Protein SYG1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P40528 (SYG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein SYG1

Gene names

Name:	SYG1
Ordered Locus Names:	YIL047C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	902 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function in G-protein coupled signal transduction. Ref.1
Subcellular location	Cell membrane; Multi-pass membrane protein Ref.1.
Miscellaneous	Present with 1160 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SYG1 (TC 2.A.94) family. [View classification] Contains 1 EXS domain. Contains 1 SPX domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	signal transduction Inferred from mutant phenotype Ref.1. Source: SGD
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD mitochondrion Inferred from direct assay PubMed 14576278 PubMed 16823961. Source: SGD plasma membrane Inferred from direct assay Ref.1. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-18696,EBI-18696
FTH1	P38310	1	EBI-18696,EBI-20959

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 902

902

Protein SYG1

PRO_0000072382

Regions

Topological domain

1 – 404

404

Cytoplasmic Potential

Transmembrane

405 – 425

Helical; Potential

Topological domain

426 – 435

Extracellular Potential

Transmembrane

436 – 456

Helical; Potential

Topological domain

457 – 497

Cytoplasmic Potential

Transmembrane

498 – 518

Helical; Potential

Topological domain

519 – 522

Extracellular Potential

Transmembrane

523 – 543

Helical; Potential

Topological domain

544 – 554

Cytoplasmic Potential

Transmembrane

555 – 575

Helical; Potential

Topological domain

576

Extracellular Potential

Transmembrane

577 – 599

Helical; Potential

Topological domain

600 – 732

133

Cytoplasmic Potential

Transmembrane

733 – 753

Helical; Potential

Topological domain

754 – 761

Extracellular Potential

Transmembrane

762 – 782

Helical; Potential

Topological domain

783 – 902

120

Cytoplasmic Potential

Domain

1 – 303

303

SPX

Domain

606 – 815

210

EXS

Amino acid modifications

Modified residue

Phosphoserine Ref.9

Modified residue

149

Phosphoserine Ref.6 Ref.9

Modified residue

172

Phosphoserine Ref.9

Modified residue

179

Phosphoserine Ref.9

Modified residue

859

Phosphoserine Ref.4 Ref.8 Ref.9

Modified residue

860

Phosphoserine Ref.4 Ref.8 Ref.9

Sequences

Sequence

Length

Mass (Da)

Tools

P40528 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: F8D87D1DDB3AED64
Show »

FASTA

902

104,218

        10         20         30         40         50         60 
MKFADHLTES AIPEWRDKYI DYKVGKKKLR RYKEKLDAEE EQSSSYRSWM PSVSVYQTAF 

        70         80         90        100        110        120 
QQREPGKSRS DGDYRSGPAF KKDYSALQRE FVADFIEDWL ISFQLSKCNE FYLWLLKECD 

       130        140        150        160        170        180 
KKFEVLQSQL HYYSLQKNYE RDNLNRSSSN VDMSTSLYAA GLAGRSDSRV NSIDSDSRSV 

       190        200        210        220        230        240 
MYGSMPCTKE AKKPRLSLLA YCQKVLKDNR LLPSWPKRGF SLLQDLRQDA SSRGRETFAF 

       250        260        270        280        290        300 
GASFLETMTT TQARNLLSNA IIEYYLYLQL VKSFRDINVT GFRKMVKKFD KTCHTRELTT 

       310        320        330        340        350        360 
FMSYARTHYT LFKHADANVQ LVAQKMQQIT SSQPTPTSEL SSAQRDKEPI TWLETQITEW 

       370        380        390        400        410        420 
FTTALTNSPK DRKHNTHKLK KLTIQYSISE QMVHRNNRSI VQMLVVGLGI GVSMTLITYT 

       430        440        450        460        470        480 
LYLGISSEET SFTHKILFPL WGGWYMVLLI AFLFLVNCFI WHRTGINYRF IMLGEIQSKN 

       490        500        510        520        530        540 
GTQFFNNDFA TSKIPLKLYF LTFFIVPCAV CSMLSFALEK LTPLGFLYIG IVSFLFLCPS 

       550        560        570        580        590        600 
GLIPYWDKVV HTRKWLVVTL IRLMMSGFFP VEFGDFFLGD IICSLTYSIA DIAMFFCVYS 

       610        620        630        640        650        660 
HTPNNLCGSS HSRAMGVLSC LPSYWRFMQC LRRFADSGDW FPHLLNAAKY TLGIAYNATL 

       670        680        690        700        710        720 
CAYRLSDRSE QRRTPFIVCA TLNSILTSAW DLVMDWSFAH NTTSYNWLLR DDLYLAGKKN 

       730        740        750        760        770        780 
WENGSYSFSR KLVYYFAMIW DILIRFEWIV YAIAPQTIQQ SAVTSFILAL LEVLRRFVWI 

       790        800        810        820        830        840 
IFRVENEHVA NVHLFRVTGD APLPYPIAQV GDDSMDSSDL GSKAFSSLND IPITPSHDNN 

       850        860        870        880        890        900 
PHSFAEPMPA YRGTFRRRSS VFENISRSIP WAHATDFQRP TVNTVDDRSP ETDSESEVES 


IM

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Truncated forms of a novel yeast protein suppress the lethality of a G protein alpha subunit deficiency by interacting with the beta subunit." Spain B.H., Koo D., Ramakrishnan M., Dzudzor B., Colicelli J. J. Biol. Chem. 270:25435-25444(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH STE4. Strain: SP1.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-859 AND SER-860, MASS SPECTROMETRY. Strain: 2124.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-149, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-859 AND SER-860, MASS SPECTROMETRY. Strain: ADR376.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44; SER-149; SER-172; SER-179; SER-859 AND SER-860, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U14726 Genomic DNA. Translation: AAA91621.1. Z46861 Genomic DNA. Translation: CAA86904.1. BK006942 Genomic DNA. Translation: DAA08500.1.
PIR	S49931.
RefSeq	NP_012217.3. NM_001179397.3.
3D structure databases
ProteinModelPortal	P40528.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2383N.
IntAct	P40528. 2 interactions.
MINT	MINT-599154.
STRING	4932.YIL047C.
Proteomic databases
PaxDb	P40528.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL047C; YIL047C; YIL047C.
GeneID	854764.
KEGG	sce:YIL041W. sce:YIL047C.
Organism-specific databases
CYGD	YIL047c.
SGD	S000001309. SYG1.
Phylogenomic databases
eggNOG	COG5408.
GeneTree	ENSGT00500000044895.
HOGENOM	HOG000141697.
OMA	INYRFIM.
OrthoDB	EOG4Z39PR.
Gene expression databases
Genevestigator	P40528.
GermOnline	YIL047C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004342. EXS_C. IPR004331. SPX_N. [Graphical view]
Pfam	PF03124. EXS. 1 hit. PF03105. SPX. 1 hit. [Graphical view]
PROSITE	PS51380. EXS. 1 hit. PS51382. SPX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977514.

Entry information

Entry name

SYG1_YEAST

Accession

Primary (citable) accession number: P40528
Secondary accession number(s): D6VVN4, P40964

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents