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Protein

Phosphatidylinositol 4,5-bisphosphate 5-phosphatase INP51

Gene

INP51

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Controls the cellular levels and subcellular distribution of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Does not utilize phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2), nor phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 4-phosphate (PtdIns4P). Plays an essential role in a TGN (trans Golgi network)-to-early endosome pathway. Involved in endocytosis and acts as a negative regulator of the Slm pathway which modulates polarized actin assembly and growth.8 Publications

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.

Enzyme regulationi

IRS4 and TAX4 are both positive regulator of INP51 activity and phosphatidylinositol 4,5-bisphosphate turnover.1 Publication

GO - Molecular functioni

  • phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity Source: SGD

GO - Biological processi

  • endocytosis Source: UniProtKB-KW
  • phosphatidylinositol dephosphorylation Source: SGD
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Endocytosis, Lipid metabolism, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:MONOMER3O-111.
YEAST:YIL002C-MONOMER.
ReactomeiR-SCE-1660499. Synthesis of PIPs at the plasma membrane.
R-SCE-1660514. Synthesis of PIPs at the Golgi membrane.
R-SCE-1855183. Synthesis of IP2, IP, and Ins in the cytosol.
R-SCE-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-SCE-194840. Rho GTPase cycle.
R-SCE-202424. Downstream TCR signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 4,5-bisphosphate 5-phosphatase INP51 (EC:3.1.3.36)
Alternative name(s):
Synaptojanin-like protein 1
Gene namesi
Name:INP51
Synonyms:SJL1
Ordered Locus Names:YIL002C
ORF Names:YIA2C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL002C.
SGDiS000001264. INP51.

Subcellular locationi

GO - Cellular componenti

  • actin cortical patch Source: UniProtKB-SubCell
  • cytoplasm Source: SGD
  • membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 946946Phosphatidylinositol 4,5-bisphosphate 5-phosphatase INP51PRO_0000209741Add
BLAST

Proteomic databases

MaxQBiP40559.

PTM databases

iPTMnetiP40559.

Interactioni

Subunit structurei

Interacts with IRS4 and TAX4.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
IRS4P361153EBI-24915,EBI-27090
TAX4P470303EBI-24915,EBI-25970

Protein-protein interaction databases

BioGridi34990. 71 interactions.
DIPiDIP-2677N.
IntActiP40559. 9 interactions.
MINTiMINT-1651757.

Structurei

3D structure databases

ProteinModelPortaliP40559.
SMRiP40559. Positions 82-470, 503-842.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini151 – 480330SACPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptojanin family.Curated
In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.Curated
Contains 1 SAC domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00760000119075.
HOGENOMiHOG000179717.
InParanoidiP40559.
OMAiFIWNANL.
OrthoDBiEOG7DRJBN.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
IPR000300. IPPc.
IPR002013. SAC_dom.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
PF02383. Syja_N. 1 hit.
[Graphical view]
SMARTiSM00128. IPPc. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
PROSITEiPS50275. SAC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40559-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLFIGRRSR SIVISSNNYC LSFQRLRSIP GASSQQRQLS KTPSVTIKSY
60 70 80 90 100
PDTDLSSDSN YLEVKSCIFN GLLGLVCLNG DIYVAVISGV QNVGFPRWKL
110 120 130 140 150
IDHQVRPSES IYKVLDVDFY SLENDVFDYL LCERSEQNYD KLIHEHPCGP
160 170 180 190 200
LKKLFSDGTF YYSRDFDISN IVKNHGLSHN LEYTVDNQDL SFIWNANLAS
210 220 230 240 250
EVINWRSKIS NEEKQLFANA GFLTFVIRGY CKTALIEDGP NTASITIISR
260 270 280 290 300
ISTESKQDTL ELEGISEDGR VSLFVETEIV VTTEKFIFSY TQVNGSIPLF
310 320 330 340 350
WESVESQLLY GKKIKVTKDS IEAQGAFDRH FDNLTSKYGV VSIVNIIKPK
360 370 380 390 400
SESQEKLALT YKDCAESKGI KITNIEYSSS VLTKSPHKLL YLLKQDIYEF
410 420 430 440 450
GAFAYDISRG IYFAKQTGVL RISAFDSIEK PNTVERLVSK EVLELTTNEI
460 470 480 490 500
DVFELTSPFL DAHDKLWSEN YYWLDRTYTK HTKNSGKYTK VYSKLFGSRV
510 520 530 540 550
RLYDPLHIYI SQYLKQLRSK YTFEKDISIF AGTFNISGKI PKDDIKDWIF
560 570 580 590 600
PKSMSKEDEM ADLYVIGLEE VVELTPGHML ATDPYVRQFW EKKILTLLNG
610 620 630 640 650
PGRKKKYIRL WSTQLGGILL LLFMNETEYS KVKHIEGDVK KTGFGGMASN
660 670 680 690 700
KGAVAVSFKY SATRFCVLVS HLAAGLENVE QRHNDYKTIA KSIRFSKGLR
710 720 730 740 750
IKDHDAIIWM GDFNYRILMS NEDVRRKIVS KEYASLFEKD QLNQQMIAGE
760 770 780 790 800
SFPYFHEMAI DFPPTYKFDP GTKNYDTSEK MRIPAWTDRI LSRGEVLEQL
810 820 830 840 850
EYKCCEDILF SDHRPVYAIF RARVTVVDEQ KKTTLGTQIY EKIMERLEGL
860 870 880 890 900
DDDEKIAVLS DDAFVIESFE GSDSIAGPTH SPTPIPEPKR GRKLPPPSSD
910 920 930 940
LKKWWIGSGK QVKVVLDVDP AVYMINPKRD PNPFVENEDE PLFIER
Length:946
Mass (Da):108,430
Last modified:February 1, 1995 - v1
Checksum:iA833C39B0A62543F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79743 Genomic DNA. No translation available.
Z38062 Genomic DNA. Translation: CAA86201.1.
BK006942 Genomic DNA. Translation: DAA08545.1.
PIRiS48433.
RefSeqiNP_012264.3. NM_001179352.3.

