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Protein

Cohesin subunit SCC3

Gene

IRR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the MCD1/SCC1 subunit of the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.

GO - Molecular functioni

  • chromatin binding Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic sister chromatid cohesion Source: SGD
  • protein acetylation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-31300-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cohesin subunit SCC3
Alternative name(s):
Irregular cell behavior protein 1
Gene namesi
Name:IRR1
Synonyms:SCC3
Ordered Locus Names:YIL026C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL026C.
SGDiS000001288. IRR1.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication
  • Chromosome 1 Publication
  • Chromosomecentromere 1 Publication

  • Note: Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin except at centromeres, where cohesin complexes remain. At anaphase, the MCD1 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.

GO - Cellular componenti

  • chromosome, centromeric region Source: UniProtKB-SubCell
  • cytosol Source: SGD
  • nuclear mitotic cohesin complex Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001201911 – 1150Cohesin subunit SCC3Add BLAST1150

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei28PhosphoserineCombined sources1
Modified residuei628PhosphoserineCombined sources1

Post-translational modificationi

Acetylated by ECO1.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP40541.
PRIDEiP40541.

PTM databases

iPTMnetiP40541.

Interactioni

Subunit structurei

Interacts directly with MCD1 in cohesin complex. Cohesin complexes are composed of the SMC1 and SMC3 heterodimer attached via their hinge domain, MCD1 which link them, and IRR1/SCC3, which interacts with MCD1. The cohesin complex also interacts with SCC2, which is required for its association with chromosomes. Interacts with LIN1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MCD1Q121585EBI-16667,EBI-16655
SMC1P329082EBI-16667,EBI-17402
SMC3P470372EBI-16667,EBI-17423

Protein-protein interaction databases

BioGridi34963. 29 interactors.
DIPiDIP-5640N.
IntActiP40541. 20 interactors.
MINTiMINT-567741.

Structurei

Secondary structure

11150
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi675 – 678Combined sources4
Helixi680 – 691Combined sources12
Helixi700 – 707Combined sources8
Helixi721 – 736Combined sources16
Turni742 – 744Combined sources3
Helixi747 – 761Combined sources15
Helixi764 – 788Combined sources25
Helixi798 – 808Combined sources11
Helixi810 – 819Combined sources10
Helixi826 – 835Combined sources10
Helixi837 – 839Combined sources3
Helixi840 – 843Combined sources4
Helixi844 – 846Combined sources3
Helixi849 – 854Combined sources6
Helixi858 – 876Combined sources19
Turni890 – 893Combined sources4
Helixi894 – 911Combined sources18
Turni917 – 919Combined sources3
Helixi920 – 946Combined sources27
Helixi953 – 962Combined sources10
Helixi969 – 989Combined sources21
Helixi1002 – 1005Combined sources4
Helixi1016 – 1032Combined sources17
Helixi1038 – 1045Combined sources8
Turni1046 – 1050Combined sources5
Helixi1053 – 1058Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4UVJX-ray2.10A/B674-1072[»]
ProteinModelPortaliP40541.
SMRiP40541.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini367 – 457SCDPROSITE-ProRule annotationAdd BLAST91

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili305 – 349Sequence analysisAdd BLAST45

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi44 – 48Poly-Glu5
Compositional biasi65 – 70Poly-Asp6

