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Protein

U2 small nuclear ribonucleoprotein B''

Gene

MSL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing. This protein is associated with snRNP U2. It binds stem loop IV of U2 snRNA.1 Publication

Miscellaneous

Present with 504 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • U2 snRNA binding Source: SGD

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-31430-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
U2 small nuclear ribonucleoprotein B''
Short name:
U2 snRNP B''
Gene namesi
Name:MSL1
Ordered Locus Names:YIR009W
ORF Names:YIB9W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIR009W.
SGDiS000001448. MSL1.

Subcellular locationi

GO - Cellular componenti

  • U2 snRNP Source: SGD
  • U2-type prespliceosome Source: SGD

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000818951 – 111U2 small nuclear ribonucleoprotein B''Add BLAST111

Proteomic databases

MaxQBiP40567.
PRIDEiP40567.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with RDS3. Interacts with LEA1.2 Publications

Protein-protein interaction databases

BioGridi35001. 74 interactors.
DIPiDIP-816N.
IntActiP40567. 10 interactors.
MINTiMINT-572508.
STRINGi4932.YIR009W.

Structurei

Secondary structure

1111
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi30 – 34Combined sources5
Helixi41 – 53Combined sources13
Turni66 – 70Combined sources5
Beta strandi72 – 74Combined sources3
Helixi79 – 88Combined sources10
Beta strandi100 – 103Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GMKelectron microscopy3.40a1-111[»]
5LJ3electron microscopy3.80Y1-111[»]
5LJ5electron microscopy3.80Y1-111[»]
5MQ0electron microscopy4.17Y1-111[»]
5WSGelectron microscopy4.00X1-111[»]
ProteinModelPortaliP40567.
SMRiP40567.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 106RRMPROSITE-ProRule annotationAdd BLAST79

Phylogenomic databases

GeneTreeiENSGT00390000007046.
HOGENOMiHOG000000973.
OMAiFFDKPLV.
OrthoDBiEOG092C5A64.

Family and domain databases

InterProiView protein in InterPro
IPR000504. RRM_dom.
SMARTiView protein in SMART
SM00360. RRM. 1 hit.
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiView protein in PROSITE
PS50102. RRM. 1 hit.

Sequencei

Sequence statusi: Complete.

P40567-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEPARKKQR IDRDTHHTVA EPVTEAKNTL YVSQLNEKIN MQRLRVNLFL
60 70 80 90 100
LFATFGEVLK VSMNFKKQRG QAFITMRTID QASLAQISLN GERFFGKPLK
110
VEFSKSETKT L
Length:111
Mass (Da):12,830
Last modified:February 1, 1995 - v1
Checksum:i8FB0974FC30994AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79743 Genomic DNA. No translation available.
Z37996 Genomic DNA. Translation: CAA86079.1.
AY558237 Genomic DNA. Translation: AAS56563.1.
BK006942 Genomic DNA. Translation: DAA08555.1.
PIRiS48353.
RefSeqiNP_012274.1. NM_001179531.1.

Genome annotation databases

EnsemblFungiiYIR009W; YIR009W; YIR009W.
GeneIDi854826.
KEGGisce:YIR009W.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMSL1_YEAST
AccessioniPrimary (citable) accession number: P40567
Secondary accession number(s): D6VVT9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 5, 2017
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names