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Protein

Actin-regulating kinase PRK1

Gene

PRK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in regulation of actin cytoskeleton organization and endocytosis.3 Publications

Miscellaneous

Present with 1323 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei56ATPPROSITE-ProRule annotation1
Active sitei158Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi28 – 36ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  • actin cortical patch assembly Source: SGD
  • actin filament organization Source: SGD
  • protein phosphorylation Source: SGD
  • regulation of endocytosis Source: SGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31354-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-regulating kinase PRK1 (EC:2.7.11.1)
Alternative name(s):
p53-regulating kinase 1
Gene namesi
Name:PRK1
Synonyms:PAK1
Ordered Locus Names:YIL095W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL095W
SGDiS000001357 PRK1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi56K → A: Abolishes protein kinase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000865811 – 810Actin-regulating kinase PRK1Add BLAST810

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei402PhosphoserineCombined sources1
Modified residuei428PhosphoserineCombined sources1
Modified residuei484PhosphoserineCombined sources1
Modified residuei553PhosphothreonineCombined sources1
Modified residuei556PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40494
PaxDbiP40494
PRIDEiP40494

PTM databases

iPTMnetiP40494

Interactioni

Subunit structurei

Interacts with ABP1, which is required for proper actin patch localization.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34897, 168 interactors
DIPiDIP-6271N
IntActiP40494, 37 interactors
MINTiP40494
STRINGi4932.YIL095W

Structurei

3D structure databases

ProteinModelPortaliP40494
SMRiP40494
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 298Protein kinasePROSITE-ProRule annotationAdd BLAST277

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni743 – 756Interaction with SH3 domain of ABP1Add BLAST14

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00880000137958
HOGENOMiHOG000247884
InParanoidiP40494
KOiK08853
OMAiNANIACL
OrthoDBiEOG092C0MJY

Family and domain databases

InterProiView protein in InterPro
IPR030078 Ark1/Prk1
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR22967:SF82 PTHR22967:SF82, 1 hit
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P40494-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTPQISLYE PGTILTVGSH HAKIIKYLTS GGFAQVYTAE ISPPDPYSNA
60 70 80 90 100
NIACLKRVIV PHKQGLNTLR AEVDAMKLLR NNKHVVSYID SHAARSVNGI
110 120 130 140 150
AYEVFVLMEF CERGGLIDFM NTRLQNRLQE SEILEIMSQT VQGITAMHAL
160 170 180 190 200
QPPLIHRDIK IENVLISHDG LYKVCDFGSV SGVIRPPRNT QEFNYVQHDI
210 220 230 240 250
LTNTTAQYRS PEMIDLYRGL PIDEKSDIWA LGVFLYKICY YTTPFEKSGE
260 270 280 290 300
AGILHARYQY PSFPQYSDRL KNLIRLMLME APSQRPNICQ VLEEVSRLQN
310 320 330 340 350
KPCPIRNFYL LRAMNQNANT QLAGEPSSTT YVPTQKFIPV QSLQSINQPP
360 370 380 390 400
NMMPVTHVST TPNLGTFPIS INDNNKTEVT AHAGLQVGSH SNLTSPLMKT
410 420 430 440 450
KSVPLSDEFA SLYYKELHPF QKSQTFKSVE SFQSPQRKSM PPLSLTPVNN
460 470 480 490 500
DIFDRVSAIN RPNNYVDSET QTIDNMAVPN LKLSPTITSK SLSSTKEIAA
510 520 530 540 550
PDNINGSKIV RSLSSKLKKV ITGESRGNSP IKSRQNTGDS IRSAFGKLRH
560 570 580 590 600
GFTGNSVNNS RSASFDNNNV NGNGNNTNRR LVSSSTSSFP KFNSDTKRKE
610 620 630 640 650
ESDKNQRLEK RRSMPPSILS DFDQHERNNS RTGSRDYYRS HSPVKKTQAS
660 670 680 690 700
AKTTSKPTLI PDNGNVNINQ EKKESIQRRV HNLLKSSDDP VTYKSASGYG
710 720 730 740 750
KYTDIGTETS NRHSSVRITP ITEEKFKKTL KDGVLDIKTK SNGKDKSRPP
760 770 780 790 800
RPPPKPLHLR TEIQKIRNFS RLQSKKLPIE RISSEATETI VDVNVDDLEA
810
DFRKRFPSKV
Length:810
Mass (Da):91,032
Last modified:February 1, 1995 - v1
Checksum:iAF710F930B39BC7E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti786A → R in AAA86529 (PubMed:7597081).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24167 mRNA Translation: AAA86529.1
Z46728 Genomic DNA Translation: CAA86699.2
BK006942 Genomic DNA Translation: DAA08458.1
PIRiS50889
RefSeqiNP_012171.1, NM_001179443.1

Genome annotation databases

EnsemblFungiiYIL095W; YIL095W; YIL095W
GeneIDi854713
KEGGisce:YIL095W

Similar proteinsi

Entry informationi

Entry nameiPRK1_YEAST
AccessioniPrimary (citable) accession number: P40494
Secondary accession number(s): D6VVJ2, Q02553
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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