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P40549

- MNT3_YEAST

UniProt

P40549 - MNT3_YEAST

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Protein
Alpha-1,3-mannosyltransferase MNT3
Gene
MNT3, YIL014W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans.

Pathwayi

GO - Molecular functioni

  1. alpha-1,3-mannosyltransferase activity Source: SGD

GO - Biological processi

  1. mannosylation Source: GOC
  2. protein O-linked glycosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-31290-MONOMER.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT71. Glycosyltransferase Family 71.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-mannosyltransferase MNT3 (EC:2.4.1.-)
Gene namesi
Name:MNT3
Ordered Locus Names:YIL014W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIL014w.
SGDiS000001276. MNT3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1414Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei15 – 3117Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini32 – 630599Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: SGD
  2. Golgi membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 630630Alpha-1,3-mannosyltransferase MNT3
PRO_0000080560Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi34 – 341N-linked (GlcNAc...) Reviewed prediction
Glycosylationi168 – 1681N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP40549.
PaxDbiP40549.

Expressioni

Gene expression databases

GenevestigatoriP40549.

Interactioni

Protein-protein interaction databases

BioGridi34977. 12 interactions.
DIPiDIP-4122N.
IntActiP40549. 1 interaction.
MINTiMINT-548877.
STRINGi4932.YIL014W.

Structurei

3D structure databases

ProteinModelPortaliP40549.

Family & Domainsi

Sequence similaritiesi

Belongs to the MNN1/MNT family.

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG127370.
GeneTreeiENSGT00390000003622.
HOGENOMiHOG000113621.
KOiK10969.
OMAiWVNGGLK.
OrthoDBiEOG7QRR3H.

Family and domain databases

InterProiIPR022751. Alpha_mannosyltransferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF11051. Mannosyl_trans3. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

P40549-1 [UniParc]FASTAAdd to Basket

« Hide

MLKSLKSRRL ILKRLVTLLL SLFFSYLIFS ASRNVTSSNK LNNHASERTA    50
VESSAFNWIE KRQHQVRSEN LMNRLSAYFL PFLSRSSHKE RVLLRQLGNN 100
EIAKSDKCRY IFEVLYKIDP DWDNAQTAKF YNVDGVDNTL ASLLGERLRS 150
YDYCFLSGQL DPTAIFANST VNPHDLQNRM FPFLKKINEE SKTVMWPIIT 200
DMTTGEAVPA PEVDMESSNF NGNFWSNWNR LSKGRGFVLT IAEKDVPLFL 250
KQLKVMEFSK NELPFQIVST GNELSAESIA KISETAKETE QRVYLVDCST 300
VLDTNFANTY ISFFQNKWVA TLFNTFEEYI LLDADVVPFV GSDYFFDSPS 350
YRESGILLFK DRVMENEQTF QYCIEMLNEV EPSAQERRFI GSRLVFDSSL 400
PFSSETSEEA SVYYNFFKKL RLHHVDSGLV VVNKLEKLNG LLMSFMLNLD 450
GKLQRCVYGD KEIFWLGQLY AGQDYSINPV DGSIIGPVNE EPENDDGHKS 500
GMYYICSTQI AHSDSKNRLL WVNGGLKTCK ISNSAEDDFG REPEYFKSRY 550
GDISKLKRIY DASLNVEGLI VPDVSVHPWM QIKECSNYMY CAYATGDGHT 600
NSELDEGRLI TFTEKELRYI NDISRTWNAN 630
Length:630
Mass (Da):72,410
Last modified:February 1, 1995 - v1
Checksum:i6B087DD003D8CD91
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46881 Genomic DNA. Translation: CAA86979.1.
AY558107 Genomic DNA. Translation: AAS56433.1.
BK006942 Genomic DNA. Translation: DAA08532.1.
PIRiS49969.
RefSeqiNP_012251.3. NM_001179364.3.

Genome annotation databases

EnsemblFungiiYIL014W; YIL014W; YIL014W.
GeneIDi854801.
KEGGisce:YIL014W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46881 Genomic DNA. Translation: CAA86979.1 .
AY558107 Genomic DNA. Translation: AAS56433.1 .
BK006942 Genomic DNA. Translation: DAA08532.1 .
PIRi S49969.
RefSeqi NP_012251.3. NM_001179364.3.

3D structure databases

ProteinModelPortali P40549.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34977. 12 interactions.
DIPi DIP-4122N.
IntActi P40549. 1 interaction.
MINTi MINT-548877.
STRINGi 4932.YIL014W.

Protein family/group databases

CAZyi GT71. Glycosyltransferase Family 71.

Proteomic databases

MaxQBi P40549.
PaxDbi P40549.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIL014W ; YIL014W ; YIL014W .
GeneIDi 854801.
KEGGi sce:YIL014W.

Organism-specific databases

CYGDi YIL014w.
SGDi S000001276. MNT3.

Phylogenomic databases

eggNOGi NOG127370.
GeneTreei ENSGT00390000003622.
HOGENOMi HOG000113621.
KOi K10969.
OMAi WVNGGLK.
OrthoDBi EOG7QRR3H.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BioCyci YEAST:G3O-31290-MONOMER.

Miscellaneous databases

NextBioi 977613.

Gene expression databases

Genevestigatori P40549.

Family and domain databases

InterProi IPR022751. Alpha_mannosyltransferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
Pfami PF11051. Mannosyl_trans3. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Mnt2p and Mnt3p of Saccharomyces cerevisiae are members of the Mnn1p family of alpha-1,3-mannosyltransferases responsible for adding the terminal mannose residues of O-linked oligosaccharides."
    Romero P.A., Lussier M., Veronneau S., Sdicu A.-M., Herscovics A., Bussey H.
    Glycobiology 9:1045-1051(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMNT3_YEAST
AccessioniPrimary (citable) accession number: P40549
Secondary accession number(s): D6VVR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2310 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

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