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Protein

Probable phospholipid-transporting ATPase NEO1

Gene

NEO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids (Potential). Leads to neomycin-resistance when overexpressed. Required for traffic between late Golgi and early endosomes.Curated1 Publication

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei503 – 50314-aspartylphosphate intermediateCurated

GO - Molecular functioni

  • ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Source: SGD
  • ATP binding Source: UniProtKB-KW
  • magnesium ion binding Source: InterPro
  • phospholipid-translocating ATPase activity Source: SGD

GO - Biological processi

  • endocytosis Source: SGD
  • ion transmembrane transport Source: GOC
  • phospholipid translocation Source: GOC
  • protein transport Source: UniProtKB-KW
  • retrograde vesicle-mediated transport, Golgi to ER Source: SGD
  • vacuole organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31319-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phospholipid-transporting ATPase NEO1 (EC:3.6.3.1)
Gene namesi
Name:NEO1
Ordered Locus Names:YIL048W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL048W.
SGDiS000001310. NEO1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 184184ExtracellularSequence analysisAdd
BLAST
Transmembranei185 – 20521HelicalSequence analysisAdd
BLAST
Topological domaini206 – 2094CytoplasmicSequence analysis
Transmembranei210 – 23021HelicalSequence analysisAdd
BLAST
Topological domaini231 – 367137ExtracellularSequence analysisAdd
BLAST
Transmembranei368 – 38821HelicalSequence analysisAdd
BLAST
Topological domaini389 – 41628CytoplasmicSequence analysisAdd
BLAST
Transmembranei417 – 43721HelicalSequence analysisAdd
BLAST
Topological domaini438 – 4381ExtracellularSequence analysis
Transmembranei439 – 45921HelicalSequence analysisAdd
BLAST
Topological domaini460 – 947488CytoplasmicSequence analysisAdd
BLAST
Transmembranei948 – 96821HelicalSequence analysisAdd
BLAST
Topological domaini969 – 9702ExtracellularSequence analysis
Transmembranei971 – 99121HelicalSequence analysisAdd
BLAST
Topological domaini992 – 102029CytoplasmicSequence analysisAdd
BLAST
Transmembranei1021 – 104121HelicalSequence analysisAdd
BLAST
Topological domaini1042 – 105211ExtracellularSequence analysisAdd
BLAST
Transmembranei1053 – 107321HelicalSequence analysisAdd
BLAST
Topological domaini1074 – 10785CytoplasmicSequence analysis
Transmembranei1079 – 109921HelicalSequence analysisAdd
BLAST
Topological domaini1100 – 110910ExtracellularSequence analysis
Transmembranei1110 – 113021HelicalSequence analysisAdd
BLAST
Topological domaini1131 – 115121CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endosome Source: SGD
  • endosome membrane Source: UniProtKB-SubCell
  • Golgi apparatus Source: SGD
  • Golgi membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11511151Probable phospholipid-transporting ATPase NEO1PRO_0000046236Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei102 – 1021PhosphoserineCombined sources
Modified residuei551 – 5511PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40527.
PRIDEiP40527.

PTM databases

iPTMnetiP40527.

Interactioni

Subunit structurei

Interacts with MON2.1 Publication

Protein-protein interaction databases

BioGridi34942. 93 interactions.
DIPiDIP-2548N.
IntActiP40527. 6 interactions.
MINTiMINT-422616.

