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Protein

[Pyruvate dehydrogenase (acetyl-transferring)] kinase 1, mitochondrial

Gene

PKP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit (PDA1), thus contributing to the regulation of glucose metabolism. Also involved in telomere maintenance.3 Publications

Catalytic activityi

ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei304 – 3041ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi267 – 2748ATPBy similarity
Nucleotide bindingi323 – 3242ATPBy similarity
Nucleotide bindingi347 – 3526ATPBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: SGD
  • pyruvate dehydrogenase (acetyl-transferring) kinase activity Source: SGD

GO - Biological processi

  • carbon utilization Source: SGD
  • glucose metabolic process Source: UniProtKB-KW
  • peptidyl-serine phosphorylation Source: SGD
  • protein phosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31314-MONOMER.
ReactomeiR-SCE-204174. Regulation of pyruvate dehydrogenase (PDH) complex.
R-SCE-5362517. Signaling by Retinoic Acid.
R-SCE-70895. Branched-chain amino acid catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
[Pyruvate dehydrogenase (acetyl-transferring)] kinase 1, mitochondrial (EC:2.7.11.2)
Short name:
PDK 1
Short name:
Pyruvate dehydrogenase kinase 1
Alternative name(s):
Protein kinase of PDH protein 1
Pyruvate dehydrogenase complex kinase 1
Short name:
PDC kinase 1
[Pyruvate dehydrogenase [lipoamide]] kinase 1
Gene namesi
Name:PKP1
Ordered Locus Names:YIL042C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL042C.
SGDiS000001304. PKP1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2020MitochondrionSequence analysisAdd
BLAST
Chaini21 – 394374[Pyruvate dehydrogenase (acetyl-transferring)] kinase 1, mitochondrialPRO_0000213686Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei148 – 1481Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40530.

PTM databases

iPTMnetiP40530.

Interactioni

Subunit structurei

Interacts with PKP2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PKP2P531703EBI-2610722,EBI-23792

Protein-protein interaction databases

BioGridi34948. 31 interactions.
DIPiDIP-2547N.
IntActiP40530. 5 interactions.
MINTiMINT-425189.

Structurei

3D structure databases

ProteinModelPortaliP40530.
SMRiP40530. Positions 45-390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini126 – 386261Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PDK/BCKDK protein kinase family.Curated
Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00550000074574.
HOGENOMiHOG000115481.
InParanoidiP40530.
OMAiCETISPI.
OrthoDBiEOG7PZS7H.

Family and domain databases

Gene3Di1.20.140.20. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR018955. BCDHK/PDK_N.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF10436. BCDHK_Adom3. 1 hit.
PF02518. HATPase_c. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
SSF69012. SSF69012. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40530-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWKIMRSWKC GGMRWAHRQR PSHELLSQLS FDQHYKIRSN IELLIQDYAS
60 70 80 90 100
KPIAPLNYEY FLQYRPPLTK KEEYMLTIKT INLLLSLTCK RLNAIQRLPY
110 120 130 140 150
NAVINPHIER TNSLYLKSLQ TLLSIAYPYE LHNPPKIQAK FTELLDDHED
160 170 180 190 200
AIVVLAKGLQ EIQSCYPKFQ ISQFLNFHLK ERITMKLLVT HYLSLMAQNK
210 220 230 240 250
GDTNKRMIGI LHRDLPIAQL IKHVSDYVND ICFVKFNTQR TPVLIHPPSQ
260 270 280 290 300
DITFTCIPPI LEYIMTEVFK NAFEAQIALG KEHMPIEINL LKPDDDELYL
310 320 330 340 350
RIRDHGGGIT PEVEALMFNY SYSTHTQQSA DSESTDLPGE QINNVSGMGF
360 370 380 390
GLPMCKTYLE LFGGKIDVQS LLGWGTDVYI KLKGPSKTAL LSKK
Length:394
Mass (Da):45,444
Last modified:February 1, 1995 - v1
Checksum:iB2A996C089606961
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46861 Genomic DNA. Translation: CAA86909.1.
AY692706 Genomic DNA. Translation: AAT92725.1.
BK006942 Genomic DNA. Translation: DAA08506.1.
PIRiS50696.
RefSeqiNP_012222.1. NM_001179392.1.

Genome annotation databases

EnsemblFungiiYIL042C; YIL042C; YIL042C.
GeneIDi854769.
KEGGisce:YIL042C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46861 Genomic DNA. Translation: CAA86909.1.
AY692706 Genomic DNA. Translation: AAT92725.1.
BK006942 Genomic DNA. Translation: DAA08506.1.
PIRiS50696.
RefSeqiNP_012222.1. NM_001179392.1.

3D structure databases

ProteinModelPortaliP40530.
SMRiP40530. Positions 45-390.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34948. 31 interactions.
DIPiDIP-2547N.
IntActiP40530. 5 interactions.
MINTiMINT-425189.

PTM databases

iPTMnetiP40530.

Proteomic databases

MaxQBiP40530.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL042C; YIL042C; YIL042C.
GeneIDi854769.
KEGGisce:YIL042C.

Organism-specific databases

EuPathDBiFungiDB:YIL042C.
SGDiS000001304. PKP1.

Phylogenomic databases

GeneTreeiENSGT00550000074574.
HOGENOMiHOG000115481.
InParanoidiP40530.
OMAiCETISPI.
OrthoDBiEOG7PZS7H.

Enzyme and pathway databases

BioCyciYEAST:G3O-31314-MONOMER.
ReactomeiR-SCE-204174. Regulation of pyruvate dehydrogenase (PDH) complex.
R-SCE-5362517. Signaling by Retinoic Acid.
R-SCE-70895. Branched-chain amino acid catabolism.

Miscellaneous databases

PROiP40530.

Family and domain databases

Gene3Di1.20.140.20. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR018955. BCDHK/PDK_N.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF10436. BCDHK_Adom3. 1 hit.
PF02518. HATPase_c. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
SSF69012. SSF69012. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length."
    Askree S.H., Yehuda T., Smolikov S., Gurevich R., Hawk J., Coker C., Krauskopf A., Kupiec M., McEachern M.J.
    Proc. Natl. Acad. Sci. U.S.A. 101:8658-8663(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "YIL042c and YOR090c encode the kinase and phosphatase of the Saccharomyces cerevisiae pyruvate dehydrogenase complex."
    Krause-Buchholz U., Gey U., Wunschmann J., Becker S., Rodel G.
    FEBS Lett. 580:2553-2560(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. "Yeast pyruvate dehydrogenase complex is regulated by a concerted activity of two kinases and two phosphatases."
    Gey U., Czupalla C., Hoflack B., Rodel G., Krause-Buchholz U.
    J. Biol. Chem. 283:9759-9767(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH PKP2.

Entry informationi

Entry nameiPDK1_YEAST
AccessioniPrimary (citable) accession number: P40530
Secondary accession number(s): D6VVP0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 8, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4220 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.