P40567 (MSL1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
U2 small nuclear ribonucleoprotein B''
Short name=U2 snRNP B''

Gene names

Name:	MSL1
Ordered Locus Names:	YIR009W
ORF Names:	YIB9W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	111 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in pre-mRNA splicing. This protein is associated with snRNP U2. It binds stem loop IV of U2 snRNA. Ref.5
Subunit structure	Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with RDS3. Interacts with LEA1. Ref.6 Ref.7
Subcellular location	Nucleus.
Miscellaneous	Present with 504 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA splicing, via spliceosome Inferred from mutant phenotype Ref.6. Source: SGD
Cellular_component	U2 snRNP Traceable author statement PubMed 11804584. Source: SGD U2-type prespliceosome Inferred from direct assay PubMed 16618970. Source: SGD
Molecular_function	U2 snRNA binding Inferred from physical interaction Ref.5. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 111	111	U2 small nuclear ribonucleoprotein B''		PRO_0000081895
Regions
	Domain	28 – 106	79	RRM

Sequences

	Sequence		Length	Mass (Da)
	P40567 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 8FB0974FC30994AE	FASTA	111	12,830
	10 20 30 40 50 60 MVEPARKKQR IDRDTHHTVA EPVTEAKNTL YVSQLNEKIN MQRLRVNLFL LFATFGEVLK 70 80 90 100 110 VSMNFKKQRG QAFITMRTID QASLAQISLN GERFFGKPLK VEFSKSETKT L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and analysis of the centromeric region of yeast chromosome IX." Voss H., Tamames J., Teodoru C., Valencia A., Sensen C., Wiemann S., Schwager C., Zimmermann J., Sander C., Ansorge W. Yeast 11:61-78(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The reference genome sequence of Saccharomyces cerevisiae: Then and now." Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M. G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Identification and characterization of a yeast gene encoding the U2 small nuclear ribonucleoprotein particle B' protein." Tang J., Abovich N., Rosbash M. Mol. Cell. Biol. 16:2787-2795(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"The yeast U2A'/U2B complex is required for pre-spliceosome formation." Caspary F., Seraphin B. EMBO J. 17:6348-6358(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH LEA1.
[7]	"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs." Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L. Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X79743 Genomic DNA. No translation available. Z37996 Genomic DNA. Translation: CAA86079.1. AY558237 Genomic DNA. Translation: AAS56563.1. BK006942 Genomic DNA. Translation: DAA08555.1.
PIR	S48353.
RefSeq	NP_012274.1. NM_001179531.1.
3D structure databases
ProteinModelPortal	P40567.
SMR	P40567. Positions 29-108.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	35001. 71 interactions.
DIP	DIP-816N.
IntAct	P40567. 10 interactions.
MINT	MINT-572508.
STRING	4932.YIR009W.
Proteomic databases
MaxQB	P40567.
PaxDb	P40567.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIR009W; YIR009W; YIR009W.
GeneID	854826.
KEGG	sce:YIR009W.
Organism-specific databases
CYGD	YIR009w.
SGD	S000001448. MSL1.
Phylogenomic databases
eggNOG	NOG317159.
GeneTree	ENSGT00390000007046.
HOGENOM	HOG000000973.
OMA	RTIDQAS.
OrthoDB	EOG7DC2H6.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31430-MONOMER.
Gene expression databases
Genevestigator	P40567.
Family and domain databases
Gene3D	3.30.70.330. 1 hit.
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. IPR024888. U1_snRNP_A/U2_snRNP_B''. [Graphical view]
PANTHER	PTHR10501. PTHR10501. 1 hit.
SMART	SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977683.

Entry information

Entry name

MSL1_YEAST

Accession

Primary (citable) accession number: P40567
Secondary accession number(s): D6VVT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 14, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program