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P40565

- IST3_YEAST

UniProt

P40565 - IST3_YEAST

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Protein

U2 snRNP component IST3

Gene
IST3, SNU17, YIR005W, YIB5W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for pre-mRNA splicing and spliceosome assembly. As part of the pre-mRNA retention and splicing (RES) complex, required for nuclear pre-mRNA retention and efficient splicing. Required for MER1-activated splicing.3 Publications

GO - Molecular functioni

  1. nucleotide binding Source: InterPro
  2. protein binding Source: IntAct
  3. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. generation of catalytic spliceosome for first transesterification step Source: SGD
  2. mRNA export from nucleus Source: SGD
  3. mRNA splicing, via spliceosome Source: SGD
  4. spliceosomal complex assembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31426-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
U2 snRNP component IST3
Alternative name(s):
Increased sodium tolerance protein 3
U2 snRNP protein SNU17
Gene namesi
Name:IST3
Synonyms:SNU17
Ordered Locus Names:YIR005W
ORF Names:YIB5W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIR005w.
SGDiS000001444. IST3.

Subcellular locationi

Cytoplasm. Nucleus 2 Publications

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. RES complex Source: SGD
  3. spliceosomal complex Source: UniProtKB-KW
  4. U2 snRNP Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

Pathology & Biotechi

Disruption phenotypei

Cells have a slow growth phenotype that is exacerbated at 37 degrees Celsius. Deletion mutants have a significant pre-mRNA leakage at 25 degrees Celsius.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 148148U2 snRNP component IST3PRO_0000081621Add
BLAST

Proteomic databases

MaxQBiP40565.
PaxDbiP40565.
PeptideAtlasiP40565.

Expressioni

Gene expression databases

GenevestigatoriP40565.

Interactioni

Subunit structurei

Component of the 45S U1.U2.U4/U6.U5 penta-snRNP particle, a subcomplex of the spliceosome. Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Belongs to the pre-mRNA retention and splicing (RES) complex composed of at least BUD13, IST3 and PML1. Subunit of the U2 snRNP. Interacts with RDS3.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BUD13P469475EBI-25387,EBI-24073

Protein-protein interaction databases

BioGridi34996. 197 interactions.
DIPiDIP-2076N.
IntActiP40565. 14 interactions.
MINTiMINT-389778.
STRINGi4932.YIR005W.

Structurei

3D structure databases

ProteinModelPortaliP40565.
SMRiP40565. Positions 4-108.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 10979RRMAdd
BLAST

Sequence similaritiesi

Belongs to the IST3 family.

Phylogenomic databases

eggNOGiCOG0724.
GeneTreeiENSGT00730000110692.
HOGENOMiHOG000174091.
KOiK13107.
OMAiRDKETHE.
OrthoDBiEOG70W3S0.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40565-1 [UniParc]FASTAAdd to Basket

« Hide

MNKIQQINDK ELQSGILSPH QSWHNEYKDN AYIYIGNLNR ELTEGDILTV    50
FSEYGVPVDV ILSRDENTGE SQGFAYLKYE DQRSTILAVD NLNGFKIGGR 100
ALKIDHTFYR PKRSLQKYYE AVKEELDRDI VSKNNAEKLI LAKKDQPN 148
Length:148
Mass (Da):17,098
Last modified:February 1, 1995 - v1
Checksum:i9D51C3043AB823D2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X79743 Genomic DNA. No translation available.
Z38062 Genomic DNA. Translation: CAA86207.1.
AY558081 Genomic DNA. Translation: AAS56407.1.
BK006942 Genomic DNA. Translation: DAA08551.1.
PIRiS48439.
RefSeqiNP_012270.1. NM_001179527.1.

Genome annotation databases

EnsemblFungiiYIR005W; YIR005W; YIR005W.
GeneIDi854821.
KEGGisce:YIR005W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X79743 Genomic DNA. No translation available.
Z38062 Genomic DNA. Translation: CAA86207.1 .
AY558081 Genomic DNA. Translation: AAS56407.1 .
BK006942 Genomic DNA. Translation: DAA08551.1 .
PIRi S48439.
RefSeqi NP_012270.1. NM_001179527.1.

3D structure databases

ProteinModelPortali P40565.
SMRi P40565. Positions 4-108.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34996. 197 interactions.
DIPi DIP-2076N.
IntActi P40565. 14 interactions.
MINTi MINT-389778.
STRINGi 4932.YIR005W.

Proteomic databases

MaxQBi P40565.
PaxDbi P40565.
PeptideAtlasi P40565.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIR005W ; YIR005W ; YIR005W .
GeneIDi 854821.
KEGGi sce:YIR005W.

Organism-specific databases

CYGDi YIR005w.
SGDi S000001444. IST3.

Phylogenomic databases

eggNOGi COG0724.
GeneTreei ENSGT00730000110692.
HOGENOMi HOG000174091.
KOi K13107.
OMAi RDKETHE.
OrthoDBi EOG70W3S0.

Enzyme and pathway databases

BioCyci YEAST:G3O-31426-MONOMER.

Miscellaneous databases

NextBioi 977669.

Gene expression databases

Genevestigatori P40565.

Family and domain databases

Gene3Di 3.30.70.330. 1 hit.
InterProi IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view ]
Pfami PF00076. RRM_1. 1 hit.
[Graphical view ]
SMARTi SM00360. RRM. 1 hit.
[Graphical view ]
PROSITEi PS50102. RRM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and analysis of the centromeric region of yeast chromosome IX."
    Voss H., Tamames J., Teodoru C., Valencia A., Sensen C., Wiemann S., Schwager C., Zimmermann J., Sander C., Ansorge W.
    Yeast 11:61-78(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "A novel yeast U2 snRNP protein, Snu17p, is required for the first catalytic step of splicing and for progression of spliceosome assembly."
    Gottschalk A., Bartels C., Neubauer G., Luehrmann R., Fabrizio P.
    Mol. Cell. Biol. 21:3037-3046(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
  6. "Composition and functional characterization of the yeast spliceosomal penta-snRNP."
    Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J.
    Mol. Cell 9:31-44(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX BY MASS SPECTROMETRY.
  7. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
    Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
    Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  8. "Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus."
    Wang Q., Rymond B.C.
    Mol. Cell. Biol. 23:7339-7349(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RDS3.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing."
    Dziembowski A., Ventura A.-P., Rutz B., Caspary F., Faux C., Halgand F., Laprevote O., Seraphin B.
    EMBO J. 23:4847-4856(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE RES COMPLEX BY MASS SPECTROMETRY, DISRUPTION PHENOTYPE.
  12. "Mer1p is a modular splicing factor whose function depends on the conserved U2 snRNP protein Snu17p."
    Spingola M., Armisen J., Ares M. Jr.
    Nucleic Acids Res. 32:1242-1250(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiIST3_YEAST
AccessioniPrimary (citable) accession number: P40565
Secondary accession number(s): D6VVT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 922 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

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