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P40541

- SCC3_YEAST

UniProt

P40541 - SCC3_YEAST

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Protein
Cohesin subunit SCC3
Gene
IRR1, SCC3, YIL026C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the MCD1/SCC1 subunit of the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.

GO - Molecular functioni

  1. chromatin binding Source: SGD
  2. protein binding Source: IntAct

GO - Biological processi

  1. cytogamy Source: SGD
  2. fungal-type cell wall organization Source: SGD
  3. mitotic sister chromatid cohesion Source: SGD
  4. protein acetylation Source: UniProtKB
  5. spore germination Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-31300-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cohesin subunit SCC3
Alternative name(s):
Irregular cell behavior protein 1
Gene namesi
Name:IRR1
Synonyms:SCC3
Ordered Locus Names:YIL026C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIL026c.
SGDiS000001288. IRR1.

Subcellular locationi

Nucleus. Chromosome. Chromosomecentromere
Note: Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin except at centromeres, where cohesin complexes remain. At anaphase, the MCD1 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.1 Publication

GO - Cellular componenti

  1. chromosome, centromeric region Source: UniProtKB-SubCell
  2. cytosol Source: SGD
  3. nuclear mitotic cohesin complex Source: SGD
  4. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11501150Cohesin subunit SCC3
PRO_0000120191Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei28 – 281Phosphoserine1 Publication
Modified residuei628 – 6281Phosphoserine1 Publication

Post-translational modificationi

Acetylated by ECO1.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP40541.
PaxDbiP40541.
PeptideAtlasiP40541.

Expressioni

Gene expression databases

GenevestigatoriP40541.

Interactioni

Subunit structurei

Interacts directly with MCD1 in cohesin complex. Cohesin complexes are composed of the SMC1 and SMC3 heterodimer attached via their hinge domain, MCD1 which link them, and IRR1/SCC3, which interacts with MCD1. The cohesin complex also interacts with SCC2, which is required for its association with chromosomes. Interacts with LIN1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MCD1Q121585EBI-16667,EBI-16655
SMC1P329082EBI-16667,EBI-17402
SMC3P470372EBI-16667,EBI-17423

Protein-protein interaction databases

BioGridi34963. 28 interactions.
DIPiDIP-5640N.
IntActiP40541. 20 interactions.
MINTiMINT-567741.
STRINGi4932.YIL026C.

Structurei

3D structure databases

ProteinModelPortaliP40541.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini367 – 45791SCD
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili305 – 34945 Reviewed prediction
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi44 – 485Poly-Glu
Compositional biasi65 – 706Poly-Asp

Sequence similaritiesi

Belongs to the SCC3 family.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5537.
GeneTreeiENSGT00390000014094.
HOGENOMiHOG000141696.
KOiK06671.
OMAiKYFGWLL.
OrthoDBiEOG7QRR35.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR020839. SCD.
IPR013721. STAG.
[Graphical view]
PfamiPF08514. STAG. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS51425. SCD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40541-1 [UniParc]FASTAAdd to Basket

« Hide

MTAVRRSTRI RTKSQVIEED YDDEQNTSAQ HVESDKITAK TQHEEEEEQD     50
TGESEESSSE DDYEDQDDDD YVDTATAKRK SRKRKPKSAS NTSSKRQKKK 100
PTSAQKSAVS HAPAYHRSKK DQDQYLEIAK DFQPTELFDI LSTSEDVSIE 150
ELLREWLETY SENRDKFLQE FINLLLNCCG SVARVEDHDV HSNESSNETI 200
GEIQLLFQRQ KLHEFYLLIS KENKKRKNFK MGPLYQNFAE FMTKLLEVAN 250
DLQLLYVESD EDDTQIVTGN LVLDLLTWLS SFSVCKIRCF RYISTLTLYL 300
FQDYLTQQAV NLEKNYLAKL SKQLSLEEKK KRPNNKTLEK LESTIAETQG 350
SKVVIDSIID NIVKLCFVHR YKDVSDLIRS ESMLHLSIWI KNYPEYFLKV 400
TFLKYFGWLL SDNSVSVRLQ VTKILPHLII QNHNSKSTDN SAIRQVFERF 450
KTKILEVAIR DVNLDVRIHS IQVLTEASSL GYLDDSEILI ISSLMFDEEF 500
DPFKTSSFNK RSKFLSTVAK FLARVIKEKF DEFIKTHEDL PKEVDGLEVG 550
PVVQVGIFIK ILNDSLIYHL KDCAEVDSRT KIRMLTQAAE FLSPYISTHL 600
KTICNLLISD TESNELIQKL QNSANNNSDD EDVDDEELDI TPLFPIDRNS 650
TILYLNVFHG LCAGANNPKI QTKDSVKEIV LPLFYDLLNA ASIESADILC 700
PLLESFITFS LDDWISIGYE TELKKITDKT IKAFMDSTIG NSKVDMKYDI 750
FAKFIHHIHH FEKKELQEKF LNQIATLKIH LKKFLQEKMD PNNSRDDYKD 800
LTCSLYELYI NKLTILGRDY PIEVDEELLQ LFLNNFVSRI PIMFQDFDDS 850
TAQEINFKML VLLATWNLEK WREIIEKVRD YENSISKDLR SVWKPIAAII 900
GRLNTLVISL AATNETFENI NSLFYLKWSA CTSLMDIIVA IKIFELKLPA 950
DATTWRYSMS EQFPFYLHDN ASKVLLKIFL YLESLFAKQV DVQLERVADE 1000
DANLNDLPET GFFENIETEF LLFTVKLKGL MKLNILDERF ASRVALNKEK 1050
LGPLFKKIVD DTIMENPEPN KKNIQKAKSN QTQREKAPLQ PNSERETDHA 1100
NTENNDPDIP MTIDLEPIEE SSQNNSELAP IEEHPTVVDA IDNSDEITQD 1150
Length:1,150
Mass (Da):133,009
Last modified:February 1, 1995 - v1
Checksum:i89688EA09485AC28
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti939 – 9391V → G in AAC49039. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U17918 Genomic DNA. Translation: AAC49039.1.
Z46881 Genomic DNA. Translation: CAA86966.1.
BK006942 Genomic DNA. Translation: DAA08520.1.
PIRiS49956.
RefSeqiNP_012238.1. NM_001179376.1.

