Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P40541

- SCC3_YEAST

UniProt

P40541 - SCC3_YEAST

Protein

Cohesin subunit SCC3

Gene

IRR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the MCD1/SCC1 subunit of the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.

    GO - Molecular functioni

    1. chromatin binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. cytogamy Source: SGD
    2. fungal-type cell wall organization Source: SGD
    3. mitotic sister chromatid cohesion Source: SGD
    4. protein acetylation Source: UniProtKB
    5. spore germination Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Chromosome partition, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31300-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cohesin subunit SCC3
    Alternative name(s):
    Irregular cell behavior protein 1
    Gene namesi
    Name:IRR1
    Synonyms:SCC3
    Ordered Locus Names:YIL026C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL026c.
    SGDiS000001288. IRR1.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation. Chromosome 1 Publication. Chromosomecentromere 1 Publication
    Note: Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin except at centromeres, where cohesin complexes remain. At anaphase, the MCD1 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.

    GO - Cellular componenti

    1. chromosome, centromeric region Source: UniProtKB-SubCell
    2. cytosol Source: SGD
    3. nuclear mitotic cohesin complex Source: SGD
    4. nucleus Source: SGD

    Keywords - Cellular componenti

    Centromere, Chromosome, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11501150Cohesin subunit SCC3PRO_0000120191Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei28 – 281Phosphoserine1 Publication
    Modified residuei628 – 6281Phosphoserine1 Publication

    Post-translational modificationi

    Acetylated by ECO1.1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP40541.
    PaxDbiP40541.
    PeptideAtlasiP40541.

    Expressioni

    Gene expression databases

    GenevestigatoriP40541.

    Interactioni

    Subunit structurei

    Interacts directly with MCD1 in cohesin complex. Cohesin complexes are composed of the SMC1 and SMC3 heterodimer attached via their hinge domain, MCD1 which link them, and IRR1/SCC3, which interacts with MCD1. The cohesin complex also interacts with SCC2, which is required for its association with chromosomes. Interacts with LIN1.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MCD1Q121585EBI-16667,EBI-16655
    SMC1P329082EBI-16667,EBI-17402
    SMC3P470372EBI-16667,EBI-17423

    Protein-protein interaction databases

    BioGridi34963. 28 interactions.
    DIPiDIP-5640N.
    IntActiP40541. 20 interactions.
    MINTiMINT-567741.
    STRINGi4932.YIL026C.

    Structurei

    3D structure databases

    ProteinModelPortaliP40541.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini367 – 45791SCDPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili305 – 34945Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi44 – 485Poly-Glu
    Compositional biasi65 – 706Poly-Asp

    Sequence similaritiesi

    Belongs to the SCC3 family.Curated
    Contains 1 SCD (stromalin conservative) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5537.
    GeneTreeiENSGT00390000014094.
    HOGENOMiHOG000141696.
    KOiK06671.
    OMAiKYFGWLL.
    OrthoDBiEOG7QRR35.

    Family and domain databases

    InterProiIPR016024. ARM-type_fold.
    IPR020839. SCD.
    IPR013721. STAG.
    [Graphical view]
    PfamiPF08514. STAG. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 2 hits.
    PROSITEiPS51425. SCD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40541-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAVRRSTRI RTKSQVIEED YDDEQNTSAQ HVESDKITAK TQHEEEEEQD     50
    TGESEESSSE DDYEDQDDDD YVDTATAKRK SRKRKPKSAS NTSSKRQKKK 100
    PTSAQKSAVS HAPAYHRSKK DQDQYLEIAK DFQPTELFDI LSTSEDVSIE 150
    ELLREWLETY SENRDKFLQE FINLLLNCCG SVARVEDHDV HSNESSNETI 200
    GEIQLLFQRQ KLHEFYLLIS KENKKRKNFK MGPLYQNFAE FMTKLLEVAN 250
    DLQLLYVESD EDDTQIVTGN LVLDLLTWLS SFSVCKIRCF RYISTLTLYL 300
    FQDYLTQQAV NLEKNYLAKL SKQLSLEEKK KRPNNKTLEK LESTIAETQG 350
    SKVVIDSIID NIVKLCFVHR YKDVSDLIRS ESMLHLSIWI KNYPEYFLKV 400
    TFLKYFGWLL SDNSVSVRLQ VTKILPHLII QNHNSKSTDN SAIRQVFERF 450
    KTKILEVAIR DVNLDVRIHS IQVLTEASSL GYLDDSEILI ISSLMFDEEF 500
    DPFKTSSFNK RSKFLSTVAK FLARVIKEKF DEFIKTHEDL PKEVDGLEVG 550
    PVVQVGIFIK ILNDSLIYHL KDCAEVDSRT KIRMLTQAAE FLSPYISTHL 600
    KTICNLLISD TESNELIQKL QNSANNNSDD EDVDDEELDI TPLFPIDRNS 650
    TILYLNVFHG LCAGANNPKI QTKDSVKEIV LPLFYDLLNA ASIESADILC 700
    PLLESFITFS LDDWISIGYE TELKKITDKT IKAFMDSTIG NSKVDMKYDI 750
    FAKFIHHIHH FEKKELQEKF LNQIATLKIH LKKFLQEKMD PNNSRDDYKD 800
    LTCSLYELYI NKLTILGRDY PIEVDEELLQ LFLNNFVSRI PIMFQDFDDS 850
    TAQEINFKML VLLATWNLEK WREIIEKVRD YENSISKDLR SVWKPIAAII 900
    GRLNTLVISL AATNETFENI NSLFYLKWSA CTSLMDIIVA IKIFELKLPA 950
    DATTWRYSMS EQFPFYLHDN ASKVLLKIFL YLESLFAKQV DVQLERVADE 1000
    DANLNDLPET GFFENIETEF LLFTVKLKGL MKLNILDERF ASRVALNKEK 1050
    LGPLFKKIVD DTIMENPEPN KKNIQKAKSN QTQREKAPLQ PNSERETDHA 1100
    NTENNDPDIP MTIDLEPIEE SSQNNSELAP IEEHPTVVDA IDNSDEITQD 1150
    Length:1,150
    Mass (Da):133,009
    Last modified:February 1, 1995 - v1
    Checksum:i89688EA09485AC28
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti939 – 9391V → G in AAC49039. (PubMed:7483852)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17918 Genomic DNA. Translation: AAC49039.1.
    Z46881 Genomic DNA. Translation: CAA86966.1.
    BK006942 Genomic DNA. Translation: DAA08520.1.
    PIRiS49956.
    RefSeqiNP_012238.1. NM_001179376.1.

