P40555 (PSMD9_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 26S proteasome regulatory subunit p27

Alternative name(s):
Proteasome non-ATPase subunit 2

Gene names

Name:	NAS2
Ordered Locus Names:	YIL007C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the 19S regulatory complex (RC). During the base subcomplex assembly is part of a NAS2:RPT4:RPT5 module; NAS2 is released during the further base assembly process. Ref.6
Subunit structure	Part of a transient complex containing NAS2, RPT4 and RPT5 formed during the assembly of the 26S proteasome.
Miscellaneous	Present with 846 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the proteasome subunit p27 family. Contains 1 PDZ (DHR) domain.

Ontologies

Keywords
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteasome regulatory particle assembly Inferred from direct assay Ref.6. Source: SGD ubiquitin-dependent protein catabolic process Inferred from sequence or structural similarity PubMed 10419517. Source: SGD
Cellular_component	cytosol Inferred from direct assay PubMed 19446323. Source: SGD nucleus Inferred from direct assay PubMed 19446323. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PFS1	P38872	3	EBI-14024,EBI-24859
RPT5	P33297	6	EBI-14024,EBI-13920

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Probable 26S proteasome regulatory subunit p27		PRO_0000173856
Regions
	Domain	119 – 196	78	PDZ
	Compositional bias	2 – 5	4	Poly-Glu

Sequences

	Sequence		Length	Mass (Da)
	P40555 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 1BA966C5B7B2D02B	FASTA	220	24,846
	10 20 30 40 50 60 MEEEELSKLL ANVKIDPSLT SRISQIDSFK LSELMVLKTD IETQLEAYFS VLEQQGIGMD 70 80 90 100 110 120 SALVTPDGYP RSDVDVLQVT MIRKNVNMLK NDLNHLLQRS HVLLNQHFDN MNVKSNQDAR 130 140 150 160 170 180 RNNDDQAIQY TIPFAFISEV VPGSPSDKAD IKVDDKLISI GNVHAANHSK LQNIQMVVMK 190 200 210 220 NEDRPLPVLL LREGQILKTS LTPSRNWNGR GLLGCRIQEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"cDNA cloning and characterization of a human proteasomal modulator subunit, p27 (PSMD9)." Watanabe T.K., Saito A., Suzuki M., Fujiwara T., Takahashi E., Slaughter C.A., DeMartino G.N., Hendil K.B., Chung C.H., Tanahashi N., Tanaka K. Genomics 50:241-250(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Multiple assembly chaperones govern biogenesis of the proteasome regulatory particle base." Funakoshi M., Tomko R.J. Jr., Kobayashi H., Hochstrasser M. Cell 137:887-899(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS PROTEASOME CHAPERONE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z38113 Genomic DNA. Translation: CAA86244.1. AY558294 Genomic DNA. Translation: AAS56620.1. BK006942 Genomic DNA. Translation: DAA08539.1.
PIR	S48450.
RefSeq	NP_012259.3. NM_001179357.3.
3D structure databases
ProteinModelPortal	P40555.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1893N.
IntAct	P40555. 11 interactions.
MINT	MINT-394729.
STRING	4932.YIL007C.
Proteomic databases
PaxDb	P40555.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL007C; YIL007C; YIL007C.
GeneID	854810.
KEGG	sce:YIL001W. sce:YIL007C.
Organism-specific databases
CYGD	YIL007c.
SGD	S000001269. NAS2.
Phylogenomic databases
eggNOG	COG0265.
GeneTree	ENSGT00390000004147.
HOGENOM	HOG000216665.
KO	K06693. K06867.
OMA	LLGCNIV.
OrthoDB	EOG4ZCXF4.
Gene expression databases
Genevestigator	P40555.
GermOnline	YIL007C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001478. PDZ. [Graphical view]
Pfam	PF00595. PDZ. 1 hit. [Graphical view]
SMART	SM00228. PDZ. 1 hit. [Graphical view]
SUPFAM	SSF50156. PDZ. 1 hit.
PROSITE	PS50106. PDZ. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	977636.

Entry information

Entry name

PSMD9_YEAST

Accession

Primary (citable) accession number: P40555
Secondary accession number(s): D6VVS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program