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P40526

- DFG10_YEAST

UniProt

P40526 - DFG10_YEAST

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Protein

Polyprenol reductase

Gene
DFG10, YIL049W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism.1 Publication

Catalytic activityi

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.1 Publication

Pathwayi

GO - Molecular functioni

  1. 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: SGD

GO - Biological processi

  1. dolichol biosynthetic process Source: SGD
  2. dolichol-linked oligosaccharide biosynthetic process Source: UniProtKB
  3. dolichol metabolic process Source: UniProtKB
  4. polyprenol catabolic process Source: UniProtKB
  5. protein glycosylation Source: UniProtKB-UniPathway
  6. pseudohyphal growth Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciYEAST:G3O-31320-MONOMER.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyprenol reductase (EC:1.3.1.94)
Alternative name(s):
Protein DFG10
Gene namesi
Name:DFG10
Ordered Locus Names:YIL049W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIL049w.
SGDiS000001311. DFG10.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei18 – 3821Helical; Reviewed predictionAdd
BLAST
Transmembranei78 – 9821Helical; Reviewed predictionAdd
BLAST
Transmembranei123 – 14321Helical; Reviewed predictionAdd
BLAST
Transmembranei200 – 22021Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Suppression of filamentatous growth, defects in cell polarity, and cellular elongation, due to defects in N-glycosylation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 253253Polyprenol reductasePRO_0000213682Add
BLAST

Proteomic databases

MaxQBiP40526.

Expressioni

Gene expression databases

GenevestigatoriP40526.

Interactioni

Protein-protein interaction databases

BioGridi34941. 13 interactions.
DIPiDIP-4658N.
MINTiMINT-512904.
STRINGi4932.YIL049W.

Structurei

3D structure databases

ProteinModelPortaliP40526.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG330066.
HOGENOMiHOG000248191.
KOiK12345.
OMAiLVGIWFY.
OrthoDBiEOG7H1JX2.

Family and domain databases

InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40526-1 [UniParc]FASTAAdd to Basket

« Hide

MYFDEEQLLK YTIYAYRLSF FVGICSLFIA KSCLPEFLQY GKTYRPKENS    50
KYSSILERIK KFTVPKAYFS HFYYLATFLS LVTLYFYPKF PIVWIIFGHS 100
LRRLYETLYV LHYTSNSRMN WSHYLVGIWF YSVLLLILNI SLYKNSIPNT 150
LNMNAFIIFC IASWDQYKNH VILANLVKYS LPTGRLFRLV CCPHYLDEII 200
IYSTLLPYEQ EFYLTLVWVI TSLTISALET KNYYRHKFKD NHVAPYAIIP 250
FII 253
Length:253
Mass (Da):30,306
Last modified:February 1, 1995 - v1
Checksum:iFBADD31A3BC5B054
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z38060 Genomic DNA. Translation: CAA86173.1.
BK006942 Genomic DNA. Translation: DAA08498.1.
PIRiS48430.
RefSeqiNP_012215.1. NM_001179399.1.

Genome annotation databases

EnsemblFungiiYIL049W; YIL049W; YIL049W.
GeneIDi854762.
KEGGisce:YIL049W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z38060 Genomic DNA. Translation: CAA86173.1 .
BK006942 Genomic DNA. Translation: DAA08498.1 .
PIRi S48430.
RefSeqi NP_012215.1. NM_001179399.1.

3D structure databases

ProteinModelPortali P40526.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34941. 13 interactions.
DIPi DIP-4658N.
MINTi MINT-512904.
STRINGi 4932.YIL049W.

Proteomic databases

MaxQBi P40526.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIL049W ; YIL049W ; YIL049W .
GeneIDi 854762.
KEGGi sce:YIL049W.

Organism-specific databases

CYGDi YIL049w.
SGDi S000001311. DFG10.

Phylogenomic databases

eggNOGi NOG330066.
HOGENOMi HOG000248191.
KOi K12345.
OMAi LVGIWFY.
OrthoDBi EOG7H1JX2.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BioCyci YEAST:G3O-31320-MONOMER.

Miscellaneous databases

NextBioi 977508.
PROi P40526.

Gene expression databases

Genevestigatori P40526.

Family and domain databases

InterProi IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view ]
Pfami PF02544. Steroid_dh. 1 hit.
[Graphical view ]
PROSITEi PS50244. S5A_REDUCTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Dissection of filamentous growth by transposon mutagenesis in Saccharomyces cerevisiae."
    Moesch H.-U., Fink G.R.
    Genetics 145:671-684(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME, DISRUPTION PHENOTYPE.
  4. Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiDFG10_YEAST
AccessioniPrimary (citable) accession number: P40526
Secondary accession number(s): D6VVN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 14, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

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