortaliP40568.
SMRiP40568.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000112286.
InParanoidiP40568.
KOiK11560.
OMAiIISPHKD.
OrthoDBiEOG092C5AKJ.

Family and domain databases

InterProiView protein in InterPro
IPR013218. Dsn1/Mis13.
IPR018247. EF_Hand_1_Ca_BS.
PANTHERiPTHR14778. PTHR14778. 1 hit.
PfamiView protein in Pfam
PF08202. MIS13. 1 hit.

Sequencei

Sequence statusi: Complete.

P40568-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLEPTQTVS GTPPMLHQRT HKQVYPLRME TIPILESDSK ATLQSNEPTQ
60 70 80 90 100
KDEEETEYFE NKQSVSNLSP DLKFKRHKNK HIQGFPTLGE RLDNLQDIKK
110 120 130 140 150
AKRVENFNSS APIADDNHSG DATANATANA TANATANVNA SAMPAPYMPY
160 170 180 190 200
YYYYHPMNAP TPAMIPYPGS PMHSIMPNSS LQPFYSQPTA AGGPDMTTPQ
210 220 230 240 250
NISSSQQLLP APQLFPYGSF HQQQLQQPHY IQRTRERKKS IGSQRGRRLS
260 270 280 290 300
MLASQANGGS TIISPHKDIP EEDFYTVVGN ASFGKNLQIR QLFNWCLMRS
310 320 330 340 350
LHKLELKAKN QEEEGELEHL TKKSKLESTK AETDYVDPKR LAMVIIKEFV
360 370 380 390 400
DDLKKDHIAI DWEDEEKYED EDEEKILDNT ENYDDTELRQ LFQENDDDDD
410 420 430 440 450
DDDEVDYSEI QRSRRKFSER RKALPKEPKK LLPNSKNVEN TKNLSILTSK
460 470 480 490 500
VNAIKNEVKE WAVTLDTSRP DLEWQELTSF SSQPLEPLSD TEEPDLAIAD
510 520 530 540 550
VETKLETKVD ELRYQSHILN SHSLALNEIT NSKVNKLNIE TMRKISSETD
560 570
DDHSQVINPQ QLLKGLSLSF SKKLDL
Length:576
Mass (Da):65,692
Last modified:February 1, 1995 - v1
Checksum:iF859D3547378C072
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79743 Genomic DNA. No translation available.
Z37996 Genomic DNA. Translation: CAA86080.1.
BK006942 Genomic DNA. Translation: DAA08556.1.
PIRiS48354.
RefSeqiNP_012275.3. NM_001179532.3.

Genome annotation databases

EnsemblFungiiYIR010W; YIR010W; YIR010W.
GeneIDi854827.
KEGGisce:YIR010W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79743 Genomic DNA. No translation available.
Z37996 Genomic DNA. Translation: CAA86080.1.
BK006942 Genomic DNA. Translation: DAA08556.1.
PIRiS48354.
RefSeqiNP_012275.3. NM_001179532.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5T6JX-ray1.75C560-572[»]
ProteinModelPortaliP40568.
SMRiP40568.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35002. 639 interactors.
DIPiDIP-4794N.
IntActiP40568. 18 interactors.
MINTiMINT-546805.

PTM databases

iPTMnetiP40568.

Proteomic databases

MaxQBiP40568.
PRIDEiP40568.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIR010W; YIR010W; YIR010W.
GeneIDi854827.
KEGGisce:YIR010W.

Organism-specific databases

EuPathDBiFungiDB:YIR010W.
SGDiS000001449. DSN1.

Phylogenomic databases

HOGENOMiHOG000112286.
InParanoidiP40568.
KOiK11560.
OMAiIISPHKD.
OrthoDBiEOG092C5AKJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-31431-MONOMER.

Miscellaneous databases

PROiPR:P40568.

Family and domain databases

InterProiView protein in InterPro
IPR013218. Dsn1/Mis13.
IPR018247. EF_Hand_1_Ca_BS.
PANTHERiPTHR14778. PTHR14778. 1 hit.
PfamiView protein in Pfam
PF08202. MIS13. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDSN1_YEAST
AccessioniPrimary (citable) accession number: P40568
Secondary accession number(s): D6VVU0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 7, 2017
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.