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Protein

Polyprenol reductase

Gene

DFG10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism.1 Publication

Catalytic activityi

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: SGD
  • polyprenol reductase activity Source: UniProtKB-EC

GO - Biological processi

  • dolichol biosynthetic process Source: SGD
  • dolichol-linked oligosaccharide biosynthetic process Source: UniProtKB
  • dolichol metabolic process Source: UniProtKB
  • polyprenol catabolic process Source: UniProtKB
  • pseudohyphal growth Source: SGD

Keywordsi

Molecular functionOxidoreductase
LigandNADP

Enzyme and pathway databases

BioCyciMetaCyc:G3O-31320-MONOMER
YEAST:G3O-31320-MONOMER
UniPathwayiUPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
Polyprenol reductase (EC:1.3.1.94)
Alternative name(s):
Protein DFG10
Gene namesi
Name:DFG10
Ordered Locus Names:YIL049W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL049W
SGDiS000001311 DFG10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei18 – 38HelicalSequence analysisAdd BLAST21
Transmembranei78 – 98HelicalSequence analysisAdd BLAST21
Transmembranei123 – 143HelicalSequence analysisAdd BLAST21
Transmembranei200 – 220HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Suppression of filamentatous growth, defects in cell polarity, and cellular elongation, due to defects in N-glycosylation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002136821 – 253Polyprenol reductaseAdd BLAST253

Proteomic databases

MaxQBiP40526
PaxDbiP40526
PRIDEiP40526

Interactioni

Protein-protein interaction databases

BioGridi34941, 112 interactors
DIPiDIP-4658N
STRINGi4932.YIL049W

Structurei

3D structure databases

ProteinModelPortaliP40526
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000248191
InParanoidiP40526
KOiK12345
OMAiWILDICL
OrthoDBiEOG092C4J48

Family and domain databases

InterProiView protein in InterPro
IPR001104 3-oxo-5_a-steroid_4-DH_C
PfamiView protein in Pfam
PF02544 Steroid_dh, 1 hit
PROSITEiView protein in PROSITE
PS50244 S5A_REDUCTASE, 1 hit

Sequencei

Sequence statusi: Complete.

P40526-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYFDEEQLLK YTIYAYRLSF FVGICSLFIA KSCLPEFLQY GKTYRPKENS
60 70 80 90 100
KYSSILERIK KFTVPKAYFS HFYYLATFLS LVTLYFYPKF PIVWIIFGHS
110 120 130 140 150
LRRLYETLYV LHYTSNSRMN WSHYLVGIWF YSVLLLILNI SLYKNSIPNT
160 170 180 190 200
LNMNAFIIFC IASWDQYKNH VILANLVKYS LPTGRLFRLV CCPHYLDEII
210 220 230 240 250
IYSTLLPYEQ EFYLTLVWVI TSLTISALET KNYYRHKFKD NHVAPYAIIP

FII
Length:253
Mass (Da):30,306
Last modified:February 1, 1995 - v1
Checksum:iFBADD31A3BC5B054
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38060 Genomic DNA Translation: CAA86173.1
BK006942 Genomic DNA Translation: DAA08498.1
PIRiS48430
RefSeqiNP_012215.1, NM_001179399.1

Genome annotation databases

EnsemblFungiiYIL049W; YIL049W; YIL049W
GeneIDi854762
KEGGisce:YIL049W

Similar proteinsi

Entry informationi

Entry nameiDFG10_YEAST
AccessioniPrimary (citable) accession number: P40526
Secondary accession number(s): D6VVN2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 25, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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