P40526 (DFG10_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polyprenol reductase
EC=1.3.1.94

Alternative name(s):
Protein DFG10

Gene names

Name:	DFG10
Ordered Locus Names:	YIL049W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Ref.4
Catalytic activity	Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH. Ref.4
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Disruption phenotype	Suppression of filamentatous growth, defects in cell polarity, and cellular elongation, due to defects in N-glycosylation. Ref.3 Ref.4
Sequence similarities	Belongs to the steroid 5-alpha reductase family. Polyprenol reductase subfamily.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dolichol biosynthetic process Inferred from mutant phenotype Ref.4. Source: SGD dolichol-linked oligosaccharide biosynthetic process Inferred from mutant phenotype Ref.4. Source: UniProtKB polyprenol catabolic process Inferred from mutant phenotype Ref.4. Source: UniProtKB protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway pseudohyphal growth Inferred from mutant phenotype Ref.3. Source: SGD
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD
Molecular_function	3-oxo-5-alpha-steroid 4-dehydrogenase activity Inferred from sequence or structural similarity Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Polyprenol reductase		PRO_0000213682
Regions
	Transmembrane	18 – 38	21	Helical; Potential
	Transmembrane	78 – 98	21	Helical; Potential
	Transmembrane	123 – 143	21	Helical; Potential
	Transmembrane	200 – 220	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P40526 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: FBADD31A3BC5B054	FASTA	253	30,306
	10 20 30 40 50 60 MYFDEEQLLK YTIYAYRLSF FVGICSLFIA KSCLPEFLQY GKTYRPKENS KYSSILERIK 70 80 90 100 110 120 KFTVPKAYFS HFYYLATFLS LVTLYFYPKF PIVWIIFGHS LRRLYETLYV LHYTSNSRMN 130 140 150 160 170 180 WSHYLVGIWF YSVLLLILNI SLYKNSIPNT LNMNAFIIFC IASWDQYKNH VILANLVKYS 190 200 210 220 230 240 LPTGRLFRLV CCPHYLDEII IYSTLLPYEQ EFYLTLVWVI TSLTISALET KNYYRHKFKD 250 NHVAPYAIIP FII

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Dissection of filamentous growth by transposon mutagenesis in Saccharomyces cerevisiae." Moesch H.-U., Fink G.R. Genetics 145:671-684(1997) [PubMed] [Europe PMC] [Abstract] Cited for: GENE NAME, DISRUPTION PHENOTYPE.
[4]	"SRD5A3 is required for converting polyprenol to dolichol and is mutated in a congenital glycosylation disorder." Cantagrel V., Lefeber D.J., Ng B.G., Guan Z., Silhavy J.L., Bielas S.L., Lehle L., Hombauer H., Adamowicz M., Swiezewska E., De Brouwer A.P., Blumel P., Sykut-Cegielska J., Houliston S., Swistun D., Ali B.R., Dobyns W.B., Babovic-Vuksanovic D. , van Bokhoven H., Wevers R.A., Raetz C.R., Freeze H.H., Morava E., Al-Gazali L., Gleeson J.G. Cell 142:203-217(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z38060 Genomic DNA. Translation: CAA86173.1. BK006942 Genomic DNA. Translation: DAA08498.1.
PIR	S48430.
RefSeq	NP_012215.1. NM_001179399.1.
3D structure databases
ProteinModelPortal	P40526.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4658N.
MINT	MINT-512904.
STRING	4932.YIL049W.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL049W; YIL049W; YIL049W.
GeneID	854762.
KEGG	sce:YIL049W.
Organism-specific databases
CYGD	YIL049w.
SGD	S000001311. DFG10.
Phylogenomic databases
eggNOG	NOG330066.
HOGENOM	HOG000248191.
KO	K12345.
OMA	CPHYLDE.
OrthoDB	EOG4CJZSJ.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
Genevestigator	P40526.
GermOnline	YIL049W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001104. 3-oxo-5_a-steroid_4-DH_C. [Graphical view]
Pfam	PF02544. Steroid_dh. 1 hit. [Graphical view]
PROSITE	PS50244. S5A_REDUCTASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977508.

Entry information

Entry name

DFG10_YEAST

Accession

Primary (citable) accession number: P40526
Secondary accession number(s): D6VVN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program