P40527 (ATC7_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 117.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Probable phospholipid-transporting ATPase NEO1 EC=3.6.3.1 | ||||
| Gene names |
| ||||
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | ||||
| Taxonomic identifier | 559292 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 1151 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids Potential. Leads to neomycin-resistance when overexpressed. Required for traffic between late Golgi and early endosomes. Ref.3 |
| Catalytic activity | ATP + H2O + phospholipid(In) = ADP + phosphate + phospholipid(Out). |
| Subunit structure | Interacts with MON2. Ref.3 |
| Subcellular location | Endosome membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein Ref.3. |
| Sequence similarities | Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. [View classification] |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1151 | 1151 | Probable phospholipid-transporting ATPase NEO1 | PRO_0000046236 | |||||
Regions | |||||||||
| Topological domain | 1 – 184 | 184 | Extracellular Potential | ||||||
| Transmembrane | 185 – 205 | 21 | Helical; Potential | ||||||
| Topological domain | 206 – 209 | 4 | Cytoplasmic Potential | ||||||
| Transmembrane | 210 – 230 | 21 | Helical; Potential | ||||||
| Topological domain | 231 – 367 | 137 | Extracellular Potential | ||||||
| Transmembrane | 368 – 388 | 21 | Helical; Potential | ||||||
| Topological domain | 389 – 416 | 28 | Cytoplasmic Potential | ||||||
| Transmembrane | 417 – 437 | 21 | Helical; Potential | ||||||
| Topological domain | 438 | 1 | Extracellular Potential | ||||||
| Transmembrane | 439 – 459 | 21 | Helical; Potential | ||||||
| Topological domain | 460 – 947 | 488 | Cytoplasmic Potential | ||||||
| Transmembrane | 948 – 968 | 21 | Helical; Potential | ||||||
| Topological domain | 969 – 970 | 2 | Extracellular Potential | ||||||
| Transmembrane | 971 – 991 | 21 | Helical; Potential | ||||||
| Topological domain | 992 – 1020 | 29 | Cytoplasmic Potential | ||||||
| Transmembrane | 1021 – 1041 | 21 | Helical; Potential | ||||||
| Topological domain | 1042 – 1052 | 11 | Extracellular Potential | ||||||
| Transmembrane | 1053 – 1073 | 21 | Helical; Potential | ||||||
| Topological domain | 1074 – 1078 | 5 | Cytoplasmic Potential | ||||||
| Transmembrane | 1079 – 1099 | 21 | Helical; Potential | ||||||
| Topological domain | 1100 – 1109 | 10 | Extracellular Potential | ||||||
| Transmembrane | 1110 – 1130 | 21 | Helical; Potential | ||||||
| Topological domain | 1131 – 1151 | 21 | Cytoplasmic Potential | ||||||
| Region | 1131 – 1151 | 21 | Required for endosomal targeting | ||||||
Sites | |||||||||
| Active site | 503 | 1 | 4-aspartylphosphate intermediate Probable | ||||||
Amino acid modifications | |||||||||
| Modified residue | 64 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 102 | 1 | Phosphoserine Ref.5 Ref.7 | ||||||
| Modified residue | 115 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 551 | 1 | Phosphoserine Ref.6 Ref.7 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. Barrell B.G.Nature 387:84-87(1997) [PubMed: 9169870] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [2] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [3] | "Molecular interactions of yeast Neo1p, an essential member of the Drs2 family of aminophospholipid translocases, and its role in membrane trafficking within the endomembrane system." Wicky S., Schwarz H., Singer-Krueger B. Mol. Cell. Biol. 24:7402-7418(2004) [PubMed: 15314152] [Abstract] Cited for: INTERACTION WITH MON2, SUBCELLULAR LOCATION, FUNCTION. |
| [4] | "A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A. |
| [5] | "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102, MASS SPECTROMETRY. Strain: ADR376. |
| [6] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-551, MASS SPECTROMETRY. |
| [7] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64; SER-102; SER-115 AND SER-551, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Z38060 Genomic DNA. Translation: CAA86174.1. BK006942 Genomic DNA. Translation: DAA08499.1. |
| PIR | S48431. |
| RefSeq | NP_012216.1. NM_001179398.1. |
3D structure databases | |
| ProteinModelPortal | P40527. |
| SMR | P40527. Positions 854-917. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-2548N. |
| IntAct | P40527. 2 interactions. |
| MINT | MINT-422616. |
| STRING | P40527. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | YIL048W; YIL048W; YIL048W. |
| GeneID | 854763. |
| KEGG | sce:YIL048W. |
| NMPDR | fig|4932.3.peg.1747. |
Organism-specific databases | |
| CYGD | YIL048w. |
| SGD | S000001310. NEO1. |
Phylogenomic databases | |
| eggNOG | fuNOG05222. |
| GeneTree | EFGT00050000000755. |
| HOGENOM | HBG720821. |
| OMA | RRTCAIG. |
| OrthoDB | EOG49KJZN. |
Gene expression databases | |
| ArrayExpress | P40527. |
| Genevestigator | P40527. |
| GermOnline | YIL048W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR023306. ATPase_cation_domN. IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR023300. ATPase_P-typ_cyto_domA. IPR023299. ATPase_P-typ_cyto_domN. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR006539. ATPase_P-typ_Plipid-transl. IPR023214. HAD-like_dom. [Graphical view] |
| Gene3D | G3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 2 hits. G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 1 hit. G3DSA:3.40.50.1000. HAD-like_dom. 2 hits. |
| KO | K01530. |
| Pfam | PF00122. E1-E2_ATPase. 1 hit. [Graphical view] |
| PRINTS | PR00119. CATATPASE. |
| SUPFAM | SSF81660. ATPase_cation_domN. 1 hit. SSF56784. HAD-like_dom. 1 hit. |
| TIGRFAMs | TIGR01652. ATPase-Plipid. 1 hit. TIGR01494. ATPase_P-type. 3 hits. |
| PROSITE | PS00154. ATPASE_E1_E2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 977511. |
Entry information
| Entry name | ATC7_YEAST | ||||||||
| Accession | Primary (citable) accession number: P40527 Secondary accession number(s): D6VVN3 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome IX Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with