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P40545

- HIS4_YEAST

UniProt

P40545 - HIS4_YEAST

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Protein
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
Gene
HIS6, YIL020C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of the aminoaldose moiety of ProFAR to the aminoketose of PRFAR.

Catalytic activityi

1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide.

Pathwayi

GO - Molecular functioni

  1. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity Source: SGD

GO - Biological processi

  1. histidine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YIL020C-MONOMER.
BRENDAi5.3.1.16. 984.
UniPathwayiUPA00031; UER00009.

Names & Taxonomyi

Protein namesi
Recommended name:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (EC:5.3.1.16)
Alternative name(s):
5-proFAR isomerase
Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase
Gene namesi
Name:HIS6
Ordered Locus Names:YIL020C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIL020c.
SGDiS000001282. HIS6.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2612611-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
PRO_0000141963Add
BLAST

Proteomic databases

MaxQBiP40545.
PaxDbiP40545.
PeptideAtlasiP40545.

Expressioni

Gene expression databases

GenevestigatoriP40545.

Interactioni

Protein-protein interaction databases

BioGridi34968. 16 interactions.
DIPiDIP-4716N.
IntActiP40545. 1 interaction.
MINTiMINT-561085.
STRINGi4932.YIL020C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 115
Helixi41 – 5010
Beta strandi57 – 648
Helixi66 – 7510
Turni77 – 793
Beta strandi80 – 856
Turni88 – 903
Helixi91 – 944
Turni95 – 973
Beta strandi101 – 1033
Helixi105 – 1073
Helixi116 – 12611
Helixi128 – 1303
Beta strandi131 – 14111
Beta strandi144 – 1496
Turni150 – 1534
Beta strandi154 – 1618
Helixi162 – 1687
Turni169 – 1713
Beta strandi173 – 1786
Helixi191 – 20111
Beta strandi208 – 2136
Helixi220 – 2289
Beta strandi232 – 2354
Helixi241 – 2433
Beta strandi246 – 2483
Helixi250 – 26011

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AGKX-ray1.30A2-261[»]
ProteinModelPortaliP40545.
SMRiP40545. Positions 2-261.

Miscellaneous databases

EvolutionaryTraceiP40545.

Family & Domainsi

Sequence similaritiesi

Belongs to the HisA/HisF family.

Phylogenomic databases

eggNOGiCOG0106.
HOGENOMiHOG000172261.
KOiK01814.
OMAiFVAMNKW.
OrthoDBiEOG7ZWDC9.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006062. His_biosynth.
IPR011858. HisA_euk.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PANTHERiPTHR21169. PTHR21169. 1 hit.
PfamiPF00977. His_biosynth. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR02129. hisA_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

P40545-1 [UniParc]FASTAAdd to Basket

« Hide

MTKFIGCIDL HNGEVKQIVG GTLTSKKEDV PKTNFVSQHP SSYYAKLYKD    50
RDVQGCHVIK LGPNNDDAAR EALQESPQFL QVGGGINDTN CLEWLKWASK 100
VIVTSWLFTK EGHFQLKRLE RLTELCGKDR IVVDLSCRKT QDGRWIVAMN 150
KWQTLTDLEL NADTFRELRK YTNEFLIHAA DVEGLCGGID ELLVSKLFEW 200
TKDYDDLKIV YAGGAKSVDD LKLVDELSHG KVDLTFGSSL DIFGGNLVKF 250
EDCCRWNEKQ G 261
Length:261
Mass (Da):29,557
Last modified:February 1, 1995 - v1
Checksum:iFA33F3A8ED52B526
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti243 – 2431F → S in AAS56185. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87341 Genomic DNA. Translation: CAA60779.1.
Z46881 Genomic DNA. Translation: CAA86972.1.
AY557859 Genomic DNA. Translation: AAS56185.1.
BK006942 Genomic DNA. Translation: DAA08525.1.
PIRiS49962.
RefSeqiNP_012244.3. NM_001179370.3.

Genome annotation databases

EnsemblFungiiYIL020C; YIL020C; YIL020C.
GeneIDi854792.
KEGGisce:YIL020C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87341 Genomic DNA. Translation: CAA60779.1 .
Z46881 Genomic DNA. Translation: CAA86972.1 .
AY557859 Genomic DNA. Translation: AAS56185.1 .
BK006942 Genomic DNA. Translation: DAA08525.1 .
PIRi S49962.
RefSeqi NP_012244.3. NM_001179370.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2AGK X-ray 1.30 A 2-261 [» ]
ProteinModelPortali P40545.
SMRi P40545. Positions 2-261.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34968. 16 interactions.
DIPi DIP-4716N.
IntActi P40545. 1 interaction.
MINTi MINT-561085.
STRINGi 4932.YIL020C.

Proteomic databases

MaxQBi P40545.
PaxDbi P40545.
PeptideAtlasi P40545.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIL020C ; YIL020C ; YIL020C .
GeneIDi 854792.
KEGGi sce:YIL020C.

Organism-specific databases

CYGDi YIL020c.
SGDi S000001282. HIS6.

Phylogenomic databases

eggNOGi COG0106.
HOGENOMi HOG000172261.
KOi K01814.
OMAi FVAMNKW.
OrthoDBi EOG7ZWDC9.

Enzyme and pathway databases

UniPathwayi UPA00031 ; UER00009 .
BioCyci YEAST:YIL020C-MONOMER.
BRENDAi 5.3.1.16. 984.

Miscellaneous databases

EvolutionaryTracei P40545.
NextBioi 977588.

Gene expression databases

Genevestigatori P40545.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR006062. His_biosynth.
IPR011858. HisA_euk.
IPR011060. RibuloseP-bd_barrel.
[Graphical view ]
PANTHERi PTHR21169. PTHR21169. 1 hit.
Pfami PF00977. His_biosynth. 1 hit.
[Graphical view ]
SUPFAMi SSF51366. SSF51366. 1 hit.
TIGRFAMsi TIGR02129. hisA_euk. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Paralogous histidine biosynthetic genes: evolutionary analysis of the Saccharomyces cerevisiae HIS6 and HIS7 genes."
    Fani R., Tamburini E., Mori E., Lazcano A., Lio P., Barberio C., Casalone E., Cavalieri D., Perito B., Polsinelli M.
    Gene 197:9-17(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: YNN 282.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Crystal structure of the yeast His6 enzyme suggests a reaction mechanism."
    Quevillon-Cheruel S., Leulliot N., Graille M., Blondeau K., Janin J., van Tilbeurgh H.
    Protein Sci. 15:1516-1521(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS) OF 2-261.

Entry informationi

Entry nameiHIS4_YEAST
AccessioniPrimary (citable) accession number: P40545
Secondary accession number(s): D6VVQ9, Q6Q5P5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 14, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6490 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

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