P40548 (SNL1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HSP70 co-chaperone SNL1

Alternative name(s):
Suppressor of NUP116-C lethal

Gene names

Name:	SNL1
Ordered Locus Names:	YIL016W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	159 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stimulator of ATPase activity of molecular chaperones of the HSP70 family (principally of the SSA class). Stimulation is important for HSP70-substrate complex dissociation after folding of newly synthesized or refolded proteins. SNL1 is probably involved in nuclear pore biogenesis and in particular the folding or refolding of misfolded NUP116, GLE2 and NIC96. Ref.4 Ref.5
Subunit structure	Interacts with the HSP70 family members SSA1, SSA4, and SSB1. These interactions are strongly reduced by ADP and ATP. Ref.5
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein. Nucleus membrane; Single-pass type II membrane protein Ref.4.
Miscellaneous	Present with 3346 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 BAG domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Nucleus
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nuclear pore organization Inferred from genetic interaction Ref.4. Source: SGD protein folding Inferred from physical interaction Ref.5. Source: SGD
Cellular_component	endoplasmic reticulum membrane Inferred from direct assay Ref.4. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrion Inferred from direct assay PubMed 14576278PubMed 16823961. Source: SGD nuclear envelope Inferred from direct assay Ref.4. Source: SGD nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ribosome binding Inferred from direct assay PubMed 22635919. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 159	159	HSP70 co-chaperone SNL1		PRO_0000088881
Regions
	Topological domain	1 – 12	12	Perinuclear space
	Transmembrane	13 – 35	23	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	36 – 159	124	Cytoplasmic
	Domain	73 – 159	87	BAG
	Compositional bias	42 – 63	22	Lys-rich
Experimental info
	Mutagenesis	112	1	E → A: Strongly reduces interaction with HSP70 family members. Ref.5
	Mutagenesis	141	1	R → A: Strongly reduces interaction with HSP70 family members. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P40548 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 05524B473D3E888E	FASTA	159	18,306
	10 20 30 40 50 60 MSHNAMEHWK SKLSKTSTST YVLLAVIAVV FLVTIRRPNG SKGKSSKKRA SKKNKKGKNQ 70 80 90 100 110 120 FEKAPVPLTL EEQIDNVSLR YGNELEGRSK DLINRFDVED EKDIYERNYC NEMLLKLLIE 130 140 150 LDSIDLINVD ESLRRPLKEK RKGVIKEIQA MLKSLDSLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The integral membrane protein snl1p is genetically linked to yeast nuclear pore complex function." Ho A.K., Raczniak G.A., Ives E.B., Wente S.R. Mol. Biol. Cell 9:355-373(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, NUCLEAR PORE ASSEMBLY.
[5]	"Prediction of novel Bag-1 homologs based on structure/function analysis identifies Snl1p as an Hsp70 co-chaperone in Saccharomyces cerevisiae." Sondermann H., Ho A.K., Listenberger L.L., Siegers K., Moarefi I., Wente S.R., Hartl F.-U., Young J.C. J. Biol. Chem. 277:33220-33227(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SSA1; SSA4 AND SSB1, HSP70 CO-CHAPERONE ACTIVITY, MUTAGENESIS OF GLU-112 AND ARG-141.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46881 Genomic DNA. Translation: CAA86976.1. AY558552 Genomic DNA. Translation: AAS56878.1. BK006942 Genomic DNA. Translation: DAA08529.1.
PIR	S49966.
RefSeq	NP_012248.1. NM_001179366.1.
3D structure databases
ProteinModelPortal	P40548.
SMR	P40548. Positions 73-157.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5636N.
IntAct	P40548. 30 interactions.
MINT	MINT-552100.
STRING	4932.YIL016W.
Protein family/group databases
TCDB	9.A.14.1.1. nuclear pore complex (NPC) family.
Proteomic databases
PaxDb	P40548.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL016W; YIL016W; YIL016W.
GeneID	854796.
KEGG	sce:YIL016W.
Organism-specific databases
CYGD	YIL016w.
SGD	S000001278. SNL1.
Phylogenomic databases
eggNOG	NOG77307.
HOGENOM	HOG000000972.
KO	K14017.
OMA	CNEMLLK.
OrthoDB	EOG4Z0FFN.
Gene expression databases
Genevestigator	P40548.
GermOnline	YIL016W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003103. BAG_domain. [Graphical view]
Pfam	PF02179. BAG. 1 hit. [Graphical view]
SMART	SM00264. BAG. 1 hit. [Graphical view]
PROSITE	PS51035. BAG. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977600.

Entry information

Entry name

SNL1_YEAST

Accession

Primary (citable) accession number: P40548
Secondary accession number(s): D6VVR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program