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Protein

25S rRNA (uridine(2634)-N(3))-methyltransferase

Gene

BMT5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N3 position of uridine 2634 (m3U2634) in 25S rRNA.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + uracil(2634) in 25S rRNA = S-adenosyl-L-homocysteine + N(3)-methyluracil(2634) in 25S rRNA.1 Publication

GO - Molecular functioni

  • rRNA (uridine-N3-)-methyltransferase activity Source: SGD
  • S-adenosylmethionine-dependent methyltransferase activity Source: SGD

GO - Biological processi

  • methylation Source: SGD
  • rRNA base methylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-31355-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
25S rRNA (uridine(2634)-N(3))-methyltransferase (EC:2.1.1.3131 Publication)
Alternative name(s):
Base methyltransferase of 25S RNA 5
Gene namesi
Name:BMT5
Ordered Locus Names:YIL096C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL096C.
SGDiS000001358. BMT5.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi182 – 1821G → R: Abolishes methyltransferase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 33633625S rRNA (uridine(2634)-N(3))-methyltransferasePRO_0000202971Add
BLAST

Proteomic databases

MaxQBiP40493.

Interactioni

Protein-protein interaction databases

BioGridi34895. 22 interactions.
DIPiDIP-5098N.
IntActiP40493. 1 interaction.
MINTiMINT-491772.

Structurei

3D structure databases

ProteinModelPortaliP40493.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000000965.
InParanoidiP40493.
KOiK19307.
OMAiDRTWEFC.
OrthoDBiEOG74FFB1.

Family and domain databases

InterProiIPR019446. DUF2431.
[Graphical view]
PfamiPF10354. DUF2431. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40493-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARKLKGKIG SKGLKGALLR HKAKVKLVRN IESKQKHELR KKNSSANNKT
60 70 80 90 100
VKRNQEFQKL NQGKVMPFEK DETLMLCGEG DFSFARSIVE QNYIESDNLI
110 120 130 140 150
ITSYDNSVNE LKLKYPHTFE ENYQYLKDLN IPIFFQIDVT KLVKSFKISK
160 170 180 190 200
NNTWFKIINR LSDHRWGNKP LQNIVFNFPH NGKGIKDQER NIREHQDLIF
210 220 230 240 250
NFFQNSLQLF NLINTKIQND TLRYTQGYDL NEDTPQAKKL TAEGYGNIIL
260 270 280 290 300
SLFDGEPYDS WQIKLLAKKN GLTLSRSSKF QWENFPGYHH RRTNSEQDTT
310 320 330
KPAKERDARF YIFSKYVSNS SKHNRKSKKD TDSDSD
Length:336
Mass (Da):39,402
Last modified:February 1, 1995 - v1
Checksum:iC36EC3034A746A11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38125 Genomic DNA. Translation: CAA86285.1.
BK006942 Genomic DNA. Translation: DAA08457.1.
PIRiS48477.
RefSeqiNP_012170.1. NM_001179444.1.

Genome annotation databases

EnsemblFungiiYIL096C; YIL096C; YIL096C.
GeneIDi854711.
KEGGisce:YIL096C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38125 Genomic DNA. Translation: CAA86285.1.
BK006942 Genomic DNA. Translation: DAA08457.1.
PIRiS48477.
RefSeqiNP_012170.1. NM_001179444.1.

3D structure databases

ProteinModelPortaliP40493.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34895. 22 interactions.
DIPiDIP-5098N.
IntActiP40493. 1 interaction.
MINTiMINT-491772.

Proteomic databases

MaxQBiP40493.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL096C; YIL096C; YIL096C.
GeneIDi854711.
KEGGisce:YIL096C.

Organism-specific databases

EuPathDBiFungiDB:YIL096C.
SGDiS000001358. BMT5.

Phylogenomic databases

HOGENOMiHOG000000965.
InParanoidiP40493.
KOiK19307.
OMAiDRTWEFC.
OrthoDBiEOG74FFB1.

Enzyme and pathway databases

BioCyciYEAST:G3O-31355-MONOMER.

Miscellaneous databases

PROiP40493.

Family and domain databases

InterProiIPR019446. DUF2431.
[Graphical view]
PfamiPF10354. DUF2431. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  4. "Comprehensive structural and substrate specificity classification of the Saccharomyces cerevisiae methyltransferome."
    Wlodarski T., Kutner J., Towpik J., Knizewski L., Rychlewski L., Kudlicki A., Rowicka M., Dziembowski A., Ginalski K.
    PLoS ONE 6:E23168-E23168(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION AS METHYLTRANSFERASE, SAM-BINDING.
  5. "Identification of novel methyltransferases, Bmt5 and Bmt6, responsible for the m3U methylations of 25S rRNA in Saccharomyces cerevisiae."
    Sharma S., Yang J., Duttmann S., Watzinger P., Kotter P., Entian K.D.
    Nucleic Acids Res. 42:3246-3260(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, SAM-BINDING, MUTAGENESIS OF GLY-182.

Entry informationi

Entry nameiBMT5_YEAST
AccessioniPrimary (citable) accession number: P40493
Secondary accession number(s): D6VVJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 8, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2960 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.