Structurei

3D structure databases

ProteinModelPortaliP40527.
SMRiP40527. Positions 265-912.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1131 – 115121Required for endosomal targetingAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074723.
HOGENOMiHOG000201571.
InParanoidiP40527.
KOiK01530.
OMAiELLLWHG.
OrthoDBiEOG7FR7QR.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40527-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNPPSFKSH KQNLFNSNNN QHANSVDSFD LHLDDSFDAA LDSLQINNNP
60 70 80 90 100
EPLSKHNTVG DRESFEMRTV DDLDNFSNHS SDSHRKSSNT DTHPLMYDNR
110 120 130 140 150
LSQDDNFKFT NIASSPPSSS NNIFSKALSY LKVSNTKNWS KFGSPIELSD
160 170 180 190 200
QHIEREIHPD TTPVYDRNRY VSNELSNAKY NAVTFVPTLL YEQFKFFYNL
210 220 230 240 250
YFLVVALSQA VPALRIGYLS SYIVPLAFVL TVTMAKEAID DIQRRRRDRE
260 270 280 290 300
SNNELYHVIT RNRSIPSKDL KVGDLIKVHK GDRIPADLVL LQSSEPSGES
310 320 330 340 350
FIKTDQLDGE TDWKLRVACP LTQNLSENDL INRISITASA PEKSIHKFLG
360 370 380 390 400
KVTYKDSTSN PLSVDNTLWA NTVLASSGFC IACVVYTGRD TRQAMNTTTA
410 420 430 440 450
KVKTGLLELE INSISKILCA CVFALSILLV AFAGFHNDDW YIDILRYLIL
460 470 480 490 500
FSTIIPVSLR VNLDLAKSVY AHQIEHDKTI PETIVRTSTI PEDLGRIEYL
510 520 530 540 550
LSDKTGTLTQ NDMQLKKIHL GTVSYTSETL DIVSDYVQSL VSSKNDSLNN
560 570 580 590 600
SKVALSTTRK DMSFRVRDMI LTLAICHNVT PTFEDDELTY QAASPDEIAI
610 620 630 640 650
VKFTESVGLS LFKRDRHSIS LLHEHSGKTL NYEILQVFPF NSDSKRMGII
660 670 680 690 700
VRDEQLDEYW FMQKGADTVM SKIVESNDWL EEETGNMARE GLRTLVIGRK
710 720 730 740 750
KLNKKIYEQF QKEYNDASLS MLNRDQQMSQ VITKYLEHDL ELLGLTGVED
760 770 780 790 800
KLQKDVKSSI ELLRNAGIKI WMLTGDKVET ARCVSISAKL ISRGQYVHTI
810 820 830 840 850
TKVTRPEGAF NQLEYLKINR NACLLIDGES LGMFLKHYEQ EFFDVVVHLP
860 870 880 890 900
TVIACRCTPQ QKADVALVIR KMTGKRVCCI GDGGNDVSMI QCADVGVGIV
910 920 930 940 950
GKEGKQASLA ADFSITQFCH LTELLLWHGR NSYKRSAKLA QFVMHRGLII
960 970 980 990 1000
AICQAVYSIC SLFEPIALYQ GWLMVGYATC YTMAPVFSLT LDHDIEESLT
1010 1020 1030 1040 1050
KIYPELYKEL TEGKSLSYKT FFVWVLLSLF QGSVIQLFSQ AFTSLLDTDF
1060 1070 1080 1090 1100
TRMVAISFTA LVVNELIMVA LEIYTWNKTM LVTEIATLLF YIVSVPFLGD
1110 1120 1130 1140 1150
YFDLGYMTTV NYYAGLLVIL LISIFPVWTA KAIYRRLHPP SYAKVQEFAT

P
Length:1,151
Mass (Da):130,218
Last modified:February 1, 1995 - v1
Checksum:iDC7225CC9577DBE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38060 Genomic DNA. Translation: CAA86174.1.
BK006942 Genomic DNA. Translation: DAA08499.1.
PIRiS48431.
RefSeqiNP_012216.1. NM_001179398.1.

Genome annotation databases

EnsemblFungiiYIL048W; YIL048W; YIL048W.
GeneIDi854763.
KEGGisce:YIL048W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38060 Genomic DNA. Translation: CAA86174.1.
BK006942 Genomic DNA. Translation: DAA08499.1.
PIRiS48431.
RefSeqiNP_012216.1. NM_001179398.1.

3D structure databases

ProteinModelPortaliP40527.
SMRiP40527. Positions 265-912.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34942. 93 interactions.
DIPiDIP-2548N.
IntActiP40527. 6 interactions.
MINTiMINT-422616.

PTM databases

iPTMnetiP40527.

Proteomic databases

MaxQBiP40527.
PRIDEiP40527.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL048W; YIL048W; YIL048W.
GeneIDi854763.
KEGGisce:YIL048W.

Organism-specific databases

EuPathDBiFungiDB:YIL048W.
SGDiS000001310. NEO1.

Phylogenomic databases

GeneTreeiENSGT00550000074723.
HOGENOMiHOG000201571.
InParanoidiP40527.
KOiK01530.
OMAiELLLWHG.
OrthoDBiEOG7FR7QR.

Enzyme and pathway databases

BioCyciYEAST:G3O-31319-MONOMER.

Miscellaneous databases

PROiP40527.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Molecular interactions of yeast Neo1p, an essential member of the Drs2 family of aminophospholipid translocases, and its role in membrane trafficking within the endomembrane system."
    Wicky S., Schwarz H., Singer-Krueger B.
    Mol. Cell. Biol. 24:7402-7418(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MON2, SUBCELLULAR LOCATION, FUNCTION.
  4. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102 AND SER-551, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiATC7_YEAST
AccessioniPrimary (citable) accession number: P40527
Secondary accession number(s): D6VVN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 8, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.