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P40565

- IST3_YEAST

UniProt

P40565 - IST3_YEAST

Protein

U2 snRNP component IST3

Gene

IST3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Required for pre-mRNA splicing and spliceosome assembly. As part of the pre-mRNA retention and splicing (RES) complex, required for nuclear pre-mRNA retention and efficient splicing. Required for MER1-activated splicing.3 Publications

    GO - Molecular functioni

    1. nucleotide binding Source: InterPro
    2. protein binding Source: IntAct
    3. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. generation of catalytic spliceosome for first transesterification step Source: SGD
    2. mRNA export from nucleus Source: SGD
    3. mRNA splicing, via spliceosome Source: SGD
    4. spliceosomal complex assembly Source: SGD

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31426-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    U2 snRNP component IST3
    Alternative name(s):
    Increased sodium tolerance protein 3
    U2 snRNP protein SNU17
    Gene namesi
    Name:IST3
    Synonyms:SNU17
    Ordered Locus Names:YIR005W
    ORF Names:YIB5W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIR005w.
    SGDiS000001444. IST3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. RES complex Source: SGD
    3. spliceosomal complex Source: UniProtKB-KW
    4. U2 snRNP Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Spliceosome

    Pathology & Biotechi

    Disruption phenotypei

    Cells have a slow growth phenotype that is exacerbated at 37 degrees Celsius. Deletion mutants have a significant pre-mRNA leakage at 25 degrees Celsius.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 148148U2 snRNP component IST3PRO_0000081621Add
    BLAST

    Proteomic databases

    MaxQBiP40565.
    PaxDbiP40565.
    PeptideAtlasiP40565.

    Expressioni

    Gene expression databases

    GenevestigatoriP40565.

    Interactioni

    Subunit structurei

    Component of the 45S U1.U2.U4/U6.U5 penta-snRNP particle, a subcomplex of the spliceosome. Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Belongs to the pre-mRNA retention and splicing (RES) complex composed of at least BUD13, IST3 and PML1. Subunit of the U2 snRNP. Interacts with RDS3.5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BUD13P469475EBI-25387,EBI-24073

    Protein-protein interaction databases

    BioGridi34996. 197 interactions.
    DIPiDIP-2076N.
    IntActiP40565. 14 interactions.
    MINTiMINT-389778.
    STRINGi4932.YIR005W.

    Structurei

    3D structure databases

    ProteinModelPortaliP40565.
    SMRiP40565. Positions 4-108.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – 10979RRMPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the IST3 family.Curated
    Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0724.
    GeneTreeiENSGT00730000110692.
    HOGENOMiHOG000174091.
    KOiK13107.
    OMAiRDKETHE.
    OrthoDBiEOG70W3S0.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view]
    PfamiPF00076. RRM_1. 1 hit.
    [Graphical view]
    SMARTiSM00360. RRM. 1 hit.
    [Graphical view]
    PROSITEiPS50102. RRM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40565-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNKIQQINDK ELQSGILSPH QSWHNEYKDN AYIYIGNLNR ELTEGDILTV    50
    FSEYGVPVDV ILSRDENTGE SQGFAYLKYE DQRSTILAVD NLNGFKIGGR 100
    ALKIDHTFYR PKRSLQKYYE AVKEELDRDI VSKNNAEKLI LAKKDQPN 148
    Length:148
    Mass (Da):17,098
    Last modified:February 1, 1995 - v1
    Checksum:i9D51C3043AB823D2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79743 Genomic DNA. No translation available.
    Z38062 Genomic DNA. Translation: CAA86207.1.
    AY558081 Genomic DNA. Translation: AAS56407.1.
    BK006942 Genomic DNA. Translation: DAA08551.1.
    PIRiS48439.
    RefSeqiNP_012270.1. NM_001179527.1.

    Genome annotation databases

    EnsemblFungiiYIR005W; YIR005W; YIR005W.
    GeneIDi854821.
    KEGGisce:YIR005W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79743 Genomic DNA. No translation available.
    Z38062 Genomic DNA. Translation: CAA86207.1 .
    AY558081 Genomic DNA. Translation: AAS56407.1 .
    BK006942 Genomic DNA. Translation: DAA08551.1 .
    PIRi S48439.
    RefSeqi NP_012270.1. NM_001179527.1.

    3D structure databases

    ProteinModelPortali P40565.
    SMRi P40565. Positions 4-108.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34996. 197 interactions.
    DIPi DIP-2076N.
    IntActi P40565. 14 interactions.
    MINTi MINT-389778.
    STRINGi 4932.YIR005W.

    Proteomic databases

    MaxQBi P40565.
    PaxDbi P40565.
    PeptideAtlasi P40565.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIR005W ; YIR005W ; YIR005W .
    GeneIDi 854821.
    KEGGi sce:YIR005W.

    Organism-specific databases

    CYGDi YIR005w.
    SGDi S000001444. IST3.

    Phylogenomic databases

    eggNOGi COG0724.
    GeneTreei ENSGT00730000110692.
    HOGENOMi HOG000174091.
    KOi K13107.
    OMAi RDKETHE.
    OrthoDBi EOG70W3S0.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31426-MONOMER.

    Miscellaneous databases

    NextBioi 977669.

    Gene expression databases

    Genevestigatori P40565.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view ]
    Pfami PF00076. RRM_1. 1 hit.
    [Graphical view ]
    SMARTi SM00360. RRM. 1 hit.
    [Graphical view ]
    PROSITEi PS50102. RRM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and analysis of the centromeric region of yeast chromosome IX."
      Voss H., Tamames J., Teodoru C., Valencia A., Sensen C., Wiemann S., Schwager C., Zimmermann J., Sander C., Ansorge W.
      Yeast 11:61-78(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "A novel yeast U2 snRNP protein, Snu17p, is required for the first catalytic step of splicing and for progression of spliceosome assembly."
      Gottschalk A., Bartels C., Neubauer G., Luehrmann R., Fabrizio P.
      Mol. Cell. Biol. 21:3037-3046(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
    6. "Composition and functional characterization of the yeast spliceosomal penta-snRNP."
      Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J.
      Mol. Cell 9:31-44(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX BY MASS SPECTROMETRY.
    7. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
      Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
      Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    8. "Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus."
      Wang Q., Rymond B.C.
      Mol. Cell. Biol. 23:7339-7349(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RDS3.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. "Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing."
      Dziembowski A., Ventura A.-P., Rutz B., Caspary F., Faux C., Halgand F., Laprevote O., Seraphin B.
      EMBO J. 23:4847-4856(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE RES COMPLEX BY MASS SPECTROMETRY, DISRUPTION PHENOTYPE.
    12. "Mer1p is a modular splicing factor whose function depends on the conserved U2 snRNP protein Snu17p."
      Spingola M., Armisen J., Ares M. Jr.
      Nucleic Acids Res. 32:1242-1250(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiIST3_YEAST
    AccessioniPrimary (citable) accession number: P40565
    Secondary accession number(s): D6VVT5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 922 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3