[Pyruvate dehydrogenase [lipoamide]] kinase, mitochondrial precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P40530 (PDK_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 90. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
[Pyruvate dehydrogenase [lipoamide]] kinase, mitochondrial
Short name=Pyruvate dehydrogenase kinase
EC=2.7.11.2

Alternative name(s):
Protein kinase of PDH protein 1

Gene names

Name:	PKP1
Ordered Locus Names:	YIL042C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. Also involved in telomere maintenance. Ref.6 Ref.7 Ref.8
Catalytic activity	ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate.
Subunit structure	Interacts with PKP2. Ref.8
Subcellular location	Mitochondrion matrix Ref.4 Ref.7 Ref.8.
Miscellaneous	Present with 4220 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the PDK/BCKDK protein kinase family. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro peptidyl-serine phosphorylation Inferred from mutant phenotype Ref.8. Source: SGD
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct pyruvate dehydrogenase (acetyl-transferring) kinase activity Inferred from mutant phenotype Ref.8. Source: SGD two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PKP2	P53170	3	EBI-2610722,EBI-23792

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – ?

Mitochondrion Potential

Chain

? – 394

[Pyruvate dehydrogenase [lipoamide]] kinase, mitochondrial

PRO_0000213686

Regions

Domain

126 – 386

261

Histidine kinase

Nucleotide binding

267 – 274

ATP By similarity

Nucleotide binding

323 – 324

ATP By similarity

Nucleotide binding

347 – 352

ATP By similarity

Sites

Binding site

304

ATP By similarity

Amino acid modifications

Modified residue

148

Phosphohistidine; by autocatalysis By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P40530 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: B2A996C089606961
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FASTA

394

45,444

        10         20         30         40         50         60 
MWKIMRSWKC GGMRWAHRQR PSHELLSQLS FDQHYKIRSN IELLIQDYAS KPIAPLNYEY 

        70         80         90        100        110        120 
FLQYRPPLTK KEEYMLTIKT INLLLSLTCK RLNAIQRLPY NAVINPHIER TNSLYLKSLQ 

       130        140        150        160        170        180 
TLLSIAYPYE LHNPPKIQAK FTELLDDHED AIVVLAKGLQ EIQSCYPKFQ ISQFLNFHLK 

       190        200        210        220        230        240 
ERITMKLLVT HYLSLMAQNK GDTNKRMIGI LHRDLPIAQL IKHVSDYVND ICFVKFNTQR 

       250        260        270        280        290        300 
TPVLIHPPSQ DITFTCIPPI LEYIMTEVFK NAFEAQIALG KEHMPIEINL LKPDDDELYL 

       310        320        330        340        350        360 
RIRDHGGGIT PEVEALMFNY SYSTHTQQSA DSESTDLPGE QINNVSGMGF GLPMCKTYLE 

       370        380        390 
LFGGKIDVQS LLGWGTDVYI KLKGPSKTAL LSKK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. Barrell B.G. Nature 387:84-87(1997) [PubMed: 9169870] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length." Askree S.H., Yehuda T., Smolikov S., Gurevich R., Hawk J., Coker C., Krauskopf A., Kupiec M., McEachern M.J. Proc. Natl. Acad. Sci. U.S.A. 101:8658-8663(2004) [PubMed: 15161972] [Abstract] Cited for: FUNCTION.
[7]	"YIL042c and YOR090c encode the kinase and phosphatase of the Saccharomyces cerevisiae pyruvate dehydrogenase complex." Krause-Buchholz U., Gey U., Wunschmann J., Becker S., Rodel G. FEBS Lett. 580:2553-2560(2006) [PubMed: 16643908] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"Yeast pyruvate dehydrogenase complex is regulated by a concerted activity of two kinases and two phosphatases." Gey U., Czupalla C., Hoflack B., Rodel G., Krause-Buchholz U. J. Biol. Chem. 283:9759-9767(2008) [PubMed: 18180296] [Abstract] Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH PKP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46861 Genomic DNA. Translation: CAA86909.1. AY692706 Genomic DNA. Translation: AAT92725.1. BK006942 Genomic DNA. Translation: DAA08506.1.
PIR	S50696.
RefSeq	NP_012222.1. NM_001179392.1.
3D structure databases
ProteinModelPortal	P40530.
SMR	P40530. Positions 55-385.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2547N.
IntAct	P40530. 2 interactions.
MINT	MINT-425189.
STRING	P40530.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL042C; YIL042C; YIL042C.
GeneID	854769.
KEGG	sce:YIL042C.
NMPDR	fig\|4932.3.peg.1755.
Organism-specific databases
CYGD	YIL042c.
SGD	S000001304. PKP1.
Phylogenomic databases
eggNOG	fuNOG05227.
GeneTree	EFGT00050000004593.
HOGENOM	HBG447614.
OMA	WGTDVYI.
OrthoDB	EOG486CNS.
Gene expression databases
ArrayExpress	P40530.
Genevestigator	P40530.
GermOnline	YIL042C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR018955. BCDHK/PDK_N. IPR004358. Sig_transdc_His_kin-like_C. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:1.20.140.20. BCDHK/PDK_N. 1 hit.
Pfam	PF10436. BCDHK_Adom3. 1 hit. PF02518. HATPase_c. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00387. HATPase_c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF69012. BCDHK/PDK_N. 1 hit.
PROSITE	PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977529.

Entry information

Entry name

PDK_YEAST

Accession

Primary (citable) accession number: P40530
Secondary accession number(s): D6VVP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents