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Protein

Mitochondrial nicotinamide adenine dinucleotide transporter 1

Gene

YIA6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial inner membrane carrier protein involved in the import of NAD+ into mitochondria by unidirectional transport or by exchange with intramitochondrially generated dAMP and dGMP.3 Publications

Kineticsi

  1. KM=0.38 mM for NAD+1 Publication

Vmax=617 nmol/min/mg enzyme1 Publication

GO - Molecular functioni

  1. NAD transporter activity Source: SGD
  2. pyruvate secondary active transmembrane transporter activity Source: SGD
  3. transporter activity Source: SGD

GO - Biological processi

  1. mitochondrial pyruvate transport Source: SGD
  2. NAD transmembrane transport Source: SGD
  3. pyruvate transmembrane transport Source: GOC
  4. transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31285-MONOMER.

Protein family/group databases

TCDBi2.A.29.10.5. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial nicotinamide adenine dinucleotide transporter 1
Alternative name(s):
Mitochondrial NAD(+) transporter 1
Gene namesi
Name:YIA6
Synonyms:NDT1
Ordered Locus Names:YIL006W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IX

Organism-specific databases

CYGDiYIL006w.
EuPathDBiFungiDB:YIL006W.
SGDiS000001268. YIA6.

Subcellular locationi

  1. Mitochondrion inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8080Mitochondrial matrixSequence AnalysisAdd
BLAST
Transmembranei81 – 10121Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini102 – 14140Mitochondrial intermembraneSequence AnalysisAdd
BLAST
Transmembranei142 – 16221Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini163 – 17614Mitochondrial matrixSequence AnalysisAdd
BLAST
Transmembranei177 – 19923Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini200 – 23536Mitochondrial intermembraneSequence AnalysisAdd
BLAST
Transmembranei236 – 25621Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini257 – 28024Mitochondrial matrixSequence AnalysisAdd
BLAST
Transmembranei281 – 29717Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini298 – 33538Mitochondrial intermembraneSequence AnalysisAdd
BLAST
Transmembranei336 – 35823Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini359 – 37315Mitochondrial matrixSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: SGD
  3. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 373373Mitochondrial nicotinamide adenine dinucleotide transporter 1PRO_0000090698Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP40556.

Interactioni

Protein-protein interaction databases

BioGridi34986. 12 interactions.
DIPiDIP-4960N.
MINTiMINT-570805.
STRINGi4932.YIL006W.

Structurei

3D structure databases

ProteinModelPortaliP40556.
SMRiP40556. Positions 82-359.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati75 – 16692Solcar 1Add
BLAST
Repeati174 – 26390Solcar 2Add
BLAST
Repeati276 – 36489Solcar 3Add
BLAST

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG314559.
GeneTreeiENSGT00770000120566.
HOGENOMiHOG000188210.
InParanoidiP40556.
KOiK15115.
OMAiWAIYFTM.
OrthoDBiEOG73Z352.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40556-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQTDNPVPN CGLLPEQQYC SADHEEPLLL HEEQLIFPDH SSQLSSADII
60 70 80 90 100
EPIKMNSSTE SIIGTTLRKK WVPLSSTQIT ALSGAFAGFL SGVAVCPLDV
110 120 130 140 150
AKTRLQAQGL QTRFENPYYR GIMGTLSTIV RDEGPRGLYK GLVPIVLGYF
160 170 180 190 200
PTWMIYFSVY EFSKKFFHGI FPQFDFVAQS CAAITAGAAS TTLTNPIWVV
210 220 230 240 250
KTRLMLQSNL GEHPTHYKGT FDAFRKLFYQ EGFKALYAGL VPSLLGLFHV
260 270 280 290 300
AIHFPIYEDL KVRFHCYSRE NNTNSINLQR LIMASSVSKM IASAVTYPHE
310 320 330 340 350
ILRTRMQLKS DIPDSIQRRL FPLIKATYAQ EGLKGFYSGF TTNLVRTIPA
360 370
SAITLVSFEY FRNRLENIST MVI
Length:373
Mass (Da):41,954
Last modified:February 1, 1995 - v1
Checksum:i976C767C1D40E8DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38113 Genomic DNA. Translation: CAA86245.1.
BK006942 Genomic DNA. Translation: DAA08540.1.
PIRiS48451.
RefSeqiNP_012260.1. NM_001179356.1.

Genome annotation databases

EnsemblFungiiYIL006W; YIL006W; YIL006W.
GeneIDi854811.
KEGGisce:YIL006W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38113 Genomic DNA. Translation: CAA86245.1.
BK006942 Genomic DNA. Translation: DAA08540.1.
PIRiS48451.
RefSeqiNP_012260.1. NM_001179356.1.

3D structure databases

ProteinModelPortaliP40556.
SMRiP40556. Positions 82-359.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34986. 12 interactions.
DIPiDIP-4960N.
MINTiMINT-570805.
STRINGi4932.YIL006W.

Protein family/group databases

TCDBi2.A.29.10.5. the mitochondrial carrier (mc) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL006W; YIL006W; YIL006W.
GeneIDi854811.
KEGGisce:YIL006W.

Organism-specific databases

CYGDiYIL006w.
EuPathDBiFungiDB:YIL006W.
SGDiS000001268. YIA6.

Phylogenomic databases

eggNOGiNOG314559.
GeneTreeiENSGT00770000120566.
HOGENOMiHOG000188210.
InParanoidiP40556.
KOiK15115.
OMAiWAIYFTM.
OrthoDBiEOG73Z352.

Enzyme and pathway databases

BioCyciYEAST:G3O-31285-MONOMER.

Miscellaneous databases

NextBioi977639.
PROiP40556.

Gene expression databases

GenevestigatoriP40556.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Phylogenetic classification of the mitochondrial carrier family of Saccharomyces cerevisiae."
    el Moualij B., Duyckaerts C., Lamotte-Brasseur J., Sluse F.E.
    Yeast 13:573-581(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Identification of the mitochondrial pyruvate carrier in Saccharomyces cerevisiae."
    Hildyard J.C., Halestrap A.P.
    Biochem. J. 374:607-611(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Identification of the mitochondrial NAD+ transporter in Saccharomyces cerevisiae."
    Todisco S., Agrimi G., Castegna A., Palmieri F.
    J. Biol. Chem. 281:1524-1531(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
  6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiYIA6_YEAST
AccessioniPrimary (citable) accession number: P40556
Secondary accession number(s): D6VVS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 29, 2015
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

Was first identified as the mitochondrial pyruvate transporter (PubMed:12887330). However, later experiments showed that was a NAD+ transporter (PubMed:16291748).2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.