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P40548 (SNL1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
HSP70 co-chaperone SNL1
Alternative name(s):
Suppressor of NUP116-C lethal
Gene names
Name:SNL1
Ordered Locus Names:YIL016W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length159 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stimulator of ATPase activity of molecular chaperones of the HSP70 family (principally of the SSA class). Stimulation is important for HSP70-substrate complex dissociation after folding of newly synthesized or refolded proteins. SNL1 is probably involved in nuclear pore biogenesis and in particular the folding or refolding of misfolded NUP116, GLE2 and NIC96. Ref.4 Ref.5

Subunit structure

Interacts with the HSP70 family members SSA1, SSA4, and SSB1. These interactions are strongly reduced by ADP and ATP. Ref.5

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein. Nucleus membrane; Single-pass type II membrane protein Ref.4.

Miscellaneous

Present with 3346 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 BAG domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 159159HSP70 co-chaperone SNL1
PRO_0000088881

Regions

Topological domain1 – 1212Perinuclear space
Transmembrane13 – 3523Helical; Signal-anchor for type II membrane protein; Potential
Topological domain36 – 159124Cytoplasmic
Domain73 – 15987BAG
Compositional bias42 – 6322Lys-rich

Experimental info

Mutagenesis1121E → A: Strongly reduces interaction with HSP70 family members. Ref.5
Mutagenesis1411R → A: Strongly reduces interaction with HSP70 family members. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P40548 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 05524B473D3E888E

FASTA15918,306
        10         20         30         40         50         60 
MSHNAMEHWK SKLSKTSTST YVLLAVIAVV FLVTIRRPNG SKGKSSKKRA SKKNKKGKNQ 

        70         80         90        100        110        120 
FEKAPVPLTL EEQIDNVSLR YGNELEGRSK DLINRFDVED EKDIYERNYC NEMLLKLLIE 

       130        140        150 
LDSIDLINVD ESLRRPLKEK RKGVIKEIQA MLKSLDSLK 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The integral membrane protein snl1p is genetically linked to yeast nuclear pore complex function."
Ho A.K., Raczniak G.A., Ives E.B., Wente S.R.
Mol. Biol. Cell 9:355-373(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, NUCLEAR PORE ASSEMBLY.
[5]"Prediction of novel Bag-1 homologs based on structure/function analysis identifies Snl1p as an Hsp70 co-chaperone in Saccharomyces cerevisiae."
Sondermann H., Ho A.K., Listenberger L.L., Siegers K., Moarefi I., Wente S.R., Hartl F.-U., Young J.C.
J. Biol. Chem. 277:33220-33227(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SSA1; SSA4 AND SSB1, HSP70 CO-CHAPERONE ACTIVITY, MUTAGENESIS OF GLU-112 AND ARG-141.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46881 Genomic DNA. Translation: CAA86976.1.
AY558552 Genomic DNA. Translation: AAS56878.1.
BK006942 Genomic DNA. Translation: DAA08529.1.
PIRS49966.
RefSeqNP_012248.1. NM_001179366.1.

3D structure databases

ProteinModelPortalP40548.
SMRP40548. Positions 73-159.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34972. 108 interactions.
DIPDIP-5636N.
IntActP40548. 41 interactions.
MINTMINT-552100.
STRING4932.YIL016W.

Protein family/group databases

TCDB1.I.1.1.1. the nuclear pore complex (npc) family.

Proteomic databases

PaxDbP40548.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIL016W; YIL016W; YIL016W.
GeneID854796.
KEGGsce:YIL016W.

Organism-specific databases

CYGDYIL016w.
SGDS000001278. SNL1.

Phylogenomic databases

eggNOGNOG77307.
HOGENOMHOG000000972.
KOK14017.
OMACNEMLLK.
OrthoDBEOG78M0F4.

Enzyme and pathway databases

BioCycYEAST:G3O-31292-MONOMER.

Gene expression databases

GenevestigatorP40548.

Family and domain databases

Gene3D1.20.58.120. 1 hit.
InterProIPR003103. BAG_domain.
[Graphical view]
PfamPF02179. BAG. 1 hit.
[Graphical view]
SMARTSM00264. BAG. 1 hit.
[Graphical view]
PROSITEPS51035. BAG. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977600.

Entry information

Entry nameSNL1_YEAST
AccessionPrimary (citable) accession number: P40548
Secondary accession number(s): D6VVR3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families