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Protein

Probable 26S proteasome regulatory subunit p27

Gene

NAS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the 19S regulatory complex (RC). During the base subcomplex assembly is part of a NAS2:RPT4:RPT5 module; NAS2 is released during the further base assembly process.1 Publication

Miscellaneous

Present with 846 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • proteasome regulatory particle assembly Source: SGD
  • ubiquitin-dependent protein catabolic process Source: SGD

Keywordsi

Molecular functionChaperone

Enzyme and pathway databases

BioCyciYEAST:G3O-31286-MONOMER
ReactomeiR-SCE-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-SCE-174113 SCF-beta-TrCP mediated degradation of Emi1
R-SCE-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-SCE-382556 ABC-family proteins mediated transport
R-SCE-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-SCE-5668541 TNFR2 non-canonical NF-kB pathway
R-SCE-5687128 MAPK6/MAPK4 signaling
R-SCE-5689880 Ub-specific processing proteases
R-SCE-68949 Orc1 removal from chromatin
R-SCE-69017 CDK-mediated phosphorylation and removal of Cdc6
R-SCE-69229 Ubiquitin-dependent degradation of Cyclin D1
R-SCE-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-SCE-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 26S proteasome regulatory subunit p27
Alternative name(s):
Proteasome non-ATPase subunit 2
Gene namesi
Name:NAS2
Ordered Locus Names:YIL007C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL007C
SGDiS000001269 NAS2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001738561 – 220Probable 26S proteasome regulatory subunit p27Add BLAST220

Proteomic databases

MaxQBiP40555
PaxDbiP40555
PRIDEiP40555

Interactioni

Subunit structurei

Part of a transient complex containing NAS2, RPT4 and RPT5 formed during the assembly of the 26S proteasome.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34985125 interactors.
DIPiDIP-1893N
IntActiP40555 20 interactors.
MINTiP40555
STRINGi4932.YIL007C

Structurei

Secondary structure

1220
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 11Combined sources10
Helixi17 – 24Combined sources8
Turni25 – 28Combined sources4
Helixi31 – 54Combined sources24
Helixi73 – 110Combined sources38
Beta strandi135 – 140Combined sources6
Helixi145 – 148Combined sources4
Beta strandi156 – 160Combined sources5
Turni165 – 167Combined sources3
Turni169 – 171Combined sources3
Helixi172 – 180Combined sources9
Turni181 – 183Combined sources3
Beta strandi186 – 192Combined sources7
Beta strandi195 – 201Combined sources7
Beta strandi207 – 213Combined sources7
Beta strandi215 – 219Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WHJX-ray1.65A1-120[»]
3WHLX-ray4.00B/D/F/H1-120[»]
4O06X-ray1.15A126-220[»]
ProteinModelPortaliP40555
SMRiP40555
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini119 – 196PDZAdd BLAST78

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 5Poly-Glu4

Sequence similaritiesi

Belongs to the proteasome subunit p27 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000004147
HOGENOMiHOG000216665
InParanoidiP40555
KOiK06693
OMAiLGCNIVP
OrthoDBiEOG092C5E72

Family and domain databases

InterProiView protein in InterPro
IPR001478 PDZ
IPR036034 PDZ_sf
IPR035269 PSMD9
PANTHERiPTHR12651 PTHR12651, 1 hit
PfamiView protein in Pfam
PF00595 PDZ, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SUPFAMiSSF50156 SSF50156, 1 hit

Sequencei

Sequence statusi: Complete.

P40555-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEEELSKLL ANVKIDPSLT SRISQIDSFK LSELMVLKTD IETQLEAYFS
60 70 80 90 100
VLEQQGIGMD SALVTPDGYP RSDVDVLQVT MIRKNVNMLK NDLNHLLQRS
110 120 130 140 150
HVLLNQHFDN MNVKSNQDAR RNNDDQAIQY TIPFAFISEV VPGSPSDKAD
160 170 180 190 200
IKVDDKLISI GNVHAANHSK LQNIQMVVMK NEDRPLPVLL LREGQILKTS
210 220
LTPSRNWNGR GLLGCRIQEL
Length:220
Mass (Da):24,846
Last modified:February 1, 1995 - v1
Checksum:i1BA966C5B7B2D02B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38113 Genomic DNA Translation: CAA86244.1
AY558294 Genomic DNA Translation: AAS56620.1
BK006942 Genomic DNA Translation: DAA08539.1
PIRiS48450
RefSeqiNP_012259.3, NM_001179357.3

Genome annotation databases

EnsemblFungiiYIL007C; YIL007C; YIL007C
GeneIDi854810
KEGGisce:YIL007C

Similar proteinsi

Entry informationi

Entry nameiPSMD9_YEAST
AccessioniPrimary (citable) accession number: P40555
Secondary accession number(s): D6VVS3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome