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Protein

Phosphopantothenate--cysteine ligase CAB2

Gene

CAB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine.1 Publication

Miscellaneous

Present with 3170 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-((R)-4'-phosphopantothenoyl)-L-cysteine.

Pathwayi: coenzyme A biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes CoA from (R)-pantothenate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pantothenate kinase CAB1 (CAB1)
  2. Phosphopantothenate--cysteine ligase CAB2 (CAB2)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Dephospho-CoA kinase CAB5 (CAB5)
This subpathway is part of the pathway coenzyme A biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CoA from (R)-pantothenate, the pathway coenzyme A biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • phosphopantothenate--cysteine ligase activity Source: SGD

GO - Biological processi

  • coenzyme A biosynthetic process Source: SGD

Keywordsi

Molecular functionLigase
Biological processCoenzyme A biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-285
YEAST:MONOMER3O-285
ReactomeiR-SCE-196783 Coenzyme A biosynthesis
UniPathwayiUPA00241; UER00353

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphopantothenate--cysteine ligase CAB2 (EC:6.3.2.5)
Alternative name(s):
Coenzyme A biosynthesis protein 2
Phosphopantothenoylcysteine synthetase
Short name:
PPC synthetase
Gene namesi
Name:CAB2
Ordered Locus Names:YIL083C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL083C
SGDiS000001345 CAB2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001820421 – 365Phosphopantothenate--cysteine ligase CAB2Add BLAST365

Proteomic databases

MaxQBiP40506
PaxDbiP40506
PRIDEiP40506

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34909, 67 interactors
IntActiP40506, 3 interactors
STRINGi4932.YIL083C

Structurei

3D structure databases

ProteinModelPortaliP40506
SMRiP40506
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPC synthetase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000015263
InParanoidiP40506
KOiK01922
OMAiRSAFPYA
OrthoDBiEOG092C41HT

Family and domain databases

Gene3Di3.40.50.10300, 1 hit
InterProiView protein in InterPro
IPR035929 CoaB-like_sf
IPR007085 DNA/pantothenate-metab_flavo_C
PfamiView protein in Pfam
PF04127 DFP, 2 hits
SUPFAMiSSF102645 SSF102645, 1 hit

Sequencei

Sequence statusi: Complete.

P40506-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPLPVLNRP QIHTSVTEIS HAIDRTIKEE LFPVAYTTEE EQYFKTNPKP
60 70 80 90 100
AYIDELIKDA KEFIDLQYSL KRNKIVLITS GGTTVPLENN TVRFIDNFSA
110 120 130 140 150
GTRGASSAEQ FLANGYSVIF LHREFSLTPY NRSFSHSINT LFLDYIDSEG
160 170 180 190 200
KIKPEFAENV LKNKKLYDKY MEKEEKLLLL PFTTVNQYLW SLKSIAKLLN
210 220 230 240 250
NSGCLFYLAA AVSDFFVPYS RLPQHKIQSG DNGKMGANND TEGTTRTTPD
260 270 280 290 300
GKLIVNLDPV PKFLRRLVES WATQAMIVSF KLETDESMLL YKCTQALDRY
310 320 330 340 350
NHQLVIGNLL QTRNKQVIFV SPENRKGDWV RLDEKHHSIE EMIIPEVIAR
360
HDKWVAHSKT KLATK
Length:365
Mass (Da):41,867
Last modified:September 21, 2011 - v2
Checksum:iD67655BC0267DDAB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti338S → I in CAA86711 (PubMed:9169870).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46728 Genomic DNA Translation: CAA86711.1
BK006942 Genomic DNA Translation: DAA08469.2
PIRiS49797
RefSeqiNP_012183.2, NM_001179431.2

Genome annotation databases

EnsemblFungiiYIL083C; YIL083C; YIL083C
GeneIDi854726
KEGGisce:YIL083C

Similar proteinsi

Entry informationi

Entry nameiPPCS_YEAST
AccessioniPrimary (citable) accession number: P40506
Secondary accession number(s): D6VVK3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: September 21, 2011
Last modified: May 23, 2018
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

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