Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

U2 snRNP component IST3

Gene

IST3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for pre-mRNA splicing and spliceosome assembly. As part of the pre-mRNA retention and splicing (RES) complex, required for nuclear pre-mRNA retention and efficient splicing. Required for MER1-activated splicing.3 Publications

GO - Molecular functioni

GO - Biological processi

  • generation of catalytic spliceosome for first transesterification step Source: SGD
  • mRNA export from nucleus Source: SGD
  • mRNA splicing, via spliceosome Source: SGD
  • spliceosomal complex assembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31426-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
U2 snRNP component IST3
Alternative name(s):
Increased sodium tolerance protein 3
U2 snRNP protein SNU17
Gene namesi
Name:IST3
Synonyms:SNU17
Ordered Locus Names:YIR005W
ORF Names:YIB5W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIR005W.
SGDiS000001444. IST3.

Subcellular locationi

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: GO_Central
  • cytoplasm Source: UniProtKB-SubCell
  • precatalytic spliceosome Source: GO_Central
  • RES complex Source: SGD
  • U2 snRNP Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

Pathology & Biotechi

Disruption phenotypei

Cells have a slow growth phenotype that is exacerbated at 37 degrees Celsius. Deletion mutants have a significant pre-mRNA leakage at 25 degrees Celsius.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000816211 – 148U2 snRNP component IST3Add BLAST148

Proteomic databases

MaxQBiP40565.
PRIDEiP40565.

Interactioni

Subunit structurei

Component of the 45S U1.U2.U4/U6.U5 penta-snRNP particle, a subcomplex of the spliceosome. Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Belongs to the pre-mRNA retention and splicing (RES) complex composed of at least BUD13, IST3 and PML1. Subunit of the U2 snRNP. Interacts with RDS3.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BUD13P469476EBI-25387,EBI-24073

Protein-protein interaction databases

BioGridi34996. 199 interactors.
DIPiDIP-2076N.
IntActiP40565. 14 interactors.
MINTiMINT-389778.

Structurei

Secondary structure

1148
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi27 – 29Combined sources3
Beta strandi32 – 37Combined sources6
Helixi44 – 51Combined sources8
Helixi52 – 54Combined sources3
Beta strandi57 – 64Combined sources8
Beta strandi66 – 68Combined sources3
Beta strandi71 – 81Combined sources11
Helixi82 – 91Combined sources10
Beta strandi103 – 105Combined sources3
Helixi113 – 115Combined sources3
Helixi116 – 130Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MKCNMR-A25-138[»]
2MY2NMR-A25-138[»]
2MY3NMR-A25-138[»]
4UQTNMR-A25-113[»]
5GM6electron microscopy3.50V1-148[»]
5LQWelectron microscopy5.80J1-148[»]
ProteinModelPortaliP40565.
SMRiP40565.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 109RRMPROSITE-ProRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the IST3 family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00820000127083.
HOGENOMiHOG000174091.
InParanoidiP40565.
KOiK13107.
OMAiFAYLKYE.
OrthoDBiEOG092C3SCB.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40565-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKIQQINDK ELQSGILSPH QSWHNEYKDN AYIYIGNLNR ELTEGDILTV
60 70 80 90 100
FSEYGVPVDV ILSRDENTGE SQGFAYLKYE DQRSTILAVD NLNGFKIGGR
110 120 130 140
ALKIDHTFYR PKRSLQKYYE AVKEELDRDI VSKNNAEKLI LAKKDQPN
Length:148
Mass (Da):17,098
Last modified:February 1, 1995 - v1
Checksum:i9D51C3043AB823D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79743 Genomic DNA. No translation available.
Z38062 Genomic DNA. Translation: CAA86207.1.
AY558081 Genomic DNA. Translation: AAS56407.1.
BK006942 Genomic DNA. Translation: DAA08551.1.
PIRiS48439.
RefSeqiNP_012270.1. NM_001179527.1.

Genome annotation databases

EnsemblFungiiYIR005W; YIR005W; YIR005W.
GeneIDi854821.
KEGGisce:YIR005W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79743 Genomic DNA. No translation available.
Z38062 Genomic DNA. Translation: CAA86207.1.
AY558081 Genomic DNA. Translation: AAS56407.1.
BK006942 Genomic DNA. Translation: DAA08551.1.
PIRiS48439.
RefSeqiNP_012270.1. NM_001179527.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MKCNMR-A25-138[»]
2MY2NMR-A25-138[»]
2MY3NMR-A25-138[»]
4UQTNMR-A25-113[»]
5GM6electron microscopy3.50V1-148[»]
5LQWelectron microscopy5.80J1-148[»]
ProteinModelPortaliP40565.
SMRiP40565.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34996. 199 interactors.
DIPiDIP-2076N.
IntActiP40565. 14 interactors.
MINTiMINT-389778.

Proteomic databases

MaxQBiP40565.
PRIDEiP40565.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIR005W; YIR005W; YIR005W.
GeneIDi854821.
KEGGisce:YIR005W.

Organism-specific databases

EuPathDBiFungiDB:YIR005W.
SGDiS000001444. IST3.

Phylogenomic databases

GeneTreeiENSGT00820000127083.
HOGENOMiHOG000174091.
InParanoidiP40565.
KOiK13107.
OMAiFAYLKYE.
OrthoDBiEOG092C3SCB.

Enzyme and pathway databases

BioCyciYEAST:G3O-31426-MONOMER.

Miscellaneous databases

PROiP40565.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIST3_YEAST
AccessioniPrimary (citable) accession number: P40565
Secondary accession number(s): D6VVT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 922 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.