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Protein

Phosphopantothenate--cysteine ligase CAB2

Gene

CAB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine.1 Publication

Catalytic activityi

CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-((R)-4'-phosphopantothenoyl)-L-cysteine.

Pathwayi: coenzyme A biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes CoA from (R)-pantothenate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pantothenate kinase CAB1 (CAB1)
  2. Phosphopantothenate--cysteine ligase CAB2 (CAB2)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Dephospho-CoA kinase CAB5 (CAB5)
This subpathway is part of the pathway coenzyme A biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CoA from (R)-pantothenate, the pathway coenzyme A biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • phosphopantothenate--cysteine ligase activity Source: SGD

GO - Biological processi

  • coenzyme A biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Coenzyme A biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-285.
YEAST:MONOMER3O-285.
ReactomeiR-SCE-196783. Coenzyme A biosynthesis.
UniPathwayiUPA00241; UER00353.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphopantothenate--cysteine ligase CAB2 (EC:6.3.2.5)
Alternative name(s):
Coenzyme A biosynthesis protein 2
Phosphopantothenoylcysteine synthetase
Short name:
PPC synthetase
Gene namesi
Name:CAB2
Ordered Locus Names:YIL083C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL083C.
SGDiS000001345. CAB2.

Subcellular locationi

GO - Cellular componenti

  • CoA-synthesizing protein complex Source: SGD
  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001820421 – 365Phosphopantothenate--cysteine ligase CAB2Add BLAST365

Proteomic databases

MaxQBiP40506.
PRIDEiP40506.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
CAB3P360766EBI-25089,EBI-26778
CAB4P533326EBI-25089,EBI-23648

Protein-protein interaction databases

BioGridi34909. 54 interactors.
IntActiP40506. 3 interactors.
MINTiMINT-4489840.

Structurei

3D structure databases

ProteinModelPortaliP40506.
SMRiP40506.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPC synthetase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000015263.
InParanoidiP40506.
KOiK01922.
OMAiPYERWIR.
OrthoDBiEOG092C41HT.

Family and domain databases

Gene3Di3.40.50.10300. 1 hit.
InterProiIPR007085. DNA/pantothenate-metab_flavo_C.
[Graphical view]
PfamiPF04127. DFP. 2 hits.
[Graphical view]
SUPFAMiSSF102645. SSF102645. 1 hit.

Sequencei

Sequence statusi: Complete.

P40506-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPLPVLNRP QIHTSVTEIS HAIDRTIKEE LFPVAYTTEE EQYFKTNPKP
60 70 80 90 100
AYIDELIKDA KEFIDLQYSL KRNKIVLITS GGTTVPLENN TVRFIDNFSA
110 120 130 140 150
GTRGASSAEQ FLANGYSVIF LHREFSLTPY NRSFSHSINT LFLDYIDSEG
160 170 180 190 200
KIKPEFAENV LKNKKLYDKY MEKEEKLLLL PFTTVNQYLW SLKSIAKLLN
210 220 230 240 250
NSGCLFYLAA AVSDFFVPYS RLPQHKIQSG DNGKMGANND TEGTTRTTPD
260 270 280 290 300
GKLIVNLDPV PKFLRRLVES WATQAMIVSF KLETDESMLL YKCTQALDRY
310 320 330 340 350
NHQLVIGNLL QTRNKQVIFV SPENRKGDWV RLDEKHHSIE EMIIPEVIAR
360
HDKWVAHSKT KLATK
Length:365
Mass (Da):41,867
Last modified:September 21, 2011 - v2
Checksum:iD67655BC0267DDAB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti338S → I in CAA86711 (PubMed:9169870).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46728 Genomic DNA. Translation: CAA86711.1.
BK006942 Genomic DNA. Translation: DAA08469.2.
PIRiS49797.
RefSeqiNP_012183.2. NM_001179431.2.

Genome annotation databases

EnsemblFungiiYIL083C; YIL083C; YIL083C.
GeneIDi854726.
KEGGisce:YIL083C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46728 Genomic DNA. Translation: CAA86711.1.
BK006942 Genomic DNA. Translation: DAA08469.2.
PIRiS49797.
RefSeqiNP_012183.2. NM_001179431.2.

3D structure databases

ProteinModelPortaliP40506.
SMRiP40506.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34909. 54 interactors.
IntActiP40506. 3 interactors.
MINTiMINT-4489840.

Proteomic databases

MaxQBiP40506.
PRIDEiP40506.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL083C; YIL083C; YIL083C.
GeneIDi854726.
KEGGisce:YIL083C.

Organism-specific databases

EuPathDBiFungiDB:YIL083C.
SGDiS000001345. CAB2.

Phylogenomic databases

GeneTreeiENSGT00390000015263.
InParanoidiP40506.
KOiK01922.
OMAiPYERWIR.
OrthoDBiEOG092C41HT.

Enzyme and pathway databases

UniPathwayiUPA00241; UER00353.
BioCyciMetaCyc:MONOMER3O-285.
YEAST:MONOMER3O-285.
ReactomeiR-SCE-196783. Coenzyme A biosynthesis.

Miscellaneous databases

PROiP40506.

Family and domain databases

Gene3Di3.40.50.10300. 1 hit.
InterProiIPR007085. DNA/pantothenate-metab_flavo_C.
[Graphical view]
PfamiPF04127. DFP. 2 hits.
[Graphical view]
SUPFAMiSSF102645. SSF102645. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPPCS_YEAST
AccessioniPrimary (citable) accession number: P40506
Secondary accession number(s): D6VVK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: September 21, 2011
Last modified: November 2, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3170 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.