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P40549 (MNT3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-1,3-mannosyltransferase MNT3

EC=2.4.1.-
Gene names
Name:MNT3
Ordered Locus Names:YIL014W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length630 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein Potential.

Miscellaneous

Present with 2310 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the MNN1/MNT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 630630Alpha-1,3-mannosyltransferase MNT3
PRO_0000080560

Regions

Topological domain1 – 1414Cytoplasmic Potential
Transmembrane15 – 3117Helical; Signal-anchor for type II membrane protein; Potential
Topological domain32 – 630599Lumenal Potential

Amino acid modifications

Glycosylation341N-linked (GlcNAc...) Potential
Glycosylation1681N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P40549 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 6B087DD003D8CD91

FASTA63072,410
        10         20         30         40         50         60 
MLKSLKSRRL ILKRLVTLLL SLFFSYLIFS ASRNVTSSNK LNNHASERTA VESSAFNWIE 

        70         80         90        100        110        120 
KRQHQVRSEN LMNRLSAYFL PFLSRSSHKE RVLLRQLGNN EIAKSDKCRY IFEVLYKIDP 

       130        140        150        160        170        180 
DWDNAQTAKF YNVDGVDNTL ASLLGERLRS YDYCFLSGQL DPTAIFANST VNPHDLQNRM 

       190        200        210        220        230        240 
FPFLKKINEE SKTVMWPIIT DMTTGEAVPA PEVDMESSNF NGNFWSNWNR LSKGRGFVLT 

       250        260        270        280        290        300 
IAEKDVPLFL KQLKVMEFSK NELPFQIVST GNELSAESIA KISETAKETE QRVYLVDCST 

       310        320        330        340        350        360 
VLDTNFANTY ISFFQNKWVA TLFNTFEEYI LLDADVVPFV GSDYFFDSPS YRESGILLFK 

       370        380        390        400        410        420 
DRVMENEQTF QYCIEMLNEV EPSAQERRFI GSRLVFDSSL PFSSETSEEA SVYYNFFKKL 

       430        440        450        460        470        480 
RLHHVDSGLV VVNKLEKLNG LLMSFMLNLD GKLQRCVYGD KEIFWLGQLY AGQDYSINPV 

       490        500        510        520        530        540 
DGSIIGPVNE EPENDDGHKS GMYYICSTQI AHSDSKNRLL WVNGGLKTCK ISNSAEDDFG 

       550        560        570        580        590        600 
REPEYFKSRY GDISKLKRIY DASLNVEGLI VPDVSVHPWM QIKECSNYMY CAYATGDGHT 

       610        620        630 
NSELDEGRLI TFTEKELRYI NDISRTWNAN 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Mnt2p and Mnt3p of Saccharomyces cerevisiae are members of the Mnn1p family of alpha-1,3-mannosyltransferases responsible for adding the terminal mannose residues of O-linked oligosaccharides."
Romero P.A., Lussier M., Veronneau S., Sdicu A.-M., Herscovics A., Bussey H.
Glycobiology 9:1045-1051(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46881 Genomic DNA. Translation: CAA86979.1.
AY558107 Genomic DNA. Translation: AAS56433.1.
BK006942 Genomic DNA. Translation: DAA08532.1.
PIRS49969.
RefSeqNP_012251.3. NM_001179364.3.

3D structure databases

ProteinModelPortalP40549.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34977. 12 interactions.
DIPDIP-4122N.
IntActP40549. 1 interaction.
MINTMINT-548877.
STRING4932.YIL014W.

Protein family/group databases

CAZyGT71. Glycosyltransferase Family 71.

Proteomic databases

MaxQBP40549.
PaxDbP40549.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIL014W; YIL014W; YIL014W.
GeneID854801.
KEGGsce:YIL014W.

Organism-specific databases

CYGDYIL014w.
SGDS000001276. MNT3.

Phylogenomic databases

eggNOGNOG127370.
GeneTreeENSGT00390000003622.
HOGENOMHOG000113621.
KOK10969.
OMAWVNGGLK.
OrthoDBEOG7QRR3H.

Enzyme and pathway databases

BioCycYEAST:G3O-31290-MONOMER.
UniPathwayUPA00378.

Gene expression databases

GenevestigatorP40549.

Family and domain databases

InterProIPR022751. Alpha_mannosyltransferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamPF11051. Mannosyl_trans3. 1 hit.
[Graphical view]
SUPFAMSSF53448. SSF53448. 2 hits.
ProtoNetSearch...

Other

NextBio977613.

Entry information

Entry nameMNT3_YEAST
AccessionPrimary (citable) accession number: P40549
Secondary accession number(s): D6VVR6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways