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P40565 (IST3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
U2 snRNP component IST3
Alternative name(s):
Increased sodium tolerance protein 3
U2 snRNP protein SNU17
Gene names
Name:IST3
Synonyms:SNU17
Ordered Locus Names:YIR005W
ORF Names:YIB5W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length148 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for pre-mRNA splicing and spliceosome assembly. As part of the pre-mRNA retention and splicing (RES) complex, required for nuclear pre-mRNA retention and efficient splicing. Required for MER1-activated splicing. Ref.5 Ref.11 Ref.12

Subunit structure

Component of the 45S U1.U2.U4/U6.U5 penta-snRNP particle, a subcomplex of the spliceosome. Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Belongs to the pre-mRNA retention and splicing (RES) complex composed of at least BUD13, IST3 and PML1. Subunit of the U2 snRNP. Interacts with RDS3. Ref.5 Ref.6 Ref.7 Ref.8 Ref.11

Subcellular location

Cytoplasm. Nucleus Ref.5 Ref.9.

Disruption phenotype

Cells have a slow growth phenotype that is exacerbated at 37 degrees Celsius. Deletion mutants have a significant pre-mRNA leakage at 25 degrees Celsius. Ref.11

Miscellaneous

Present with 922 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the IST3 family.

Contains 1 RRM (RNA recognition motif) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BUD13P469475EBI-25387,EBI-24073

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 148148U2 snRNP component IST3
PRO_0000081621

Regions

Domain31 – 10979RRM

Sequences

Sequence LengthMass (Da)Tools
P40565 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 9D51C3043AB823D2

FASTA14817,098
        10         20         30         40         50         60 
MNKIQQINDK ELQSGILSPH QSWHNEYKDN AYIYIGNLNR ELTEGDILTV FSEYGVPVDV 

        70         80         90        100        110        120 
ILSRDENTGE SQGFAYLKYE DQRSTILAVD NLNGFKIGGR ALKIDHTFYR PKRSLQKYYE 

       130        140 
AVKEELDRDI VSKNNAEKLI LAKKDQPN 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence and analysis of the centromeric region of yeast chromosome IX."
Voss H., Tamames J., Teodoru C., Valencia A., Sensen C., Wiemann S., Schwager C., Zimmermann J., Sander C., Ansorge W.
Yeast 11:61-78(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"A novel yeast U2 snRNP protein, Snu17p, is required for the first catalytic step of splicing and for progression of spliceosome assembly."
Gottschalk A., Bartels C., Neubauer G., Luehrmann R., Fabrizio P.
Mol. Cell. Biol. 21:3037-3046(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
[6]"Composition and functional characterization of the yeast spliceosomal penta-snRNP."
Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J.
Mol. Cell 9:31-44(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX BY MASS SPECTROMETRY.
[7]"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[8]"Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus."
Wang Q., Rymond B.C.
Mol. Cell. Biol. 23:7339-7349(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RDS3.
[9]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing."
Dziembowski A., Ventura A.-P., Rutz B., Caspary F., Faux C., Halgand F., Laprevote O., Seraphin B.
EMBO J. 23:4847-4856(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE RES COMPLEX BY MASS SPECTROMETRY, DISRUPTION PHENOTYPE.
[12]"Mer1p is a modular splicing factor whose function depends on the conserved U2 snRNP protein Snu17p."
Spingola M., Armisen J., Ares M. Jr.
Nucleic Acids Res. 32:1242-1250(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X79743 Genomic DNA. No translation available.
Z38062 Genomic DNA. Translation: CAA86207.1.
AY558081 Genomic DNA. Translation: AAS56407.1.
BK006942 Genomic DNA. Translation: DAA08551.1.
PIRS48439.
RefSeqNP_012270.1. NM_001179527.1.

3D structure databases

ProteinModelPortalP40565.
SMRP40565. Positions 33-106.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34996. 197 interactions.
DIPDIP-2076N.
IntActP40565. 14 interactions.
MINTMINT-389778.
STRING4932.YIR005W.

Proteomic databases

PaxDbP40565.
PeptideAtlasP40565.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIR005W; YIR005W; YIR005W.
GeneID854821.
KEGGsce:YIR005W.

Organism-specific databases

CYGDYIR005w.
SGDS000001444. IST3.

Phylogenomic databases

eggNOGCOG0724.
GeneTreeENSGT00730000110692.
HOGENOMHOG000174091.
KOK13107.
OMARDKETHE.
OrthoDBEOG70W3S0.

Enzyme and pathway databases

BioCycYEAST:G3O-31426-MONOMER.

Gene expression databases

GenevestigatorP40565.

Family and domain databases

Gene3D3.30.70.330. 1 hit.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTSM00360. RRM. 1 hit.
[Graphical view]
PROSITEPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977669.

Entry information

Entry nameIST3_YEAST
AccessionPrimary (citable) accession number: P40565
Secondary accession number(s): D6VVT5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families