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P40526

- DFG10_YEAST

UniProt

P40526 - DFG10_YEAST

Protein

Polyprenol reductase

Gene

DFG10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism.1 Publication

    Catalytic activityi

    Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: SGD

    GO - Biological processi

    1. dolichol biosynthetic process Source: SGD
    2. dolichol-linked oligosaccharide biosynthetic process Source: UniProtKB
    3. dolichol metabolic process Source: UniProtKB
    4. polyprenol catabolic process Source: UniProtKB
    5. protein glycosylation Source: UniProtKB-UniPathway
    6. pseudohyphal growth Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31320-MONOMER.
    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyprenol reductase (EC:1.3.1.94)
    Alternative name(s):
    Protein DFG10
    Gene namesi
    Name:DFG10
    Ordered Locus Names:YIL049W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL049w.
    SGDiS000001311. DFG10.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Suppression of filamentatous growth, defects in cell polarity, and cellular elongation, due to defects in N-glycosylation.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 253253Polyprenol reductasePRO_0000213682Add
    BLAST

    Proteomic databases

    MaxQBiP40526.

    Expressioni

    Gene expression databases

    GenevestigatoriP40526.

    Interactioni

    Protein-protein interaction databases

    BioGridi34941. 13 interactions.
    DIPiDIP-4658N.
    MINTiMINT-512904.
    STRINGi4932.YIL049W.

    Structurei

    3D structure databases

    ProteinModelPortaliP40526.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei18 – 3821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei78 – 9821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei123 – 14321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei200 – 22021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG330066.
    HOGENOMiHOG000248191.
    KOiK12345.
    OMAiLVGIWFY.
    OrthoDBiEOG7H1JX2.

    Family and domain databases

    InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view]
    PfamiPF02544. Steroid_dh. 1 hit.
    [Graphical view]
    PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40526-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYFDEEQLLK YTIYAYRLSF FVGICSLFIA KSCLPEFLQY GKTYRPKENS    50
    KYSSILERIK KFTVPKAYFS HFYYLATFLS LVTLYFYPKF PIVWIIFGHS 100
    LRRLYETLYV LHYTSNSRMN WSHYLVGIWF YSVLLLILNI SLYKNSIPNT 150
    LNMNAFIIFC IASWDQYKNH VILANLVKYS LPTGRLFRLV CCPHYLDEII 200
    IYSTLLPYEQ EFYLTLVWVI TSLTISALET KNYYRHKFKD NHVAPYAIIP 250
    FII 253
    Length:253
    Mass (Da):30,306
    Last modified:February 1, 1995 - v1
    Checksum:iFBADD31A3BC5B054
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z38060 Genomic DNA. Translation: CAA86173.1.
    BK006942 Genomic DNA. Translation: DAA08498.1.
    PIRiS48430.
    RefSeqiNP_012215.1. NM_001179399.1.

    Genome annotation databases

    EnsemblFungiiYIL049W; YIL049W; YIL049W.
    GeneIDi854762.
    KEGGisce:YIL049W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z38060 Genomic DNA. Translation: CAA86173.1 .
    BK006942 Genomic DNA. Translation: DAA08498.1 .
    PIRi S48430.
    RefSeqi NP_012215.1. NM_001179399.1.

    3D structure databases

    ProteinModelPortali P40526.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34941. 13 interactions.
    DIPi DIP-4658N.
    MINTi MINT-512904.
    STRINGi 4932.YIL049W.

    Proteomic databases

    MaxQBi P40526.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL049W ; YIL049W ; YIL049W .
    GeneIDi 854762.
    KEGGi sce:YIL049W.

    Organism-specific databases

    CYGDi YIL049w.
    SGDi S000001311. DFG10.

    Phylogenomic databases

    eggNOGi NOG330066.
    HOGENOMi HOG000248191.
    KOi K12345.
    OMAi LVGIWFY.
    OrthoDBi EOG7H1JX2.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BioCyci YEAST:G3O-31320-MONOMER.

    Miscellaneous databases

    NextBioi 977508.
    PROi P40526.

    Gene expression databases

    Genevestigatori P40526.

    Family and domain databases

    InterProi IPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view ]
    Pfami PF02544. Steroid_dh. 1 hit.
    [Graphical view ]
    PROSITEi PS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Dissection of filamentous growth by transposon mutagenesis in Saccharomyces cerevisiae."
      Moesch H.-U., Fink G.R.
      Genetics 145:671-684(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE NAME, DISRUPTION PHENOTYPE.
    4. Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiDFG10_YEAST
    AccessioniPrimary (citable) accession number: P40526
    Secondary accession number(s): D6VVN2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3