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Protein

Cytosolic Fe-S cluster assembly factor CFD1

Gene

CFD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NBP35-CFD1 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Nucleotide binding/hydrolysis seems to be critcal for loading of Fe-S clusters onto CFD1 and NBP35. Required for biogenesis and export of both ribosomal subunits, which may reflect a role in assembly of the Fe/S clusters in RLI1, a protein which performs rRNA processing and ribosome export.UniRule annotation4 Publications

Cofactori

[4Fe-4S] clusterNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NBP35 and two labile, bridging clusters between subunits of the NBP35-CFD1 heterotetramer.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi201Iron-sulfur (4Fe-4S); shared with dimeric partner1
Metal bindingi204Iron-sulfur (4Fe-4S); shared with dimeric partner1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi25 – 32ATPUniRule annotation8

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: SGD
  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • iron-sulfur cluster assembly Source: SGD
  • small molecule metabolic process Source: Reactome
  • tRNA wobble uridine modification Source: SGD

Keywordsi

Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31282-MONOMER.
ReactomeiR-SCE-2564818. Cytosolic iron-sulfur cluster assembly (yeast).
R-SCE-2564830. Cytosolic iron-sulfur cluster assembly.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor CFD1UniRule annotation
Alternative name(s):
Cytosolic Fe-S cluster-deficient protein 1UniRule annotation
Ribosomal export protein 19
Gene namesi
Name:CFD1UniRule annotation
Synonyms:DRE3, RIX19
Ordered Locus Names:YIL003W
ORF Names:YIA3W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL003W.
SGDiS000001265. CFD1.

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytosol Source: Reactome
  • Nbp35-Cfd1 ATPase complex Source: SGD

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi31K → A: Loss of function. 1
Mutagenesisi86T → A: Loss of function. 1
Mutagenesisi182C → A: Does not impair function. 1 Publication1
Mutagenesisi201C → A: Loss of function and disrupts heterotetramer formation. 1 Publication1
Mutagenesisi204C → A: Loss of function and disrupts heterotetramer formation. 1 Publication1
Mutagenesisi207C → A: Does not impair function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001849521 – 293Cytosolic Fe-S cluster assembly factor CFD1Add BLAST293

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei291PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40558.
PRIDEiP40558.

PTM databases

iPTMnetiP40558.

Interactioni

Subunit structurei

Heterotetramer of 2 NBP35 and 2 CFD1 chains.UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NBP35P529209EBI-24924,EBI-11880

Protein-protein interaction databases

BioGridi34989. 248 interactors.
IntActiP40558. 3 interactors.
STRINGi4932.YIL003W.

Structurei

3D structure databases

ProteinModelPortaliP40558.
SMRiP40558.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP2/CFD1 subfamily.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000075057.
HOGENOMiHOG000079916.
InParanoidiP40558.
OMAiDVLWDET.
OrthoDBiEOG092C4BLN.

Family and domain databases

CDDicd02037. MRP-like. 1 hit.
HAMAPiMF_02040. Mrp_NBP35. 1 hit.
MF_03039. NUBP2. 1 hit.
InterProiView protein in InterPro
IPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028600. NUBP2/Cfd1_eukaryotes.
IPR027417. P-loop_NTPase.
IPR033756. YlxH/NBP35.
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiView protein in Pfam
PF10609. ParA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS01215. MRP. 1 hit.

Sequencei

Sequence statusi: Complete.

P40558-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEQEIGVPA ASLAGIKHII LILSGKGGVG KSSVTTQTAL TLCSMGFKVG
60 70 80 90 100
VLDIDLTGPS LPRMFGLENE SIYQGPEGWQ PVKVETNSTG SLSVISLGFL
110 120 130 140 150
LGDRGNSVIW RGPKKTSMIK QFISDVAWGE LDYLLIDTPP GTSDEHISIA
160 170 180 190 200
EELRYSKPDG GIVVTTPQSV ATADVKKEIN FCKKVDLKIL GIIENMSGFV
210 220 230 240 250
CPHCAECTNI FSSGGGKRLS EQFSVPYLGN VPIDPKFVEM IENQVSSKKT
260 270 280 290
LVEMYRESSL CPIFEEIMKK LRKQDTTTPV VDKHEQPQIE SPK
Length:293
Mass (Da):31,922
Last modified:February 1, 1995 - v1
Checksum:i5CDD563B51229D14
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79743 Genomic DNA. No translation available.
Z38113 Genomic DNA. Translation: CAA86248.1.
Z38062 Genomic DNA. Translation: CAA86200.1.
AY558297 Genomic DNA. Translation: AAS56623.1.
BK006942 Genomic DNA. Translation: DAA08543.1.
PIRiS58704.
RefSeqiNP_012263.1. NM_001179353.1.

Genome annotation databases

EnsemblFungiiYIL003W; YIL003W; YIL003W.
GeneIDi854814.
KEGGisce:YIL003W.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCFD1_YEAST
AccessioniPrimary (citable) accession number: P40558
Secondary accession number(s): D6VVS7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 5, 2017
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names