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P40567 (MSL1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
U2 small nuclear ribonucleoprotein B''

Short name=U2 snRNP B''
Gene names
Name:MSL1
Ordered Locus Names:YIR009W
ORF Names:YIB9W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length111 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in pre-mRNA splicing. This protein is associated with snRNP U2. It binds stem loop IV of U2 snRNA. Ref.5

Subunit structure

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with RDS3. Interacts with LEA1. Ref.6 Ref.7

Subcellular location

Nucleus.

Miscellaneous

Present with 504 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
   Biological processmRNA processing
mRNA splicing
   Cellular componentNucleus
   LigandRNA-binding
   Molecular functionRibonucleoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmRNA splicing, via spliceosome

Inferred from mutant phenotype Ref.6. Source: SGD

   Cellular_componentU2 snRNP

Traceable author statement PubMed 11804584. Source: SGD

U2-type prespliceosome

Inferred from direct assay PubMed 16618970. Source: SGD

   Molecular_functionU2 snRNA binding

Inferred from physical interaction Ref.5. Source: SGD

nucleotide binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 111111U2 small nuclear ribonucleoprotein B''
PRO_0000081895

Regions

Domain28 – 10679RRM

Sequences

Sequence LengthMass (Da)Tools
P40567 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 8FB0974FC30994AE

FASTA11112,830
        10         20         30         40         50         60 
MVEPARKKQR IDRDTHHTVA EPVTEAKNTL YVSQLNEKIN MQRLRVNLFL LFATFGEVLK 

        70         80         90        100        110 
VSMNFKKQRG QAFITMRTID QASLAQISLN GERFFGKPLK VEFSKSETKT L 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence and analysis of the centromeric region of yeast chromosome IX."
Voss H., Tamames J., Teodoru C., Valencia A., Sensen C., Wiemann S., Schwager C., Zimmermann J., Sander C., Ansorge W.
Yeast 11:61-78(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Identification and characterization of a yeast gene encoding the U2 small nuclear ribonucleoprotein particle B' protein."
Tang J., Abovich N., Rosbash M.
Mol. Cell. Biol. 16:2787-2795(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"The yeast U2A'/U2B complex is required for pre-spliceosome formation."
Caspary F., Seraphin B.
EMBO J. 17:6348-6358(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LEA1.
[7]"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X79743 Genomic DNA. No translation available.
Z37996 Genomic DNA. Translation: CAA86079.1.
AY558237 Genomic DNA. Translation: AAS56563.1.
BK006942 Genomic DNA. Translation: DAA08555.1.
PIRS48353.
RefSeqNP_012274.1. NM_001179531.1.

3D structure databases

ProteinModelPortalP40567.
SMRP40567. Positions 29-108.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35001. 71 interactions.
DIPDIP-816N.
IntActP40567. 10 interactions.
MINTMINT-572508.
STRING4932.YIR009W.

Proteomic databases

PaxDbP40567.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIR009W; YIR009W; YIR009W.
GeneID854826.
KEGGsce:YIR009W.

Organism-specific databases

CYGDYIR009w.
SGDS000001448. MSL1.

Phylogenomic databases

eggNOGNOG317159.
GeneTreeENSGT00390000007046.
HOGENOMHOG000000973.
OMARTIDQAS.
OrthoDBEOG7DC2H6.

Enzyme and pathway databases

BioCycYEAST:G3O-31430-MONOMER.

Gene expression databases

GenevestigatorP40567.

Family and domain databases

Gene3D3.30.70.330. 1 hit.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR024888. U1_snRNP_A/U2_snRNP_B''.
[Graphical view]
PANTHERPTHR10501. PTHR10501. 1 hit.
SMARTSM00360. RRM. 1 hit.
[Graphical view]
PROSITEPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977683.

Entry information

Entry nameMSL1_YEAST
AccessionPrimary (citable) accession number: P40567
Secondary accession number(s): D6VVT9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families