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P40509

- COPE_YEAST

UniProt

P40509 - COPE_YEAST

Protein

Coatomer subunit epsilon

Gene

SEC28

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (15 Dec 1998)
      Previous versions | rss
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    Functioni

    The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. structural molecule activity Source: InterPro

    GO - Biological processi

    1. ER to Golgi vesicle-mediated transport Source: SGD
    2. late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
    3. protein transport Source: UniProtKB-KW
    4. retrograde vesicle-mediated transport, Golgi to ER Source: InterPro
    5. vesicle coating Source: SGD

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31341-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Coatomer subunit epsilon
    Alternative name(s):
    Epsilon-coat protein
    Short name:
    Epsilon-COP
    Gene namesi
    Name:SEC28
    Ordered Locus Names:YIL076W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL076w.
    SGDiS000001338. SEC28.

    Subcellular locationi

    Cytoplasm By similarity. Golgi apparatus membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cytoplasmic vesicleCOPI-coated vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
    Note: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.By similarity

    GO - Cellular componenti

    1. COPI vesicle coat Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 296296Coatomer subunit epsilonPRO_0000193856Add
    BLAST

    Proteomic databases

    MaxQBiP40509.
    PaxDbiP40509.
    PeptideAtlasiP40509.

    Expressioni

    Gene expression databases

    GenevestigatoriP40509.

    Interactioni

    Subunit structurei

    Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Interacts with the ESCRT-0 subunit VPS27.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    COP1P536225EBI-4884,EBI-4860
    SEC27P418114EBI-4884,EBI-4898
    VPS27P403432EBI-4884,EBI-20380

    Protein-protein interaction databases

    BioGridi34916. 340 interactions.
    DIPiDIP-4711N.
    IntActiP40509. 18 interactions.
    MINTiMINT-429031.
    STRINGi4932.YIL076W.

    Structurei

    Secondary structure

    1
    296
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 107
    Turni11 – 133
    Helixi15 – 184
    Helixi20 – 234
    Helixi31 – 4313
    Beta strandi52 – 543
    Helixi57 – 6711
    Helixi73 – 775
    Turni78 – 814
    Helixi86 – 9914
    Helixi102 – 11312
    Turni114 – 1174
    Beta strandi118 – 1203
    Helixi121 – 13414
    Helixi138 – 15114
    Helixi154 – 17522
    Turni179 – 1813
    Helixi182 – 19110
    Helixi197 – 21014
    Helixi213 – 22412
    Helixi226 – 2294
    Turni230 – 2323
    Helixi234 – 2363
    Beta strandi237 – 2393
    Helixi240 – 25314
    Helixi259 – 2679
    Helixi273 – 29018

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3MV2X-ray2.90B/D/F1-296[»]
    3MV3X-ray3.25B/D/F1-296[»]
    ProteinModelPortaliP40509.
    SMRiP40509. Positions 1-293.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP40509.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the COPE family.Curated

    Phylogenomic databases

    eggNOGiNOG252891.
    HOGENOMiHOG000000967.
    KOiK17268.
    OMAiASTMFEN.
    OrthoDBiEOG7PCJT7.

    Family and domain databases

    InterProiIPR006822. Coatomer_esu.
    [Graphical view]
    PANTHERiPTHR10805. PTHR10805. 1 hit.
    PfamiPF04733. Coatomer_E. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016478. Coatomer_esu. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P40509-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYFNIKQNY YTGNFVQCLQ EIEKFSKVTD NTLLFYKAKT LLALGQYQSQ    50
    DPTSKLGKVL DLYVQFLDTK NIEELENLLK DKQNSPYELY LLATAQAILG 100
    DLDKSLETCV EGIDNDEAEG TTELLLLAIE VALLNNNVST ASTIFDNYTN 150
    AIEDTVSGDN EMILNLAESY IKFATNKETA TSNFYYYEEL SQTFPTWKTQ 200
    LGLLNLHLQQ RNIAEAQGIV ELLLSDYYSV EQKENAVLYK PTFLANQITL 250
    ALMQGLDTED LTNQLVKLDH EHAFIKHHQE IDAKFDELVR KYDTSN 296
    Length:296
    Mass (Da):33,829
    Last modified:December 15, 1998 - v2
    Checksum:i95ACA8BB647817DB
    GO

    Sequence cautioni

    The sequence CAA86094.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF043582 Genomic DNA. Translation: AAC14023.1.
    Z37997 Genomic DNA. Translation: CAA86094.1. Different initiation.
    BK006942 Genomic DNA. Translation: DAA08474.1.
    PIRiS48368.
    RefSeqiNP_012189.2. NM_001179426.1.

    Genome annotation databases

    EnsemblFungiiYIL076W; YIL076W; YIL076W.
    GeneIDi854734.
    KEGGisce:YIL076W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF043582 Genomic DNA. Translation: AAC14023.1 .
    Z37997 Genomic DNA. Translation: CAA86094.1 . Different initiation.
    BK006942 Genomic DNA. Translation: DAA08474.1 .
    PIRi S48368.
    RefSeqi NP_012189.2. NM_001179426.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3MV2 X-ray 2.90 B/D/F 1-296 [» ]
    3MV3 X-ray 3.25 B/D/F 1-296 [» ]
    ProteinModelPortali P40509.
    SMRi P40509. Positions 1-293.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34916. 340 interactions.
    DIPi DIP-4711N.
    IntActi P40509. 18 interactions.
    MINTi MINT-429031.
    STRINGi 4932.YIL076W.

    Proteomic databases

    MaxQBi P40509.
    PaxDbi P40509.
    PeptideAtlasi P40509.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL076W ; YIL076W ; YIL076W .
    GeneIDi 854734.
    KEGGi sce:YIL076W.

    Organism-specific databases

    CYGDi YIL076w.
    SGDi S000001338. SEC28.

    Phylogenomic databases

    eggNOGi NOG252891.
    HOGENOMi HOG000000967.
    KOi K17268.
    OMAi ASTMFEN.
    OrthoDBi EOG7PCJT7.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31341-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P40509.
    NextBioi 977434.

    Gene expression databases

    Genevestigatori P40509.

    Family and domain databases

    InterProi IPR006822. Coatomer_esu.
    [Graphical view ]
    PANTHERi PTHR10805. PTHR10805. 1 hit.
    Pfami PF04733. Coatomer_E. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016478. Coatomer_esu. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Epsilon-COP is a structural component of coatomer that functions to stabilize alpha-COP."
      Duden R., Kajikawa L., Wuestehube L., Schekman R.
      EMBO J. 17:985-995(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION OF INITIATION SITE, FUNCTION.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE."
      Zhang Z., Dietrich F.S.
      Nucleic Acids Res. 33:2838-2851(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
    7. "Involvement of specific COPI subunits in protein sorting from the late endosome to the vacuole in yeast."
      Gabriely G., Kama R., Gerst J.E.
      Mol. Cell. Biol. 27:526-540(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH VPS27.

    Entry informationi

    Entry nameiCOPE_YEAST
    AccessioniPrimary (citable) accession number: P40509
    Secondary accession number(s): D6VVK8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 23100 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3