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Protein

Actin-regulating kinase PRK1

Gene

PRK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in regulation of actin cytoskeleton organization and endocytosis.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei56 – 561ATPPROSITE-ProRule annotation
Active sitei158 – 1581Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 369ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  1. actin cortical patch assembly Source: SGD
  2. actin filament organization Source: SGD
  3. protein phosphorylation Source: SGD
  4. regulation of endocytosis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31354-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-regulating kinase PRK1 (EC:2.7.11.1)
Alternative name(s):
p53-regulating kinase 1
Gene namesi
Name:PRK1
Synonyms:PAK1
Ordered Locus Names:YIL095W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIL095w.
SGDiS000001357. PRK1.

Subcellular locationi

Cytoplasmcytoskeletonactin patch 2 Publications
Note: Cortical actin patches.

GO - Cellular componenti

  1. actin cortical patch Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi56 – 561K → A: Abolishes protein kinase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 810810Actin-regulating kinase PRK1PRO_0000086581Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei402 – 4021Phosphoserine2 Publications
Modified residuei428 – 4281Phosphoserine1 Publication
Modified residuei484 – 4841Phosphoserine2 Publications
Modified residuei553 – 5531Phosphothreonine1 Publication
Modified residuei556 – 5561Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40494.
PaxDbiP40494.

Expressioni

Gene expression databases

GenevestigatoriP40494.

Interactioni

Subunit structurei

Interacts with ABP1, which is required for proper actin patch localization.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ABP1P1589110EBI-9703,EBI-2036
PEX13P806672EBI-9703,EBI-13206

Protein-protein interaction databases

BioGridi34897. 139 interactions.
DIPiDIP-6271N.
IntActiP40494. 26 interactions.
MINTiMINT-593513.
STRINGi4932.YIL095W.

Structurei

3D structure databases

ProteinModelPortaliP40494.
SMRiP40494. Positions 14-357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 298277Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni743 – 75614Interaction with SH3 domain of ABP1Add
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00510000046552.
HOGENOMiHOG000247884.
InParanoidiP40494.
KOiK08853.
OMAiNANIACL.
OrthoDBiEOG74BK1F.

Family and domain databases

InterProiIPR030078. Ark1/Prk1.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR22967:SF59. PTHR22967:SF59. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40494-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNTPQISLYE PGTILTVGSH HAKIIKYLTS GGFAQVYTAE ISPPDPYSNA
60 70 80 90 100
NIACLKRVIV PHKQGLNTLR AEVDAMKLLR NNKHVVSYID SHAARSVNGI
110 120 130 140 150
AYEVFVLMEF CERGGLIDFM NTRLQNRLQE SEILEIMSQT VQGITAMHAL
160 170 180 190 200
QPPLIHRDIK IENVLISHDG LYKVCDFGSV SGVIRPPRNT QEFNYVQHDI
210 220 230 240 250
LTNTTAQYRS PEMIDLYRGL PIDEKSDIWA LGVFLYKICY YTTPFEKSGE
260 270 280 290 300
AGILHARYQY PSFPQYSDRL KNLIRLMLME APSQRPNICQ VLEEVSRLQN
310 320 330 340 350
KPCPIRNFYL LRAMNQNANT QLAGEPSSTT YVPTQKFIPV QSLQSINQPP
360 370 380 390 400
NMMPVTHVST TPNLGTFPIS INDNNKTEVT AHAGLQVGSH SNLTSPLMKT
410 420 430 440 450
KSVPLSDEFA SLYYKELHPF QKSQTFKSVE SFQSPQRKSM PPLSLTPVNN
460 470 480 490 500
DIFDRVSAIN RPNNYVDSET QTIDNMAVPN LKLSPTITSK SLSSTKEIAA
510 520 530 540 550
PDNINGSKIV RSLSSKLKKV ITGESRGNSP IKSRQNTGDS IRSAFGKLRH
560 570 580 590 600
GFTGNSVNNS RSASFDNNNV NGNGNNTNRR LVSSSTSSFP KFNSDTKRKE
610 620 630 640 650
ESDKNQRLEK RRSMPPSILS DFDQHERNNS RTGSRDYYRS HSPVKKTQAS
660 670 680 690 700
AKTTSKPTLI PDNGNVNINQ EKKESIQRRV HNLLKSSDDP VTYKSASGYG
710 720 730 740 750
KYTDIGTETS NRHSSVRITP ITEEKFKKTL KDGVLDIKTK SNGKDKSRPP
760 770 780 790 800
RPPPKPLHLR TEIQKIRNFS RLQSKKLPIE RISSEATETI VDVNVDDLEA
810
DFRKRFPSKV
Length:810
Mass (Da):91,032
Last modified:February 1, 1995 - v1
Checksum:iAF710F930B39BC7E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti786 – 7861A → R in AAA86529. (PubMed:7597081)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24167 mRNA. Translation: AAA86529.1.
Z46728 Genomic DNA. Translation: CAA86699.2.
BK006942 Genomic DNA. Translation: DAA08458.1.
PIRiS50889.
RefSeqiNP_012171.1. NM_001179443.1.

