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P40494

- PRK1_YEAST

UniProt

P40494 - PRK1_YEAST

Protein

Actin-regulating kinase PRK1

Gene

PRK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Involved in regulation of actin cytoskeleton organization and endocytosis.3 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei56 – 561ATPPROSITE-ProRule annotation
    Active sitei158 – 1581Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi28 – 369ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein serine/threonine kinase activity Source: SGD

    GO - Biological processi

    1. actin cortical patch assembly Source: SGD
    2. actin filament organization Source: SGD
    3. protein phosphorylation Source: SGD
    4. regulation of endocytosis Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31354-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Actin-regulating kinase PRK1 (EC:2.7.11.1)
    Alternative name(s):
    p53-regulating kinase 1
    Gene namesi
    Name:PRK1
    Synonyms:PAK1
    Ordered Locus Names:YIL095W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL095w.
    SGDiS000001357. PRK1.

    Subcellular locationi

    Cytoplasmcytoskeletonactin patch 2 Publications
    Note: Cortical actin patches.

    GO - Cellular componenti

    1. actin cortical patch Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi56 – 561K → A: Abolishes protein kinase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 810810Actin-regulating kinase PRK1PRO_0000086581Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei402 – 4021Phosphoserine2 Publications
    Modified residuei428 – 4281Phosphoserine1 Publication
    Modified residuei484 – 4841Phosphoserine2 Publications
    Modified residuei553 – 5531Phosphothreonine1 Publication
    Modified residuei556 – 5561Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP40494.
    PaxDbiP40494.

    Expressioni

    Gene expression databases

    GenevestigatoriP40494.

    Interactioni

    Subunit structurei

    Interacts with ABP1, which is required for proper actin patch localization.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ABP1P1589110EBI-9703,EBI-2036
    PEX13P806672EBI-9703,EBI-13206

    Protein-protein interaction databases

    BioGridi34897. 139 interactions.
    DIPiDIP-6271N.
    IntActiP40494. 26 interactions.
    MINTiMINT-593513.
    STRINGi4932.YIL095W.

    Structurei

    3D structure databases

    ProteinModelPortaliP40494.
    SMRiP40494. Positions 12-318.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 298277Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni743 – 75614Interaction with SH3 domain of ABP1Add
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00510000046552.
    HOGENOMiHOG000247884.
    KOiK08853.
    OMAiNANIACL.
    OrthoDBiEOG74BK1F.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40494-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNTPQISLYE PGTILTVGSH HAKIIKYLTS GGFAQVYTAE ISPPDPYSNA    50
    NIACLKRVIV PHKQGLNTLR AEVDAMKLLR NNKHVVSYID SHAARSVNGI 100
    AYEVFVLMEF CERGGLIDFM NTRLQNRLQE SEILEIMSQT VQGITAMHAL 150
    QPPLIHRDIK IENVLISHDG LYKVCDFGSV SGVIRPPRNT QEFNYVQHDI 200
    LTNTTAQYRS PEMIDLYRGL PIDEKSDIWA LGVFLYKICY YTTPFEKSGE 250
    AGILHARYQY PSFPQYSDRL KNLIRLMLME APSQRPNICQ VLEEVSRLQN 300
    KPCPIRNFYL LRAMNQNANT QLAGEPSSTT YVPTQKFIPV QSLQSINQPP 350
    NMMPVTHVST TPNLGTFPIS INDNNKTEVT AHAGLQVGSH SNLTSPLMKT 400
    KSVPLSDEFA SLYYKELHPF QKSQTFKSVE SFQSPQRKSM PPLSLTPVNN 450
    DIFDRVSAIN RPNNYVDSET QTIDNMAVPN LKLSPTITSK SLSSTKEIAA 500
    PDNINGSKIV RSLSSKLKKV ITGESRGNSP IKSRQNTGDS IRSAFGKLRH 550
    GFTGNSVNNS RSASFDNNNV NGNGNNTNRR LVSSSTSSFP KFNSDTKRKE 600
    ESDKNQRLEK RRSMPPSILS DFDQHERNNS RTGSRDYYRS HSPVKKTQAS 650
    AKTTSKPTLI PDNGNVNINQ EKKESIQRRV HNLLKSSDDP VTYKSASGYG 700
    KYTDIGTETS NRHSSVRITP ITEEKFKKTL KDGVLDIKTK SNGKDKSRPP 750
    RPPPKPLHLR TEIQKIRNFS RLQSKKLPIE RISSEATETI VDVNVDDLEA 800
    DFRKRFPSKV 810
    Length:810
    Mass (Da):91,032
    Last modified:February 1, 1995 - v1
    Checksum:iAF710F930B39BC7E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti786 – 7861A → R in AAA86529. (PubMed:7597081)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U24167 mRNA. Translation: AAA86529.1.
    Z46728 Genomic DNA. Translation: CAA86699.2.
    BK006942 Genomic DNA. Translation: DAA08458.1.
    PIRiS50889.
    RefSeqiNP_012171.1. NM_001179443.1.

