P40517 (YRB2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ran-specific GTPase-activating protein 2

Alternative name(s):
Ran-binding protein 2
Short name=RANBP2

Gene names

Name:	YRB2
Ordered Locus Names:	YIL063C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Important for the export of protein containing nuclear export signal (NES) out of the nucleus. Stimulates the GTPase activity of GSP1. Ref.4
Subunit structure	Interacts with GSP1, XPO1 and SRM1. Ref.4
Subcellular location	Nucleus Ref.4.
Domain	Contains X-F-X-F-G repeats.
Miscellaneous	Present with 3620 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 RanBD1 domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Nucleus
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of GTPase activity Inferred from electronic annotation. Source: GOC protein export from nucleus Inferred from mutant phenotype Ref.5. Source: SGD regulation of chromatin silencing at telomere Inferred from mutant phenotype PubMed 17904525. Source: SGD ribosomal small subunit export from nucleus Inferred from mutant phenotype PubMed 12082158. Source: SGD
Cellular_component	nuclear pore Inferred from direct assay PubMed 10684247. Source: SGD
Molecular_function	GTPase activator activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CRM1	P30822	1	EBI-29534,EBI-20589
YDR131C	Q03899	1	EBI-29534,EBI-36201
YHR080C	P38800	1	EBI-29534,EBI-24597

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Ran-specific GTPase-activating protein 2		PRO_0000213671
Regions
	Domain	191 – 327	137	RanBD1
Amino acid modifications
	Modified residue	14	1	Phosphoserine Ref.7 Ref.8
	Modified residue	31	1	Phosphothreonine Ref.7 Ref.8
	Modified residue	125	1	Phosphoserine Ref.8
	Modified residue	179	1	Phosphoserine Ref.7 Ref.8
	Modified residue	181	1	Phosphoserine Ref.7 Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P40517 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 7A1A626B9171646D	FASTA	327	36,054
	10 20 30 40 50 60 MSETNGGNAA RENSEVKQTA VENPIDKLDG TPKRPREKDQ DEQAEETSDK SEAPNKNDEE 70 80 90 100 110 120 KKEEGKKDQE PSHKKIKVDD GKTVESGIVE DDKKEDKFVF GAASKFGTGF GVAKKDTKDG 130 140 150 160 170 180 DATTSTESLP ASDSKTKKPF AFGSGLSFGS GFNILKNKTE NNSESEKKAT DVDKDKVHSG 190 200 210 220 230 240 SEQLANASED TKDKPKPLKL QKQEVKSGEE SEECIYQVNA KLYQLSNIKE GWKERGVGII 250 260 270 280 290 300 KINKSKDDVE KTRIVMRSRG ILKVILNIQL VKGFTVQKGF TGSLQSEKFI RLLAVDDNGD 310 320 PAQYAIKTGK KETTDELYNI IVKSVPK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Yrb2p is a nuclear protein that interacts with Prp20p, a yeast Rcc1 homologue." Taura T., Schlenstedt G., Silver P.A. J. Biol. Chem. 272:31877-31884(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SRM1.
[5]	"A member of the Ran-binding protein family, Yrb2p, is involved in nuclear protein export." Taura T., Krebber H., Silver P.A. Proc. Natl. Acad. Sci. U.S.A. 95:7427-7432(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14; THR-31; SER-179 AND SER-181, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14; THR-31; SER-125; SER-179 AND SER-181, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z38060 Genomic DNA. Translation: CAA86160.1. AY557879 Genomic DNA. Translation: AAS56205.1. BK006942 Genomic DNA. Translation: DAA08487.1.
PIR	S48416.
RefSeq	NP_012201.1. NM_001179413.1.
3D structure databases
ProteinModelPortal	P40517.
SMR	P40517. Positions 202-307.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2243N.
IntAct	P40517. 4 interactions.
MINT	MINT-486244.
STRING	4932.YIL063C.
Proteomic databases
PaxDb	P40517.
PeptideAtlas	P40517.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL063C; YIL063C; YIL063C.
GeneID	854747.
KEGG	sce:YIL063C.
Organism-specific databases
CYGD	YIL063c.
SGD	S000001325. YRB2.
Phylogenomic databases
eggNOG	NOG304969.
GeneTree	ENSGT00530000063644.
HOGENOM	HOG000141982.
KO	K15304.
OMA	GEESEEC.
OrthoDB	EOG40VZZ8.
Gene expression databases
Genevestigator	P40517.
GermOnline	YIL063C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.30.29.30. 1 hit.
InterPro	IPR011993. PH_like_dom. IPR000156. Ran_bind_dom. [Graphical view]
Pfam	PF00638. Ran_BP1. 1 hit. [Graphical view]
SMART	SM00160. RanBD. 1 hit. [Graphical view]
PROSITE	PS50196. RANBD1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977473.

Entry information

Entry name

YRB2_YEAST

Accession

Primary (citable) accession number: P40517
Secondary accession number(s): D6VVM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program