Genome annotation databases

EnsemblFungiiYIL026C; YIL026C; YIL026C.
GeneIDi854786.
KEGGisce:YIL026C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U17918 Genomic DNA. Translation: AAC49039.1 .
Z46881 Genomic DNA. Translation: CAA86966.1 .
BK006942 Genomic DNA. Translation: DAA08520.1 .
PIRi S49956.
RefSeqi NP_012238.1. NM_001179376.1.

3D structure databases

ProteinModelPortali P40541.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34963. 28 interactions.
DIPi DIP-5640N.
IntActi P40541. 20 interactions.
MINTi MINT-567741.
STRINGi 4932.YIL026C.

Proteomic databases

MaxQBi P40541.
PaxDbi P40541.
PeptideAtlasi P40541.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIL026C ; YIL026C ; YIL026C .
GeneIDi 854786.
KEGGi sce:YIL026C.

Organism-specific databases

CYGDi YIL026c.
SGDi S000001288. IRR1.

Phylogenomic databases

eggNOGi COG5537.
GeneTreei ENSGT00390000014094.
HOGENOMi HOG000141696.
KOi K06671.
OMAi KYFGWLL.
OrthoDBi EOG7QRR35.

Enzyme and pathway databases

BioCyci YEAST:G3O-31300-MONOMER.

Miscellaneous databases

NextBioi 977573.

Gene expression databases

Genevestigatori P40541.

Family and domain databases

InterProi IPR016024. ARM-type_fold.
IPR020839. SCD.
IPR013721. STAG.
[Graphical view ]
Pfami PF08514. STAG. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 2 hits.
PROSITEi PS51425. SCD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A new essential gene located on Saccharomyces cerevisiae chromosome IX."
    Kurlandzka A., Rytka J., Gromadka R., Murawski M.
    Yeast 11:885-890(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication."
    Toth A., Ciosk R., Uhlmann F., Galova M., Schleiffer A., Nasmyth K.
    Genes Dev. 13:320-333(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH MCD1, IDENTIFICATION IN A COHESIN COMPLEX WITH SMC1; SMC3 AND MCD1, INTERACTION OF THE COHESIN COMPLEX WITH SCC2.
  5. "Proteins interacting with Lin 1p, a putative link between chromosome segregation, mRNA splicing and DNA replication in Saccharomyces cerevisiae."
    Bialkowska A., Kurlandzka A.
    Yeast 19:1323-1333(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LIN1.
  6. "Molecular architecture of SMC proteins and the yeast cohesin complex."
    Haering C.H., Loewe J., Hochwagen A., Nasmyth K.
    Mol. Cell 9:773-788(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COHESIN COMPLEX WITH SMC1; SMC3 AND MCD1, STRUCTURE.
  7. "Eco1 is a novel acetyltransferase that can acetylate proteins involved in cohesion."
    Ivanov D., Schleiffer A., Eisenhaber F., Mechtler K., Haering C.H., Nasmyth K.
    Curr. Biol. 12:323-328(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28 AND SER-628, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSCC3_YEAST
AccessioniPrimary (citable) accession number: P40541
Secondary accession number(s): D6VVQ4, Q02511
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 9, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4090 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

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