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Protein

ATP-dependent DNA helicase MPH1

Gene

MPH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent DNA helicase involved in DNA damage repair by homologous recombination and in genome maintenance (PubMed:10880470, PubMed:15126389, PubMed:15634678, PubMed:16121259). Capable of unwinding D-loops. Plays a role in limiting crossover recombinants during mitotic DNA double-strand break (DSB) repair (PubMed:19136626). Component of a FANCM-MHF complex which promotes gene conversion at blocked replication forks, probably by reversal of the stalled fork (PubMed:20347428).1 Publication5 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi107 – 1148ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • 3'-5' DNA helicase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • flap-structured DNA binding Source: SGD

GO - Biological processi

  • DNA replication, Okazaki fragment processing Source: SGD
  • interstrand cross-link repair Source: SGD
  • negative regulation of strand invasion Source: SGD
  • recombinational repair Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31423-MONOMER.
BRENDAi3.6.4.12. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase MPH1Curated (EC:3.6.4.121 Publication)
Alternative name(s):
FANCM-like protein 1By similarity
Mutator phenotype protein 11 Publication
Gene namesi
Name:MPH11 Publication
Ordered Locus Names:YIR002CImported
ORF Names:YIB2C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIR002C.
SGDiS000001441. MPH1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 993993ATP-dependent DNA helicase MPH1PRO_0000055193Add
BLAST

Proteomic databases

MaxQBiP40562.

PTM databases

iPTMnetiP40562.

Interactioni

Subunit structurei

Interacts with the MHF histone-fold complex composed of MHF1 and MHF2 to form the FANCM-MHF complex.By similarity

Protein-protein interaction databases

BioGridi34993. 93 interactions.
DIPiDIP-5016N.
IntActiP40562. 3 interactions.
MINTiMINT-493277.

Structurei

3D structure databases

ProteinModelPortaliP40562.
SMRiP40562. Positions 88-661.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini94 – 261168Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini507 – 655149Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi209 – 2124DEAH boxPROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00730000111048.
HOGENOMiHOG000066122.
InParanoidiP40562.
KOiK14635.
OMAiTEFTTKY.
OrthoDBiEOG092C15BQ.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40562-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASADDYFSD FEDDELDKLY EKAINKSVKE TITRRAVPVQ KDLHDNVLPG
60 70 80 90 100
QKTVYEEIQR DVSFGPTHHE LDYDALSFYV YPTNYEVRDY QYTIVHKSLF
110 120 130 140 150
QNTLCAIPTG MGKTFIASTV MLNYFRWTKK AKIIFTAPTR PLVAQQIKAC
160 170 180 190 200
LGITGIPSDQ TAILLDKSRK NREEIWANKR VFFATPQVVE NDLKRGVLDP
210 220 230 240 250
KDIVCLVIDE AHRATGSSAY TNVVKFIDRF NSSYRLLALT ATPASDLEGV
260 270 280 290 300
QEVVNNLDIS KIEIRTEESM DIVKYMKKRK KEKIEVPLLL EIEDIIEQLG
310 320 330 340 350
MAVKPVLQQA IELGIYEECD PSQINAFKAM QQSQKIIANP TIPEGIKWRN
360 370 380 390 400
FFILQLLNNV GQMLKRLKIY GIRTFFNYFQ NKCTEFTTKY NLKKSTNKIA
410 420 430 440 450
AEFYYHPILK NIKNQCENYL SDPKFVGHGK LQCVRDELMD FFQKRGSDSR
460 470 480 490 500
VIIFTELRES ALEIVKFIDS VADDQIRPHI FIGQARAKEG FDEVKYTRKH
510 520 530 540 550
APKGRKKVER LHRQEQEKFL EAERTKRAAN DKLERSARRT GSSEEAQISG
560 570 580 590 600
MNQKMQKEVI HNFKKGEYNV LVCTSIGEEG LDIGEVDLII CYDTTSSPIK
610 620 630 640 650
NIQRMGRTGR KRDGKIVLLF SSNESYKFER AMEDYSTLQA LISKQCIDYK
660 670 680 690 700
KSDRIIPEDI IPECHETLIT INDENEIINE MEDVDEVIRY ATQCMMGKKV
710 720 730 740 750
KPKKAITKKK RVQENKKPKK FFMPDNVETS IVSASTLINK FLVNESGGKQ
760 770 780 790 800
LVTSNENPSK KRKIFKALDN LENDSTEEAS SSLETEDEEV SDDNNVFIAE
810 820 830 840 850
GQNGCQKDLE TAIIRTGESL TTLKPLHNFE RPNMALFVND CGLPTKIEKN
860 870 880 890 900
VKDIRGNQHN LEKEKSCTVD KNNMVLSLDD WNFFRNRYIP EGVSFDVEPN
910 920 930 940 950
FVQYTKGVKV PHCHKVSKII TLFNDESNDN KKRTIDMNYT KCLARGMLRD
960 970 980 990
EKKFVKVNDK SQVDNNSVNH DSSQSFTLSN AELDDILGSD SDF
Length:993
Mass (Da):114,058
Last modified:February 1, 1995 - v1
Checksum:i474DDC99C543171F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79743 Genomic DNA. No translation available.
Z38062 Genomic DNA. Translation: CAA86204.1.
BK006942 Genomic DNA. Translation: DAA08548.1.
PIRiS48436.
RefSeqiNP_012267.1. NM_001179524.1.

Genome annotation databases

EnsemblFungiiYIR002C; YIR002C; YIR002C.
GeneIDi854818.
KEGGisce:YIR002C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79743 Genomic DNA. No translation available.
Z38062 Genomic DNA. Translation: CAA86204.1.
BK006942 Genomic DNA. Translation: DAA08548.1.
PIRiS48436.
RefSeqiNP_012267.1. NM_001179524.1.

3D structure databases

ProteinModelPortaliP40562.
SMRiP40562. Positions 88-661.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34993. 93 interactions.
DIPiDIP-5016N.
IntActiP40562. 3 interactions.
MINTiMINT-493277.

PTM databases

iPTMnetiP40562.

Proteomic databases

MaxQBiP40562.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIR002C; YIR002C; YIR002C.
GeneIDi854818.
KEGGisce:YIR002C.

Organism-specific databases

EuPathDBiFungiDB:YIR002C.
SGDiS000001441. MPH1.

Phylogenomic databases

GeneTreeiENSGT00730000111048.
HOGENOMiHOG000066122.
InParanoidiP40562.
KOiK14635.
OMAiTEFTTKY.
OrthoDBiEOG092C15BQ.

Enzyme and pathway databases

BioCyciYEAST:G3O-31423-MONOMER.
BRENDAi3.6.4.12. 984.

Miscellaneous databases

PROiP40562.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPH1_YEAST
AccessioniPrimary (citable) accession number: P40562
Secondary accession number(s): D6VVT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.