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P40545

- HIS4_YEAST

UniProt

P40545 - HIS4_YEAST

Protein

1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Gene

HIS6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the isomerization of the aminoaldose moiety of ProFAR to the aminoketose of PRFAR.

    Catalytic activityi

    1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide.

    Pathwayi

    GO - Molecular functioni

    1. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity Source: SGD

    GO - Biological processi

    1. histidine biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Histidine biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:YIL020C-MONOMER.
    BRENDAi5.3.1.16. 984.
    UniPathwayiUPA00031; UER00009.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (EC:5.3.1.16)
    Alternative name(s):
    5-proFAR isomerase
    Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase
    Gene namesi
    Name:HIS6
    Ordered Locus Names:YIL020C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL020c.
    SGDiS000001282. HIS6.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2612611-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerasePRO_0000141963Add
    BLAST

    Proteomic databases

    MaxQBiP40545.
    PaxDbiP40545.
    PeptideAtlasiP40545.

    Expressioni

    Gene expression databases

    GenevestigatoriP40545.

    Interactioni

    Protein-protein interaction databases

    BioGridi34968. 16 interactions.
    DIPiDIP-4716N.
    IntActiP40545. 1 interaction.
    MINTiMINT-561085.
    STRINGi4932.YIL020C.

    Structurei

    Secondary structure

    1
    261
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 115
    Helixi41 – 5010
    Beta strandi57 – 648
    Helixi66 – 7510
    Turni77 – 793
    Beta strandi80 – 856
    Turni88 – 903
    Helixi91 – 944
    Turni95 – 973
    Beta strandi101 – 1033
    Helixi105 – 1073
    Helixi116 – 12611
    Helixi128 – 1303
    Beta strandi131 – 14111
    Beta strandi144 – 1496
    Turni150 – 1534
    Beta strandi154 – 1618
    Helixi162 – 1687
    Turni169 – 1713
    Beta strandi173 – 1786
    Helixi191 – 20111
    Beta strandi208 – 2136
    Helixi220 – 2289
    Beta strandi232 – 2354
    Helixi241 – 2433
    Beta strandi246 – 2483
    Helixi250 – 26011

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2AGKX-ray1.30A2-261[»]
    ProteinModelPortaliP40545.
    SMRiP40545. Positions 2-261.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP40545.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the HisA/HisF family.Curated

    Phylogenomic databases

    eggNOGiCOG0106.
    HOGENOMiHOG000172261.
    KOiK01814.
    OMAiFVAMNKW.
    OrthoDBiEOG7ZWDC9.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR006062. His_biosynth.
    IPR011858. HisA_euk.
    IPR011060. RibuloseP-bd_barrel.
    [Graphical view]
    PANTHERiPTHR21169. PTHR21169. 1 hit.
    PfamiPF00977. His_biosynth. 1 hit.
    [Graphical view]
    SUPFAMiSSF51366. SSF51366. 1 hit.
    TIGRFAMsiTIGR02129. hisA_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P40545-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKFIGCIDL HNGEVKQIVG GTLTSKKEDV PKTNFVSQHP SSYYAKLYKD    50
    RDVQGCHVIK LGPNNDDAAR EALQESPQFL QVGGGINDTN CLEWLKWASK 100
    VIVTSWLFTK EGHFQLKRLE RLTELCGKDR IVVDLSCRKT QDGRWIVAMN 150
    KWQTLTDLEL NADTFRELRK YTNEFLIHAA DVEGLCGGID ELLVSKLFEW 200
    TKDYDDLKIV YAGGAKSVDD LKLVDELSHG KVDLTFGSSL DIFGGNLVKF 250
    EDCCRWNEKQ G 261
    Length:261
    Mass (Da):29,557
    Last modified:February 1, 1995 - v1
    Checksum:iFA33F3A8ED52B526
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti243 – 2431F → S in AAS56185. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87341 Genomic DNA. Translation: CAA60779.1.
    Z46881 Genomic DNA. Translation: CAA86972.1.
    AY557859 Genomic DNA. Translation: AAS56185.1.
    BK006942 Genomic DNA. Translation: DAA08525.1.
    PIRiS49962.
    RefSeqiNP_012244.3. NM_001179370.3.

    Genome annotation databases

    EnsemblFungiiYIL020C; YIL020C; YIL020C.
    GeneIDi854792.
    KEGGisce:YIL020C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87341 Genomic DNA. Translation: CAA60779.1 .
    Z46881 Genomic DNA. Translation: CAA86972.1 .
    AY557859 Genomic DNA. Translation: AAS56185.1 .
    BK006942 Genomic DNA. Translation: DAA08525.1 .
    PIRi S49962.
    RefSeqi NP_012244.3. NM_001179370.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2AGK X-ray 1.30 A 2-261 [» ]
    ProteinModelPortali P40545.
    SMRi P40545. Positions 2-261.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34968. 16 interactions.
    DIPi DIP-4716N.
    IntActi P40545. 1 interaction.
    MINTi MINT-561085.
    STRINGi 4932.YIL020C.

    Proteomic databases

    MaxQBi P40545.
    PaxDbi P40545.
    PeptideAtlasi P40545.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL020C ; YIL020C ; YIL020C .
    GeneIDi 854792.
    KEGGi sce:YIL020C.

    Organism-specific databases

    CYGDi YIL020c.
    SGDi S000001282. HIS6.

    Phylogenomic databases

    eggNOGi COG0106.
    HOGENOMi HOG000172261.
    KOi K01814.
    OMAi FVAMNKW.
    OrthoDBi EOG7ZWDC9.

    Enzyme and pathway databases

    UniPathwayi UPA00031 ; UER00009 .
    BioCyci YEAST:YIL020C-MONOMER.
    BRENDAi 5.3.1.16. 984.

    Miscellaneous databases

    EvolutionaryTracei P40545.
    NextBioi 977588.

    Gene expression databases

    Genevestigatori P40545.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR006062. His_biosynth.
    IPR011858. HisA_euk.
    IPR011060. RibuloseP-bd_barrel.
    [Graphical view ]
    PANTHERi PTHR21169. PTHR21169. 1 hit.
    Pfami PF00977. His_biosynth. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51366. SSF51366. 1 hit.
    TIGRFAMsi TIGR02129. hisA_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Paralogous histidine biosynthetic genes: evolutionary analysis of the Saccharomyces cerevisiae HIS6 and HIS7 genes."
      Fani R., Tamburini E., Mori E., Lazcano A., Lio P., Barberio C., Casalone E., Cavalieri D., Perito B., Polsinelli M.
      Gene 197:9-17(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: YNN 282.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Crystal structure of the yeast His6 enzyme suggests a reaction mechanism."
      Quevillon-Cheruel S., Leulliot N., Graille M., Blondeau K., Janin J., van Tilbeurgh H.
      Protein Sci. 15:1516-1521(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS) OF 2-261.

    Entry informationi

    Entry nameiHIS4_YEAST
    AccessioniPrimary (citable) accession number: P40545
    Secondary accession number(s): D6VVQ9, Q6Q5P5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 6490 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3