P40498 (UTP25_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
U3 small nucleolar RNA-associated protein 25
Short name=U3 snoRNA-associated protein 25

Alternative name(s):
U three protein 25

Gene names

Name:	UTP25
Ordered Locus Names:	YIL091C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	721 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DEAD-box RNA helicase-like protein required for pre-18S rRNA processing, specifically at sites A0, A1, and A2. Ref.7 Ref.8
Subunit structure	Interacts with snoRNA U3. Interacts with MPP10, NOP19, RRP9, UTP8 and UTP18. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Ref.7 Ref.8
Subcellular location	Nucleus › nucleolus Ref.3.
Miscellaneous	Present with 172 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the UTP25 family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Molecular function	Ribonucleoprotein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype PubMed 20528918Ref.7. Source: SGD
Cellular_component	nucleolus Inferred from direct assay Ref.3PubMed 14690591PubMed 20528918. Source: SGD small-subunit processome Inferred from direct assay Ref.7. Source: SGD
Molecular_function	U3 snoRNA binding Inferred from direct assay PubMed 20528918. Source: SGD rRNA binding Inferred from direct assay PubMed 20528918. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 721	721	U3 small nucleolar RNA-associated protein 25		PRO_0000202974
Amino acid modifications
	Modified residue	196	1	Phosphoserine Ref.5 Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P40498 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 301BC87BCA027104	FASTA	721	83,991
	10 20 30 40 50 60 MSDSSVREKN DNFRGYRKRG RQELRKIKRS SARTEGGSTE TLEDVAEDID HRSDEDEVSD 70 80 90 100 110 120 VDSGDDFDIE DEEGKKEKVY DALLTILKSE HPEPKRRRRE ADESNKAPAE VGEDEHENTE 130 140 150 160 170 180 HGPVDDQLEI ENGLLGNHED DNDDDSSGDE KDIDSEDEQD PFESHFNQVP EKFVDKLSNA 190 200 210 220 230 240 FKTKSVKYKS VKGSLSDSES YIYAKPVVIG EEALVESPYR SSSIYSYFLK QRLKVQNGLL 250 260 270 280 290 300 DKKTDPLTAL QKKLVDPMFQ YKDILYEYDS YEKDEDEYRD LYALHVLNHI YKTRDRILKN 310 320 330 340 350 360 NQRLQDNPDT EHLDQGFTRP KVLIVVPTRE VAYRVVDKII SKSGIDQVDK KGKFYDQFRD 370 380 390 400 410 420 DSLPPKSKPK SFQHIFRGNT NDFFVVGLKF TRKAIKLYSN FYQSDIIVCS PLGIQMILEN 430 440 450 460 470 480 TDKKKRQDDF LSSIELMVID QLHSIEYQNI SHIFTIFDHL NKIPDQQHEA DFSRIRMWYI 490 500 510 520 530 540 NEQAKLFRQT MVFTKYISPA ANSLINGRCR NMAGRWKNHK VIGSENSSIG QSGLKIRQIF 550 560 570 580 590 600 QRFDIIGNSI IEEPDYRFKF FTSVIIPGIV KSTGYEDGIL IYIPDYTDFI RIRNYMKEKT 610 620 630 640 650 660 TILFGDINEY SSQRQLNANR SLFQQGRLKV MLYTERLHHY RRYEIKGVKS VVFYKPPNNP 670 680 690 700 710 720 EFYNEVVRFI GKNAFLGNTD LNISTVRCIY SKLDGLSLER IVGTKRAAVL SHAQKEIYEF K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-196, MASS SPECTROMETRY.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-196, MASS SPECTROMETRY.
[7]	"The DEAD-box RNA helicase-like Utp25 is an SSU processome component." Charette J.M., Baserga S.J. RNA 16:2156-2169(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE SSU PROCESSOME, FUNCTION, INTERACTION WITH U3 SNORNA; MMP10; RRP9; UTP8 AND UTP18.
[8]	"The nucleolar protein Nop19p interacts preferentially with Utp25p and Dhr2p and is essential for the production of the 40S ribosomal subunit in Saccharomyces cerevisiae." Choque E., Marcellin M., Burlet-Schiltz O., Gadal O., Dez C. RNA Biol. 8:1158-1172(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NOP19, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46728 Genomic DNA. Translation: CAA86703.1. BK006942 Genomic DNA. Translation: DAA08462.1.
PIR	S49789.
RefSeq	NP_012175.1. NM_001179439.1.
3D structure databases
ProteinModelPortal	P40498.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5629N.
IntAct	P40498. 3 interactions.
MINT	MINT-540194.
STRING	4932.YIL091C.
Proteomic databases
PaxDb	P40498.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL091C; YIL091C; YIL091C.
GeneID	854717.
KEGG	sce:YIL091C.
Organism-specific databases
CYGD	YIL091c.
SGD	S000001353. UTP25.
Phylogenomic databases
eggNOG	NOG238048.
GeneTree	ENSGT00390000000709.
KO	K14774.
OMA	WYINEQA.
OrthoDB	EOG49PF7K.
Gene expression databases
Genevestigator	P40498.
GermOnline	YIL091C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR010678. Digest_organ_expansion_fac-prd. [Graphical view]
PANTHER	PTHR12933. PTHR12933. 1 hit.
Pfam	PF06862. DUF1253. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977392.

Entry information

Entry name

UTP25_YEAST

Accession

Primary (citable) accession number: P40498
Secondary accession number(s): D6VVJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program