Reviewed, UniProtKB/Swiss-Prot P40557 (YIA5_YEAST)

Names and origin

Protein names

Recommended name:
    Putative protein disulfide-isomerase YIL005W
    EC=5.3.4.1

Gene names

Ordered Locus Names:	YIL005W
ORF Names:	YIA5W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	701 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Miscellaneous	Present with 6020 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 2 thioredoxin domains.

Ontologies

Keywords
Cellular component	Membrane
Domain	Redox-active center Repeat Signal Transmembrane
Molecular function	Isomerase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ER-associated protein catabolic process Inferred from mutant phenotype. Source: SGD cell redox homeostasis Inferred from electronic annotation. Source: InterPro protein retention in ER lumen Inferred from mutant phenotype. Source: SGD
Cellular component	endoplasmic reticulum membrane Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chaperone binding Inferred from direct assay. Source: SGD protein disulfide isomerase activity Inferred from mutant phenotype. Source: SGD unfolded protein binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 701	674	Putative protein disulfide-isomerase YIL005W		PRO_0000034221
Regions
	Transmembrane	646 – 666	21	Potential
	Domain	28 – 142	115	Thioredoxin
	Domain	408 – 446	39	Thioredoxin 2
Amino acid modifications
	Glycosylation	85	1	N-linked (GlcNAc...) Potential
	Glycosylation	264	1	N-linked (GlcNAc...) Potential
	Glycosylation	299	1	N-linked (GlcNAc...) Potential
	Glycosylation	370	1	N-linked (GlcNAc...) Potential
	Disulfide bond	60 ↔ 63		Redox-active By similarity
	Disulfide bond	200 ↔ 203		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P40557-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 31666693790E04EB	FASTA	701	81,219
	10 20 30 40 50 60 MKMNLKRLVV TFFSCITFLL KFTIAAAEPP EGFPEPLNPT NFKEELSKGL HIIDFYSPYC 70 80 90 100 110 120 PHCKHLAPVW METWEEFKEE SKTLNITFSQ VNCIESADLC GDENIEYFPE IRLYNPSGYI 130 140 150 160 170 180 KSFTETPRTK ESLIAFARRE SMDPNNLDTD LDSAKSESQY LEGFDFLELI AGKATRPHLV 190 200 210 220 230 240 SFWPTKDMKN SDDSLEFKNC DKCHEFQRTW KIISRQLAVD DINTGHVNCE SNPTICEELG 250 260 270 280 290 300 FGDLVKITNH RADREPKVAL VLPNKTSNNL FDYPNGYSAK SDGYVDFARR TFTNSKFPNI 310 320 330 340 350 360 TEGELEKKAN RDIDFLQERG RVTNNDIHLV FSYDPETVVI EDFDILEYLI EPLSKIPNIY 370 380 390 400 410 420 LHQIDKNLIN LSRNLFGRMY EKINYDASQT QKVFNKEYFT MNTVTQLPTF FMFKDGDPIS 430 440 450 460 470 480 YVFPGYSTTE MRNIDAIMDW VKKYSNPLVT EVDSSNLKKL ISFQTKSYSD LAIQLISSTD 490 500 510 520 530 540 HKHIKGSNKL IKNLLLASWE YEHIRMENNF EEINERRARK ADGIKKIKEK KAPANKIVDK 550 560 570 580 590 600 MREEIPHMDQ KKLLLGYLDI SKEKNFFRKY GITGEYKIGD VIIIDKSNNY YYNKDNFGNS 610 620 630 640 650 660 LTSNNPQLLR EAFVSLNIPS KALYSSKLKG RLINSPFHNV LSFLDIIHGN GMPGYLIVIV 670 680 690 700 LFIAILKGPS IYRRYKVRKH YRAKRNAVGI LGNMEKKKNQ D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed: 9169870] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Nucleotide sequence and analysis of the centromeric region of yeast chromosome IX." Voss H., Tamames J., Teodoru C., Valencia A., Sensen C., Wiemann S., Schwager C., Zimmermann J., Sander C., Ansorge W. Yeast 11:61-78(1995) [PubMed: 7762303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 549-701. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z38113 Genomic DNA. Translation: CAA86246.1. AY723829 Genomic DNA. Translation: AAU09746.1. X79743 Genomic DNA. No translation available.
PIR	S48452.
RefSeq	NP_012261.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3860N.
IntAct	P40557. 3 interactions.
STRING	P40557.
Proteomic databases
PeptideAtlas	P40557.
Genome annotation databases
Ensembl	YIL005W; YIL005W; YIL005W; Saccharomyces cerevisiae. [Genome view]
GeneID	854812.
GenomeReviews	Gene locus YIL005W in contig Z47047_GR.
KEGG	sce:YIL005W.
NMPDR	fig|4932.3.peg.1798.
Organism-specific databases
CYGD	YIL005w.
SGD	S000001267. EPS1.
Phylogenomic databases
HOGENOM	P40557.
OMA	PYCHHCK.
Enzyme and pathway databases
BRENDA	5.3.4.1. 250.
Gene expression databases
ArrayExpress	P40557.
Genevestigator	P40557.
GermOnline	YIL005W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR017936. Thioredoxin-like. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00085. Thioredoxin. 1 hit. [Graphical view]
PROSITE	PS00194. THIOREDOXIN_1. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	977642.

Entry information

Entry name

YIA5_YEAST

Accession

Primary (citable) accession number: P40557

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 3, 2009
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)