P40504 (KTR7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable mannosyltransferase KTR7
EC=2.4.1.-

Gene names

Name:	KTR7
Ordered Locus Names:	YIL085C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possible glycosyltransferase.
Catalytic activity	Transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->2)-D-mannosyl-D-mannose linkage.
Subcellular location	Membrane; Single-pass type II membrane protein Probable.
Miscellaneous	Present with 2890 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the glycosyltransferase 15 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fungal-type cell wall organization Inferred from mutant phenotype Ref.3. Source: SGD protein glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	mannosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 517	517	Probable mannosyltransferase KTR7		PRO_0000208248
Regions
	Topological domain	1 – 23	23	Cytoplasmic Potential
	Transmembrane	24 – 44	21	Helical; Signal-anchor for type II membrane protein
	Topological domain	45 – 517	473	Lumenal Potential
	Region	45 – 85	41	Stem region By similarity
	Region	86 – 517	432	Catalytic By similarity
Sites
	Active site	367	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
	Glycosylation	144	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P40504 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 3E02FA8AA5456DEF	FASTA	517	61,438
	10 20 30 40 50 60 MAIRLNPKVR RFLLDKCRQK RYGFLFLGCI FAILYCMGTW PFFAKDIVHD PNNLPYSLQD 70 80 90 100 110 120 YSTDKDEPFF RGCTDTKLYL QNPAYSKMNA SFVMLTRNEE IEDVLKTMRS IEGHFNKWFK 130 140 150 160 170 180 YPYVFLNDDP FTDHFKDQIQ AATNATVEFG TVDEIMWEFP AKVRNSLQFK ASLEDQNDRG 190 200 210 220 230 240 IMYGNMESYH KMCRFYSGIF YKHPLVSKYE WYWRIEPDVD FFCDISYDPF FEMAKHNKKY 250 260 270 280 290 300 GFTVLITELY WTVPNLFRTT KSFIKKTAGL KENLGTLWKL FTFNYNILDT DDEEISRWVN 310 320 330 340 350 360 FPWDAKPKLT EKLMVDFLLE NHGQVNNEED LEGIQYLVER ARSKVPMLED SLEGEDYNLC 370 380 390 400 410 420 HFWSNFEIAR VDLFDNEIYN AYFKFLEESG GFWTERWGDA PIHSIGLGMT LDLEDVHYFR 430 440 450 460 470 480 DIGYRHSSLQ HCPKNALQSQ ENLNTFDEGY NFGCGCRCVC PKKGEDIEDH STPCMDIFFE 490 500 510 LLHGREYEKE FPGCYKPSIK DKDVIEEIRR ENFRVIE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed: 9169870] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Completion of the Saccharomyces cerevisiae genome sequence allows identification of KTR5, KTR6 and KTR7 and definition of the nine-membered KRE2/MNT1 mannosyltransferase gene family in this organism." Lussier M., Sdicu A.-M., Winnett E., Vo D.H., Sheraton J., Duesterhoeft A., Storms R.K., Bussey H. Yeast 13:267-274(1997) [PubMed: 9090056] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46728 Genomic DNA. Translation: CAA86709.1. BK006942 Genomic DNA. Translation: DAA08467.1.
PIR	S49795.
RefSeq	NP_012181.1. NM_001179433.1.
3D structure databases
ProteinModelPortal	P40504.
SMR	P40504. Positions 81-486.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4677N.
IntAct	P40504. 3 interactions.
MINT	MINT-536153.
STRING	P40504.
Protein family/group databases
CAZy	GT15. Glycosyltransferase Family 15.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL085C; YIL085C; YIL085C.
GeneID	854724.
KEGG	sce:YIL085C.
NMPDR	fig|4932.3.peg.1708.
Organism-specific databases
CYGD	YIL085c.
SGD	S000001347. KTR7.
Phylogenomic databases
eggNOG	fuNOG05672.
GeneTree	EFGT00050000003570.
HOGENOM	HBG396033.
OMA	CINEWAS.
OrthoDB	EOG4F4WKD.
Gene expression databases
ArrayExpress	P40504.
Genevestigator	P40504.
GermOnline	YIL085C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002685. Glyco_trans_15. [Graphical view]
KO	K03854.
Pfam	PF01793. Glyco_transf_15. 1 hit. [Graphical view]
PIRSF	PIRSF018153. Glyco_trans_15. 1 hit.
ProtoNet	Search...
Other
NextBio	977409.

Entry information

Entry name

KTR7_YEAST

Accession

Primary (citable) accession number: P40504
Secondary accession number(s): D6VVK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program