Genome annotation databases

EnsemblFungiiYIL002C; YIL002C; YIL002C.
GeneIDi854815.
KEGGisce:YIL002C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79743 Genomic DNA. No translation available.
Z38062 Genomic DNA. Translation: CAA86201.1.
BK006942 Genomic DNA. Translation: DAA08545.1.
PIRiS48433.
RefSeqiNP_012264.3. NM_001179352.3.

3D structure databases

ProteinModelPortaliP40559.
SMRiP40559. Positions 82-470, 503-842.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34990. 71 interactions.
DIPiDIP-2677N.
IntActiP40559. 9 interactions.
MINTiMINT-1651757.

PTM databases

iPTMnetiP40559.

Proteomic databases

MaxQBiP40559.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL002C; YIL002C; YIL002C.
GeneIDi854815.
KEGGisce:YIL002C.

Organism-specific databases

EuPathDBiFungiDB:YIL002C.
SGDiS000001264. INP51.

Phylogenomic databases

GeneTreeiENSGT00760000119075.
HOGENOMiHOG000179717.
InParanoidiP40559.
OMAiFIWNANL.
OrthoDBiEOG7DRJBN.

Enzyme and pathway databases

BioCyciYEAST:MONOMER3O-111.
YEAST:YIL002C-MONOMER.
ReactomeiR-SCE-1660499. Synthesis of PIPs at the plasma membrane.
R-SCE-1660514. Synthesis of PIPs at the Golgi membrane.
R-SCE-1855183. Synthesis of IP2, IP, and Ins in the cytosol.
R-SCE-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-SCE-194840. Rho GTPase cycle.
R-SCE-202424. Downstream TCR signaling.

Miscellaneous databases

NextBioi977651.
PROiP40559.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
IPR000300. IPPc.
IPR002013. SAC_dom.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
PF02383. Syja_N. 1 hit.
[Graphical view]
SMARTiSM00128. IPPc. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
PROSITEiPS50275. SAC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and analysis of the centromeric region of yeast chromosome IX."
    Voss H., Tamames J., Teodoru C., Valencia A., Sensen C., Wiemann S., Schwager C., Zimmermann J., Sander C., Ansorge W.
    Yeast 11:61-78(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Novel genes involved in endosomal traffic in yeast revealed by suppression of a targeting-defective plasma membrane ATPase mutant."
    Luo W.-J., Chang A.
    J. Cell Biol. 138:731-746(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Identification and characterization of an essential family of inositol polyphosphate 5-phosphatases (INP51, INP52 and INP53 gene products) in the yeast Saccharomyces cerevisiae."
    Stolz L.E., Huynh C.V., Thorner J., York J.D.
    Genetics 148:1715-1729(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Synaptojanin family members are implicated in endocytic membrane traffic in yeast."
    Singer-Krueger B., Nemoto Y., Daniell L., Ferro-Novick S., De Camilli P.
    J. Cell Sci. 111:3347-3356(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Mammalian inositol polyphosphate 5-phosphatase II can compensate for the absence of all three yeast Sac1-like-domain-containing 5-phosphatases."
    O'Malley C.J., McColl B.K., Kong A.M., Ellis S.L., Wijayaratnam A.P.W., Sambrook J., Mitchell C.A.
    Biochem. J. 355:805-817(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Negative regulation of phosphatidylinositol 4,5-bisphosphate levels by the INP51-associated proteins TAX4 and IRS4."
    Morales-Johansson H., Jenoe P., Cooke F.T., Hall M.N.
    J. Biol. Chem. 279:39604-39610(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, INTERACTION WITH IRS4 AND TAX4.
  11. "Interaction of Pik1p and Sjl proteins in membrane trafficking."
    Nguyen P.H., Hasek J., Kohlwein S.D., Romero C., Choi J.H., Vancura A.
    FEMS Yeast Res. 5:363-371(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "The pleckstrin homology domain proteins Slm1 and Slm2 are required for actin cytoskeleton organization in yeast and bind phosphatidylinositol-4,5-bisphosphate and TORC2."
    Fadri M., Daquinag A., Wang S., Xue T., Kunz J.
    Mol. Biol. Cell 16:1883-1900(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "PtdIns(4,5)P2 turnover is required for multiple stages during clathrin-and actin-dependent endocytic internalization."
    Sun Y., Carroll S., Kaksonen M., Toshima J.Y., Drubin D.G.
    J. Cell Biol. 177:355-367(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiINP51_YEAST
AccessioniPrimary (citable) accession number: P40559
Secondary accession number(s): D6VVS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 98 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.