P40559 (INP51_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 4,5-bisphosphate 5-phosphatase INP51
EC=3.1.3.36

Alternative name(s):
Synaptojanin-like protein 1

Gene names

Name:	INP51
Synonyms:	SJL1
Ordered Locus Names:	YIL002C
ORF Names:	YIA2C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	946 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Controls the cellular levels and subcellular distribution of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Does not utilize phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2), nor phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 4-phosphate (PtdIns4P). Plays an essential role in a TGN (trans Golgi network)-to-early endosome pathway. Involved in endocytosis and acts as a negative regulator of the Slm pathway which modulates polarized actin assembly and growth. Ref.4 Ref.5 Ref.6 Ref.7 Ref.10 Ref.11 Ref.12 Ref.13
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.
Enzyme regulation	IRS4 and TAX4 are both positive regulator of INP51 activity and phosphatidylinositol 4,5-bisphosphate turnover. Ref.10
Subunit structure	Interacts with IRS4 and TAX4. Ref.10
Subcellular location	Cytoplasm › cytoskeleton › actin patch Ref.8.
Miscellaneous	Present with 98 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the synaptojanin family. In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family. Contains 1 SAC domain.

Ontologies

Keywords
Biological process	Endocytosis Lipid metabolism Protein transport Transport
Cellular component	Cytoplasm Cytoskeleton
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endocytosis Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylinositol dephosphorylation Inferred from direct assay PubMed 10224048. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	actin cortical patch Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from direct assay Ref.13. Source: SGD membrane Inferred from direct assay Ref.5. Source: SGD
Molecular_function	phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity Inferred from direct assay PubMed 10224048. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
IRS4	P36115	3	EBI-24915,EBI-27090
TAX4	P47030	3	EBI-24915,EBI-25970

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 946	946	Phosphatidylinositol 4,5-bisphosphate 5-phosphatase INP51		PRO_0000209741
Regions
	Domain	151 – 480	330	SAC

Sequences

	Sequence		Length	Mass (Da)
	P40559 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: A833C39B0A62543F	FASTA	946	108,430
	10 20 30 40 50 60 MRLFIGRRSR SIVISSNNYC LSFQRLRSIP GASSQQRQLS KTPSVTIKSY PDTDLSSDSN 70 80 90 100 110 120 YLEVKSCIFN GLLGLVCLNG DIYVAVISGV QNVGFPRWKL IDHQVRPSES IYKVLDVDFY 130 140 150 160 170 180 SLENDVFDYL LCERSEQNYD KLIHEHPCGP LKKLFSDGTF YYSRDFDISN IVKNHGLSHN 190 200 210 220 230 240 LEYTVDNQDL SFIWNANLAS EVINWRSKIS NEEKQLFANA GFLTFVIRGY CKTALIEDGP 250 260 270 280 290 300 NTASITIISR ISTESKQDTL ELEGISEDGR VSLFVETEIV VTTEKFIFSY TQVNGSIPLF 310 320 330 340 350 360 WESVESQLLY GKKIKVTKDS IEAQGAFDRH FDNLTSKYGV VSIVNIIKPK SESQEKLALT 370 380 390 400 410 420 YKDCAESKGI KITNIEYSSS VLTKSPHKLL YLLKQDIYEF GAFAYDISRG IYFAKQTGVL 430 440 450 460 470 480 RISAFDSIEK PNTVERLVSK EVLELTTNEI DVFELTSPFL DAHDKLWSEN YYWLDRTYTK 490 500 510 520 530 540 HTKNSGKYTK VYSKLFGSRV RLYDPLHIYI SQYLKQLRSK YTFEKDISIF AGTFNISGKI 550 560 570 580 590 600 PKDDIKDWIF PKSMSKEDEM ADLYVIGLEE VVELTPGHML ATDPYVRQFW EKKILTLLNG 610 620 630 640 650 660 PGRKKKYIRL WSTQLGGILL LLFMNETEYS KVKHIEGDVK KTGFGGMASN KGAVAVSFKY 670 680 690 700 710 720 SATRFCVLVS HLAAGLENVE QRHNDYKTIA KSIRFSKGLR IKDHDAIIWM GDFNYRILMS 730 740 750 760 770 780 NEDVRRKIVS KEYASLFEKD QLNQQMIAGE SFPYFHEMAI DFPPTYKFDP GTKNYDTSEK 790 800 810 820 830 840 MRIPAWTDRI LSRGEVLEQL EYKCCEDILF SDHRPVYAIF RARVTVVDEQ KKTTLGTQIY 850 860 870 880 890 900 EKIMERLEGL DDDEKIAVLS DDAFVIESFE GSDSIAGPTH SPTPIPEPKR GRKLPPPSSD 910 920 930 940 LKKWWIGSGK QVKVVLDVDP AVYMINPKRD PNPFVENEDE PLFIER

