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P40544 (YKE4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Zinc transporter YKE4
Gene names
Name:YKE4
Ordered Locus Names:YIL023C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Zinc transporter whose role depends on the zinc status of the cells. It helps to balance zinc levels between the cytosol and the secretory pathway. It transports zinc into the secretory pathway in a zinc-adequate environment and in a high zinc medium. In high zinc medium, transport of zinc into the secretory pathway is a way to eliminate zinc from the cytosol. Under low cytosolic zinc conditions, it removes zinc from the secretory pathway and acts as zinc importer that helps to alleviate ER stress. Ref.3

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.3.

Sequence similarities

Belongs to the ZIP transporter (TC 2.A.5) family. KE4/Catsup subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 346346Zinc transporter YKE4
PRO_0000213698

Regions

Topological domain1 – 22Extracellular Potential
Transmembrane3 – 2321Helical; Potential
Topological domain24 – 6946Cytoplasmic Potential
Transmembrane70 – 9021Helical; Potential
Topological domain91 – 999Extracellular Potential
Transmembrane100 – 12021Helical; Potential
Topological domain121 – 1266Cytoplasmic Potential
Transmembrane127 – 14721Helical; Potential
Topological domain148 – 20255Extracellular Potential
Transmembrane203 – 22321Helical; Potential
Topological domain224 – 25229Cytoplasmic Potential
Transmembrane253 – 27321Helical; Potential
Topological domain274 – 29017Extracellular Potential
Transmembrane291 – 31121Helical; Potential
Topological domain312 – 32211Cytoplasmic Potential
Transmembrane323 – 34321Helical; Potential
Topological domain344 – 3463Extracellular Potential

Amino acid modifications

Glycosylation1841N-linked (GlcNAc...) Potential
Glycosylation2741N-linked (GlcNAc...) Potential
Glycosylation2851N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P40544 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 645960E194778006

FASTA34637,418
        10         20         30         40         50         60 
MKASHICSYL LSIAPLVVSH GVHHNRDHGH EANHESKQSF LILKQESIFY SLVCFLQNHL 

        70         80         90        100        110        120 
FVLGPRYNAI VAILIIQLMP CLFVLFVPGL RKNDRASLTL SLLVSFSLGT LLGDILLHVI 

       130        140        150        160        170        180 
PESLSGVTDV TMVGGAIFLG FISFLTLDKT MRILSGTSND DGSIHSHSHS HTPQQTAEKK 

       190        200        210        220        230        240 
AGFNMSAYLN VISGIAHHIT DGIALATSFY SSTQVGIMTS IAVTFHEIPH ELGDFAILLS 

       250        260        270        280        290        300 
SGFTFPQAIR AQAVTAFGAV VGTSIGCWMN EIGNNSHKAT SSSANASELM LPFTAGGLIY 

       310        320        330        340 
IATTSVVPQI LHSSAPDSKL REFKKWALQL VFIFVGFAVM ALMDEH

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"YKE4 (YIL023C) encodes a bidirectional zinc transporter in the endoplasmic reticulum of Saccharomyces cerevisiae."
Kumanovics A., Poruk K.E., Osborn K.A., Ward D.M., Kaplan J.
J. Biol. Chem. 281:22566-22574(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z46881 Genomic DNA. Translation: CAA86969.1.
BK006942 Genomic DNA. Translation: DAA08522.1.
PIRS49959.
RefSeqNP_012241.1. NM_001179373.1.

3D structure databases

ProteinModelPortalP40544.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-5638N.
MINTMINT-480644.
STRING4932.YIL023C.

Protein family/group databases

TCDB2.A.5.4.4. zinc (Zn2+)-iron (Fe2+) permease (ZIP) family.

Proteomic databases

PaxDbP40544.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIL023C; YIL023C; YIL023C.
GeneID854789.
KEGGsce:YIL023C.

Organism-specific databases

CYGDYIL023c.
SGDS000001285. YKE4.

Phylogenomic databases

eggNOGCOG0428.
GeneTreeENSGT00700000104299.
HOGENOMHOG000185308.
KOK14713.
OMAFAHEIPH.
OrthoDBEOG422DV8.

Gene expression databases

GenevestigatorP40544.
GermOnlineYIL023C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR003689. ZIP.
[Graphical view]
PfamPF02535. Zip. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977579.

Entry information

Entry nameYKE4_YEAST
AccessionPrimary (citable) accession number: P40544
Secondary accession number(s): D6VVQ6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 3, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

SIMILARITY comments

Index of protein domains and families

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