    Genome annotation databases

    EnsemblFungiiYIL026C; YIL026C; YIL026C.
    GeneIDi854786.
    KEGGisce:YIL026C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17918 Genomic DNA. Translation: AAC49039.1 .
    Z46881 Genomic DNA. Translation: CAA86966.1 .
    BK006942 Genomic DNA. Translation: DAA08520.1 .
    PIRi S49956.
    RefSeqi NP_012238.1. NM_001179376.1.

    3D structure databases

    ProteinModelPortali P40541.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34963. 28 interactions.
    DIPi DIP-5640N.
    IntActi P40541. 20 interactions.
    MINTi MINT-567741.
    STRINGi 4932.YIL026C.

    Proteomic databases

    MaxQBi P40541.
    PaxDbi P40541.
    PeptideAtlasi P40541.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL026C ; YIL026C ; YIL026C .
    GeneIDi 854786.
    KEGGi sce:YIL026C.

    Organism-specific databases

    CYGDi YIL026c.
    SGDi S000001288. IRR1.

    Phylogenomic databases

    eggNOGi COG5537.
    GeneTreei ENSGT00390000014094.
    HOGENOMi HOG000141696.
    KOi K06671.
    OMAi KYFGWLL.
    OrthoDBi EOG7QRR35.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31300-MONOMER.

    Miscellaneous databases

    NextBioi 977573.

    Gene expression databases

    Genevestigatori P40541.

    Family and domain databases

    InterProi IPR016024. ARM-type_fold.
    IPR020839. SCD.
    IPR013721. STAG.
    [Graphical view ]
    Pfami PF08514. STAG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 2 hits.
    PROSITEi PS51425. SCD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A new essential gene located on Saccharomyces cerevisiae chromosome IX."
      Kurlandzka A., Rytka J., Gromadka R., Murawski M.
      Yeast 11:885-890(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication."
      Toth A., Ciosk R., Uhlmann F., Galova M., Schleiffer A., Nasmyth K.
      Genes Dev. 13:320-333(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH MCD1, IDENTIFICATION IN A COHESIN COMPLEX WITH SMC1; SMC3 AND MCD1, INTERACTION OF THE COHESIN COMPLEX WITH SCC2.
    5. "Proteins interacting with Lin 1p, a putative link between chromosome segregation, mRNA splicing and DNA replication in Saccharomyces cerevisiae."
      Bialkowska A., Kurlandzka A.
      Yeast 19:1323-1333(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LIN1.
    6. "Molecular architecture of SMC proteins and the yeast cohesin complex."
      Haering C.H., Loewe J., Hochwagen A., Nasmyth K.
      Mol. Cell 9:773-788(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COHESIN COMPLEX WITH SMC1; SMC3 AND MCD1, STRUCTURE.
    7. "Eco1 is a novel acetyltransferase that can acetylate proteins involved in cohesion."
      Ivanov D., Schleiffer A., Eisenhaber F., Mechtler K., Haering C.H., Nasmyth K.
      Curr. Biol. 12:323-328(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28 AND SER-628, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSCC3_YEAST
    AccessioniPrimary (citable) accession number: P40541
    Secondary accession number(s): D6VVQ4, Q02511
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4090 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3