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and analysis of the centromeric region of yeast chromosome IX." Voss H., Tamames J., Teodoru C., Valencia A., Sensen C., Wiemann S., Schwager C., Zimmermann J., Sander C., Ansorge W. Yeast 11:61-78(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Novel genes involved in endosomal traffic in yeast revealed by suppression of a targeting-defective plasma membrane ATPase mutant." Luo W.-J., Chang A. J. Cell Biol. 138:731-746(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Identification and characterization of an essential family of inositol polyphosphate 5-phosphatases (INP51, INP52 and INP53 gene products) in the yeast Saccharomyces cerevisiae." Stolz L.E., Huynh C.V., Thorner J., York J.D. Genetics 148:1715-1729(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Synaptojanin family members are implicated in endocytic membrane traffic in yeast." Singer-Krueger B., Nemoto Y., Daniell L., Ferro-Novick S., De Camilli P. J. Cell Sci. 111:3347-3356(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Mammalian inositol polyphosphate 5-phosphatase II can compensate for the absence of all three yeast Sac1-like-domain-containing 5-phosphatases." O'Malley C.J., McColl B.K., Kong A.M., Ellis S.L., Wijayaratnam A.P.W., Sambrook J., Mitchell C.A. Biochem. J. 355:805-817(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Negative regulation of phosphatidylinositol 4,5-bisphosphate levels by the INP51-associated proteins TAX4 and IRS4." Morales-Johansson H., Jenoe P., Cooke F.T., Hall M.N. J. Biol. Chem. 279:39604-39610(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ENZYME REGULATION, INTERACTION WITH IRS4 AND TAX4.
[11]	"Interaction of Pik1p and Sjl proteins in membrane trafficking." Nguyen P.H., Hasek J., Kohlwein S.D., Romero C., Choi J.H., Vancura A. FEMS Yeast Res. 5:363-371(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"The pleckstrin homology domain proteins Slm1 and Slm2 are required for actin cytoskeleton organization in yeast and bind phosphatidylinositol-4,5-bisphosphate and TORC2." Fadri M., Daquinag A., Wang S., Xue T., Kunz J. Mol. Biol. Cell 16:1883-1900(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[13]	"PtdIns(4,5)P2 turnover is required for multiple stages during clathrin-and actin-dependent endocytic internalization." Sun Y., Carroll S., Kaksonen M., Toshima J.Y., Drubin D.G. J. Cell Biol. 177:355-367(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X79743 Genomic DNA. No translation available. Z38062 Genomic DNA. Translation: CAA86201.1. BK006942 Genomic DNA. Translation: DAA08545.1.
PIR	S48433.
RefSeq	NP_012264.3. NM_001179352.3.
3D structure databases
ProteinModelPortal	P40559.
SMR	P40559. Positions 503-842.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2677N.
IntAct	P40559. 8 interactions.
MINT	MINT-1651757.
STRING	4932.YIL002C.
Proteomic databases
PaxDb	P40559.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL002C; YIL002C; YIL002C.
GeneID	854815.
KEGG	sce:YIL002C.
Organism-specific databases
CYGD	YIL002c.
SGD	S000001264. INP51.
Phylogenomic databases
eggNOG	COG5411.
GeneTree	ENSGT00700000104268.
HOGENOM	HOG000179717.
OMA	ENVEQRH.
OrthoDB	EOG4Q5CXK.
Enzyme and pathway databases
BioCyc	YEAST:MONOMER3O-111. YEAST:YIL002C-MONOMER.
Gene expression databases
Genevestigator	P40559.
GermOnline	YIL002C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.60.10.10. 1 hit.
InterPro	IPR005135. Endo/exonuclease/phosphatase. IPR000300. IPPc. IPR002013. Syja_N. [Graphical view]
Pfam	PF03372. Exo_endo_phos. 1 hit. PF02383. Syja_N. 1 hit. [Graphical view]
SMART	SM00128. IPPc. 1 hit. [Graphical view]
SUPFAM	SSF56219. Exo_endo_phos. 1 hit.
PROSITE	PS50275. SAC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977651.

Entry information

Entry name

INP51_YEAST

Accession

Primary (citable) accession number: P40559
Secondary accession number(s): D6VVS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program