Genome annotation databases

EnsemblFungiiYIL095W; YIL095W; YIL095W.
GeneIDi854713.
KEGGisce:YIL095W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24167 mRNA. Translation: AAA86529.1.
Z46728 Genomic DNA. Translation: CAA86699.2.
BK006942 Genomic DNA. Translation: DAA08458.1.
PIRiS50889.
RefSeqiNP_012171.1. NM_001179443.1.

3D structure databases

ProteinModelPortaliP40494.
SMRiP40494. Positions 14-357.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34897. 139 interactions.
DIPiDIP-6271N.
IntActiP40494. 26 interactions.
MINTiMINT-593513.
STRINGi4932.YIL095W.

Proteomic databases

MaxQBiP40494.
PaxDbiP40494.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL095W; YIL095W; YIL095W.
GeneIDi854713.
KEGGisce:YIL095W.

Organism-specific databases

CYGDiYIL095w.
SGDiS000001357. PRK1.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00510000046552.
HOGENOMiHOG000247884.
InParanoidiP40494.
KOiK08853.
OMAiNANIACL.
OrthoDBiEOG74BK1F.

Enzyme and pathway databases

BioCyciYEAST:G3O-31354-MONOMER.

Miscellaneous databases

NextBioi977380.

Gene expression databases

GenevestigatoriP40494.

Family and domain databases

InterProiIPR030078. Ark1/Prk1.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR22967:SF59. PTHR22967:SF59. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Novel protein kinases Ark1p and Prk1p associate with and regulate the cortical actin cytoskeleton in budding yeast."
    Cope M.J.T.V., Yang S., Shang C., Drubin D.G.
    J. Cell Biol. 144:1203-1218(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-56.
  5. "In vivo role for actin-regulating kinases in endocytosis and yeast epsin phosphorylation."
    Watson H.A., Cope M.J.T.V., Groen A.C., Drubin D.G., Wendland B.
    Mol. Biol. Cell 12:3668-3679(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Regulation of yeast actin cytoskeleton-regulatory complex Pan1p/Sla1p/End3p by serine/threonine kinase Prk1p."
    Zeng G., Yu X., Cai M.
    Mol. Biol. Cell 12:3759-3772(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Unusual binding properties of the SH3 domain of the yeast actin-binding protein Abp1: structural and functional analysis."
    Fazi B., Cope M.J.T.V., Douangamath A., Ferracuti S., Schirwitz K., Zucconi A., Drubin D.G., Wilmanns M., Cesareni G., Castagnoli L.
    J. Biol. Chem. 277:5290-5298(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ABP1.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-402 AND SER-484, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-402; SER-428; SER-484; THR-553 AND SER-556, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPRK1_YEAST
AccessioniPrimary (citable) accession number: P40494
Secondary accession number(s): D6VVJ2, Q02553
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: January 7, 2015
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1323 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.