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P40526 (DFG10_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polyprenol reductase

EC=1.3.1.94
Alternative name(s):
Protein DFG10
Gene names
Name:DFG10
Ordered Locus Names:YIL049W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length253 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Ref.4

Catalytic activity

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH. Ref.4

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Disruption phenotype

Suppression of filamentatous growth, defects in cell polarity, and cellular elongation, due to defects in N-glycosylation. Ref.3 Ref.4

Sequence similarities

Belongs to the steroid 5-alpha reductase family. Polyprenol reductase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 253253Polyprenol reductase
PRO_0000213682

Regions

Transmembrane18 – 3821Helical; Potential
Transmembrane78 – 9821Helical; Potential
Transmembrane123 – 14321Helical; Potential
Transmembrane200 – 22021Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
P40526 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: FBADD31A3BC5B054

FASTA25330,306
        10         20         30         40         50         60 
MYFDEEQLLK YTIYAYRLSF FVGICSLFIA KSCLPEFLQY GKTYRPKENS KYSSILERIK 

        70         80         90        100        110        120 
KFTVPKAYFS HFYYLATFLS LVTLYFYPKF PIVWIIFGHS LRRLYETLYV LHYTSNSRMN 

       130        140        150        160        170        180 
WSHYLVGIWF YSVLLLILNI SLYKNSIPNT LNMNAFIIFC IASWDQYKNH VILANLVKYS 

       190        200        210        220        230        240 
LPTGRLFRLV CCPHYLDEII IYSTLLPYEQ EFYLTLVWVI TSLTISALET KNYYRHKFKD 

       250 
NHVAPYAIIP FII 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Dissection of filamentous growth by transposon mutagenesis in Saccharomyces cerevisiae."
Moesch H.-U., Fink G.R.
Genetics 145:671-684(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE NAME, DISRUPTION PHENOTYPE.
[4]"SRD5A3 is required for converting polyprenol to dolichol and is mutated in a congenital glycosylation disorder."
Cantagrel V., Lefeber D.J., Ng B.G., Guan Z., Silhavy J.L., Bielas S.L., Lehle L., Hombauer H., Adamowicz M., Swiezewska E., De Brouwer A.P., Blumel P., Sykut-Cegielska J., Houliston S., Swistun D., Ali B.R., Dobyns W.B., Babovic-Vuksanovic D. expand/collapse author list , van Bokhoven H., Wevers R.A., Raetz C.R., Freeze H.H., Morava E., Al-Gazali L., Gleeson J.G.
Cell 142:203-217(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z38060 Genomic DNA. Translation: CAA86173.1.
BK006942 Genomic DNA. Translation: DAA08498.1.
PIRS48430.
RefSeqNP_012215.1. NM_001179399.1.

3D structure databases

ProteinModelPortalP40526.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34941. 13 interactions.
DIPDIP-4658N.
MINTMINT-512904.
STRING4932.YIL049W.

Proteomic databases

MaxQBP40526.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIL049W; YIL049W; YIL049W.
GeneID854762.
KEGGsce:YIL049W.

Organism-specific databases

CYGDYIL049w.
SGDS000001311. DFG10.

Phylogenomic databases

eggNOGNOG330066.
HOGENOMHOG000248191.
KOK12345.
OMALVGIWFY.
OrthoDBEOG7H1JX2.

Enzyme and pathway databases

BioCycYEAST:G3O-31320-MONOMER.
UniPathwayUPA00378.

Gene expression databases

GenevestigatorP40526.

Family and domain databases

InterProIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977508.
PROP40526.

Entry information

Entry nameDFG10_YEAST
AccessionPrimary (citable) accession number: P40526
Secondary accession number(s): D6VVN2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 14, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways