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Protein

Coatomer subunit epsilon

Gene

SEC28

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.2 Publications

GO - Molecular functioni

  • promoter-specific chromatin binding Source: SGD
  • structural molecule activity Source: InterPro

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: SGD
  • intra-Golgi vesicle-mediated transport Source: GO_Central
  • late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
  • protein transport Source: UniProtKB-KW
  • retrograde vesicle-mediated transport, Golgi to ER Source: GO_Central
  • vesicle coating Source: SGD
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31341-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Coatomer subunit epsilon
Alternative name(s):
Epsilon-coat protein
Short name:
Epsilon-COP
Gene namesi
Name:SEC28
Ordered Locus Names:YIL076W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL076W.
SGDiS000001338. SEC28.

Subcellular locationi

GO - Cellular componenti

  • COPI vesicle coat Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001938561 – 296Coatomer subunit epsilonAdd BLAST296

Proteomic databases

MaxQBiP40509.
PRIDEiP40509.

Interactioni

Subunit structurei

Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Interacts with the ESCRT-0 subunit VPS27.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
COP1P536225EBI-4884,EBI-4860
SEC27P418114EBI-4884,EBI-4898
VPS27P403432EBI-4884,EBI-20380

Protein-protein interaction databases

BioGridi34916. 348 interactors.
DIPiDIP-4711N.
IntActiP40509. 18 interactors.
MINTiMINT-429031.

Structurei

Secondary structure

1296
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 10Combined sources7
Turni11 – 13Combined sources3
Helixi15 – 18Combined sources4
Helixi20 – 23Combined sources4
Helixi31 – 43Combined sources13
Beta strandi52 – 54Combined sources3
Helixi57 – 67Combined sources11
Helixi73 – 77Combined sources5
Turni78 – 81Combined sources4
Helixi86 – 99Combined sources14
Helixi102 – 113Combined sources12
Turni114 – 117Combined sources4
Beta strandi118 – 120Combined sources3
Helixi121 – 134Combined sources14
Helixi138 – 151Combined sources14
Helixi154 – 175Combined sources22
Turni179 – 181Combined sources3
Helixi182 – 191Combined sources10
Helixi197 – 210Combined sources14
Helixi213 – 224Combined sources12
Helixi226 – 229Combined sources4
Turni230 – 232Combined sources3
Helixi234 – 236Combined sources3
Beta strandi237 – 239Combined sources3
Helixi240 – 253Combined sources14
Helixi259 – 267Combined sources9
Helixi273 – 290Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MV2X-ray2.90B/D/F1-296[»]
3MV3X-ray3.25B/D/F1-296[»]
ProteinModelPortaliP40509.
SMRiP40509.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40509.

Family & Domainsi

Sequence similaritiesi

Belongs to the COPE family.Curated

Phylogenomic databases

HOGENOMiHOG000000967.
InParanoidiP40509.
KOiK17268.
OMAiQTFPTWK.
OrthoDBiEOG092C3TYJ.

Family and domain databases

InterProiIPR006822. Coatomer_esu.
[Graphical view]
PANTHERiPTHR10805. PTHR10805. 1 hit.
PIRSFiPIRSF016478. Coatomer_esu. 1 hit.

Sequencei

Sequence statusi: Complete.

P40509-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYFNIKQNY YTGNFVQCLQ EIEKFSKVTD NTLLFYKAKT LLALGQYQSQ
60 70 80 90 100
DPTSKLGKVL DLYVQFLDTK NIEELENLLK DKQNSPYELY LLATAQAILG
110 120 130 140 150
DLDKSLETCV EGIDNDEAEG TTELLLLAIE VALLNNNVST ASTIFDNYTN
160 170 180 190 200
AIEDTVSGDN EMILNLAESY IKFATNKETA TSNFYYYEEL SQTFPTWKTQ
210 220 230 240 250
LGLLNLHLQQ RNIAEAQGIV ELLLSDYYSV EQKENAVLYK PTFLANQITL
260 270 280 290
ALMQGLDTED LTNQLVKLDH EHAFIKHHQE IDAKFDELVR KYDTSN
Length:296
Mass (Da):33,829
Last modified:December 15, 1998 - v2
Checksum:i95ACA8BB647817DB
GO

Sequence cautioni

The sequence CAA86094 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043582 Genomic DNA. Translation: AAC14023.1.
Z37997 Genomic DNA. Translation: CAA86094.1. Different initiation.
BK006942 Genomic DNA. Translation: DAA08474.1.
PIRiS48368.
RefSeqiNP_012189.2. NM_001179426.1.

Genome annotation databases

EnsemblFungiiYIL076W; YIL076W; YIL076W.
GeneIDi854734.
KEGGisce:YIL076W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043582 Genomic DNA. Translation: AAC14023.1.
Z37997 Genomic DNA. Translation: CAA86094.1. Different initiation.
BK006942 Genomic DNA. Translation: DAA08474.1.
PIRiS48368.
RefSeqiNP_012189.2. NM_001179426.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MV2X-ray2.90B/D/F1-296[»]
3MV3X-ray3.25B/D/F1-296[»]
ProteinModelPortaliP40509.
SMRiP40509.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34916. 348 interactors.
DIPiDIP-4711N.
IntActiP40509. 18 interactors.
MINTiMINT-429031.

Proteomic databases

MaxQBiP40509.
PRIDEiP40509.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL076W; YIL076W; YIL076W.
GeneIDi854734.
KEGGisce:YIL076W.

Organism-specific databases

EuPathDBiFungiDB:YIL076W.
SGDiS000001338. SEC28.

Phylogenomic databases

HOGENOMiHOG000000967.
InParanoidiP40509.
KOiK17268.
OMAiQTFPTWK.
OrthoDBiEOG092C3TYJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-31341-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP40509.
PROiP40509.

Family and domain databases

InterProiIPR006822. Coatomer_esu.
[Graphical view]
PANTHERiPTHR10805. PTHR10805. 1 hit.
PIRSFiPIRSF016478. Coatomer_esu. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCOPE_YEAST
AccessioniPrimary (citable) accession number: P40509
Secondary accession number(s): D6VVK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 15, 1998
Last modified: November 2, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 23100 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.