Sequence similaritiesi

Belongs to the SCC3 family.Curated
Contains 1 SCD (stromalin conservative) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000014094.
HOGENOMiHOG000141696.
InParanoidiP40541.
KOiK06671.
OMAiKYFGWLL.
OrthoDBiEOG092C0RMX.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR020839. SCD.
IPR013721. STAG.
[Graphical view]
PfamiPF08514. STAG. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS51425. SCD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40541-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAVRRSTRI RTKSQVIEED YDDEQNTSAQ HVESDKITAK TQHEEEEEQD
60 70 80 90 100
TGESEESSSE DDYEDQDDDD YVDTATAKRK SRKRKPKSAS NTSSKRQKKK
110 120 130 140 150
PTSAQKSAVS HAPAYHRSKK DQDQYLEIAK DFQPTELFDI LSTSEDVSIE
160 170 180 190 200
ELLREWLETY SENRDKFLQE FINLLLNCCG SVARVEDHDV HSNESSNETI
210 220 230 240 250
GEIQLLFQRQ KLHEFYLLIS KENKKRKNFK MGPLYQNFAE FMTKLLEVAN
260 270 280 290 300
DLQLLYVESD EDDTQIVTGN LVLDLLTWLS SFSVCKIRCF RYISTLTLYL
310 320 330 340 350
FQDYLTQQAV NLEKNYLAKL SKQLSLEEKK KRPNNKTLEK LESTIAETQG
360 370 380 390 400
SKVVIDSIID NIVKLCFVHR YKDVSDLIRS ESMLHLSIWI KNYPEYFLKV
410 420 430 440 450
TFLKYFGWLL SDNSVSVRLQ VTKILPHLII QNHNSKSTDN SAIRQVFERF
460 470 480 490 500
KTKILEVAIR DVNLDVRIHS IQVLTEASSL GYLDDSEILI ISSLMFDEEF
510 520 530 540 550
DPFKTSSFNK RSKFLSTVAK FLARVIKEKF DEFIKTHEDL PKEVDGLEVG
560 570 580 590 600
PVVQVGIFIK ILNDSLIYHL KDCAEVDSRT KIRMLTQAAE FLSPYISTHL
610 620 630 640 650
KTICNLLISD TESNELIQKL QNSANNNSDD EDVDDEELDI TPLFPIDRNS
660 670 680 690 700
TILYLNVFHG LCAGANNPKI QTKDSVKEIV LPLFYDLLNA ASIESADILC
710 720 730 740 750
PLLESFITFS LDDWISIGYE TELKKITDKT IKAFMDSTIG NSKVDMKYDI
760 770 780 790 800
FAKFIHHIHH FEKKELQEKF LNQIATLKIH LKKFLQEKMD PNNSRDDYKD
810 820 830 840 850
LTCSLYELYI NKLTILGRDY PIEVDEELLQ LFLNNFVSRI PIMFQDFDDS
860 870 880 890 900
TAQEINFKML VLLATWNLEK WREIIEKVRD YENSISKDLR SVWKPIAAII
910 920 930 940 950
GRLNTLVISL AATNETFENI NSLFYLKWSA CTSLMDIIVA IKIFELKLPA
960 970 980 990 1000
DATTWRYSMS EQFPFYLHDN ASKVLLKIFL YLESLFAKQV DVQLERVADE
1010 1020 1030 1040 1050
DANLNDLPET GFFENIETEF LLFTVKLKGL MKLNILDERF ASRVALNKEK
1060 1070 1080 1090 1100
LGPLFKKIVD DTIMENPEPN KKNIQKAKSN QTQREKAPLQ PNSERETDHA
1110 1120 1130 1140 1150
NTENNDPDIP MTIDLEPIEE SSQNNSELAP IEEHPTVVDA IDNSDEITQD
Length:1,150
Mass (Da):133,009
Last modified:February 1, 1995 - v1
Checksum:i89688EA09485AC28
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti939V → G in AAC49039 (PubMed:7483852).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17918 Genomic DNA. Translation: AAC49039.1.
Z46881 Genomic DNA. Translation: CAA86966.1.
BK006942 Genomic DNA. Translation: DAA08520.1.
PIRiS49956.
RefSeqiNP_012238.1. NM_001179376.1.

Genome annotation databases

EnsemblFungiiYIL026C; YIL026C; YIL026C.
GeneIDi854786.
KEGGisce:YIL026C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17918 Genomic DNA. Translation: AAC49039.1.
Z46881 Genomic DNA. Translation: CAA86966.1.
BK006942 Genomic DNA. Translation: DAA08520.1.
PIRiS49956.
RefSeqiNP_012238.1. NM_001179376.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4UVJX-ray2.10A/B674-1072[»]
ProteinModelPortaliP40541.
SMRiP40541.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34963. 29 interactors.
DIPiDIP-5640N.
IntActiP40541. 20 interactors.
MINTiMINT-567741.

PTM databases

iPTMnetiP40541.

Proteomic databases

MaxQBiP40541.
PRIDEiP40541.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL026C; YIL026C; YIL026C.
GeneIDi854786.
KEGGisce:YIL026C.

Organism-specific databases

EuPathDBiFungiDB:YIL026C.
SGDiS000001288. IRR1.

Phylogenomic databases

GeneTreeiENSGT00390000014094.
HOGENOMiHOG000141696.
InParanoidiP40541.
KOiK06671.
OMAiKYFGWLL.
OrthoDBiEOG092C0RMX.

Enzyme and pathway databases

BioCyciYEAST:G3O-31300-MONOMER.

Miscellaneous databases

PROiP40541.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR020839. SCD.
IPR013721. STAG.
[Graphical view]
PfamiPF08514. STAG. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS51425. SCD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSCC3_YEAST
AccessioniPrimary (citable) accession number: P40541
Secondary accession number(s): D6VVQ4, Q02511
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4090 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.