Reviewed, UniProtKB/Swiss-Prot P40510 (SER33_YEAST)

Names and origin

Protein names

Recommended name:
    D-3-phosphoglycerate dehydrogenase 2
      Short name=3-PGDH 2
    EC=1.1.1.95

Gene names

Name:	SER33
Ordered Locus Names:	YIL074C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH. 2-hydroxyglutarate + NAD+ = 2-oxoglutarate + NADH.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glyceric acid: step 1/3.
Miscellaneous	Present with 2010 molecules/cell in log phase SD medium. Ref.2
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Serine biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro amino acid binding Inferred from electronic annotation. Source: InterPro identical protein binding Inferred from physical interaction. Source: IntAct phosphoglycerate dehydrogenase activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-16821,EBI-16821
SER3	P40054	1	EBI-16821,EBI-16961

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	D-3-phosphoglycerate dehydrogenase 2		PRO_0000076019
Regions
	Domain	398 – 468	71	ACT
Sites
	Active site	287	1	By similarity
	Active site	316	1	By similarity
	Active site	347	1	Proton donor By similarity
Amino acid modifications
	Modified residue	22	1	Phosphoserine Ref.3 Ref.4
	Modified residue	28	1	Phosphothreonine Ref.4
	Modified residue	29	1	Phosphoserine Ref.4
	Modified residue	31	1	Phosphothreonine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P40510-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 23C59BAEA9FEDB36	FASTA	469	51,188
	10 20 30 40 50 60 MSYSAADNLQ DSFQRAMNFS GSPGAVSTSP TQSFMNTLPR RVSITKQPKA LKPFSTGDMN 70 80 90 100 110 120 ILLLENVNAT AIKIFKDQGY QVEFHKSSLP EDELIEKIKD VHAIGIRSKT RLTEKILQHA 130 140 150 160 170 180 RNLVCIGCFC IGTNQVDLKY AASKGIAVFN SPFSNSRSVA ELVIGEIISL ARQLGDRSIE 190 200 210 220 230 240 LHTGTWNKVA ARCWEVRGKT LGIIGYGHIG SQLSVLAEAM GLHVLYYDIV TIMALGTARQ 250 260 270 280 290 300 VSTLDELLNK SDFVTLHVPA TPETEKMLSA PQFAAMKDGA YVINASRGTV VDIPSLIQAV 310 320 330 340 350 360 KANKIAGAAL DVYPHEPAKN GEGSFNDELN SWTSELVSLP NIILTPHIGG STEEAQSSIG 370 380 390 400 410 420 IEVATALSKY INEGNSVGSV NFPEVSLKSL DYDQENTVRV LYIHRNVPGV LKTVNDILSD 430 440 450 460 HNIEKQFSDS HGEIAYLMAD ISSVNQSEIK DIYEKLNQTS AKVSIRLLY

References

[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed: 9169870] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[3]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22, MASS SPECTROMETRY.
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22; THR-28; SER-29 AND THR-31, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z37997 Genomic DNA. Translation: CAA86096.1.
PIR	S48370.
RefSeq	NP_012191.1.
3D structure databases
HSSP	HSSP built from PDB template 1PSD based on UniProtKB P08328.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4483N.
IntAct	P40510. 8 interactions.
Proteomic databases
PeptideAtlas	P40510.
PRIDE	P40510.
Genome annotation databases
Ensembl	YIL074C. Saccharomyces cerevisiae. [Contig view]
GeneID	854736.
GenomeReviews	Gene locus YIL074C in contig Z47047_GR.
KEGG	sce:YIL074C.
NMPDR	fig|4932.3.peg.1718.
Organism-specific databases
CYGD	YIL074c.
SGD	S000001336. SER33.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P40510.
OMA	P40510. ANDGISK.
Enzyme and pathway databases
BRENDA	1.1.1.95. 250.
Gene expression databases
GermOnline	YIL074C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. PF01842. ACT. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	977440.

Entry information

Entry name

SER33_YEAST

Accession

Primary (citable) accession number: P40510

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)