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Protein

25S rRNA (uridine(2634)-N(3))-methyltransferase

Gene

BMT5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N3 position of uridine 2634 (m3U2634) in 25S rRNA.1 Publication

Miscellaneous

Present with 2960 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + uracil(2634) in 25S rRNA = S-adenosyl-L-homocysteine + N3-methyluracil(2634) in 25S rRNA.1 Publication

GO - Molecular functioni

  • rRNA (uridine-N3-)-methyltransferase activity Source: SGD
  • S-adenosylmethionine-dependent methyltransferase activity Source: SGD

GO - Biological processi

  • methylation Source: SGD
  • rRNA base methylation Source: SGD

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:G3O-31355-MONOMER
YEAST:G3O-31355-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
25S rRNA (uridine(2634)-N(3))-methyltransferase (EC:2.1.1.3131 Publication)
Alternative name(s):
Base methyltransferase of 25S RNA 5
Gene namesi
Name:BMT5
Ordered Locus Names:YIL096C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL096C
SGDiS000001358 BMT5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi182G → R: Abolishes methyltransferase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002029711 – 33625S rRNA (uridine(2634)-N(3))-methyltransferaseAdd BLAST336

Proteomic databases

MaxQBiP40493
PaxDbiP40493
PRIDEiP40493

Interactioni

Protein-protein interaction databases

BioGridi34895, 46 interactors
DIPiDIP-5098N
IntActiP40493, 1 interactor
MINTiP40493
STRINGi4932.YIL096C

Structurei

3D structure databases

ProteinModelPortaliP40493
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000000965
InParanoidiP40493
KOiK19307
OMAiGGEDRTW
OrthoDBiEOG092C3Z33

Family and domain databases

InterProiView protein in InterPro
IPR019446 DUF2431
PfamiView protein in Pfam
PF10354 DUF2431, 1 hit

Sequencei

Sequence statusi: Complete.

P40493-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARKLKGKIG SKGLKGALLR HKAKVKLVRN IESKQKHELR KKNSSANNKT
60 70 80 90 100
VKRNQEFQKL NQGKVMPFEK DETLMLCGEG DFSFARSIVE QNYIESDNLI
110 120 130 140 150
ITSYDNSVNE LKLKYPHTFE ENYQYLKDLN IPIFFQIDVT KLVKSFKISK
160 170 180 190 200
NNTWFKIINR LSDHRWGNKP LQNIVFNFPH NGKGIKDQER NIREHQDLIF
210 220 230 240 250
NFFQNSLQLF NLINTKIQND TLRYTQGYDL NEDTPQAKKL TAEGYGNIIL
260 270 280 290 300
SLFDGEPYDS WQIKLLAKKN GLTLSRSSKF QWENFPGYHH RRTNSEQDTT
310 320 330
KPAKERDARF YIFSKYVSNS SKHNRKSKKD TDSDSD
Length:336
Mass (Da):39,402
Last modified:February 1, 1995 - v1
Checksum:iC36EC3034A746A11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38125 Genomic DNA Translation: CAA86285.1
BK006942 Genomic DNA Translation: DAA08457.1
PIRiS48477
RefSeqiNP_012170.1, NM_001179444.1

Genome annotation databases

EnsemblFungiiYIL096C; YIL096C; YIL096C
GeneIDi854711
KEGGisce:YIL096C

Similar proteinsi

Entry informationi

Entry nameiBMT5_YEAST
AccessioniPrimary (citable) accession number: P40493
Secondary accession number(s): D6VVJ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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