Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Polyprenol reductase

Gene

DFG10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism.1 Publication

Catalytic activityi

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.1 Publication

Pathwayi

GO - Molecular functioni

  1. 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: SGD

GO - Biological processi

  1. dolichol biosynthetic process Source: SGD
  2. dolichol-linked oligosaccharide biosynthetic process Source: UniProtKB
  3. dolichol metabolic process Source: UniProtKB
  4. polyprenol catabolic process Source: UniProtKB
  5. protein glycosylation Source: UniProtKB-UniPathway
  6. pseudohyphal growth Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciYEAST:G3O-31320-MONOMER.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyprenol reductase (EC:1.3.1.94)
Alternative name(s):
Protein DFG10
Gene namesi
Name:DFG10
Ordered Locus Names:YIL049W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIL049w.
SGDiS000001311. DFG10.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei18 – 3821HelicalSequence AnalysisAdd
BLAST
Transmembranei78 – 9821HelicalSequence AnalysisAdd
BLAST
Transmembranei123 – 14321HelicalSequence AnalysisAdd
BLAST
Transmembranei200 – 22021HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Suppression of filamentatous growth, defects in cell polarity, and cellular elongation, due to defects in N-glycosylation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 253253Polyprenol reductasePRO_0000213682Add
BLAST

Proteomic databases

MaxQBiP40526.

Expressioni

Gene expression databases

GenevestigatoriP40526.

Interactioni

Protein-protein interaction databases

BioGridi34941. 13 interactions.
DIPiDIP-4658N.
MINTiMINT-512904.
STRINGi4932.YIL049W.

Structurei

3D structure databases

ProteinModelPortaliP40526.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG330066.
HOGENOMiHOG000248191.
InParanoidiP40526.
KOiK12345.
OMAiTHYYILA.
OrthoDBiEOG7H1JX2.

Family and domain databases

InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40526-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYFDEEQLLK YTIYAYRLSF FVGICSLFIA KSCLPEFLQY GKTYRPKENS
60 70 80 90 100
KYSSILERIK KFTVPKAYFS HFYYLATFLS LVTLYFYPKF PIVWIIFGHS
110 120 130 140 150
LRRLYETLYV LHYTSNSRMN WSHYLVGIWF YSVLLLILNI SLYKNSIPNT
160 170 180 190 200
LNMNAFIIFC IASWDQYKNH VILANLVKYS LPTGRLFRLV CCPHYLDEII
210 220 230 240 250
IYSTLLPYEQ EFYLTLVWVI TSLTISALET KNYYRHKFKD NHVAPYAIIP

FII
Length:253
Mass (Da):30,306
Last modified:February 1, 1995 - v1
Checksum:iFBADD31A3BC5B054
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38060 Genomic DNA. Translation: CAA86173.1.
BK006942 Genomic DNA. Translation: DAA08498.1.
PIRiS48430.
RefSeqiNP_012215.1. NM_001179399.1.

Genome annotation databases

EnsemblFungiiYIL049W; YIL049W; YIL049W.
GeneIDi854762.
KEGGisce:YIL049W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38060 Genomic DNA. Translation: CAA86173.1.
BK006942 Genomic DNA. Translation: DAA08498.1.
PIRiS48430.
RefSeqiNP_012215.1. NM_001179399.1.

3D structure databases

ProteinModelPortaliP40526.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34941. 13 interactions.
DIPiDIP-4658N.
MINTiMINT-512904.
STRINGi4932.YIL049W.

Proteomic databases

MaxQBiP40526.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL049W; YIL049W; YIL049W.
GeneIDi854762.
KEGGisce:YIL049W.

Organism-specific databases

CYGDiYIL049w.
SGDiS000001311. DFG10.

Phylogenomic databases

eggNOGiNOG330066.
HOGENOMiHOG000248191.
InParanoidiP40526.
KOiK12345.
OMAiTHYYILA.
OrthoDBiEOG7H1JX2.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciYEAST:G3O-31320-MONOMER.

Miscellaneous databases

NextBioi977508.
PROiP40526.

Gene expression databases

GenevestigatoriP40526.

Family and domain databases

InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Dissection of filamentous growth by transposon mutagenesis in Saccharomyces cerevisiae."
    Moesch H.-U., Fink G.R.
    Genetics 145:671-684(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME, DISRUPTION PHENOTYPE.
  4. Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiDFG10_YEAST
AccessioniPrimary (citable) accession number: P40526
Secondary accession number(s): D6VVN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: January 7, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.