    Genome annotation databases

    EnsemblFungiiYIL095W; YIL095W; YIL095W.
    GeneIDi854713.
    KEGGisce:YIL095W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U24167 mRNA. Translation: AAA86529.1 .
    Z46728 Genomic DNA. Translation: CAA86699.2 .
    BK006942 Genomic DNA. Translation: DAA08458.1 .
    PIRi S50889.
    RefSeqi NP_012171.1. NM_001179443.1.

    3D structure databases

    ProteinModelPortali P40494.
    SMRi P40494. Positions 12-318.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34897. 139 interactions.
    DIPi DIP-6271N.
    IntActi P40494. 26 interactions.
    MINTi MINT-593513.
    STRINGi 4932.YIL095W.

    Proteomic databases

    MaxQBi P40494.
    PaxDbi P40494.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL095W ; YIL095W ; YIL095W .
    GeneIDi 854713.
    KEGGi sce:YIL095W.

    Organism-specific databases

    CYGDi YIL095w.
    SGDi S000001357. PRK1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00510000046552.
    HOGENOMi HOG000247884.
    KOi K08853.
    OMAi NANIACL.
    OrthoDBi EOG74BK1F.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31354-MONOMER.

    Miscellaneous databases

    NextBioi 977380.

    Gene expression databases

    Genevestigatori P40494.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Novel protein kinases Ark1p and Prk1p associate with and regulate the cortical actin cytoskeleton in budding yeast."
      Cope M.J.T.V., Yang S., Shang C., Drubin D.G.
      J. Cell Biol. 144:1203-1218(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-56.
    5. "In vivo role for actin-regulating kinases in endocytosis and yeast epsin phosphorylation."
      Watson H.A., Cope M.J.T.V., Groen A.C., Drubin D.G., Wendland B.
      Mol. Biol. Cell 12:3668-3679(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Regulation of yeast actin cytoskeleton-regulatory complex Pan1p/Sla1p/End3p by serine/threonine kinase Prk1p."
      Zeng G., Yu X., Cai M.
      Mol. Biol. Cell 12:3759-3772(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Unusual binding properties of the SH3 domain of the yeast actin-binding protein Abp1: structural and functional analysis."
      Fazi B., Cope M.J.T.V., Douangamath A., Ferracuti S., Schirwitz K., Zucconi A., Drubin D.G., Wilmanns M., Cesareni G., Castagnoli L.
      J. Biol. Chem. 277:5290-5298(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ABP1.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-402 AND SER-484, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-402; SER-428; SER-484; THR-553 AND SER-556, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPRK1_YEAST
    AccessioniPrimary (citable) accession number: P40494
    Secondary accession number(s): D6VVJ2, Q02553
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 136 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1323 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3