ID   YKE4_YEAST              Reviewed;         346 AA.
AC   P40544; D6VVQ6;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   27-MAR-2024, entry version 162.
DE   RecName: Full=Zinc transporter YKE4;
GN   Name=YKE4; OrderedLocusNames=YIL023C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169870;
RA   Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA   Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA   Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA   Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA   Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL   Nature 387:84-87(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=16760462; DOI=10.1074/jbc.m604730200;
RA   Kumanovics A., Poruk K.E., Osborn K.A., Ward D.M., Kaplan J.;
RT   "YKE4 (YIL023C) encodes a bidirectional zinc transporter in the endoplasmic
RT   reticulum of Saccharomyces cerevisiae.";
RL   J. Biol. Chem. 281:22566-22574(2006).
RN   [4]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 208353 / W303-1A;
RX   PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA   Kim H., Melen K., Oesterberg M., von Heijne G.;
RT   "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC   -!- FUNCTION: Zinc transporter whose role depends on the zinc status of the
CC       cells. It helps to balance zinc levels between the cytosol and the
CC       secretory pathway. It transports zinc into the secretory pathway in a
CC       zinc-adequate environment and in a high zinc medium. In high zinc
CC       medium, transport of zinc into the secretory pathway is a way to
CC       eliminate zinc from the cytosol. Under low cytosolic zinc conditions,
CC       it removes zinc from the secretory pathway and acts as a zinc importer
CC       that helps to alleviate ER stress. {ECO:0000269|PubMed:16760462}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000269|PubMed:16760462}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:16760462}.
CC   -!- SIMILARITY: Belongs to the ZIP transporter (TC 2.A.5) family.
CC       KE4/Catsup subfamily. {ECO:0000305}.
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DR   EMBL; Z46881; CAA86969.1; -; Genomic_DNA.
DR   EMBL; BK006942; DAA08522.1; -; Genomic_DNA.
DR   PIR; S49959; S49959.
DR   RefSeq; NP_012241.1; NM_001179373.1.
DR   AlphaFoldDB; P40544; -.
DR   SMR; P40544; -.
DR   BioGRID; 34965; 56.
DR   DIP; DIP-5638N; -.
DR   STRING; 4932.YIL023C; -.
DR   TCDB; 2.A.5.4.4; the zinc (zn(2+))-iron (fe(2+)) permease (zip) family.
DR   GlyCosmos; P40544; 3 sites, No reported glycans.
DR   GlyGen; P40544; 3 sites.
DR   PaxDb; 4932-YIL023C; -.
DR   PeptideAtlas; P40544; -.
DR   EnsemblFungi; YIL023C_mRNA; YIL023C; YIL023C.
DR   GeneID; 854789; -.
DR   KEGG; sce:YIL023C; -.
DR   AGR; SGD:S000001285; -.
DR   SGD; S000001285; YKE4.
DR   VEuPathDB; FungiDB:YIL023C; -.
DR   eggNOG; KOG2693; Eukaryota.
DR   GeneTree; ENSGT00940000157349; -.
DR   HOGENOM; CLU_015114_0_0_1; -.
DR   InParanoid; P40544; -.
DR   OMA; TGDSVHC; -.
DR   OrthoDB; 5488029at2759; -.
DR   BioCyc; YEAST:G3O-31298-MONOMER; -.
DR   Reactome; R-SCE-442380; Zinc influx into cells by the SLC39 gene family.
DR   BioGRID-ORCS; 854789; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P40544; -.
DR   Proteomes; UP000002311; Chromosome IX.
DR   RNAct; P40544; Protein.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:SGD.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005385; F:zinc ion transmembrane transporter activity; IMP:SGD.
DR   GO; GO:0006882; P:intracellular zinc ion homeostasis; IBA:GO_Central.
DR   GO; GO:0071577; P:zinc ion transmembrane transport; IBA:GO_Central.
DR   GO; GO:0006829; P:zinc ion transport; IMP:SGD.
DR   InterPro; IPR003689; ZIP.
DR   PANTHER; PTHR16950; ZINC TRANSPORTER SLC39A7 HISTIDINE-RICH MEMBRANE PROTEIN KE4; 1.
DR   PANTHER; PTHR16950:SF16; ZINC TRANSPORTER ZIP13; 1.
DR   Pfam; PF02535; Zip; 1.
PE   1: Evidence at protein level;
KW   Endoplasmic reticulum; Glycoprotein; Ion transport; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport; Zinc;
KW   Zinc transport.
FT   CHAIN           1..346
FT                   /note="Zinc transporter YKE4"
FT                   /id="PRO_0000213698"
FT   TOPO_DOM        1..2
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        24..69
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        70..90
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        91..99
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        121..126
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        127..147
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        148..202
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        203..223
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        224..252
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        253..273
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        274..290
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        291..311
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        312..322
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        323..343
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        344..346
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        184
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        274
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        285
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   346 AA;  37418 MW;  645960E194778006 CRC64;
     MKASHICSYL LSIAPLVVSH GVHHNRDHGH EANHESKQSF LILKQESIFY SLVCFLQNHL
     FVLGPRYNAI VAILIIQLMP CLFVLFVPGL RKNDRASLTL SLLVSFSLGT LLGDILLHVI
     PESLSGVTDV TMVGGAIFLG FISFLTLDKT MRILSGTSND DGSIHSHSHS HTPQQTAEKK
     AGFNMSAYLN VISGIAHHIT DGIALATSFY SSTQVGIMTS IAVTFHEIPH ELGDFAILLS
     SGFTFPQAIR AQAVTAFGAV VGTSIGCWMN EIGNNSHKAT SSSANASELM LPFTAGGLIY
     IATTSVVPQI LHSSAPDSKL REFKKWALQL VFIFVGFAVM ALMDEH
//