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P40556 (YIA6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial nicotinamide adenine dinucleotide transporter 1
Alternative name(s):
Mitochondrial NAD(+) transporter 1
Gene names
Name:YIA6
Synonyms:NDT1
Ordered Locus Names:YIL006W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mitochondrial inner membrane carrier protein involved in the import of NAD+ into mitochondria by unidirectional transport or by exchange with intramitochondrially generated dAMP and dGMP. Ref.3 Ref.4 Ref.5

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein Ref.5.

Sequence similarities

Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification]

Contains 3 Solcar repeats.

Caution

Was first identified as the mitochondrial pyruvate transporter (Ref.4). However, later experiments showed that was a NAD+ transporter (Ref.5).

Biophysicochemical properties

Kinetic parameters:

KM=0.38 mM for NAD+ Ref.5

Vmax=617 nmol/min/mg enzyme

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 373373Mitochondrial nicotinamide adenine dinucleotide transporter 1
PRO_0000090698

Regions

Topological domain1 – 8080Mitochondrial matrix Potential
Transmembrane81 – 10121Helical; Name=1; Potential
Topological domain102 – 14140Mitochondrial intermembrane Potential
Transmembrane142 – 16221Helical; Name=2; Potential
Topological domain163 – 17614Mitochondrial matrix Potential
Transmembrane177 – 19923Helical; Name=3; Potential
Topological domain200 – 23536Mitochondrial intermembrane Potential
Transmembrane236 – 25621Helical; Name=4; Potential
Topological domain257 – 28024Mitochondrial matrix Potential
Transmembrane281 – 29717Helical; Name=5; Potential
Topological domain298 – 33538Mitochondrial intermembrane Potential
Transmembrane336 – 35823Helical; Name=6; Potential
Topological domain359 – 37315Mitochondrial matrix Potential
Repeat75 – 16692Solcar 1
Repeat174 – 26390Solcar 2
Repeat276 – 36489Solcar 3

Sequences

Sequence LengthMass (Da)Tools
P40556 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 976C767C1D40E8DF

FASTA37341,954
        10         20         30         40         50         60 
MTQTDNPVPN CGLLPEQQYC SADHEEPLLL HEEQLIFPDH SSQLSSADII EPIKMNSSTE 

        70         80         90        100        110        120 
SIIGTTLRKK WVPLSSTQIT ALSGAFAGFL SGVAVCPLDV AKTRLQAQGL QTRFENPYYR 

       130        140        150        160        170        180 
GIMGTLSTIV RDEGPRGLYK GLVPIVLGYF PTWMIYFSVY EFSKKFFHGI FPQFDFVAQS 

       190        200        210        220        230        240 
CAAITAGAAS TTLTNPIWVV KTRLMLQSNL GEHPTHYKGT FDAFRKLFYQ EGFKALYAGL 

       250        260        270        280        290        300 
VPSLLGLFHV AIHFPIYEDL KVRFHCYSRE NNTNSINLQR LIMASSVSKM IASAVTYPHE 

       310        320        330        340        350        360 
ILRTRMQLKS DIPDSIQRRL FPLIKATYAQ EGLKGFYSGF TTNLVRTIPA SAITLVSFEY 

       370 
FRNRLENIST MVI

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Phylogenetic classification of the mitochondrial carrier family of Saccharomyces cerevisiae."
el Moualij B., Duyckaerts C., Lamotte-Brasseur J., Sluse F.E.
Yeast 13:573-581(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Identification of the mitochondrial pyruvate carrier in Saccharomyces cerevisiae."
Hildyard J.C., Halestrap A.P.
Biochem. J. 374:607-611(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Identification of the mitochondrial NAD+ transporter in Saccharomyces cerevisiae."
Todisco S., Agrimi G., Castegna A., Palmieri F.
J. Biol. Chem. 281:1524-1531(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
[6]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z38113 Genomic DNA. Translation: CAA86245.1.
BK006942 Genomic DNA. Translation: DAA08540.1.
PIRS48451.
RefSeqNP_012260.1. NM_001179356.1.

3D structure databases

ProteinModelPortalP40556.
SMRP40556. Positions 82-359.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-4960N.
MINTMINT-570805.
STRING4932.YIL006W.

Protein family/group databases

TCDB2.A.29.10.5. mitochondrial carrier (MC) family.
2.A.29.27.1. mitochondrial carrier (MC) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIL006W; YIL006W; YIL006W.
GeneID854811.
KEGGsce:YIL006W.

Organism-specific databases

CYGDYIL006w.
SGDS000001268. YIA6.

Phylogenomic databases

eggNOGNOG314559.
GeneTreeENSGT00600000084419.
HOGENOMHOG000188210.
KOK15115.
OMAMLTYEYL.
OrthoDBEOG4CG3JQ.

Gene expression databases

GenevestigatorP40556.
GermOnlineYIL006W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D1.50.40.10. 1 hit.
InterProIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSPR00926. MITOCARRIER.
SUPFAMSSF103506. Mitoch_carrier. 1 hit.
PROSITEPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio977639.

Entry information

Entry nameYIA6_YEAST
AccessionPrimary (citable) accession number: P40556
Secondary accession number(s): D6VVS4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents