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P40549

- MNT3_YEAST

UniProt

P40549 - MNT3_YEAST

Protein

Alpha-1,3-mannosyltransferase MNT3

Gene

MNT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans.

    Pathwayi

    GO - Molecular functioni

    1. alpha-1,3-mannosyltransferase activity Source: SGD

    GO - Biological processi

    1. mannosylation Source: GOC
    2. protein O-linked glycosylation Source: SGD

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31290-MONOMER.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT71. Glycosyltransferase Family 71.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-1,3-mannosyltransferase MNT3 (EC:2.4.1.-)
    Gene namesi
    Name:MNT3
    Ordered Locus Names:YIL014W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL014w.
    SGDiS000001276. MNT3.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi apparatus Source: SGD
    2. Golgi membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 630630Alpha-1,3-mannosyltransferase MNT3PRO_0000080560Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP40549.
    PaxDbiP40549.

    Expressioni

    Gene expression databases

    GenevestigatoriP40549.

    Interactioni

    Protein-protein interaction databases

    BioGridi34977. 12 interactions.
    DIPiDIP-4122N.
    IntActiP40549. 1 interaction.
    MINTiMINT-548877.
    STRINGi4932.YIL014W.

    Structurei

    3D structure databases

    ProteinModelPortaliP40549.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1414CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini32 – 630599LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei15 – 3117Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MNN1/MNT family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG127370.
    GeneTreeiENSGT00390000003622.
    HOGENOMiHOG000113621.
    KOiK10969.
    OMAiWVNGGLK.
    OrthoDBiEOG7QRR3H.

    Family and domain databases

    InterProiIPR022751. Alpha_mannosyltransferase.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PfamiPF11051. Mannosyl_trans3. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P40549-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKSLKSRRL ILKRLVTLLL SLFFSYLIFS ASRNVTSSNK LNNHASERTA    50
    VESSAFNWIE KRQHQVRSEN LMNRLSAYFL PFLSRSSHKE RVLLRQLGNN 100
    EIAKSDKCRY IFEVLYKIDP DWDNAQTAKF YNVDGVDNTL ASLLGERLRS 150
    YDYCFLSGQL DPTAIFANST VNPHDLQNRM FPFLKKINEE SKTVMWPIIT 200
    DMTTGEAVPA PEVDMESSNF NGNFWSNWNR LSKGRGFVLT IAEKDVPLFL 250
    KQLKVMEFSK NELPFQIVST GNELSAESIA KISETAKETE QRVYLVDCST 300
    VLDTNFANTY ISFFQNKWVA TLFNTFEEYI LLDADVVPFV GSDYFFDSPS 350
    YRESGILLFK DRVMENEQTF QYCIEMLNEV EPSAQERRFI GSRLVFDSSL 400
    PFSSETSEEA SVYYNFFKKL RLHHVDSGLV VVNKLEKLNG LLMSFMLNLD 450
    GKLQRCVYGD KEIFWLGQLY AGQDYSINPV DGSIIGPVNE EPENDDGHKS 500
    GMYYICSTQI AHSDSKNRLL WVNGGLKTCK ISNSAEDDFG REPEYFKSRY 550
    GDISKLKRIY DASLNVEGLI VPDVSVHPWM QIKECSNYMY CAYATGDGHT 600
    NSELDEGRLI TFTEKELRYI NDISRTWNAN 630
    Length:630
    Mass (Da):72,410
    Last modified:February 1, 1995 - v1
    Checksum:i6B087DD003D8CD91
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46881 Genomic DNA. Translation: CAA86979.1.
    AY558107 Genomic DNA. Translation: AAS56433.1.
    BK006942 Genomic DNA. Translation: DAA08532.1.
    PIRiS49969.
    RefSeqiNP_012251.3. NM_001179364.3.

    Genome annotation databases

    EnsemblFungiiYIL014W; YIL014W; YIL014W.
    GeneIDi854801.
    KEGGisce:YIL014W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46881 Genomic DNA. Translation: CAA86979.1 .
    AY558107 Genomic DNA. Translation: AAS56433.1 .
    BK006942 Genomic DNA. Translation: DAA08532.1 .
    PIRi S49969.
    RefSeqi NP_012251.3. NM_001179364.3.

    3D structure databases

    ProteinModelPortali P40549.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34977. 12 interactions.
    DIPi DIP-4122N.
    IntActi P40549. 1 interaction.
    MINTi MINT-548877.
    STRINGi 4932.YIL014W.

    Protein family/group databases

    CAZyi GT71. Glycosyltransferase Family 71.

    Proteomic databases

    MaxQBi P40549.
    PaxDbi P40549.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL014W ; YIL014W ; YIL014W .
    GeneIDi 854801.
    KEGGi sce:YIL014W.

    Organism-specific databases

    CYGDi YIL014w.
    SGDi S000001276. MNT3.

    Phylogenomic databases

    eggNOGi NOG127370.
    GeneTreei ENSGT00390000003622.
    HOGENOMi HOG000113621.
    KOi K10969.
    OMAi WVNGGLK.
    OrthoDBi EOG7QRR3H.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BioCyci YEAST:G3O-31290-MONOMER.

    Miscellaneous databases

    NextBioi 977613.

    Gene expression databases

    Genevestigatori P40549.

    Family and domain databases

    InterProi IPR022751. Alpha_mannosyltransferase.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    Pfami PF11051. Mannosyl_trans3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Mnt2p and Mnt3p of Saccharomyces cerevisiae are members of the Mnn1p family of alpha-1,3-mannosyltransferases responsible for adding the terminal mannose residues of O-linked oligosaccharides."
      Romero P.A., Lussier M., Veronneau S., Sdicu A.-M., Herscovics A., Bussey H.
      Glycobiology 9:1045-1051(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMNT3_YEAST
    AccessioniPrimary (citable) accession number: P40549
    Secondary accession number(s): D6VVR6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2310 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3