P40549 (MNT3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,3-mannosyltransferase MNT3
EC=2.4.1.-

Gene names

Name:	MNT3
Ordered Locus Names:	YIL014W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	630 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Miscellaneous	Present with 2310 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the MNN1/MNT family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein O-linked glycosylation Inferred from direct assay Ref.4. Source: SGD
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	alpha-1,3-mannosyltransferase activity Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 630	630	Alpha-1,3-mannosyltransferase MNT3		PRO_0000080560
Regions
	Topological domain	1 – 14	14	Cytoplasmic Potential
	Transmembrane	15 – 31	17	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	32 – 630	599	Lumenal Potential
Amino acid modifications
	Glycosylation	34	1	N-linked (GlcNAc...) Potential
	Glycosylation	168	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P40549 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 6B087DD003D8CD91	FASTA	630	72,410
	10 20 30 40 50 60 MLKSLKSRRL ILKRLVTLLL SLFFSYLIFS ASRNVTSSNK LNNHASERTA VESSAFNWIE 70 80 90 100 110 120 KRQHQVRSEN LMNRLSAYFL PFLSRSSHKE RVLLRQLGNN EIAKSDKCRY IFEVLYKIDP 130 140 150 160 170 180 DWDNAQTAKF YNVDGVDNTL ASLLGERLRS YDYCFLSGQL DPTAIFANST VNPHDLQNRM 190 200 210 220 230 240 FPFLKKINEE SKTVMWPIIT DMTTGEAVPA PEVDMESSNF NGNFWSNWNR LSKGRGFVLT 250 260 270 280 290 300 IAEKDVPLFL KQLKVMEFSK NELPFQIVST GNELSAESIA KISETAKETE QRVYLVDCST 310 320 330 340 350 360 VLDTNFANTY ISFFQNKWVA TLFNTFEEYI LLDADVVPFV GSDYFFDSPS YRESGILLFK 370 380 390 400 410 420 DRVMENEQTF QYCIEMLNEV EPSAQERRFI GSRLVFDSSL PFSSETSEEA SVYYNFFKKL 430 440 450 460 470 480 RLHHVDSGLV VVNKLEKLNG LLMSFMLNLD GKLQRCVYGD KEIFWLGQLY AGQDYSINPV 490 500 510 520 530 540 DGSIIGPVNE EPENDDGHKS GMYYICSTQI AHSDSKNRLL WVNGGLKTCK ISNSAEDDFG 550 560 570 580 590 600 REPEYFKSRY GDISKLKRIY DASLNVEGLI VPDVSVHPWM QIKECSNYMY CAYATGDGHT 610 620 630 NSELDEGRLI TFTEKELRYI NDISRTWNAN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Mnt2p and Mnt3p of Saccharomyces cerevisiae are members of the Mnn1p family of alpha-1,3-mannosyltransferases responsible for adding the terminal mannose residues of O-linked oligosaccharides." Romero P.A., Lussier M., Veronneau S., Sdicu A.-M., Herscovics A., Bussey H. Glycobiology 9:1045-1051(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46881 Genomic DNA. Translation: CAA86979.1. AY558107 Genomic DNA. Translation: AAS56433.1. BK006942 Genomic DNA. Translation: DAA08532.1.
PIR	S49969.
RefSeq	NP_012251.3. NM_001179364.3.
3D structure databases
ProteinModelPortal	P40549.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4122N.
IntAct	P40549. 1 interaction.
MINT	MINT-548877.
STRING	4932.YIL014W.
Protein family/group databases
CAZy	GT71. Glycosyltransferase Family 71.
Proteomic databases
PaxDb	P40549.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL014W; YIL014W; YIL014W.
GeneID	854801.
KEGG	sce:YIL007C. sce:YIL014W.
Organism-specific databases
CYGD	YIL014w.
SGD	S000001276. MNT3.
Phylogenomic databases
eggNOG	NOG127370.
GeneTree	ENSGT00390000003622.
HOGENOM	HOG000113621.
KO	K06693. K10969.
OMA	EIFWLGQ.
OrthoDB	EOG4004SR.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
Genevestigator	P40549.
GermOnline	YIL014W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022751. Alpha_mannosyltransferase. [Graphical view]
Pfam	PF11051. Mannosyl_trans3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977613.

Entry information

Entry name

MNT3_YEAST

Accession

Primary (citable) accession number: P40549
Secondary accession number(s): D6VVR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program