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P40555 (PSMD9_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable 26S proteasome regulatory subunit p27
Alternative name(s):
Proteasome non-ATPase subunit 2
Gene names
Name:NAS2
Ordered Locus Names:YIL007C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length220 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the 19S regulatory complex (RC). During the base subcomplex assembly is part of a NAS2:RPT4:RPT5 module; NAS2 is released during the further base assembly process. Ref.6

Subunit structure

Part of a transient complex containing NAS2, RPT4 and RPT5 formed during the assembly of the 26S proteasome.

Miscellaneous

Present with 846 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the proteasome subunit p27 family.

Contains 1 PDZ (DHR) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PFS1P388723EBI-14024,EBI-24859
RPT5P332976EBI-14024,EBI-13920

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 220220Probable 26S proteasome regulatory subunit p27
PRO_0000173856

Regions

Domain119 – 19678PDZ
Compositional bias2 – 54Poly-Glu

Sequences

Sequence LengthMass (Da)Tools
P40555 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 1BA966C5B7B2D02B

FASTA22024,846
        10         20         30         40         50         60 
MEEEELSKLL ANVKIDPSLT SRISQIDSFK LSELMVLKTD IETQLEAYFS VLEQQGIGMD 

        70         80         90        100        110        120 
SALVTPDGYP RSDVDVLQVT MIRKNVNMLK NDLNHLLQRS HVLLNQHFDN MNVKSNQDAR 

       130        140        150        160        170        180 
RNNDDQAIQY TIPFAFISEV VPGSPSDKAD IKVDDKLISI GNVHAANHSK LQNIQMVVMK 

       190        200        210        220 
NEDRPLPVLL LREGQILKTS LTPSRNWNGR GLLGCRIQEL 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"cDNA cloning and characterization of a human proteasomal modulator subunit, p27 (PSMD9)."
Watanabe T.K., Saito A., Suzuki M., Fujiwara T., Takahashi E., Slaughter C.A., DeMartino G.N., Hendil K.B., Chung C.H., Tanahashi N., Tanaka K.
Genomics 50:241-250(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Multiple assembly chaperones govern biogenesis of the proteasome regulatory particle base."
Funakoshi M., Tomko R.J. Jr., Kobayashi H., Hochstrasser M.
Cell 137:887-899(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS PROTEASOME CHAPERONE.
[7]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z38113 Genomic DNA. Translation: CAA86244.1.
AY558294 Genomic DNA. Translation: AAS56620.1.
BK006942 Genomic DNA. Translation: DAA08539.1.
PIRS48450.
RefSeqNP_012259.3. NM_001179357.3.

3D structure databases

ProteinModelPortalP40555.
SMRP40555. Positions 133-203.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34985. 31 interactions.
DIPDIP-1893N.
IntActP40555. 10 interactions.
MINTMINT-394729.
STRING4932.YIL007C.

Proteomic databases

PaxDbP40555.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIL007C; YIL007C; YIL007C.
GeneID854810.
KEGGsce:YIL007C.

Organism-specific databases

CYGDYIL007c.
SGDS000001269. NAS2.

Phylogenomic databases

eggNOGCOG0265.
GeneTreeENSGT00390000004147.
HOGENOMHOG000216665.
KOK06693.
OMALLGCNIV.
OrthoDBEOG7V1G2H.

Enzyme and pathway databases

BioCycYEAST:G3O-31286-MONOMER.

Gene expression databases

GenevestigatorP40555.

Family and domain databases

Gene3D2.30.42.10. 1 hit.
InterProIPR001478. PDZ.
[Graphical view]
PfamPF00595. PDZ. 1 hit.
[Graphical view]
SMARTSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMSSF50156. SSF50156. 1 hit.
ProtoNetSearch...

Other

NextBio977636.
PROP40555.

Entry information

Entry namePSMD9_YEAST
AccessionPrimary (citable) accession number: P40555
Secondary accession number(s): D6VVS3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families