Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P40541

- SCC3_YEAST

UniProt

P40541 - SCC3_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Cohesin subunit SCC3

Gene

IRR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the MCD1/SCC1 subunit of the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.

GO - Molecular functioni

  1. chromatin binding Source: SGD

GO - Biological processi

  1. cytogamy Source: SGD
  2. fungal-type cell wall organization Source: SGD
  3. mitotic sister chromatid cohesion Source: SGD
  4. protein acetylation Source: UniProtKB
  5. spore germination Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-31300-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cohesin subunit SCC3
Alternative name(s):
Irregular cell behavior protein 1
Gene namesi
Name:IRR1
Synonyms:SCC3
Ordered Locus Names:YIL026C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIL026c.
SGDiS000001288. IRR1.

Subcellular locationi

Nucleus 1 PublicationPROSITE-ProRule annotation. Chromosome 1 Publication. Chromosomecentromere 1 Publication
Note: Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin except at centromeres, where cohesin complexes remain. At anaphase, the MCD1 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.

GO - Cellular componenti

  1. chromosome, centromeric region Source: UniProtKB-KW
  2. cytosol Source: SGD
  3. nuclear mitotic cohesin complex Source: SGD
  4. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11501150Cohesin subunit SCC3PRO_0000120191Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei28 – 281Phosphoserine1 Publication
Modified residuei628 – 6281Phosphoserine1 Publication

Post-translational modificationi

Acetylated by ECO1.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP40541.
PaxDbiP40541.
PeptideAtlasiP40541.

Expressioni

Gene expression databases

GenevestigatoriP40541.

Interactioni

Subunit structurei

Interacts directly with MCD1 in cohesin complex. Cohesin complexes are composed of the SMC1 and SMC3 heterodimer attached via their hinge domain, MCD1 which link them, and IRR1/SCC3, which interacts with MCD1. The cohesin complex also interacts with SCC2, which is required for its association with chromosomes. Interacts with LIN1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MCD1Q121585EBI-16667,EBI-16655
SMC1P329082EBI-16667,EBI-17402
SMC3P470372EBI-16667,EBI-17423

Protein-protein interaction databases

BioGridi34963. 29 interactions.
DIPiDIP-5640N.
IntActiP40541. 20 interactions.
MINTiMINT-567741.
STRINGi4932.YIL026C.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4UVJX-ray2.10A/B674-1072[»]
ProteinModelPortaliP40541.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini367 – 45791SCDPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili305 – 34945Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi44 – 485Poly-Glu
Compositional biasi65 – 706Poly-Asp

Sequence similaritiesi

Belongs to the SCC3 family.Curated
Contains 1 SCD (stromalin conservative) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5537.
GeneTreeiENSGT00390000014094.
HOGENOMiHOG000141696.
InParanoidiP40541.
KOiK06671.
OMAiKYFGWLL.
OrthoDBiEOG7QRR35.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR020839. SCD.
IPR013721. STAG.
[Graphical view]
PfamiPF08514. STAG. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS51425. SCD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40541-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTAVRRSTRI RTKSQVIEED YDDEQNTSAQ HVESDKITAK TQHEEEEEQD
60 70 80 90 100
TGESEESSSE DDYEDQDDDD YVDTATAKRK SRKRKPKSAS NTSSKRQKKK
110 120 130 140 150
PTSAQKSAVS HAPAYHRSKK DQDQYLEIAK DFQPTELFDI LSTSEDVSIE
160 170 180 190 200
ELLREWLETY SENRDKFLQE FINLLLNCCG SVARVEDHDV HSNESSNETI
210 220 230 240 250
GEIQLLFQRQ KLHEFYLLIS KENKKRKNFK MGPLYQNFAE FMTKLLEVAN
260 270 280 290 300
DLQLLYVESD EDDTQIVTGN LVLDLLTWLS SFSVCKIRCF RYISTLTLYL
310 320 330 340 350
FQDYLTQQAV NLEKNYLAKL SKQLSLEEKK KRPNNKTLEK LESTIAETQG
360 370 380 390 400
SKVVIDSIID NIVKLCFVHR YKDVSDLIRS ESMLHLSIWI KNYPEYFLKV
410 420 430 440 450
TFLKYFGWLL SDNSVSVRLQ VTKILPHLII QNHNSKSTDN SAIRQVFERF
460 470 480 490 500
KTKILEVAIR DVNLDVRIHS IQVLTEASSL GYLDDSEILI ISSLMFDEEF
510 520 530 540 550
DPFKTSSFNK RSKFLSTVAK FLARVIKEKF DEFIKTHEDL PKEVDGLEVG
560 570 580 590 600
PVVQVGIFIK ILNDSLIYHL KDCAEVDSRT KIRMLTQAAE FLSPYISTHL
610 620 630 640 650
KTICNLLISD TESNELIQKL QNSANNNSDD EDVDDEELDI TPLFPIDRNS
660 670 680 690 700
TILYLNVFHG LCAGANNPKI QTKDSVKEIV LPLFYDLLNA ASIESADILC
710 720 730 740 750
PLLESFITFS LDDWISIGYE TELKKITDKT IKAFMDSTIG NSKVDMKYDI
760 770 780 790 800
FAKFIHHIHH FEKKELQEKF LNQIATLKIH LKKFLQEKMD PNNSRDDYKD
810 820 830 840 850
LTCSLYELYI NKLTILGRDY PIEVDEELLQ LFLNNFVSRI PIMFQDFDDS
860 870 880 890 900
TAQEINFKML VLLATWNLEK WREIIEKVRD YENSISKDLR SVWKPIAAII
910 920 930 940 950
GRLNTLVISL AATNETFENI NSLFYLKWSA CTSLMDIIVA IKIFELKLPA
960 970 980 990 1000
DATTWRYSMS EQFPFYLHDN ASKVLLKIFL YLESLFAKQV DVQLERVADE
1010 1020 1030 1040 1050
DANLNDLPET GFFENIETEF LLFTVKLKGL MKLNILDERF ASRVALNKEK
1060 1070 1080 1090 1100
LGPLFKKIVD DTIMENPEPN KKNIQKAKSN QTQREKAPLQ PNSERETDHA
1110 1120 1130 1140 1150
NTENNDPDIP MTIDLEPIEE SSQNNSELAP IEEHPTVVDA IDNSDEITQD
Length:1,150
Mass (Da):133,009
Last modified:February 1, 1995 - v1
Checksum:i89688EA09485AC28
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti939 – 9391V → G in AAC49039. (PubMed:7483852)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U17918 Genomic DNA. Translation: AAC49039.1.
Z46881 Genomic DNA. Translation: CAA86966.1.
BK006942 Genomic DNA. Translation: DAA08520.1.
PIRiS49956.
RefSeqiNP_012238.1. NM_001179376.1.

