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Protein

Probable 26S proteasome regulatory subunit p27

Gene

NAS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the 19S regulatory complex (RC). During the base subcomplex assembly is part of a NAS2:RPT4:RPT5 module; NAS2 is released during the further base assembly process.1 Publication

GO - Biological processi

  • proteasome regulatory particle assembly Source: SGD
  • ubiquitin-dependent protein catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

BioCyciYEAST:G3O-31286-MONOMER.
ReactomeiR-SCE-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-SCE-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SCE-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-SCE-5668541. TNFR2 non-canonical NF-kB pathway.
R-SCE-5687128. MAPK6/MAPK4 signaling.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SCE-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SCE-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 26S proteasome regulatory subunit p27
Alternative name(s):
Proteasome non-ATPase subunit 2
Gene namesi
Name:NAS2
Ordered Locus Names:YIL007C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL007C.
SGDiS000001269. NAS2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytosol Source: SGD
  • nucleus Source: SGD
  • proteasome regulatory particle, base subcomplex Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220Probable 26S proteasome regulatory subunit p27PRO_0000173856Add
BLAST

Proteomic databases

MaxQBiP40555.

Interactioni

Subunit structurei

Part of a transient complex containing NAS2, RPT4 and RPT5 formed during the assembly of the 26S proteasome.

Binary interactionsi

WithEntry#Exp.IntActNotes
PFS1P388723EBI-14024,EBI-24859
RPT5P332976EBI-14024,EBI-13920

Protein-protein interaction databases

BioGridi34985. 31 interactions.
DIPiDIP-1893N.
IntActiP40555. 10 interactions.
MINTiMINT-394729.

Structurei

Secondary structure

1
220
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 1110Combined sources
Helixi17 – 248Combined sources
Turni25 – 284Combined sources
Helixi31 – 5424Combined sources
Helixi73 – 11038Combined sources
Beta strandi135 – 1406Combined sources
Helixi145 – 1484Combined sources
Beta strandi156 – 1605Combined sources
Turni165 – 1673Combined sources
Turni169 – 1713Combined sources
Helixi172 – 1809Combined sources
Turni181 – 1833Combined sources
Beta strandi186 – 1927Combined sources
Beta strandi195 – 2017Combined sources
Beta strandi207 – 2137Combined sources
Beta strandi215 – 2195Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3WHJX-ray1.65A1-120[»]
3WHLX-ray4.00B/D/F/H1-120[»]
4O06X-ray1.15A126-220[»]
ProteinModelPortaliP40555.
SMRiP40555. Positions 1-117, 129-220.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini119 – 19678PDZAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 54Poly-Glu

Sequence similaritiesi

Belongs to the proteasome subunit p27 family.Curated
Contains 1 PDZ (DHR) domain.Curated

Phylogenomic databases

GeneTreeiENSGT00390000004147.
HOGENOMiHOG000216665.
InParanoidiP40555.
KOiK06693.
OMAiLGCNIVP.
OrthoDBiEOG7V1G2H.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
[Graphical view]
PfamiPF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.

Sequencei

Sequence statusi: Complete.

P40555-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEEELSKLL ANVKIDPSLT SRISQIDSFK LSELMVLKTD IETQLEAYFS
60 70 80 90 100
VLEQQGIGMD SALVTPDGYP RSDVDVLQVT MIRKNVNMLK NDLNHLLQRS
110 120 130 140 150
HVLLNQHFDN MNVKSNQDAR RNNDDQAIQY TIPFAFISEV VPGSPSDKAD
160 170 180 190 200
IKVDDKLISI GNVHAANHSK LQNIQMVVMK NEDRPLPVLL LREGQILKTS
210 220
LTPSRNWNGR GLLGCRIQEL
Length:220
Mass (Da):24,846
Last modified:February 1, 1995 - v1
Checksum:i1BA966C5B7B2D02B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38113 Genomic DNA. Translation: CAA86244.1.
AY558294 Genomic DNA. Translation: AAS56620.1.
BK006942 Genomic DNA. Translation: DAA08539.1.
PIRiS48450.
RefSeqiNP_012259.3. NM_001179357.3.

Genome annotation databases

EnsemblFungiiYIL007C; YIL007C; YIL007C.
GeneIDi854810.
KEGGisce:YIL007C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38113 Genomic DNA. Translation: CAA86244.1.
AY558294 Genomic DNA. Translation: AAS56620.1.
BK006942 Genomic DNA. Translation: DAA08539.1.
PIRiS48450.
RefSeqiNP_012259.3. NM_001179357.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3WHJX-ray1.65A1-120[»]
3WHLX-ray4.00B/D/F/H1-120[»]
4O06X-ray1.15A126-220[»]
ProteinModelPortaliP40555.
SMRiP40555. Positions 1-117, 129-220.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34985. 31 interactions.
DIPiDIP-1893N.
IntActiP40555. 10 interactions.
MINTiMINT-394729.

Proteomic databases

MaxQBiP40555.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL007C; YIL007C; YIL007C.
GeneIDi854810.
KEGGisce:YIL007C.

Organism-specific databases

EuPathDBiFungiDB:YIL007C.
SGDiS000001269. NAS2.

Phylogenomic databases

GeneTreeiENSGT00390000004147.
HOGENOMiHOG000216665.
InParanoidiP40555.
KOiK06693.
OMAiLGCNIVP.
OrthoDBiEOG7V1G2H.

Enzyme and pathway databases

BioCyciYEAST:G3O-31286-MONOMER.
ReactomeiR-SCE-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-SCE-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SCE-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-SCE-5668541. TNFR2 non-canonical NF-kB pathway.
R-SCE-5687128. MAPK6/MAPK4 signaling.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SCE-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SCE-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiP40555.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
[Graphical view]
PfamiPF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "cDNA cloning and characterization of a human proteasomal modulator subunit, p27 (PSMD9)."
    Watanabe T.K., Saito A., Suzuki M., Fujiwara T., Takahashi E., Slaughter C.A., DeMartino G.N., Hendil K.B., Chung C.H., Tanahashi N., Tanaka K.
    Genomics 50:241-250(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Multiple assembly chaperones govern biogenesis of the proteasome regulatory particle base."
    Funakoshi M., Tomko R.J. Jr., Kobayashi H., Hochstrasser M.
    Cell 137:887-899(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS PROTEASOME CHAPERONE.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPSMD9_YEAST
AccessioniPrimary (citable) accession number: P40555
Secondary accession number(s): D6VVS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 6, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 846 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.