ID   SYG1_YEAST              Reviewed;         902 AA.
AC   P40528; D6VVN4; P40964;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   27-MAR-2024, entry version 161.
DE   RecName: Full=Protein SYG1;
GN   Name=SYG1; OrderedLocusNames=YIL047C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP   INTERACTION WITH STE4.
RC   STRAIN=SP1;
RX   PubMed=7592711; DOI=10.1074/jbc.270.43.25435;
RA   Spain B.H., Koo D., Ramakrishnan M., Dzudzor B., Colicelli J.;
RT   "Truncated forms of a novel yeast protein suppress the lethality of a G
RT   protein alpha subunit deficiency by interacting with the beta subunit.";
RL   J. Biol. Chem. 270:25435-25444(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169870;
RA   Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA   Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA   Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA   Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA   Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL   Nature 387:84-87(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [5]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 208353 / W303-1A;
RX   PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA   Kim H., Melen K., Oesterberg M., von Heijne G.;
RT   "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-859 AND SER-860, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ADR376;
RX   PubMed=17330950; DOI=10.1021/pr060559j;
RA   Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA   Elias J.E., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of alpha-factor-arrested
RT   Saccharomyces cerevisiae.";
RL   J. Proteome Res. 6:1190-1197(2007).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-172 AND SER-179, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-859 AND SER-860, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- FUNCTION: May function in G-protein coupled signal transduction.
CC       {ECO:0000269|PubMed:7592711}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:7592711};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:7592711}.
CC   -!- MISCELLANEOUS: Present with 1160 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the SYG1 (TC 2.A.94) family. {ECO:0000305}.
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DR   EMBL; U14726; AAA91621.1; -; Genomic_DNA.
DR   EMBL; Z46861; CAA86904.1; -; Genomic_DNA.
DR   EMBL; BK006942; DAA08500.1; -; Genomic_DNA.
DR   PIR; S49931; S49931.
DR   RefSeq; NP_012217.3; NM_001179397.3.
DR   AlphaFoldDB; P40528; -.
DR   SMR; P40528; -.
DR   BioGRID; 34943; 121.
DR   DIP; DIP-2383N; -.
DR   IntAct; P40528; 2.
DR   MINT; P40528; -.
DR   STRING; 4932.YIL047C; -.
DR   iPTMnet; P40528; -.
DR   MaxQB; P40528; -.
DR   PaxDb; 4932-YIL047C; -.
DR   PeptideAtlas; P40528; -.
DR   EnsemblFungi; YIL047C_mRNA; YIL047C; YIL047C.
DR   GeneID; 854764; -.
DR   KEGG; sce:YIL047C; -.
DR   AGR; SGD:S000001309; -.
DR   SGD; S000001309; SYG1.
DR   VEuPathDB; FungiDB:YIL047C; -.
DR   eggNOG; KOG1162; Eukaryota.
DR   GeneTree; ENSGT00500000044895; -.
DR   HOGENOM; CLU_006116_1_1_1; -.
DR   InParanoid; P40528; -.
DR   OMA; SIPWAHA; -.
DR   OrthoDB; 7162at2759; -.
DR   BioCyc; YEAST:G3O-31318-MONOMER; -.
DR   BioGRID-ORCS; 854764; 4 hits in 10 CRISPR screens.
DR   PRO; PR:P40528; -.
DR   Proteomes; UP000002311; Chromosome IX.
DR   RNAct; P40528; Protein.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0000329; C:fungal-type vacuole membrane; HDA:SGD.
DR   GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR   GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR   GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR   GO; GO:0000822; F:inositol hexakisphosphate binding; IBA:GO_Central.
DR   GO; GO:0015114; F:phosphate ion transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0016036; P:cellular response to phosphate starvation; IBA:GO_Central.
DR   GO; GO:0006817; P:phosphate ion transport; IBA:GO_Central.
DR   GO; GO:0007165; P:signal transduction; IMP:SGD.
DR   CDD; cd14475; SPX_SYG1_like; 1.
DR   InterPro; IPR004342; EXS_C.
DR   InterPro; IPR004331; SPX_dom.
DR   PANTHER; PTHR10783:SF103; XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1; 1.
DR   PANTHER; PTHR10783; XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED; 1.
DR   Pfam; PF03124; EXS; 1.
DR   Pfam; PF03105; SPX; 1.
DR   PROSITE; PS51380; EXS; 1.
DR   PROSITE; PS51382; SPX; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..902
FT                   /note="Protein SYG1"
FT                   /id="PRO_0000072382"
FT   TOPO_DOM        1..404
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        405..425
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        426..435
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        436..456
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        457..497
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        498..518
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        519..522
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        523..543
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        544..554
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        555..575
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        576
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        577..599
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        600..732
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        733..753
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        754..761
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        762..782
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        783..902
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          1..303
FT                   /note="SPX"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00714"
FT   DOMAIN          606..815
FT                   /note="EXS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00712"
FT   REGION          882..902
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         172
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18407956"
FT   MOD_RES         179
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18407956"
FT   MOD_RES         859
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17330950,
FT                   ECO:0007744|PubMed:19779198"
FT   MOD_RES         860
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17330950,
FT                   ECO:0007744|PubMed:19779198"
SQ   SEQUENCE   902 AA;  104218 MW;  F8D87D1DDB3AED64 CRC64;
     MKFADHLTES AIPEWRDKYI DYKVGKKKLR RYKEKLDAEE EQSSSYRSWM PSVSVYQTAF
     QQREPGKSRS DGDYRSGPAF KKDYSALQRE FVADFIEDWL ISFQLSKCNE FYLWLLKECD
     KKFEVLQSQL HYYSLQKNYE RDNLNRSSSN VDMSTSLYAA GLAGRSDSRV NSIDSDSRSV
     MYGSMPCTKE AKKPRLSLLA YCQKVLKDNR LLPSWPKRGF SLLQDLRQDA SSRGRETFAF
     GASFLETMTT TQARNLLSNA IIEYYLYLQL VKSFRDINVT GFRKMVKKFD KTCHTRELTT
     FMSYARTHYT LFKHADANVQ LVAQKMQQIT SSQPTPTSEL SSAQRDKEPI TWLETQITEW
     FTTALTNSPK DRKHNTHKLK KLTIQYSISE QMVHRNNRSI VQMLVVGLGI GVSMTLITYT
     LYLGISSEET SFTHKILFPL WGGWYMVLLI AFLFLVNCFI WHRTGINYRF IMLGEIQSKN
     GTQFFNNDFA TSKIPLKLYF LTFFIVPCAV CSMLSFALEK LTPLGFLYIG IVSFLFLCPS
     GLIPYWDKVV HTRKWLVVTL IRLMMSGFFP VEFGDFFLGD IICSLTYSIA DIAMFFCVYS
     HTPNNLCGSS HSRAMGVLSC LPSYWRFMQC LRRFADSGDW FPHLLNAAKY TLGIAYNATL
     CAYRLSDRSE QRRTPFIVCA TLNSILTSAW DLVMDWSFAH NTTSYNWLLR DDLYLAGKKN
     WENGSYSFSR KLVYYFAMIW DILIRFEWIV YAIAPQTIQQ SAVTSFILAL LEVLRRFVWI
     IFRVENEHVA NVHLFRVTGD APLPYPIAQV GDDSMDSSDL GSKAFSSLND IPITPSHDNN
     PHSFAEPMPA YRGTFRRRSS VFENISRSIP WAHATDFQRP TVNTVDDRSP ETDSESEVES
     IM
//