Genome annotation databases

EnsemblFungiiYIL026C; YIL026C; YIL026C.
GeneIDi854786.
KEGGisce:YIL026C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U17918 Genomic DNA. Translation: AAC49039.1 .
Z46881 Genomic DNA. Translation: CAA86966.1 .
BK006942 Genomic DNA. Translation: DAA08520.1 .
PIRi S49956.
RefSeqi NP_012238.1. NM_001179376.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4UVJ X-ray 2.10 A/B 674-1072 [» ]
ProteinModelPortali P40541.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34963. 29 interactions.
DIPi DIP-5640N.
IntActi P40541. 20 interactions.
MINTi MINT-567741.
STRINGi 4932.YIL026C.

Proteomic databases

MaxQBi P40541.
PaxDbi P40541.
PeptideAtlasi P40541.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIL026C ; YIL026C ; YIL026C .
GeneIDi 854786.
KEGGi sce:YIL026C.

Organism-specific databases

CYGDi YIL026c.
SGDi S000001288. IRR1.

Phylogenomic databases

eggNOGi COG5537.
GeneTreei ENSGT00390000014094.
HOGENOMi HOG000141696.
InParanoidi P40541.
KOi K06671.
OMAi KYFGWLL.
OrthoDBi EOG7QRR35.

Enzyme and pathway databases

BioCyci YEAST:G3O-31300-MONOMER.

Miscellaneous databases

NextBioi 977573.

Gene expression databases

Genevestigatori P40541.

Family and domain databases

InterProi IPR016024. ARM-type_fold.
IPR020839. SCD.
IPR013721. STAG.
[Graphical view ]
Pfami PF08514. STAG. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 2 hits.
PROSITEi PS51425. SCD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A new essential gene located on Saccharomyces cerevisiae chromosome IX."
    Kurlandzka A., Rytka J., Gromadka R., Murawski M.
    Yeast 11:885-890(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication."
    Toth A., Ciosk R., Uhlmann F., Galova M., Schleiffer A., Nasmyth K.
    Genes Dev. 13:320-333(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH MCD1, IDENTIFICATION IN A COHESIN COMPLEX WITH SMC1; SMC3 AND MCD1, INTERACTION OF THE COHESIN COMPLEX WITH SCC2.
  5. "Proteins interacting with Lin 1p, a putative link between chromosome segregation, mRNA splicing and DNA replication in Saccharomyces cerevisiae."
    Bialkowska A., Kurlandzka A.
    Yeast 19:1323-1333(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LIN1.
  6. "Molecular architecture of SMC proteins and the yeast cohesin complex."
    Haering C.H., Loewe J., Hochwagen A., Nasmyth K.
    Mol. Cell 9:773-788(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COHESIN COMPLEX WITH SMC1; SMC3 AND MCD1, STRUCTURE.
  7. "Eco1 is a novel acetyltransferase that can acetylate proteins involved in cohesion."
    Ivanov D., Schleiffer A., Eisenhaber F., Mechtler K., Haering C.H., Nasmyth K.
    Curr. Biol. 12:323-328(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28 AND SER-628, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSCC3_YEAST
AccessioniPrimary (citable) accession number: P40541
Secondary accession number(s): D6VVQ4, Q02511
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4090 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3